MD03G1197000.v1.1

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015225
Physical Location & Seq
Reverse (-)
26930592 .. 26931047
456 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1197000.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGCTTTGTCTATCACTCTCATGACCGTGAAAAGGGTCGGTTATGGAGAAGCAGAACAAGAAGGAAAAACCCCTTTCACCGACTCGTGGAAAGAGCAACCGGAGGTCGATGAAAACACCTTGCTACTACCTTCCAAGAGGGTGAGTCGTTTCCTAGCTGAAAATGATCTGGTTGACAGAAACCCTAGAGCAGCTGACCATTGCCACAAAGACAATGAGGTATGTGCCTACACGCCGCCAGGATATAAGAACTCGACGTGTTGCAACAACAAGTGCCTGGACTTGTCCGAGGACAAGAACAACTGTGGTGCATGCAGGAAGAAGTGCAAGTACACTGAATCGTGCTGTAGAGGAGAGTGTGTGAACACGAATTATGACAAGAGGCATTGTGGCCAGTGCAACAGTCCCTGCAAGTTTGGGCAGTACTGTGTATATGGTCTTTGCAATTATGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

152

Amino Acids

17.05

Weight (kDa)

7.38

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Stig1 PF04885 66 - 151 6.5e-28 Stigma-specific STIG1-like protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 236
AcoI YGGCCR 1 cut(s) 391
AfaI GTAC 2 cut(s) 332, 425
AfiI CCNNNNNNNGG 2 cut(s) 33, 87
AflIII ACRYGT 1 cut(s) 257
AjiI CACGTC 1 cut(s) 258
AjnI CCWGG 2 cut(s) 238, 276
AluBI AGCT 2 cut(s) 158, 194
AluI AGCT 2 cut(s) 158, 194
AlwNI CAGNNNCTG 1 cut(s) 408
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 191
AsuHPI GGTGA 2 cut(s) 70, 154
BalI TGGCCA 1 cut(s) 393
BarI GAAGNNNNNNTAC 2 cut(s) 314, 346
BauI CACGAG 1 cut(s) 85
BbvI GCAGC 1 cut(s) 203
BciT130I CCWGG 2 cut(s) 240, 278
BfaI CTAG 2 cut(s) 155, 186
BfmI CTRYAG 1 cut(s) 346
BisI GCNGC 2 cut(s) 192, 236
BlsI GCNGC 2 cut(s) 193, 237
BmcAI AGTACT 1 cut(s) 425
Bme1390I CCNGG 2 cut(s) 240, 278
BmgBI CACGTC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 240, 278
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 87
Bse1I ACTGG 1 cut(s) 394
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 240, 278
BseDI CCNNGG 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 33, 87
BseMI GCAATG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 203
BshFI GGCC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 101
BslFI GGGAC 1 cut(s) 390
BslI CCNNNNNNNGG 2 cut(s) 33, 87
BsmFI GGGAC 1 cut(s) 390
BsnI GGCC 1 cut(s) 393
Bsp143I GATC 1 cut(s) 166
BspACI CCGC 1 cut(s) 236
BspANI GGCC 1 cut(s) 393
BspHI TCATGA 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 1 cut(s) 166
BssSI CACGAG 1 cut(s) 85
Bst2BI CACGAG 1 cut(s) 85
Bst2UI CCWGG 2 cut(s) 240, 278
Bst4CI ACNGT 4 cut(s) 28, 305, 404, 428
BstC8I GCNNGC 1 cut(s) 313
BstKTI GATC 1 cut(s) 169
BstMBI GATC 1 cut(s) 166
BstNI CCWGG 2 cut(s) 240, 278
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 2 cut(s) 238, 276
BstSFI CTRYAG 1 cut(s) 346
BstV1I GCAGC 1 cut(s) 203
BsuRI GGCC 1 cut(s) 393
BtrI CACGTC 1 cut(s) 258
BtsIMutI CAGTG 2 cut(s) 333, 401
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 408
CciI TCATGA 1 cut(s) 21
Csp6I GTAC 2 cut(s) 331, 424
CviAII CATG 2 cut(s) 22, 312
CviJI RGCY 4 cut(s) 5, 158, 194, 393
CviKI_1 RGCY 4 cut(s) 5, 158, 194, 393
