FvH4_7g33080

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006698
Physical Location & Seq
Reverse (-)
23629703 .. 23633502
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g33080.t5

Sequence Viewer

Length: 1332 bp
ATGACGAAGATGATGAAGGGTAGGGTGTCTAGAAATGGAAGCTATGCAATTGCTTCGTCTATGAAAGATGGACATGATCAACAGCCTTGCATTACCTGCACCACATTCAACATCCTTGCTCCAATCTACAAGCGCCTAAGCCATGAGGACCCTGGTTGTAGGGAAAGCGATCACAGGGCTTATTGGCTGGCCAGAAATCACATGATTTTGGATTGTTTGTTGGCTGAGAGATCGTCCATTATTTGTCTTCAGGAGTTCTGGGTTGGGAATGAAGAGCTTGTGAACATTTATGAGAAGAGACTTGGTGATGCTGGTTATATCAATTTTAAGCTAGGAAGGACAAACAACCGCGGTGATGGTTTGCTGACTGCAGTGCATAAGGACTACTTTAGAGTTATAAATTATCAAGAGGTGCACTTCAATGATTGTGGAGATCGTGTTGCTCAGTTGTTACATGTTGAATTAGCTTCCCCAGTTTCACAATTTCGAAACAATGATATTCGGCAGGAAATGCTCATTGTGAATACCCATTTGTTATTTCCTCATGATTCAAGTTTATGTACTGTACGATTGCGTCAGGTGTACAAAATCCTACAATATGTGGAATCTTATCAGAAAAAGAACATACTTAACCCCATGCCCATCATTCTCTGTGGTGATTGGAATGGAAGCAAACGTGGACATGTTTACAAGTTCCTTAGGTCCCAGGGGTTTGTATCATCTTATGATACTGCTCATCATTACACCGATGCAGATGCACACAAGTGGGTTAGCCACCGAAACCATCGAGGAAACATATGTGGGGTTGACTTTATATGGCTTCTTAACCCAAATAGTTACCGGAAACTACTAAAAACAAGTTGGAGTGAAGCAGTATTTGGCATGTTCAAGTATCTACTACGAAGAGCTTCACTGACAGAAAGTGATGCCTTCGCCTTTTTGAAGGCTGACAACCCGGGTGATTACATTACTTATTCTGACTTCTGTGAAGCCCTCCGACAGCTTAATTTGACTGGTCATTGCAATGGACTTAGCCATGAAGAGACCAAGGACTTATGGGTTCAAGCAGACATAGATGGCAATGGTATTCTTGATTACGAAGGGTTTCAGCAGCGAATTTGGAATCTAACAGATGGTGGTAATGAGATCTGGGATGTTGGCTCTAATGGTATTCAGGAGCAAGCTATTGGTTTCAGTGTAAAAAATGCAGTTCTTTTCCCTCCTGAAGTGGAGAAAGGAACGTGGCCTGAAGACTACTCTCTTTCTGATCATGCACGACTAACTGTGGTTTTCTCACCGATAAGAATGCCATGCTCTCAGATGATCGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

444

Amino Acids

50.84

Weight (kDa)

