RchiOBHm_Chr5g0076271

ER membrane protein complex subunit 8 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015248
Physical Location & Seq
Forward (+)
81954174 .. 81958316
4143 bp
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UTR
Exon/CDS
Intron
PRQ35095

Sequence Viewer

Length: 609 bp
ATGGGCGGCGAGTTGCGCTACGAGATCGCGCAGAACGCGTACATAAAGCTTGTGCTACACGCGCTCAAGCACAAGACCTCCGCCGTCAACGGCATCCTCGTCGGCCGCGTCTCCCCCAAAAACGACGTCGTCGAGATCACCGACTCCGTCCCTCTATTCCACTCCCAGCTTGGCCTCCTCCCCCAGCTCGAGATCTCCCTCATCCTCATTGAGGAGCATTTTGTTACTAAAGGACTGAGCATTGTGGGATATTTTCACGCAAACGAGAGGTTTGATGATCATGAGCTTGGAAGCCTCGCCAAGAATGTTGGTGATCACATTTATCGTTACCTTCCTCAAGCTGCAGTGCTCTTGTTAGATAACAAGAAGCTTGGAGCTTTGTCAAAGACCAAGGACCGGAGCCCCGTGGTCCAGCTTTACACAAAAGATGCATCGAGGATTTGGAAGCTAGTTGGATCAGATGGAAATCAGTTAACGATCAAGGAGCCCTCAGCAAATGTTGTTCTACTGGATCACATCTCAACTGAAAAATGGCAGGATGTTGTAGATTTTGATGATCACCTTGATGACATTAGCAAGGATTGGCTGAACCCAGAGCTCTTCAAGTAA

Protein Analysis

202

Amino Acids

22.7

Weight (kDa)