CviQI GTAC 2 cut(s) 331, 424
DpnI GATC 1 cut(s) 168
DpnII GATC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 391
EcoRII CCWGG 2 cut(s) 238, 276
FaeI CATG 2 cut(s) 25, 315
FaiI YATR 9 cut(s) 23, 45, 223, 246, 313, 375, 433, 435, 450
FaqI GGGAC 1 cut(s) 390
FatI CATG 2 cut(s) 21, 311
Fnu4HI GCNGC 2 cut(s) 192, 236
Fsp4HI GCNGC 2 cut(s) 192, 236
FspBI CTAG 2 cut(s) 155, 186
GluI GCNGC 2 cut(s) 192, 236
HaeIII GGCC 1 cut(s) 393
HapII CCGG 1 cut(s) 101
Hin1II CATG 2 cut(s) 25, 315
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 3 cut(s) 83, 145, 338
HpaII CCGG 1 cut(s) 101
HphI GGTGA 2 cut(s) 70, 154
Hpy166II GTNNAC 3 cut(s) 175, 333, 364
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 1 cut(s) 22
Hpy8I GTNNAC 3 cut(s) 175, 333, 364
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 2 cut(s) 56, 141
HpyCH4III ACNGT 4 cut(s) 28, 305, 404, 428
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 7 cut(s) 264, 311, 315, 327, 399, 411, 444
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 2 cut(s) 25, 315
Kzo9I GATC 1 cut(s) 166
LpnPI CCDG 9 cut(s) 114, 155, 225, 252, 263, 290, 301, 407, 421
Lsp1109I GCAGC 1 cut(s) 203
MaeI CTAG 2 cut(s) 155, 186
MaeII ACGT 1 cut(s) 257
MalI GATC 1 cut(s) 168
MboI GATC 1 cut(s) 166
MboII GAAGA 1 cut(s) 331
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 2 cut(s) 370, 445
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 2 cut(s) 77, 154
MnlI CCTC 6 cut(s) 97, 132, 211, 283, 344, 375
Mox20I TGGCCA 1 cut(s) 393
MscI TGGCCA 1 cut(s) 393
MslI CAYNNNNRTG 2 cut(s) 20, 26
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 240, 278
MvaI CCWGG 2 cut(s) 240, 278
NdeII GATC 1 cut(s) 166
NlaIII CATG 2 cut(s) 25, 315
NspI RCATGY 1 cut(s) 315
PaeI GCATGC 1 cut(s) 315
PagI TCATGA 1 cut(s) 21
PfeI GAWTC 1 cut(s) 338
PkrI GCNGC 2 cut(s) 193, 237
PleI GAGTC 2 cut(s) 77, 153
PpsI GAGTC 2 cut(s) 77, 153
Psp6I CCWGG 2 cut(s) 238, 276
PspGI CCWGG 2 cut(s) 238, 276
PstNI CAGNNNCTG 1 cut(s) 408
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 2 cut(s) 332, 425
RsaNI GTAC 2 cut(s) 331, 424
RseI CAYNNNNRTG 2 cut(s) 20, 26
SatI GCNGC 2 cut(s) 192, 236
Sau3AI GATC 1 cut(s) 166
ScaI AGTACT 1 cut(s) 425
SchI GAGTC 2 cut(s) 77, 154
ScrFI CCNGG 2 cut(s) 240, 278
SetI ASST 7 cut(s) 108, 122, 133, 160, 196, 222, 260
SfcI CTRYAG 1 cut(s) 346
SmiMI CAYNNNNRTG 2 cut(s) 20, 26
SphI GCATGC 1 cut(s) 315
Sse9I AATT 2 cut(s) 370, 445
SsiI CCGC 1 cut(s) 236
SspMI CTAG 2 cut(s) 155, 186
StyD4I CCNGG 2 cut(s) 238, 276
TaaI ACNGT 4 cut(s) 28, 305, 404, 428
TaiI ACGT 1 cut(s) 260
TaqI TCGA 2 cut(s) 108, 254
TasI AATT 2 cut(s) 370, 445
TatI WGTACW 2 cut(s) 330, 423
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 1 cut(s) 338
TscAI CASTG 2 cut(s) 340, 401
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 126
TspRI CASTG 2 cut(s) 340, 401
XceI RCATGY 1 cut(s) 315
XspI CTAG 2 cut(s) 155, 186
ZrmI AGTACT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.