Rh5DG286500

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015225
Physical Location & Seq
Forward (+)
35588424 .. 35588924
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG286500.1

Sequence Viewer

Length: 468 bp
ATGGAGCTCATCAAGATTATGCTGATCATAGCAACAACCATGGCTTTGTCCATCACTCTCATGACCGTGAACAGGGTCGGTAGTGAAGAAGAAGAGAAACCCTCTTTCGTGTCGGAAGAGCACCCCCTCTACGACGAGAAAAATCTGCTTCTGCCTTCGAAAAGGGTGAGTCGTTTCCTGGCTGAGACTAAAAACCCTAGAGCAGCAGACCATTGCAACAAGGACAACGAGATCTGCGCCTACTTGGCAGACAGCCACACGAACTCGACATGCTGCAACAACAAGTGCATGGACTTGTCCGAGGACAAGCACAACTGCGGCTCCTGCAAGAAGAAATGCAAGTACACTGAGACATGCTGCAGAGGAGGCTGCGTTGACACGAATTTCGATAAGAGGCACTGCGGAGGCTGCAACCAGCGGTGCCAAAAAGGCCAGTATTGTGTGTACGGCCTGTGTAACTATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

155

Amino Acids

17.47

Weight (kDa)

7.42

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 70 - 155 1.8e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 420
AciI CCGC 3 cut(s) 318, 402, 418
AcsI RAATTY 1 cut(s) 382
AfaI GTAC 2 cut(s) 344, 446
AfiI CCNNNNNNNGG 1 cut(s) 72
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 1 cut(s) 7
AluI AGCT 1 cut(s) 7
Alw21I GWGCWC 2 cut(s) 9, 123
Alw26I GTCTC 2 cut(s) 179, 344
AoxI GGCC 2 cut(s) 430, 448
ApeKI GCWGC 5 cut(s) 203, 273, 357, 369, 408
ApoI RAATTY 1 cut(s) 382
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspLEI GCGC 1 cut(s) 239
AsuHPI GGTGA 1 cut(s) 178
AsuII TTCGAA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 420
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 9, 123
BbvI GCAGC 5 cut(s) 215, 260, 344, 356, 395
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 1 cut(s) 179
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 179, 344
BfaI CTAG 1 cut(s) 198
BfmI CTRYAG 1 cut(s) 358
BglI GCCNNNNNGGC 2 cut(s) 245, 429
BglII AGATCT 1 cut(s) 231
BisI GCNGC 6 cut(s) 204, 274, 319, 358, 370, 409
BlsI GCNGC 6 cut(s) 205, 275, 320, 359, 371, 410
Bme1390I CCNGG 1 cut(s) 179
BmiI GGNNCC 2 cut(s) 322, 422
BmrFI CCNGG 1 cut(s) 179
BplI GAGNNNNNCTC 2 cut(s) 86, 118
Bpu14I TTCGAA 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 39, 300
BsaXI ACNNNNNCTCC 4 cut(s) 305, 335, 357, 387
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 39, 300
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 2 cut(s) 174, 339
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 5 cut(s) 215, 260, 344, 356, 395
BshFI GGCC 2 cut(s) 432, 450
BshNI GGYRCC 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 9, 123
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 2 cut(s) 179, 344
BsnI GGCC 2 cut(s) 432, 450
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 9, 123
Bsp143I GATC 2 cut(s) 24, 231
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 3 cut(s) 318, 402, 418
BspANI GGCC 2 cut(s) 432, 450
BspCNI CTCAG 2 cut(s) 175, 340
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 322, 422
BspMAI CTGCAG 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 420
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 39, 300
BssMI GATC 2 cut(s) 24, 231
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 2 cut(s) 87, 111
BstBI TTCGAA 1 cut(s) 158
BstDEI CTNAG 2 cut(s) 183, 348
BstDSI CCRYGG 1 cut(s) 39
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 2 cut(s) 27, 234
BstMAI GTCTC 2 cut(s) 179, 344
BstMBI GATC 2 cut(s) 24, 231
BstMWI GCNNNNNNNGC 5 cut(s) 245, 324, 366, 408, 429
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 2 cut(s) 273, 357
BstSCI CCNGG 1 cut(s) 177
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 5 cut(s) 215, 260, 344, 356, 395
