Rroxscaffold_1G00039870

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015225
Physical Location & Seq
Reverse (-)
57546022 .. 57546450
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039870.1

Sequence Viewer

Length: 429 bp
ATGGCTTTGTCCATCACTCTCATGACCGTGAACAGGGTCGGTCGTGAAGACGAAGAGAAACCCTCTTTCGTGTCGGAAGAGCACCCCCTCTACGATGAGAAAAATCTGCTTCTGCCTTCTAAAAGGGTGAGTCGTTTCCTGGCTGAGACTAAAAACCCTAGAGCAGCCGACCATTGCAACAAGGACAACGAGATCTGCGCCTACTTGGCAGACAGCCACACGAACTCGACATGCTGCAACAACAAGTGCATGGACTTGTCCGAGGACAAGCACAACTGTGGCGCCTGCAAGAAGAAATGCAAGTACACTGAGACGTGCTGCAGAGGAGGCTGCGTTGACACGAATTTCGATAAGAGGCACTGCGGAGGCTGCAACCAGAGGTGCCAAAAAGGCCAGTATTGTGTGTACGGCCTGTGTAACTATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

142

Amino Acids

16.04

Weight (kDa)

7.85

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 57 - 142 1.2e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 281, 381
AciI CCGC 1 cut(s) 363
AcsI RAATTY 1 cut(s) 343
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 305, 407
AfiI CCNNNNNNNGG 1 cut(s) 33
AjiI CACGTC 1 cut(s) 315
AjnI CCWGG 1 cut(s) 138
AleI CACNNNNGTG 1 cut(s) 276
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 140, 305
AoxI GGCC 2 cut(s) 391, 409
ApeKI GCWGC 5 cut(s) 164, 234, 318, 330, 369
ApoI RAATTY 1 cut(s) 343
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AspLEI GCGC 2 cut(s) 200, 284
AsuHPI GGTGA 1 cut(s) 139
BanI GGYRCC 2 cut(s) 281, 381
BbsI GAAGAC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 84
BbvI GCAGC 5 cut(s) 176, 221, 305, 317, 356
BccI CCATC 1 cut(s) 20
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 2 cut(s) 140, 305
BfaI CTAG 1 cut(s) 159
BfmI CTRYAG 1 cut(s) 319
BfoI RGCGCY 1 cut(s) 285
BglI GCCNNNNNGGC 2 cut(s) 206, 390
BglII AGATCT 1 cut(s) 192
BisI GCNGC 5 cut(s) 165, 235, 319, 331, 370
BlsI GCNGC 5 cut(s) 166, 236, 320, 332, 371
Bme1390I CCNGG 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 315
BmiI GGNNCC 2 cut(s) 283, 383
BmrFI CCNGG 1 cut(s) 140
BpiI GAAGAC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 47, 79
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 172
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 1 cut(s) 261
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 172
BseMII CTCAG 2 cut(s) 135, 300
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 339
BseXI GCAGC 5 cut(s) 176, 221, 305, 317, 356
Bsh1285I CGRYCG 1 cut(s) 43
BshFI GGCC 2 cut(s) 393, 411
BshNI GGYRCC 2 cut(s) 281, 381
BsiEI CGRYCG 1 cut(s) 43
BsiHKAI GWGCWC 1 cut(s) 84
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 2 cut(s) 140, 305
BsmBI CGTCTC 1 cut(s) 305
BsnI GGCC 2 cut(s) 393, 411
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 1 cut(s) 363
BspANI GGCC 2 cut(s) 393, 411
BspCNI CTCAG 2 cut(s) 136, 301
BspHI TCATGA 1 cut(s) 21
BspLI GGNNCC 2 cut(s) 283, 383
BspMAI CTGCAG 1 cut(s) 323
BspQI GCTCTTC 1 cut(s) 72
BspT107I GGYRCC 2 cut(s) 281, 381
BsrDI GCAATG 1 cut(s) 172
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 192
BssNI GRCGYC 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 28, 278
Bst6I CTCTTC 2 cut(s) 48, 72
BstACI GRCGYC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 144, 309
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 2 cut(s) 200, 284
BstKTI GATC 1 cut(s) 195
BstMAI GTCTC 2 cut(s) 140, 305
BstMBI GATC 1 cut(s) 192
BstMCI CGRYCG 1 cut(s) 43
BstMWI GCNNNNNNNGC 4 cut(s) 206, 327, 369, 390
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 319
BstV1I GCAGC 5 cut(s) 176, 221, 305, 317, 356
BstV2I GAAGAC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 2 cut(s) 393, 411
BtrI CACGTC 1 cut(s) 315
