AT5G55940

emb2731 emb2731 (embryo defective 2731)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015248
Physical Location & Seq
Reverse (-)
22655998 .. 22658084
2087 bp
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UTR
Exon/CDS
Intron
AT5G55940.1

Sequence Viewer

Length: 627 bp
ATGGGAATGGGAAGTAACGGAGAATTGAAGTATGAGATCTCCCAGAACGCGTACATCAAACTCGTTCTTCACTCGCTGCGACACAAGACGGCGGCGGTCAACGGAGTCCTCGTCGGTAGAATCAGCCCTAAGGACGACGGAGTTGTAGAGATCTCAGATTCTGTGCCGCTCTTTCACTCTAACCTCGCTCTCCTTCCTCCTCTCGAGATCTCCCTCATCATGATAGAGGAGCATTATGTGGCTCAAGGTTTAAGTATTGTTGGATACTTTCATGCAAACGAGAGGTTTGATGACGTTGAGCTCTGTGGTGTGGCTAAAAACATTGGTGATCACATTTCTCGCTATTTCCCTCAGGCACCAATTCTCTTGTTGAACAACAAAAAGCTTGAAGCCTTATCAAAGGGTAAAGAGCGAAGCCCTGTGATGCAGCTCTGTGTGAAGGATGCTTCTAAGAACTGGAGAGTAGTTGGAGCAGATGGAGGAAGCAAGCTACTCTTAAAAGAGCCATCGGCCAATGTAGTTTTGTCAGATTACATTTCATCTGAGAAATGGAAGGACGTCACAGATGTTGATGATCATCTTGATGATGTAACAAAGGACTGGCTAAACCCTGGACTTTTCAACTGA

Protein Analysis

208

Amino Acids

22.98

Weight (kDa)

5.62

Isoelectric Point (pI)