6.52

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 206 1.1e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 104
AccII CGCG 1 cut(s) 351
AciI CCGC 2 cut(s) 349, 351
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 1 cut(s) 1116
AcuI CTGAAG 3 cut(s) 233, 1245, 1269
AfaI GTAC 3 cut(s) 562, 567, 584
AflIII ACRYGT 2 cut(s) 454, 682
AgsI TTSAA 7 cut(s) 109, 421, 461, 552, 889, 943, 1064
AjnI CCWGG 2 cut(s) 151, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 861, 893
AleI CACNNNNGTG 1 cut(s) 763
AluBI AGCT 7 cut(s) 42, 277, 331, 467, 908, 1003, 1184
AluI AGCT 7 cut(s) 42, 277, 331, 467, 908, 1003, 1184
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 292, 1037
Alw44I GTGCAC 1 cut(s) 413
Ama87I CYCGRG 1 cut(s) 955
AoxI GGCC 2 cut(s) 189, 1244
ApaLI GTGCAC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 1111
ApoI RAATTY 1 cut(s) 1116
Asp700I GAANNNNTTC 2 cut(s) 907, 1104
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 2 cut(s) 148, 702
AsuC2I CCSGG 2 cut(s) 956, 957
AsuHPI GGTGA 5 cut(s) 317, 365, 668, 971, 1287
AsuII TTCGAA 1 cut(s) 487
AvaI CYCGRG 1 cut(s) 955
AvaII GGWCC 2 cut(s) 148, 702
AxyI CCTNAGG 1 cut(s) 698
BaeGI GKGCMC 1 cut(s) 417
BalI TGGCCA 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 239, 1257
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 1123
BccI CCATC 6 cut(s) 62, 350, 650, 792, 1070, 1127
BcgI CGANNNNNNTGC 6 cut(s) 36, 70, 737, 771, 1288, 1322
BciT130I CCWGG 2 cut(s) 153, 707
BclI TGATCA 2 cut(s) 76, 1267
BcnI CCSGG 2 cut(s) 956, 957
BcoDI GTCTC 2 cut(s) 292, 1037
BfaI CTAG 2 cut(s) 30, 332
BfmI CTRYAG 1 cut(s) 369
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 1146
BisI GCNGC 1 cut(s) 1112
BlsI GCNGC 1 cut(s) 1113
Bme1390I CCNGG 4 cut(s) 153, 707, 956, 957
Bme18I GGWCC 2 cut(s) 148, 702
BmeT110I CYCGRG 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 148, 702
BmiI GGNNCC 2 cut(s) 150, 704
BmrFI CCNGG 4 cut(s) 153, 707, 956, 957
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 4 cut(s) 298, 739, 745, 916
BmuI ACTGGG 1 cut(s) 467
BpiI GAAGAC 2 cut(s) 239, 1257
Bpu10I CCTNAGC 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 487
BpuMI CCSGG 2 cut(s) 956, 957
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 6 cut(s) 151, 349, 705, 706, 955, 1047
BsaWI WCCGGW 1 cut(s) 840
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bse1I ACTGG 2 cut(s) 473, 1018
Bse21I CCTNAGG 1 cut(s) 698
Bse3DI GCAATG 3 cut(s) 1018, 1030, 1087
BseBI CCWGG 2 cut(s) 153, 707
BseDI CCNNGG 6 cut(s) 151, 349, 705, 706, 955, 1047
BseGI GGATG 2 cut(s) 111, 1159
BseMI GCAATG 3 cut(s) 1018, 1030, 1087
BseMII CTCAG 3 cut(s) 216, 458, 1331
BseNI ACTGG 2 cut(s) 473, 1018
BseSI GKGCMC 1 cut(s) 417
BseXI GCAGC 1 cut(s) 1123
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 191, 1246
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 955
BsiSI CCGG 2 cut(s) 841, 956
BslFI GGGAC 1 cut(s) 688
BsmAI GTCTC 2 cut(s) 292, 1037
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 1311
BsnI GGCC 2 cut(s) 191, 1246
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 1 cut(s) 955
Bsp119I TTCGAA 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 417
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
BspACI CCGC 2 cut(s) 349, 351
BspANI GGCC 2 cut(s) 191, 1246
BspCNI CTCAG 3 cut(s) 217, 457, 1330
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 150, 704
BspMAI CTGCAG 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 104
