Prupe.4G218300_v2.0.a1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015225
Physical Location & Seq
Forward (+)
13724337 .. 13725270
934 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G218300.1

Sequence Viewer

Length: 492 bp
ATGAAGGTCATCAAGATTCTAGTGATCATAGCAACAACCATGGCTTTGTCTATCACTCTCACGACCGTGACAAGGGTTAGCCATCAAGAAGAAGAAGGCAAATCCCCTTTCACCGACCCATGGAAAGAGCAACCGTCGGCTGACGATGAAAACAAGTTGATAGTGCCCTCCAAGAGGGTGAGTCGCTTCCTTTCTGAAAAGGATCTGGTTTCAAGAAGCCTTAAAGCAGCTTCCCAGTGCCGCAATGACTATGACACATGCTCCAACGTGCTGCCGGGCCATCGGAACTTCACCTGCTGCAACAAGAAGTGCGTGGATTTGTCCGTAAACAAGGAAAACTGTGGTGCATGCAAGAATTGCTGCAAGTACACGCAGTCGTGTTGCAGAGGGCAGTGCGTGGACCTGAGTTTTGACAAGAGGCATTGTGGCCAGTGCGACCATCCCTGCAAGAATGGAACGTATTGCGTGTATGGTCTCTGTGATTATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

164

Amino Acids

18.23

Weight (kDa)

