FvH4_3g23190

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015225
Physical Location & Seq
Forward (+)
16269064 .. 16270148
1085 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23190.t1

Sequence Viewer

Length: 468 bp
ATGGAGCTCATCAAGATTATGCTTATCATAGCAACAACCATGGCTTTGTCCATCACTCTCATGACTGTGAACAGGGGCGGTCGTGAAGAAGAAGATAAACCCTCGTTTGTGTCGGAAGAGCACCCACTCTACGACCAAAACAATCTGCTTCTGCCTTCGAAAAGGGTGAGTCGTTTCCTGGCTGAGACTAAGAACCCTAGAGCAGCTGACCATTGCAACAAGGACAACGAGATCTGCGCCTACTTGGCAGAGAGCAACACCAACTCGACTTGCTGCAACAACAAGTGCATGGACTTGTCCGAGGACAAGCACAACTGTGGCGCCTGCAAGAAGAAATGCAAGTACACTGACACTTGTTGCAGAGGAGGCTGCGTCGACACCAACTTCGACAAGAGGCACTGTGGAGGCTGCAACAACCGCTGTGAAAAAGGCCAGTATTGTGTGTACGGCATGTGTAACTATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

156

Amino Acids

17.43

Weight (kDa)

7.4

Isoelectric Point (pI)