BspQI GCTCTTC 2 cut(s) 267, 898
BspT104I TTCGAA 1 cut(s) 487
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 3 cut(s) 1018, 1030, 1087
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 2 cut(s) 473, 1018
BssECI CCNNGG 6 cut(s) 151, 349, 705, 706, 955, 1047
BssMI GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
BssT1I CCWWGG 1 cut(s) 1047
Bst2UI CCWGG 2 cut(s) 153, 707
Bst4CI ACNGT 2 cut(s) 565, 1285
Bst6I CTCTTC 4 cut(s) 267, 290, 898, 1035
BstAPI GCANNNNNTGC 2 cut(s) 96, 511
BstAUI TGTACA 1 cut(s) 582
BstBI TTCGAA 1 cut(s) 487
BstC8I GCNNGC 2 cut(s) 189, 1182
BstDEI CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
BstDSI CCRYGG 1 cut(s) 349
BstF5I GGATG 2 cut(s) 111, 1159
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 7 cut(s) 79, 172, 233, 436, 1149, 1270, 1326
BstMAI GTCTC 2 cut(s) 292, 1037
BstMBI GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
BstMWI GCNNNNNNNGC 2 cut(s) 96, 511
BstNI CCWGG 2 cut(s) 153, 707
BstNSI RCATGY 3 cut(s) 458, 686, 886
BstSCI CCNGG 4 cut(s) 151, 705, 954, 955
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 1123
BstV2I GAAGAC 2 cut(s) 239, 1257
BstX2I RGATCY 1 cut(s) 1146
BstYI RGATCY 1 cut(s) 1146
Bsu36I CCTNAGG 1 cut(s) 698
BsuRI GGCC 2 cut(s) 191, 1246
BtgI CCRYGG 1 cut(s) 349
BtsCI GGATG 2 cut(s) 111, 1159
BtsI GCAGTG 1 cut(s) 378
BtsIMutI CAGTG 3 cut(s) 378, 911, 1201
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 189, 1182
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 2 cut(s) 148, 702
Cfr42I CCGCGG 1 cut(s) 352
Cfr9I CCCGGG 1 cut(s) 955
CseI GACGC 1 cut(s) 563
Csp6I GTAC 3 cut(s) 561, 566, 583
CspCI CAANNNNNGTGG 2 cut(s) 409, 444
CviQI GTAC 3 cut(s) 561, 566, 583
DdeI CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
DpnI GATC 7 cut(s) 78, 171, 232, 435, 1148, 1269, 1325
DpnII GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 4 cut(s) 267, 290, 898, 1035
EarI CTCTTC 4 cut(s) 267, 290, 898, 1035
Eco130I CCWWGG 1 cut(s) 1047
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 148, 702
Eco57I CTGAAG 3 cut(s) 233, 1245, 1269
Eco81I CCTNAGG 1 cut(s) 698
Eco88I CYCGRG 1 cut(s) 955
EcoO109I RGGNCCY 2 cut(s) 148, 702
EcoRII CCWGG 2 cut(s) 151, 705
EcoT14I CCWWGG 1 cut(s) 1047
ErhI CCWWGG 1 cut(s) 1047
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 688
FauNDI CATATG 1 cut(s) 797
FbaI TGATCA 2 cut(s) 76, 1267
Fnu4HI GCNGC 1 cut(s) 1112
FokI GGATG 2 cut(s) 98, 1166
Fsp4HI GCNGC 1 cut(s) 1112
FspBI CTAG 2 cut(s) 30, 332
GlaI GCGC 1 cut(s) 134
GluI GCNGC 1 cut(s) 1112
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 2 cut(s) 191, 1246
HapII CCGG 2 cut(s) 841, 956
HgaI GACGC 1 cut(s) 563
HhaI GCGC 1 cut(s) 135
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HinfI GANTC 3 cut(s) 548, 605, 1123
HpaII CCGG 2 cut(s) 841, 956
HphI GGTGA 5 cut(s) 317, 365, 668, 971, 1287
Hpy166II GTNNAC 6 cut(s) 283, 415, 583, 680, 688, 808
Hpy188I TCNGA 5 cut(s) 615, 979, 998, 1267, 1320
Hpy188III TCNNGA 7 cut(s) 30, 251, 407, 545, 1091, 1175, 1223
Hpy8I GTNNAC 6 cut(s) 283, 415, 583, 680, 688, 808
HpyAV CCTTC 5 cut(s) 10, 330, 937, 940, 1094
HpyCH4III ACNGT 2 cut(s) 565, 1285
HpyCH4IV ACGT 2 cut(s) 676, 1241
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 511
HpyF3I CTNAG 6 cut(s) 137, 225, 444, 698, 1031, 1317
HpySE526I ACGT 2 cut(s) 676, 1241
HspAI GCGC 1 cut(s) 133
Ksp22I TGATCA 2 cut(s) 76, 1267
KspI CCGCGG 1 cut(s) 352
Kzo9I GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
LguI GCTCTTC 2 cut(s) 267, 898
