RLG00000034006

Stigma-specific protein, Stig1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015225
Physical Location & Seq
Forward (+)
35263085 .. 35263666
582 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034006

Sequence Viewer

Length: 429 bp
ATGGAGCTCATCAAGATTATGCTGATCATAGCAACAACCATGGCTTTGTCCATCACTCTCATGACCGTGAACAGGGTCGGTCGTGAAGAAGAAGAGAAACCCTCTTTCGTGTCGGAAAAGCACCCCCTCTACGACGAGAAAAATCTGCTTCTGCCTTCGAAAAGGACAGCTACACGAACTCGACATGCTGCAACAACAAGTGCATGGACTTGTCCGAGGACAAGCACAACTGCGGCGCCTGCAAGAAGAAATGCAAGTACACTGAGACGTGCTGCAGAGGAGGCTGCGTTGACACGAATTTCGATAAGAGGCACTGCGGAGGCTGCAACCAGCGGTGCCAAAAAGGCCAGTATTGTGTGTACGGCCTGTGTAACTATGCCTGATTCATCTCATAAGGGCCGTCCGCTAACGGATTGTGAGTACTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Stig1

Protein Analysis

143

Amino Acids

15.45

Weight (kDa)

9.59

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50720 AT4G26880 AT5G55110
fragaria_vesca FvH4_3g23190
malus_domestica MD03G1197000.v1.1 MD11G1213000.v1.1
prunus_persica Prupe.4G218300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0040831
rosa_laevigata RLG00000034006
rosa_multiflora Rmu_sc0001579.1_g000006
rosa_roxburghii Rroxscaffold_1G00039870
rosa_rugosa Rorug05G0188000
rosa_samantha Rh5BG278600 Rh5CG310600 Rh5DG286500
rosa_wichuraiana Rw5G025710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 235, 335
AciI CCGC 4 cut(s) 233, 317, 333, 404
AcsI RAATTY 1 cut(s) 297
AcyI GRCGYC 1 cut(s) 236
AfaI GTAC 3 cut(s) 259, 361, 422
AfiI CCNNNNNNNGG 1 cut(s) 72
AjiI CACGTC 1 cut(s) 269
AluBI AGCT 2 cut(s) 7, 170
AluI AGCT 2 cut(s) 7, 170
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 259
AoxI GGCC 3 cut(s) 345, 363, 397
ApeKI GCWGC 4 cut(s) 188, 272, 284, 323
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AspLEI GCGC 1 cut(s) 238
AspS9I GGNCC 1 cut(s) 397
AsuII TTCGAA 1 cut(s) 158
BanI GGYRCC 2 cut(s) 235, 335
BanII GRGCYC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 4 cut(s) 175, 259, 271, 310
BccI CCATC 1 cut(s) 59
BceAI ACGGC 2 cut(s) 378, 384
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 259
BfmI CTRYAG 1 cut(s) 273
BfoI RGCGCY 1 cut(s) 239
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 5 cut(s) 189, 234, 273, 285, 324
BlsI GCNGC 5 cut(s) 190, 235, 274, 286, 325
BmcAI AGTACT 1 cut(s) 422
BmgBI CACGTC 1 cut(s) 269
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 2 cut(s) 237, 337
BplI GAGNNNNNCTC 2 cut(s) 86, 118
Bpu14I TTCGAA 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 39, 215
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 348
BseDI CCNNGG 2 cut(s) 39, 215
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 348
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 4 cut(s) 175, 259, 271, 310
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 347, 365, 399
BshNI GGYRCC 2 cut(s) 235, 335
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 259
BsmBI CGTCTC 1 cut(s) 259
BsnI GGCC 3 cut(s) 347, 365, 399
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 9
Bsp143I GATC 1 cut(s) 24
Bsp19I CCATGG 1 cut(s) 39
BspACI CCGC 4 cut(s) 233, 317, 333, 404
BspANI GGCC 3 cut(s) 347, 365, 399
BspCNI CTCAG 1 cut(s) 255
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 2 cut(s) 237, 337
BspMAI CTGCAG 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 158
BspT107I GGYRCC 2 cut(s) 235, 335
BsrI ACTGG 1 cut(s) 348
BssECI CCNNGG 2 cut(s) 39, 215
BssMI GATC 1 cut(s) 24
BssNI GRCGYC 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 236
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 263
BstDSI CCRYGG 1 cut(s) 39
BstH2I RGCGCY 1 cut(s) 239
BstHHI GCGC 1 cut(s) 238
BstKTI GATC 1 cut(s) 27
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 1 cut(s) 24
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 4 cut(s) 239, 281, 323, 344
BstNSI RCATGY 1 cut(s) 188
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 4 cut(s) 175, 259, 271, 310
BsuRI GGCC 3 cut(s) 347, 365, 399
BtgI CCRYGG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 269
