MD11G1213000.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015225
Physical Location & Seq
Reverse (-)
31129480 .. 31129968
489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1213000.v1.1.491

Sequence Viewer

Length: 489 bp
ATGGAGGTCATCAAGATTATATTGATCATAGCAACAACCATGGCTTTGTCTATCACTCTCATGACCGTGAAAAGGTTCGGTTATGGAGTAGAAGAAGGAAAAACCCCTTTCACCGACTCGTGGAAAGAGCAACTGGAGGTCGATGAAAACACCTCGCTACTTCCTTCGAAGAGGGTGAGTCGTTTCCTAGCTGAAAAGGATCTGGTTGACAGAAACCCTAGAGCAGCTGACCATTGCCACAAAGACAATGAGGTATGTGCCTACACGCCCCCGGGCTATAAGAACTCAACGTGTTGCAATAACAAGTGCATAGACTTGTCCGAGGACAAGCACAACTGTGGTGCATGCAAGCAGAAGTGCAAGTACACTGAATCGTGTTGTAGGGGGGGTTGCGTGGACACAAATTATGACAAGAGGCATTGTGGCCAATGCAACAGTCCCTGCAAGTTGGGGCAGTTCTGTGTATATGGTCTTTGCAATTATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

163

Amino Acids

18.19

Weight (kDa)