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 2 cut(s) 432, 450
BtgI CCRYGG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 2 cut(s) 345, 397
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 343, 445
CviAII CATG 5 cut(s) 40, 61, 270, 289, 354
CviJI RGCY 9 cut(s) 7, 44, 182, 255, 321, 369, 408, 432, 450
CviKI_1 RGCY 9 cut(s) 7, 44, 182, 255, 321, 369, 408, 432, 450
CviQI GTAC 2 cut(s) 343, 445
DdeI CTNAG 2 cut(s) 183, 348
DpnI GATC 2 cut(s) 26, 233
DpnII GATC 2 cut(s) 24, 231
Eam1104I CTCTTC 2 cut(s) 87, 111
EarI CTCTTC 2 cut(s) 87, 111
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 5 cut(s) 43, 64, 273, 292, 357
FaiI YATR 8 cut(s) 20, 29, 41, 62, 271, 290, 355, 462
FatI CATG 5 cut(s) 39, 60, 269, 288, 353
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 6 cut(s) 204, 274, 319, 358, 370, 409
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 6 cut(s) 204, 274, 319, 358, 370, 409
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 238
GluI GCNGC 6 cut(s) 204, 274, 319, 358, 370, 409
HaeIII GGCC 2 cut(s) 432, 450
HhaI GCGC 1 cut(s) 239
Hin1II CATG 5 cut(s) 43, 64, 273, 292, 357
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 1 cut(s) 169
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 70, 345, 376, 445
Hpy188I TCNGA 2 cut(s) 115, 301
Hpy188III TCNNGA 2 cut(s) 13, 61
Hpy8I GTNNAC 4 cut(s) 70, 345, 376, 445
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 7 cut(s) 216, 276, 288, 327, 339, 360, 411
HpyF10VI GCNNNNNNNGC 5 cut(s) 245, 324, 366, 408, 429
HpyF3I CTNAG 2 cut(s) 183, 348
Hsp92II CATG 5 cut(s) 43, 64, 273, 292, 357
HspAI GCGC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 2 cut(s) 24, 231
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 2 cut(s) 4, 326
LpnPI CCDG 7 cut(s) 58, 164, 191, 337, 428, 446, 464
Lsp1109I GCAGC 5 cut(s) 215, 260, 344, 356, 395
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 455
MalI GATC 2 cut(s) 26, 233
MboI GATC 2 cut(s) 24, 231
MboII GAAGA 5 cut(s) 98, 101, 104, 128, 343
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 9, 123
MluCI AATT 1 cut(s) 382
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 7 cut(s) 112, 137, 295, 356, 359, 387, 398
MslI CAYNNNNRTG 2 cut(s) 59, 65
MspA1I CMGCKG 1 cut(s) 418
MspR9I CCNGG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 5 cut(s) 245, 324, 366, 408, 429
NcoI CCATGG 1 cut(s) 39
NdeII GATC 2 cut(s) 24, 231
NlaIII CATG 5 cut(s) 43, 64, 273, 292, 357
NlaIV GGNNCC 2 cut(s) 322, 422
NspI RCATGY 2 cut(s) 273, 357
NspV TTCGAA 1 cut(s) 158
PagI TCATGA 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 111
PkrI GCNGC 6 cut(s) 205, 275, 320, 359, 371, 410
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 322, 422
PstI CTGCAG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 2 cut(s) 344, 446
RsaNI GTAC 2 cut(s) 343, 445
RseI CAYNNNNRTG 2 cut(s) 59, 65
SacI GAGCTC 1 cut(s) 9
SapI GCTCTTC 1 cut(s) 111
SatI GCNGC 6 cut(s) 204, 274, 319, 358, 370, 409
Sau3AI GATC 2 cut(s) 24, 231
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 9, 123
SetI ASST 1 cut(s) 9
SfcI CTRYAG 1 cut(s) 358
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 59, 65
Sse9I AATT 1 cut(s) 382
SsiI CCGC 3 cut(s) 318, 402, 418
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 3 cut(s) 158, 266, 387
TasI AATT 1 cut(s) 382
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 321
TscAI CASTG 2 cut(s) 352, 404
TseI GCWGC 5 cut(s) 203, 273, 357, 369, 408
TspRI CASTG 2 cut(s) 352, 404
XapI RAATTY 1 cut(s) 382
XceI RCATGY 2 cut(s) 273, 357
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.