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 260, 312
Cac8I GCNNGC 1 cut(s) 240
CciI TCATGA 1 cut(s) 60
CfoI GCGC 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 397
Csp6I GTAC 3 cut(s) 258, 360, 421
CviAII CATG 4 cut(s) 40, 61, 185, 204
CviJI RGCY 8 cut(s) 7, 44, 170, 284, 323, 347, 365, 399
CviKI_1 RGCY 8 cut(s) 7, 44, 170, 284, 323, 347, 365, 399
CviQI GTAC 3 cut(s) 258, 360, 421
DdeI CTNAG 1 cut(s) 263
DinI GGCGCC 1 cut(s) 237
DpnI GATC 1 cut(s) 26
DpnII GATC 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 9
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 9
EgeI GGCGCC 1 cut(s) 237
EheI GGCGCC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 39
Esp3I CGTCTC 1 cut(s) 259
FaeI CATG 4 cut(s) 43, 64, 188, 207
FaiI YATR 8 cut(s) 20, 29, 41, 62, 186, 205, 377, 393
FatI CATG 4 cut(s) 39, 60, 184, 203
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 5 cut(s) 189, 234, 273, 285, 324
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 5 cut(s) 189, 234, 273, 285, 324
GlaI GCGC 1 cut(s) 237
GluI GCNGC 5 cut(s) 189, 234, 273, 285, 324
HaeII RGCGCY 1 cut(s) 239
HaeIII GGCC 3 cut(s) 347, 365, 399
HhaI GCGC 1 cut(s) 238
Hin1I GRCGYC 1 cut(s) 236
Hin1II CATG 4 cut(s) 43, 64, 188, 207
Hin6I GCGC 1 cut(s) 236
HinP1I GCGC 1 cut(s) 236
HincII GTYRAC 1 cut(s) 291
HindII GTYRAC 1 cut(s) 291
HinfI GANTC 1 cut(s) 383
Hpy166II GTNNAC 4 cut(s) 70, 260, 291, 360
Hpy188I TCNGA 2 cut(s) 115, 216
Hpy188III TCNNGA 4 cut(s) 13, 61, 83, 426
Hpy8I GTNNAC 4 cut(s) 70, 260, 291, 360
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 165
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 1 cut(s) 268
HpyCH4V TGCA 6 cut(s) 191, 203, 242, 254, 275, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 281, 323, 344
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 1 cut(s) 268
Hsp92I GRCGYC 1 cut(s) 236
Hsp92II CATG 4 cut(s) 43, 64, 188, 207
HspAI GCGC 1 cut(s) 236
KasI GGCGCC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 6 cut(s) 58, 252, 343, 361, 379, 393
Lsp1109I GCAGC 4 cut(s) 175, 259, 271, 310
MaeII ACGT 1 cut(s) 268
MaeIII GTNAC 1 cut(s) 370
MalI GATC 1 cut(s) 26
MboI GATC 1 cut(s) 24
MboII GAAGA 4 cut(s) 98, 101, 104, 258
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 1 cut(s) 297
Mly113I GGCGCC 1 cut(s) 236
MmeI TCCRAC 1 cut(s) 93
MnlI CCTC 7 cut(s) 112, 137, 210, 271, 274, 302, 313
MslI CAYNNNNRTG 2 cut(s) 59, 65
MspA1I CMGCKG 1 cut(s) 333
MwoI GCNNNNNNNGC 4 cut(s) 239, 281, 323, 344
NarI GGCGCC 1 cut(s) 236
NcoI CCATGG 1 cut(s) 39
NdeII GATC 1 cut(s) 24
NlaIII CATG 4 cut(s) 43, 64, 188, 207
NlaIV GGNNCC 2 cut(s) 237, 337
NspI RCATGY 1 cut(s) 188
NspV TTCGAA 1 cut(s) 158
PagI TCATGA 1 cut(s) 60
PfeI GAWTC 1 cut(s) 383
PkrI GCNGC 5 cut(s) 190, 235, 274, 286, 325
PluTI GGCGCC 1 cut(s) 239
Psp124BI GAGCTC 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 237, 337
PspPI GGNCC 1 cut(s) 397
PstI CTGCAG 1 cut(s) 277
RsaI GTAC 3 cut(s) 259, 361, 422
RsaNI GTAC 3 cut(s) 258, 360, 421
RseI CAYNNNNRTG 2 cut(s) 59, 65
SacI GAGCTC 1 cut(s) 9
SatI GCNGC 5 cut(s) 189, 234, 273, 285, 324
Sau3AI GATC 1 cut(s) 24
Sau96I GGNCC 1 cut(s) 397
ScaI AGTACT 1 cut(s) 422
SduI GDGCHC 1 cut(s) 9
SetI ASST 3 cut(s) 9, 172, 271
SfcI CTRYAG 1 cut(s) 273
SfoI GGCGCC 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 158
SmiMI CAYNNNNRTG 2 cut(s) 59, 65
Sse9I AATT 1 cut(s) 297
SsiI CCGC 4 cut(s) 233, 317, 333, 404
SspDI GGCGCC 1 cut(s) 235
SstI GAGCTC 1 cut(s) 9
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 1 cut(s) 271
TaqI TCGA 3 cut(s) 158, 181, 302
TaqII GACCGA 1 cut(s) 68
TasI AATT 1 cut(s) 297
TatI WGTACW 2 cut(s) 257, 420
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 1 cut(s) 383
TscAI CASTG 2 cut(s) 267, 319
TseI GCWGC 4 cut(s) 188, 272, 284, 323
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 2 cut(s) 267, 319
XapI RAATTY 1 cut(s) 297
XceI RCATGY 1 cut(s) 188
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.