7.84

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 77 - 162 1.4e-27 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 207
AcoI YGGCCR 1 cut(s) 424
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 72, 120
AflIII ACRYGT 1 cut(s) 290
AleI CACNNNNGTG 1 cut(s) 336
AluBI AGCT 2 cut(s) 191, 227
AluI AGCT 2 cut(s) 191, 227
AlwI GGATC 1 cut(s) 207
AlwNI CAGNNNCTG 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 271
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 74
AsuC2I CCSGG 2 cut(s) 272, 273
AsuHPI GGTGA 2 cut(s) 103, 187
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 271
BalI TGGCCA 1 cut(s) 426
BarI GAAGNNNNNNTAC 2 cut(s) 347, 379
BauI CACGAG 1 cut(s) 118
BbvI GCAGC 1 cut(s) 236
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 2 cut(s) 272, 273
BfaI CTAG 2 cut(s) 188, 219
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 272, 273
BmeT110I CYCGRG 1 cut(s) 271
BmrFI CCNGG 2 cut(s) 272, 273
BpmI CTGGAG 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 167
BpuMI CCSGG 2 cut(s) 272, 273
BsaJI CCNNGG 4 cut(s) 39, 270, 271, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 120
Bse1I ACTGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 232
BseDI CCNNGG 4 cut(s) 39, 270, 271, 321
BseLI CCNNNNNNNGG 2 cut(s) 72, 120
BseMI GCAATG 1 cut(s) 232
BseNI ACTGG 1 cut(s) 138
BseXI GCAGC 1 cut(s) 236
BshFI GGCC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 271
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 2 cut(s) 72, 120
BsmFI GGGAC 1 cut(s) 423
BsnI GGCC 1 cut(s) 426
BsoBI CYCGRG 1 cut(s) 271
Bsp119I TTCGAA 1 cut(s) 167
Bsp143I GATC 2 cut(s) 24, 199
Bsp19I CCATGG 1 cut(s) 39
BspANI GGCC 1 cut(s) 426
BspHI TCATGA 1 cut(s) 60
BspPI GGATC 1 cut(s) 207
BspT104I TTCGAA 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 232
BsrI ACTGG 1 cut(s) 138
BssECI CCNNGG 4 cut(s) 39, 270, 271, 321
BssMI GATC 2 cut(s) 24, 199
BssSI CACGAG 1 cut(s) 118
BssT1I CCWWGG 1 cut(s) 39
Bst2BI CACGAG 1 cut(s) 118
Bst4CI ACNGT 3 cut(s) 67, 338, 437
Bst6I CTCTTC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 346, 350
BstDSI CCRYGG 1 cut(s) 39
BstKTI GATC 2 cut(s) 27, 202
BstMBI GATC 2 cut(s) 24, 199
BstNSI RCATGY 1 cut(s) 348
BstSCI CCNGG 2 cut(s) 270, 271
BstV1I GCAGC 1 cut(s) 236
BstX2I RGATCY 1 cut(s) 199
BstYI RGATCY 1 cut(s) 199
BsuRI GGCC 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 366
Cac8I GCNNGC 2 cut(s) 346, 350
CaiI CAGNNNCTG 1 cut(s) 441
CciI TCATGA 1 cut(s) 60
Cfr9I CCCGGG 1 cut(s) 271
Csp6I GTAC 1 cut(s) 364
CviAII CATG 3 cut(s) 40, 61, 345
CviJI RGCY 5 cut(s) 44, 191, 227, 276, 426
CviKI_1 RGCY 5 cut(s) 44, 191, 227, 276, 426
CviQI GTAC 1 cut(s) 364
DpnI GATC 2 cut(s) 26, 201
DpnII GATC 2 cut(s) 24, 199
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 3 cut(s) 43, 64, 348
FaqI GGGAC 1 cut(s) 423
FatI CATG 3 cut(s) 39, 60, 344
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 188, 219
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 1 cut(s) 426
HapII CCGG 1 cut(s) 272
Hin1II CATG 3 cut(s) 43, 64, 348
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 3 cut(s) 116, 178, 371
HpaII CCGG 1 cut(s) 272
HphI GGTGA 2 cut(s) 103, 187
Hpy166II GTNNAC 3 cut(s) 208, 366, 397
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 13, 61
Hpy8I GTNNAC 3 cut(s) 208, 366, 397
HpyAV CCTTC 2 cut(s) 89, 174
HpyCH4III ACNGT 3 cut(s) 67, 338, 437
HpyCH4IV ACGT 1 cut(s) 290
HpyCH4V TGCA 8 cut(s) 297, 309, 344, 348, 360, 432, 444, 477
HpySE526I ACGT 1 cut(s) 290
Hsp92II CATG 3 cut(s) 43, 64, 348
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 2 cut(s) 24, 199
LpnPI CCDG 4 cut(s) 119, 188, 285, 454
Lsp1109I GCAGC 1 cut(s) 236
MaeI CTAG 2 cut(s) 188, 219
MaeII ACGT 1 cut(s) 290
MalI GATC 2 cut(s) 26, 201
MboI GATC 2 cut(s) 24, 199
MboII GAAGA 2 cut(s) 104, 181
MflI RGATCY 1 cut(s) 199
MlsI TGGCCA 1 cut(s) 426
MluCI AATT 2 cut(s) 403, 478
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 110, 187
MnlI CCTC 6 cut(s) 130, 163, 165, 244, 316, 408
Mox20I TGGCCA 1 cut(s) 426
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 426
MslI CAYNNNNRTG 3 cut(s) 59, 65, 336
Msp20I TGGCCA 1 cut(s) 426
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 272, 273
NciI CCSGG 2 cut(s) 272, 273
NcoI CCATGG 1 cut(s) 39
NdeII GATC 2 cut(s) 24, 199
NlaIII CATG 3 cut(s) 43, 64, 348
NspI RCATGY 1 cut(s) 348
NspV TTCGAA 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 336
PaeI GCATGC 1 cut(s) 348
PagI TCATGA 1 cut(s) 60
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 110, 186
PpsI GAGTC 2 cut(s) 110, 186
PstNI CAGNNNCTG 1 cut(s) 441
PsuI RGATCY 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 3 cut(s) 59, 65, 336
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 24, 199
SchI GAGTC 2 cut(s) 110, 187
ScrFI CCNGG 2 cut(s) 272, 273
SetI ASST 8 cut(s) 9, 77, 141, 155, 193, 229, 255, 293
SfuI TTCGAA 1 cut(s) 167
SmaI CCCGGG 1 cut(s) 273
SmiMI CAYNNNNRTG 3 cut(s) 59, 65, 336
SphI GCATGC 1 cut(s) 348
Sse9I AATT 2 cut(s) 403, 478
SspMI CTAG 2 cut(s) 188, 219
StyD4I CCNGG 2 cut(s) 270, 271
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 67, 338, 437
TaiI ACGT 1 cut(s) 293
TaqI TCGA 2 cut(s) 141, 167
TasI AATT 2 cut(s) 403, 478
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 1 cut(s) 371
TscAI CASTG 1 cut(s) 373
TseI GCWGC 1 cut(s) 224
TspDTI ATGAA 1 cut(s) 159
TspMI CCCGGG 1 cut(s) 271
TspRI CASTG 1 cut(s) 373
XceI RCATGY 1 cut(s) 348
XmaI CCCGGG 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 188, 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.