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 2 cut(s) 306, 358
Cac8I GCNNGC 1 cut(s) 286
CciI TCATGA 1 cut(s) 21
CfoI GCGC 2 cut(s) 200, 284
Csp6I GTAC 2 cut(s) 304, 406
CviAII CATG 3 cut(s) 22, 231, 250
CviJI RGCY 8 cut(s) 5, 143, 167, 216, 330, 369, 393, 411
CviKI_1 RGCY 8 cut(s) 5, 143, 167, 216, 330, 369, 393, 411
CviQI GTAC 2 cut(s) 304, 406
DdeI CTNAG 2 cut(s) 144, 309
DinI GGCGCC 1 cut(s) 283
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 48, 72
EarI CTCTTC 2 cut(s) 48, 72
EcoRII CCWGG 1 cut(s) 138
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 3 cut(s) 25, 234, 253
FaiI YATR 4 cut(s) 23, 232, 251, 423
FatI CATG 3 cut(s) 21, 230, 249
Fnu4HI GCNGC 5 cut(s) 165, 235, 319, 331, 370
Fsp4HI GCNGC 5 cut(s) 165, 235, 319, 331, 370
FspBI CTAG 1 cut(s) 159
GlaI GCGC 2 cut(s) 199, 283
GluI GCNGC 5 cut(s) 165, 235, 319, 331, 370
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 2 cut(s) 393, 411
HhaI GCGC 2 cut(s) 200, 284
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 3 cut(s) 25, 234, 253
Hin6I GCGC 2 cut(s) 198, 282
HinP1I GCGC 2 cut(s) 198, 282
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 1 cut(s) 130
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 4 cut(s) 31, 306, 337, 406
Hpy188I TCNGA 2 cut(s) 76, 262
Hpy188III TCNNGA 2 cut(s) 22, 44
Hpy8I GTNNAC 4 cut(s) 31, 306, 337, 406
HpyAV CCTTC 1 cut(s) 126
HpyCH4III ACNGT 2 cut(s) 28, 278
HpyCH4IV ACGT 1 cut(s) 314
HpyCH4V TGCA 7 cut(s) 177, 237, 249, 288, 300, 321, 372
HpyF10VI GCNNNNNNNGC 4 cut(s) 206, 327, 369, 390
HpyF3I CTNAG 2 cut(s) 144, 309
HpySE526I ACGT 1 cut(s) 314
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 3 cut(s) 25, 234, 253
HspAI GCGC 2 cut(s) 198, 282
KasI GGCGCC 1 cut(s) 281
Kzo9I GATC 1 cut(s) 192
LguI GCTCTTC 1 cut(s) 72
LpnPI CCDG 7 cut(s) 19, 125, 152, 298, 389, 407, 425
Lsp1109I GCAGC 5 cut(s) 176, 221, 305, 317, 356
MaeI CTAG 1 cut(s) 159
MaeII ACGT 1 cut(s) 314
MaeIII GTNAC 1 cut(s) 416
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MboII GAAGA 4 cut(s) 59, 65, 89, 304
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 1 cut(s) 343
Mly113I GGCGCC 1 cut(s) 282
MlyI GAGTC 1 cut(s) 139
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 8 cut(s) 73, 98, 256, 317, 320, 348, 359, 372
MslI CAYNNNNRTG 3 cut(s) 20, 26, 276
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 4 cut(s) 206, 327, 369, 390
NarI GGCGCC 1 cut(s) 282
NdeII GATC 1 cut(s) 192
NlaIII CATG 3 cut(s) 25, 234, 253
NlaIV GGNNCC 2 cut(s) 283, 383
NspI RCATGY 1 cut(s) 234
OliI CACNNNNGTG 1 cut(s) 276
PagI TCATGA 1 cut(s) 21
PciSI GCTCTTC 1 cut(s) 72
PkrI GCNGC 5 cut(s) 166, 236, 320, 332, 371
PleI GAGTC 1 cut(s) 138
PluTI GGCGCC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 283, 383
PstI CTGCAG 1 cut(s) 323
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 2 cut(s) 305, 407
RsaNI GTAC 2 cut(s) 304, 406
RseI CAYNNNNRTG 3 cut(s) 20, 26, 276
SapI GCTCTTC 1 cut(s) 72
SatI GCNGC 5 cut(s) 165, 235, 319, 331, 370
Sau3AI GATC 1 cut(s) 192
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 1 cut(s) 140
SduI GDGCHC 1 cut(s) 84
SetI ASST 2 cut(s) 317, 383
SfcI CTRYAG 1 cut(s) 319
SfoI GGCGCC 1 cut(s) 283
SmiMI CAYNNNNRTG 3 cut(s) 20, 26, 276
Sse9I AATT 1 cut(s) 343
SsiI CCGC 1 cut(s) 363
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 28, 278
TaiI ACGT 1 cut(s) 317
TaqI TCGA 2 cut(s) 227, 348
TaqII GACCGA 1 cut(s) 29
TasI AATT 1 cut(s) 343
TatI WGTACW 1 cut(s) 303
TscAI CASTG 2 cut(s) 313, 365
TseI GCWGC 5 cut(s) 164, 234, 318, 330, 369
TspRI CASTG 2 cut(s) 313, 365
XapI RAATTY 1 cut(s) 343
XceI RCATGY 1 cut(s) 234
XspI CTAG 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.