33.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 70 - 155 1.4e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 375
AciI CCGC 2 cut(s) 78, 418
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 2 cut(s) 344, 446
AjnI CCWGG 1 cut(s) 177
AleI CACNNNNGTG 1 cut(s) 315
AluBI AGCT 2 cut(s) 7, 206
AluI AGCT 2 cut(s) 7, 206
Alw21I GWGCWC 2 cut(s) 9, 123
Alw26I GTCTC 1 cut(s) 179
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 4 cut(s) 203, 273, 369, 408
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspLEI GCGC 2 cut(s) 239, 323
AsuHPI GGTGA 1 cut(s) 178
AsuII TTCGAA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 2 cut(s) 9, 123
BbvI GCAGC 4 cut(s) 215, 260, 356, 395
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 463
BciT130I CCWGG 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 324
BglI GCCNNNNNGGC 1 cut(s) 245
BglII AGATCT 1 cut(s) 231
BisI GCNGC 4 cut(s) 204, 274, 370, 409
BlsI GCNGC 4 cut(s) 205, 275, 371, 410
Bme1390I CCNGG 1 cut(s) 179
BmiI GGNNCC 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 39, 300
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 211
BseBI CCWGG 1 cut(s) 179
BseDI CCNNGG 2 cut(s) 39, 300
BseMI GCAATG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 4 cut(s) 215, 260, 356, 395
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 1 cut(s) 432
BshNI GGYRCC 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 2 cut(s) 9, 123
BsmAI GTCTC 1 cut(s) 179
BsnI GGCC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 9, 123
Bsp143I GATC 1 cut(s) 231
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 2 cut(s) 78, 418
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 1 cut(s) 175
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 322
BspQI GCTCTTC 1 cut(s) 111
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 1 cut(s) 320
BsrDI GCAATG 1 cut(s) 211
BsrI ACTGG 1 cut(s) 433
BssECI CCNNGG 2 cut(s) 39, 300
BssMI GATC 1 cut(s) 231
BssNI GRCGYC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 3 cut(s) 67, 317, 401
Bst6I CTCTTC 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 2 cut(s) 183, 189
BstDSI CCRYGG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 324
BstHHI GCGC 2 cut(s) 239, 323
BstKTI GATC 1 cut(s) 234
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 231
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 245, 366, 417
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 215, 260, 356, 395
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 1 cut(s) 432
BtgI CCRYGG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 345, 397
Cac8I GCNNGC 1 cut(s) 325
CciI TCATGA 1 cut(s) 60
CfoI GCGC 2 cut(s) 239, 323
CseI GACGC 1 cut(s) 361
Csp6I GTAC 2 cut(s) 343, 445
CviAII CATG 4 cut(s) 40, 61, 289, 451
CviJI RGCY 7 cut(s) 7, 44, 182, 206, 369, 408, 432
CviKI_1 RGCY 7 cut(s) 7, 44, 182, 206, 369, 408, 432
CviQI GTAC 2 cut(s) 343, 445
DdeI CTNAG 2 cut(s) 183, 189
DinI GGCGCC 1 cut(s) 322
DpnI GATC 1 cut(s) 233
DpnII GATC 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 9
EgeI GGCGCC 1 cut(s) 322
EheI GGCGCC 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 4 cut(s) 43, 64, 292, 454
FaiI YATR 7 cut(s) 20, 29, 41, 62, 290, 452, 462
FatI CATG 4 cut(s) 39, 60, 288, 450
FblI GTMKAC 1 cut(s) 375
Fnu4HI GCNGC 4 cut(s) 204, 274, 370, 409
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 4 cut(s) 204, 274, 370, 409
FspBI CTAG 1 cut(s) 198
GlaI GCGC 2 cut(s) 238, 322
GluI GCNGC 4 cut(s) 204, 274, 370, 409
HaeII RGCGCY 1 cut(s) 324
HaeIII GGCC 1 cut(s) 432
HgaI GACGC 1 cut(s) 361
HhaI GCGC 2 cut(s) 239, 323
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 4 cut(s) 43, 64, 292, 454
Hin6I GCGC 2 cut(s) 237, 321
HinP1I GCGC 2 cut(s) 237, 321
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 1 cut(s) 169
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 4 cut(s) 70, 345, 376, 445
Hpy188I TCNGA 2 cut(s) 115, 301
Hpy188III TCNNGA 3 cut(s) 13, 61, 83
Hpy8I GTNNAC 4 cut(s) 70, 345, 376, 445
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 3 cut(s) 67, 317, 401
HpyCH4V TGCA 7 cut(s) 216, 276, 288, 327, 339, 360, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 366, 417
HpyF3I CTNAG 2 cut(s) 183, 189
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 4 cut(s) 43, 64, 292, 454
HspAI GCGC 2 cut(s) 237, 321
KasI GGCGCC 1 cut(s) 320
Kzo9I GATC 1 cut(s) 231
LguI GCTCTTC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 58, 164, 191, 337, 446
Lsp1109I GCAGC 4 cut(s) 215, 260, 356, 395
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 455
MalI GATC 1 cut(s) 233
MboI GATC 1 cut(s) 231
MboII GAAGA 5 cut(s) 98, 101, 104, 128, 343
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 2 cut(s) 9, 123
Mly113I GGCGCC 1 cut(s) 321
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 6 cut(s) 112, 295, 356, 359, 387, 398
MslI CAYNNNNRTG 3 cut(s) 59, 65, 315
MspA1I CMGCKG 2 cut(s) 206, 420
MspR9I CCNGG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 3 cut(s) 245, 366, 417
NarI GGCGCC 1 cut(s) 321
NcoI CCATGG 1 cut(s) 39
NdeII GATC 1 cut(s) 231
NlaIII CATG 4 cut(s) 43, 64, 292, 454
NlaIV GGNNCC 1 cut(s) 322
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 158
OliI CACNNNNGTG 1 cut(s) 315
PagI TCATGA 1 cut(s) 60
PciSI GCTCTTC 1 cut(s) 111
PkrI GCNGC 4 cut(s) 205, 275, 371, 410
PleI GAGTC 1 cut(s) 177
PluTI GGCGCC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 9
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 1 cut(s) 322
PsuI RGATCY 1 cut(s) 231
PvuII CAGCTG 1 cut(s) 206
RsaI GTAC 2 cut(s) 344, 446
RsaNI GTAC 2 cut(s) 343, 445
RseI CAYNNNNRTG 3 cut(s) 59, 65, 315
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 374
SapI GCTCTTC 1 cut(s) 111
SatI GCNGC 4 cut(s) 204, 274, 370, 409
Sau3AI GATC 1 cut(s) 231
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 179
SduI GDGCHC 2 cut(s) 9, 123
SetI ASST 2 cut(s) 9, 208
SfoI GGCGCC 1 cut(s) 322
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 3 cut(s) 59, 65, 315
SsiI CCGC 2 cut(s) 78, 418
SspDI GGCGCC 1 cut(s) 320
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 177
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 67, 317, 401
TaqI TCGA 4 cut(s) 158, 266, 375, 387
TatI WGTACW 1 cut(s) 342
TscAI CASTG 2 cut(s) 352, 404
TseI GCWGC 4 cut(s) 203, 273, 369, 408
TspRI CASTG 2 cut(s) 352, 404
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 1 cut(s) 375
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.