8.42

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 302
AciI CCGC 1 cut(s) 241
AclWI GGATC 1 cut(s) 210
AcoI YGGCCR 1 cut(s) 427
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 3 cut(s) 72, 120, 174
AgsI TTSAA 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 1 cut(s) 230
AluI AGCT 1 cut(s) 230
Alw26I GTCTC 1 cut(s) 479
AlwI GGATC 1 cut(s) 210
AoxI GGCC 2 cut(s) 277, 427
ApeKI GCWGC 4 cut(s) 227, 271, 297, 360
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspS9I GGNCC 2 cut(s) 277, 400
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 3 cut(s) 103, 190, 283
AvaII GGWCC 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 168
BalI TGGCCA 1 cut(s) 429
BbvI GCAGC 4 cut(s) 239, 258, 284, 347
BccI CCATC 3 cut(s) 90, 288, 447
BclI TGATCA 1 cut(s) 24
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 1 cut(s) 20
BfuAI ACCTGC 1 cut(s) 302
BisI GCNGC 5 cut(s) 228, 241, 272, 298, 361
BlsI GCNGC 5 cut(s) 229, 242, 273, 299, 362
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 2 cut(s) 277, 400
BmrFI CCNGG 1 cut(s) 276
BmrI ACTGGG 1 cut(s) 229
BmuI ACTGGG 1 cut(s) 229
BpuMI CCSGG 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 39, 119
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 120, 174
Bse1I ACTGG 2 cut(s) 235, 430
Bse3DI GCAATG 1 cut(s) 250
BseDI CCNNGG 2 cut(s) 39, 119
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 72, 120, 174
BseMI GCAATG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 2 cut(s) 235, 430
BseSI GKGCMC 1 cut(s) 168
BseXI GCAGC 4 cut(s) 239, 258, 284, 347
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 279, 429
BsiEI CGRYCG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 275
BslI CCNNNNNNNGG 3 cut(s) 72, 120, 174
BsmAI GTCTC 1 cut(s) 479
BsnI GGCC 2 cut(s) 279, 429
Bso31I GGTCTC 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 2 cut(s) 24, 202
Bsp19I CCATGG 2 cut(s) 39, 119
BspACI CCGC 1 cut(s) 241
BspANI GGCC 2 cut(s) 279, 429
BspCNI CTCAG 1 cut(s) 396
BspMI ACCTGC 1 cut(s) 302
BspPI GGATC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 2 cut(s) 235, 430
BssECI CCNNGG 2 cut(s) 39, 119
BssMI GATC 2 cut(s) 24, 202
BssT1I CCWWGG 2 cut(s) 39, 119
Bst4CI ACNGT 3 cut(s) 67, 135, 341
BstAPI GCANNNNNTGC 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 349
BstDEI CTNAG 1 cut(s) 404
BstDSI CCRYGG 2 cut(s) 39, 119
BstENI CCTNNNNNAGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 439
BstKTI GATC 2 cut(s) 27, 205
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 2 cut(s) 24, 202
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstNSI RCATGY 2 cut(s) 261, 351
BstSCI CCNGG 1 cut(s) 274
BstSLI GKGCMC 1 cut(s) 168
BstV1I GCAGC 4 cut(s) 239, 258, 284, 347
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 2 cut(s) 279, 429
BtgI CCRYGG 2 cut(s) 39, 119
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 3 cut(s) 242, 398, 437
BveI ACCTGC 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 277, 400
Csp6I GTAC 1 cut(s) 367
CviAII CATG 4 cut(s) 40, 120, 258, 348
CviJI RGCY 7 cut(s) 44, 81, 140, 219, 230, 279, 429
CviKI_1 RGCY 7 cut(s) 44, 81, 140, 219, 230, 279, 429
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 1 cut(s) 404
DpnI GATC 2 cut(s) 26, 204
DpnII GATC 2 cut(s) 24, 202
EaeI YGGCCR 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 39, 119
Eco31I GGTCTC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 400
EcoNI CCTNNNNNAGG 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 39, 119
ErhI CCWWGG 2 cut(s) 39, 119
FaeI CATG 4 cut(s) 43, 123, 261, 351
FaiI YATR 8 cut(s) 29, 41, 121, 252, 259, 349, 471, 486
FatI CATG 4 cut(s) 39, 119, 257, 347
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 5 cut(s) 228, 241, 272, 298, 361
FokI GGATG 1 cut(s) 426
Fsp4HI GCNGC 5 cut(s) 228, 241, 272, 298, 361
FspBI CTAG 1 cut(s) 20
GluI GCNGC 5 cut(s) 228, 241, 272, 298, 361
HaeIII GGCC 2 cut(s) 279, 429
HapII CCGG 1 cut(s) 275
Hin1II CATG 4 cut(s) 43, 123, 261, 351
HinfI GANTC 2 cut(s) 16, 181
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 103, 190, 283
Hpy166II GTNNAC 3 cut(s) 328, 369, 400
Hpy188I TCNGA 2 cut(s) 196, 285
Hpy188III TCNNGA 4 cut(s) 13, 61, 86, 213
Hpy8I GTNNAC 3 cut(s) 328, 369, 400
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 1 cut(s) 89
HpyCH4III ACNGT 3 cut(s) 67, 135, 341
HpyCH4IV ACGT 2 cut(s) 267, 458
HpyCH4V TGCA 6 cut(s) 300, 347, 351, 363, 384, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 1 cut(s) 404
HpySE526I ACGT 2 cut(s) 267, 458
Hsp92II CATG 4 cut(s) 43, 123, 261, 351
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 2 cut(s) 24, 202
LmnI GCTCC 1 cut(s) 266
LpnPI CCDG 7 cut(s) 191, 248, 288, 307, 416, 443, 457
Lsp1109I GCAGC 4 cut(s) 239, 258, 284, 347
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 267, 458
MaeIII GTNAC 1 cut(s) 67
MalI GATC 2 cut(s) 26, 204
MboI GATC 2 cut(s) 24, 202
MboII GAAGA 2 cut(s) 101, 104
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 168
MlsI TGGCCA 1 cut(s) 429
MluCI AATT 1 cut(s) 355
MluNI TGGCCA 1 cut(s) 429
MlyI GAGTC 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 4 cut(s) 168, 178, 380, 411
Mox20I TGGCCA 1 cut(s) 429
MscI TGGCCA 1 cut(s) 429
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 429
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 357
NciI CCSGG 1 cut(s) 276
NcoI CCATGG 2 cut(s) 39, 119
NdeII GATC 2 cut(s) 24, 202
NlaIII CATG 4 cut(s) 43, 123, 261, 351
NmuCI GTSAC 1 cut(s) 67
NspI RCATGY 2 cut(s) 261, 351
OliI CACNNNNGTG 1 cut(s) 65
PaeI GCATGC 1 cut(s) 351
PaqCI CACCTGC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 16
PkrI GCNGC 5 cut(s) 229, 242, 273, 299, 362
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PspPI GGNCC 2 cut(s) 277, 400
PsuI RGATCY 1 cut(s) 202
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 5 cut(s) 228, 241, 272, 298, 361
Sau3AI GATC 2 cut(s) 24, 202
Sau96I GGNCC 2 cut(s) 277, 400
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 1 cut(s) 276
SduI GDGCHC 1 cut(s) 168
SetI ASST 6 cut(s) 9, 232, 270, 296, 405, 461
SinI GGWCC 1 cut(s) 400
SmiMI CAYNNNNRTG 1 cut(s) 65
SphI GCATGC 1 cut(s) 351
Sse9I AATT 1 cut(s) 355
SsiI CCGC 1 cut(s) 241
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 274
StyI CCWWGG 2 cut(s) 39, 119
TaaI ACNGT 3 cut(s) 67, 135, 341
TaiI ACGT 2 cut(s) 270, 461
TasI AATT 1 cut(s) 355
TatI WGTACW 1 cut(s) 366
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 1 cut(s) 16
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 3 cut(s) 242, 398, 437
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 4 cut(s) 227, 271, 297, 360
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 2 cut(s) 17, 162
TspGWI ACGGA 1 cut(s) 313
TspRI CASTG 3 cut(s) 242, 398, 437
VpaK11BI GGWCC 1 cut(s) 400
XagI CCTNNNNNAGG 1 cut(s) 172
XceI RCATGY 2 cut(s) 261, 351
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.