AT5G55110

stigma-specific Stig1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015225
Physical Location & Seq
Forward (+)
22367733 .. 22368675
943 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55110.1

Sequence Viewer

Length: 492 bp
ATGGCCCATTTCATGAAGCTACTCGTGACAATTGCATTAACAACCGTGGTCACAATCGCCATCGTCACCACCAAAACCACCACCAAGACCGCAACATTTGCTCTCGAAGACCCTTTCAAGGACCTTACACCACCAGGAACGATCAAGATCAGACCAAGTCGGTTCTTGGCAGAAAAAGTCGACAAGGGTAAAGAACCAAAAGCCCGTAACCCAAACGCAGCTGACCATTGCAACAAGGACAATGAAATCTGTAGCAGCAGCGATTACAGCACAGGAGCAAACGCTACAATGGCTTGTTGCAACAACAAGTGTATGGATTTATCAACCGACGACAAAAACTGCGGTTTATGTAAGAACAAATGCAAGTTCGGGCAAACGTGTTGTCGTGGTCAGTGCGTTTACGTGGCCTACGATAAACGCCATTGCGGTGAGTGTAACCATCGTTGCGATCTCGGCGAGTTCTGCGTCTACGGTCTCTGTAACTACGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

163

Amino Acids

17.92

Weight (kDa)

8.44

Isoelectric Point (pI)