36.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0172 PF03665 10 - 205 1.1e-57 Uncharacterised protein family (UPF0172)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 561
AccB1I GGYRCC 1 cut(s) 355
AccBSI CCGCTC 1 cut(s) 169
AccII CGCG 1 cut(s) 50
AciI CCGC 3 cut(s) 92, 95, 167
AcoI YGGCCR 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 1 cut(s) 53
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 4 cut(s) 28, 373, 389, 622
AjnI CCWGG 1 cut(s) 610
AluBI AGCT 4 cut(s) 301, 385, 430, 490
AluI AGCT 4 cut(s) 301, 385, 430, 490
Alw21I GWGCWC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 161
Ama87I CYCGRG 1 cut(s) 203
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 2 cut(s) 76, 427
AsuHPI GGTGA 1 cut(s) 338
AvaI CYCGRG 1 cut(s) 203
AxyI CCTNAGG 2 cut(s) 129, 351
BanI GGYRCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 63, 439
BccI CCATC 2 cut(s) 470, 514
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 612
BciVI GTATCC 1 cut(s) 257
BclI TGATCA 2 cut(s) 328, 574
BfuI GTATCC 1 cut(s) 257
BglII AGATCT 3 cut(s) 36, 150, 207
BisI GCNGC 4 cut(s) 77, 93, 167, 428
BlsI GCNGC 4 cut(s) 78, 94, 168, 429
Bme1390I CCNGG 1 cut(s) 612
BmeT110I CYCGRG 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 612
BmsI GCATC 2 cut(s) 414, 433
BpmI CTGGAG 1 cut(s) 478
BpuEI CTTGAG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 610
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bse1I ACTGG 2 cut(s) 461, 605
Bse21I CCTNAGG 2 cut(s) 129, 351
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 1 cut(s) 610
BseGI GGATG 1 cut(s) 448
BseJI GATNNNNATC 1 cut(s) 576
BseMII CTCAG 3 cut(s) 168, 365, 534
BseNI ACTGG 2 cut(s) 461, 605
BseRI GAGGAG 2 cut(s) 189, 242
BseXI GCAGC 2 cut(s) 63, 439
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 1 cut(s) 512
BshNI GGYRCC 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 303
BsiHKCI CYCGRG 1 cut(s) 203
BsnI GGCC 1 cut(s) 512
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 5 cut(s) 36, 150, 207, 328, 574
BspACI CCGC 3 cut(s) 92, 95, 167
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 3 cut(s) 167, 364, 535
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 1 cut(s) 357
BspT107I GGYRCC 1 cut(s) 355
BsrBI CCGCTC 1 cut(s) 169
BsrI ACTGG 2 cut(s) 461, 605
BssECI CCNNGG 1 cut(s) 610
BssMI GATC 5 cut(s) 36, 150, 207, 328, 574
BssNI GRCGYC 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 612
BstACI GRCGYC 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 488
BstDEI CTNAG 5 cut(s) 129, 154, 351, 450, 543
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 5 cut(s) 39, 153, 210, 331, 577
BstMBI GATC 5 cut(s) 36, 150, 207, 328, 574
BstNI CCWGG 1 cut(s) 612
BstSCI CCNGG 1 cut(s) 610
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 63, 439
BstX2I RGATCY 3 cut(s) 36, 150, 207
BstYI RGATCY 3 cut(s) 36, 150, 207
Bsu36I CCTNAGG 2 cut(s) 129, 351
BsuI GTATCC 1 cut(s) 257
BsuRI GGCC 1 cut(s) 512
BtsCI GGATG 1 cut(s) 448
Cac8I GCNNGC 1 cut(s) 488
CaiI CAGNNNCTG 1 cut(s) 161
CciI TCATGA 1 cut(s) 219
Csp6I GTAC 1 cut(s) 52
CviAII CATG 2 cut(s) 220, 272
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 5 cut(s) 129, 154, 351, 450, 543
DpnI GATC 5 cut(s) 38, 152, 209, 330, 576
DpnII GATC 5 cut(s) 36, 150, 207, 328, 574
EaeI YGGCCR 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 301
Eco24I GRGCYC 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 301
Eco81I CCTNAGG 2 cut(s) 129, 351
Eco88I CYCGRG 1 cut(s) 203
EcoICRI GAGCTC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 303
FaeI CATG 2 cut(s) 223, 275
FaiI YATR 4 cut(s) 33, 221, 237, 273
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FatI CATG 2 cut(s) 219, 271
FbaI TGATCA 2 cut(s) 328, 574
Fnu4HI GCNGC 4 cut(s) 77, 93, 167, 428
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 4 cut(s) 77, 93, 167, 428
GluI GCNGC 4 cut(s) 77, 93, 167, 428
GsuI CTGGAG 1 cut(s) 478
HaeIII GGCC 1 cut(s) 512
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 2 cut(s) 223, 275
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 105, 120, 158
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 157, 529, 544
Hpy188III TCNNGA 4 cut(s) 203, 205, 220, 581
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 116, 140
HpyAV CCTTC 3 cut(s) 203, 433, 547
HpyCH4IV ACGT 2 cut(s) 294, 558
HpyCH4V TGCA 2 cut(s) 275, 427
HpyF3I CTNAG 5 cut(s) 129, 154, 351, 450, 543
HpySE526I ACGT 2 cut(s) 294, 558
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 2 cut(s) 223, 275
Ksp22I TGATCA 2 cut(s) 328, 574
Kzo9I GATC 5 cut(s) 36, 150, 207, 328, 574
LmnI GCTCC 2 cut(s) 229, 470
LpnPI CCDG 6 cut(s) 56, 338, 432, 442, 586, 597
Lsp1109I GCAGC 2 cut(s) 63, 439
LweI GCATC 2 cut(s) 414, 433
MaeII ACGT 2 cut(s) 294, 558
MaeIII GTNAC 3 cut(s) 14, 559, 589
MalI GATC 5 cut(s) 38, 152, 209, 330, 576
MbiI CCGCTC 1 cut(s) 169
MboI GATC 5 cut(s) 36, 150, 207, 328, 574
MboII GAAGA 1 cut(s) 59
MflI RGATCY 3 cut(s) 36, 150, 207
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 2 cut(s) 23, 360
MluI ACGCGT 1 cut(s) 48
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 2 cut(s) 241, 448
MnlI CCTC 9 cut(s) 119, 194, 207, 210, 220, 224, 276, 360, 473
MseI TTAA 2 cut(s) 251, 497
MslI CAYNNNNRTG 1 cut(s) 582
MspR9I CCNGG 1 cut(s) 612
MvaI CCWGG 1 cut(s) 612
MvnI CGCG 1 cut(s) 50
NdeII GATC 5 cut(s) 36, 150, 207, 328, 574
NlaIII CATG 2 cut(s) 223, 275
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 559
PaeR7I CTCGAG 1 cut(s) 203
PagI TCATGA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 2 cut(s) 120, 158
PkrI GCNGC 4 cut(s) 78, 94, 168, 429
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 1 cut(s) 610
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 1 cut(s) 357
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 3 cut(s) 36, 150, 207
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 582
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 2 cut(s) 251, 497
SatI GCNGC 4 cut(s) 77, 93, 167, 428
Sau3AI GATC 5 cut(s) 36, 150, 207, 328, 574
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 1 cut(s) 612
SduI GDGCHC 1 cut(s) 303
SetI ASST 9 cut(s) 186, 250, 287, 297, 303, 387, 432, 492, 561
SfaNI GCATC 2 cut(s) 414, 433
Sfr274I CTCGAG 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 2 cut(s) 203, 243
SmoI CTYRAG 2 cut(s) 203, 243
Sse9I AATT 2 cut(s) 23, 360
SsiI CCGC 3 cut(s) 92, 95, 167
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 1 cut(s) 610
TaiI ACGT 2 cut(s) 297, 561
TaqI TCGA 1 cut(s) 204
TasI AATT 2 cut(s) 23, 360
TauI GCSGC 2 cut(s) 95, 169
TfiI GAWTC 2 cut(s) 120, 158
Tru1I TTAA 2 cut(s) 251, 497
Tru9I TTAA 2 cut(s) 251, 497
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 2 cut(s) 76, 427
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 2 cut(s) 260, 528
TspGWI ACGGA 3 cut(s) 33, 117, 153
XhoI CTCGAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.