LmnI GCTCC 2 cut(s) 124, 1177
Lsp1109I GCAGC 1 cut(s) 1123
LweI GCATC 4 cut(s) 298, 739, 745, 916
MaeI CTAG 2 cut(s) 30, 332
MaeII ACGT 2 cut(s) 676, 1241
MaeIII GTNAC 2 cut(s) 450, 836
MalI GATC 7 cut(s) 78, 171, 232, 435, 1148, 1269, 1325
MboI GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
MboII GAAGA 7 cut(s) 19, 239, 284, 307, 915, 1052, 1262
MfeI CAATTG 1 cut(s) 48
MflI RGATCY 1 cut(s) 1146
MhlI GDGCHC 1 cut(s) 417
MlsI TGGCCA 1 cut(s) 191
MluCI AATT 7 cut(s) 48, 322, 400, 461, 482, 1006, 1116
MluNI TGGCCA 1 cut(s) 191
MmeI TCCRAC 2 cut(s) 842, 1021
MnlI CCTC 6 cut(s) 139, 403, 552, 782, 1004, 1230
Mox20I TGGCCA 1 cut(s) 191
MroXI GAANNNNTTC 2 cut(s) 907, 1104
MscI TGGCCA 1 cut(s) 191
MseI TTAA 4 cut(s) 327, 630, 825, 1005
MslI CAYNNNNRTG 3 cut(s) 420, 763, 1023
Msp20I TGGCCA 1 cut(s) 191
MspA1I CMGCKG 1 cut(s) 351
MspI CCGG 2 cut(s) 841, 956
MspR9I CCNGG 4 cut(s) 153, 707, 956, 957
MunI CAATTG 1 cut(s) 48
Mva1269I GAATGC 1 cut(s) 1311
MvaI CCWGG 2 cut(s) 153, 707
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 96, 511
NciI CCSGG 2 cut(s) 956, 957
NdeI CATATG 1 cut(s) 797
NdeII GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
NlaIV GGNNCC 2 cut(s) 150, 704
NspI RCATGY 3 cut(s) 458, 686, 886
NspV TTCGAA 1 cut(s) 487
OliI CACNNNNGTG 1 cut(s) 763
PagI TCATGA 1 cut(s) 544
PasI CCCWGGG 1 cut(s) 706
PciI ACATGT 2 cut(s) 454, 682
PciSI GCTCTTC 2 cut(s) 267, 898
PctI GAATGC 1 cut(s) 1311
PdmI GAANNNNTTC 2 cut(s) 907, 1104
PfeI GAWTC 3 cut(s) 548, 605, 1123
PkrI GCNGC 1 cut(s) 1113
PpuMI RGGWCCY 2 cut(s) 148, 702
PscI ACATGT 2 cut(s) 454, 682
PsiI TTATAA 1 cut(s) 398
Psp5II RGGWCCY 2 cut(s) 148, 702
Psp6I CCWGG 2 cut(s) 151, 705
PspGI CCWGG 2 cut(s) 151, 705
PspN4I GGNNCC 2 cut(s) 150, 704
PspPI GGNCC 2 cut(s) 148, 702
PspPPI RGGWCCY 2 cut(s) 148, 702
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 1146
RsaI GTAC 3 cut(s) 562, 567, 584
RsaNI GTAC 3 cut(s) 561, 566, 583
RseI CAYNNNNRTG 3 cut(s) 420, 763, 1023
SacII CCGCGG 1 cut(s) 352
SapI GCTCTTC 2 cut(s) 267, 898
SaqAI TTAA 4 cut(s) 327, 630, 825, 1005
SatI GCNGC 1 cut(s) 1112
Sau3AI GATC 7 cut(s) 76, 169, 230, 433, 1146, 1267, 1323
Sau96I GGNCC 2 cut(s) 148, 702
ScrFI CCNGG 4 cut(s) 153, 707, 956, 957
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 4 cut(s) 298, 739, 745, 916
SfcI CTRYAG 1 cut(s) 369
Sfr303I CCGCGG 1 cut(s) 352
SfuI TTCGAA 1 cut(s) 487
SgrBI CCGCGG 1 cut(s) 352
SinI GGWCC 2 cut(s) 148, 702
SmaI CCCGGG 1 cut(s) 957
SmiMI CAYNNNNRTG 3 cut(s) 420, 763, 1023
Sse9I AATT 7 cut(s) 48, 322, 400, 461, 482, 1006, 1116
SsiI CCGC 2 cut(s) 349, 351
SspMI CTAG 2 cut(s) 30, 332
StyD4I CCNGG 4 cut(s) 151, 705, 954, 955
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 2 cut(s) 565, 1285
TaiI ACGT 2 cut(s) 679, 1244
TaqI TCGA 2 cut(s) 487, 787
TasI AATT 7 cut(s) 48, 322, 400, 461, 482, 1006, 1116
TatI WGTACW 2 cut(s) 560, 582
TfiI GAWTC 3 cut(s) 548, 605, 1123
Tru1I TTAA 4 cut(s) 327, 630, 825, 1005
Tru9I TTAA 4 cut(s) 327, 630, 825, 1005
TscAI CASTG 3 cut(s) 378, 918, 1201
TseI GCWGC 1 cut(s) 1111
TspDTI ATGAA 4 cut(s) 29, 77, 285, 1053
TspMI CCCGGG 1 cut(s) 955
TspRI CASTG 3 cut(s) 378, 918, 1201
VneI GTGCAC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 148, 702
XapI RAATTY 1 cut(s) 1116
XbaI TCTAGA 1 cut(s) 29
XceI RCATGY 3 cut(s) 458, 686, 886
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 955
XmnI GAANNNNTTC 2 cut(s) 907, 1104
XspI CTAG 2 cut(s) 30, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.