27.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 75 - 163 5.2e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 180, 468
AccII CGCG 1 cut(s) 488
AciI CCGC 3 cut(s) 90, 342, 426
AfiI CCNNNNNNNGG 1 cut(s) 118
AflIII ACRYGT 2 cut(s) 377, 486
AgsI TTSAA 1 cut(s) 118
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 2 cut(s) 19, 221
AluI AGCT 2 cut(s) 19, 221
Alw26I GTCTC 1 cut(s) 479
AoxI GGCC 2 cut(s) 3, 405
ApeKI GCWGC 3 cut(s) 218, 255, 258
ArsI GACNNNNNNTTYG 2 cut(s) 367, 399
AspS9I GGNCC 2 cut(s) 4, 121
AsuHPI GGTGA 2 cut(s) 58, 440
AvaII GGWCC 1 cut(s) 121
BauI CACGAG 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 230, 267, 270
BccI CCATC 2 cut(s) 68, 447
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 479
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 3 cut(s) 219, 256, 259
BlsI GCNGC 3 cut(s) 220, 257, 260
Bme1390I CCNGG 1 cut(s) 135
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 2 cut(s) 4, 121
BmrFI CCNGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 118
Bse3DI GCAATG 2 cut(s) 226, 421
Bse8I GATNNNNATC 1 cut(s) 146
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 45
BseJI GATNNNNATC 1 cut(s) 146
BseLI CCNNNNNNNGG 1 cut(s) 118
BseMI GCAATG 2 cut(s) 226, 421
BseXI GCAGC 3 cut(s) 230, 267, 270
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 2 cut(s) 5, 407
BslI CCNNNNNNNGG 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 479
BsnI GGCC 2 cut(s) 5, 407
Bso31I GGTCTC 1 cut(s) 479
Bsp143I GATC 3 cut(s) 141, 147, 448
BspACI CCGC 3 cut(s) 90, 342, 426
BspANI GGCC 2 cut(s) 5, 407
BspFNI CGCG 1 cut(s) 488
BspHI TCATGA 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 479
BsrDI GCAATG 2 cut(s) 226, 421
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 3 cut(s) 141, 147, 448
BssSI CACGAG 1 cut(s) 23
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 46, 473
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 403
BstDSI CCRYGG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 488
BstKTI GATC 3 cut(s) 144, 150, 451
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 3 cut(s) 141, 147, 448
BstMWI GCNNNNNNNGC 5 cut(s) 98, 267, 290, 453, 462
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstSFI CTRYAG 1 cut(s) 250
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 230, 267, 270
BstV2I GAAGAC 1 cut(s) 114
BsuRI GGCC 2 cut(s) 5, 407
BtgI CCRYGG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 398
CciI TCATGA 1 cut(s) 12
Cfr13I GGNCC 2 cut(s) 4, 121
CseI GACGC 1 cut(s) 454
CviAII CATG 1 cut(s) 13
CviJI RGCY 6 cut(s) 5, 19, 203, 221, 293, 407
CviKI_1 RGCY 6 cut(s) 5, 19, 203, 221, 293, 407
DpnI GATC 3 cut(s) 143, 149, 450
DpnII GATC 3 cut(s) 141, 147, 448
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 133
FaeI CATG 1 cut(s) 16
FaiI YATR 3 cut(s) 14, 314, 349
FatI CATG 1 cut(s) 12
FblI GTMKAC 2 cut(s) 180, 468
Fnu4HI GCNGC 3 cut(s) 219, 256, 259
Fsp4HI GCNGC 3 cut(s) 219, 256, 259
GluI GCNGC 3 cut(s) 219, 256, 259
HaeIII GGCC 2 cut(s) 5, 407
HgaI GACGC 1 cut(s) 454
Hin1II CATG 1 cut(s) 16
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HphI GGTGA 2 cut(s) 58, 440
Hpy166II GTNNAC 3 cut(s) 181, 400, 469
Hpy188I TCNGA 1 cut(s) 152
Hpy188III TCNNGA 4 cut(s) 13, 25, 104, 145
Hpy8I GTNNAC 3 cut(s) 181, 400, 469
Hpy99I CGWCG 1 cut(s) 332
HpyCH4III ACNGT 2 cut(s) 46, 473
HpyCH4IV ACGT 2 cut(s) 377, 402
HpyCH4V TGCA 4 cut(s) 35, 231, 300, 363
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 267, 290, 453, 462
HpySE526I ACGT 2 cut(s) 377, 402
Hsp92II CATG 1 cut(s) 16
Kzo9I GATC 3 cut(s) 141, 147, 448
LmnI GCTCC 1 cut(s) 275
LpnPI CCDG 3 cut(s) 120, 147, 258
Lsp1109I GCAGC 3 cut(s) 230, 267, 270
MaeII ACGT 2 cut(s) 377, 402
MaeIII GTNAC 6 cut(s) 25, 49, 64, 206, 434, 479
MalI GATC 3 cut(s) 143, 149, 450
MboI GATC 3 cut(s) 141, 147, 448
MboII GAAGA 1 cut(s) 119
MfeI CAATTG 1 cut(s) 30
MluCI AATT 1 cut(s) 30
MluI ACGCGT 1 cut(s) 486
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 221
MspR9I CCNGG 1 cut(s) 135
MunI CAATTG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 5 cut(s) 98, 267, 290, 453, 462
NdeII GATC 3 cut(s) 141, 147, 448
NlaIII CATG 1 cut(s) 16
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 3 cut(s) 25, 49, 64
PagI TCATGA 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PflFI GACNNNGTC 1 cut(s) 156
PkrI GCNGC 3 cut(s) 220, 257, 260
Ppu21I YACGTR 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspPI GGNCC 2 cut(s) 4, 121
PspPPI RGGWCCY 1 cut(s) 121
PsyI GACNNNGTC 1 cut(s) 156
PvuII CAGCTG 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 426
SalI GTCGAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 3 cut(s) 219, 256, 259
Sau3AI GATC 3 cut(s) 141, 147, 448
Sau96I GGNCC 2 cut(s) 4, 121
ScrFI CCNGG 1 cut(s) 135
SetI ASST 5 cut(s) 21, 126, 223, 380, 405
SfcI CTRYAG 1 cut(s) 250
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 426
Sse9I AATT 1 cut(s) 30
SsiI CCGC 3 cut(s) 90, 342, 426
StyD4I CCNGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 46, 473
TaiI ACGT 2 cut(s) 380, 405
TaqI TCGA 2 cut(s) 105, 180
TasI AATT 1 cut(s) 30
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 1 cut(s) 398
TseFI GTSAC 3 cut(s) 25, 49, 64
TseI GCWGC 3 cut(s) 218, 255, 258
Tsp45I GTSAC 3 cut(s) 25, 49, 64
TspDTI ATGAA 2 cut(s) 29, 258
TspRI CASTG 1 cut(s) 398
Tth111I GACNNNGTC 1 cut(s) 156
VpaK11BI GGWCC 1 cut(s) 121
XmiI GTMKAC 2 cut(s) 180, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.