5.82

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0172 PF03665 7 - 200 2.8e-60 Uncharacterised protein family (UPF0172)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccII CGCG 4 cut(s) 29, 38, 62, 108
AciI CCGC 3 cut(s) 6, 81, 106
AclWI GGATC 2 cut(s) 463, 519
AcoI YGGCCR 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 2 cut(s) 211, 396
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 1 cut(s) 604
Alw21I GWGCWC 2 cut(s) 351, 600
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 2 cut(s) 463, 519
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 103, 172
ApeKI GCWGC 1 cut(s) 341
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
AspLEI GCGC 3 cut(s) 18, 31, 64
AspS9I GGNCC 2 cut(s) 394, 409
AsuHPI GGTGA 3 cut(s) 130, 323, 551
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 394, 409
BanII GRGCYC 3 cut(s) 404, 489, 600
Bbv12I GWGCWC 2 cut(s) 351, 600
BbvCI CCTCAGC 1 cut(s) 490
BbvI GCAGC 1 cut(s) 328
BccI CCATC 1 cut(s) 455
BceAI ACGGC 2 cut(s) 68, 106
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BclI TGATCA 3 cut(s) 277, 313, 556
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 342
BglII AGATCT 1 cut(s) 192
BisI GCNGC 3 cut(s) 7, 106, 342
BlsI GCNGC 3 cut(s) 8, 107, 343
Bme18I GGWCC 2 cut(s) 394, 409
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 394, 409
BmiI GGNNCC 2 cut(s) 401, 486
BmsI GCATC 3 cut(s) 102, 418, 440
Bpu10I CCTNAGC 1 cut(s) 490
BpuEI CTTGAG 2 cut(s) 50, 321
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 390, 405
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 396
Bse1I ACTGG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 390, 405
BseGI GGATG 3 cut(s) 93, 201, 544
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 2 cut(s) 211, 396
BseMII CTCAG 2 cut(s) 227, 504
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 2 cut(s) 167, 227
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 328
BseYI CCCAGC 2 cut(s) 165, 183
Bsh1236I CGCG 4 cut(s) 29, 38, 62, 108
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 105, 174
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 351, 600
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 2 cut(s) 211, 396
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 2 cut(s) 105, 174
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 4 cut(s) 351, 404, 489, 600
Bsp143I GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
BspACI CCGC 3 cut(s) 6, 81, 106
BspANI GGCC 2 cut(s) 105, 174
BspCNI CTCAG 2 cut(s) 228, 503
BspFNI CGCG 4 cut(s) 29, 38, 62, 108
BspHI TCATGA 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 401, 486
BspMAI CTGCAG 1 cut(s) 346
BspPI GGATC 2 cut(s) 463, 519
BspQI GCTCTTC 1 cut(s) 605
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 2 cut(s) 390, 405
BssMI GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 605
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 236, 490
BstDSI CCRYGG 1 cut(s) 405
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 93, 201, 544
BstFNI CGCG 4 cut(s) 29, 38, 62, 108
BstHHI GCGC 3 cut(s) 18, 31, 64
BstKTI GATC 9 cut(s) 27, 138, 195, 280, 316, 458, 480, 514, 559
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 15, 35, 61
BstSFI CTRYAG 1 cut(s) 342
BstUI CGCG 4 cut(s) 29, 38, 62, 108
BstV1I GCAGC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 105, 174
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 3 cut(s) 93, 201, 544
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 1 cut(s) 351
CciI TCATGA 1 cut(s) 280
CfoI GCGC 3 cut(s) 18, 31, 64
Cfr13I GGNCC 2 cut(s) 394, 409
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 281
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 236, 490
DpnI GATC 9 cut(s) 26, 137, 194, 279, 315, 457, 479, 513, 558
DpnII GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 598
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 3 cut(s) 404, 489, 600
Eco47I GGWCC 2 cut(s) 394, 409
Eco52I CGGCCG 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 3 cut(s) 404, 489, 600
ErhI CCWWGG 1 cut(s) 390
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 1 cut(s) 284
FaiI YATR 2 cut(s) 44, 282
FaqI GGGAC 1 cut(s) 134
FatI CATG 1 cut(s) 280
FbaI TGATCA 3 cut(s) 277, 313, 556
Fnu4HI GCNGC 3 cut(s) 7, 106, 342
FokI GGATG 3 cut(s) 80, 188, 551
FriOI GRGCYC 3 cut(s) 404, 489, 600
Fsp4HI GCNGC 3 cut(s) 7, 106, 342
FspBI CTAG 1 cut(s) 449
GlaI GCGC 3 cut(s) 17, 30, 63
GluI GCNGC 3 cut(s) 7, 106, 342
GsaI CCCAGC 2 cut(s) 169, 187
HaeIII GGCC 2 cut(s) 105, 174
HapII CCGG 1 cut(s) 397
HgaI GACGC 1 cut(s) 97
HhaI GCGC 3 cut(s) 18, 31, 64
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 284
Hin6I GCGC 3 cut(s) 16, 29, 62
HinP1I GCGC 3 cut(s) 16, 29, 62
HincII GTYRAC 2 cut(s) 88, 474
HindII GTYRAC 2 cut(s) 88, 474
HindIII AAGCTT 2 cut(s) 47, 368
HinfI GANTC 1 cut(s) 143
HpaI GTTAAC 1 cut(s) 474
HpaII CCGG 1 cut(s) 397
HphI GGTGA 3 cut(s) 130, 323, 551
Hpy166II GTNNAC 2 cut(s) 88, 474
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 133, 190, 281
Hpy8I GTNNAC 2 cut(s) 88, 474
Hpy99I CGWCG 4 cut(s) 104, 128, 131, 134
HpyAV CCTTC 1 cut(s) 341
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 2 cut(s) 344, 431
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 35, 61
HpyF3I CTNAG 2 cut(s) 236, 490
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 284
HspAI GCGC 3 cut(s) 16, 29, 62
Ksp22I TGATCA 3 cut(s) 277, 313, 556
KspAI GTTAAC 1 cut(s) 474
Kzo9I GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 4 cut(s) 214, 374, 399, 484
LpnPI CCDG 6 cut(s) 179, 197, 410, 425, 494, 521
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 3 cut(s) 102, 418, 440
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 2 cut(s) 223, 326
MalI GATC 9 cut(s) 26, 137, 194, 279, 315, 457, 479, 513, 558
MboI GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
MboII GAAGA 1 cut(s) 592
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 4 cut(s) 351, 404, 489, 600
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 433
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 1 cut(s) 473
MslI CAYNNNNRTG 1 cut(s) 564
MspI CCGG 1 cut(s) 397
MvnI CGCG 4 cut(s) 29, 38, 62, 108
MwoI GCNNNNNNNGC 3 cut(s) 15, 35, 61
NdeII GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
NlaIII CATG 1 cut(s) 284
NlaIV GGNNCC 2 cut(s) 401, 486
NsiI ATGCAT 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 188
PagI TCATGA 1 cut(s) 280
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 105, 129, 138
PflFI GACNNNGTC 2 cut(s) 128, 146
PkrI GCNGC 3 cut(s) 8, 107, 343
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 600
PspFI CCCAGC 2 cut(s) 165, 183
PspN4I GGNNCC 2 cut(s) 401, 486
PspPI GGNCC 2 cut(s) 394, 409
PstI CTGCAG 1 cut(s) 346
PsuI RGATCY 1 cut(s) 192
PsyI GACNNNGTC 2 cut(s) 128, 146
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 1 cut(s) 473
SatI GCNGC 3 cut(s) 7, 106, 342
Sau3AI GATC 9 cut(s) 24, 135, 192, 277, 313, 455, 477, 511, 556
Sau96I GGNCC 2 cut(s) 394, 409
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 4 cut(s) 351, 404, 489, 600
SfaNI GCATC 3 cut(s) 102, 418, 440
SfcI CTRYAG 1 cut(s) 342
Sfr274I CTCGAG 1 cut(s) 188
SinI GGWCC 2 cut(s) 394, 409
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 3 cut(s) 65, 188, 336
SmoI CTYRAG 3 cut(s) 65, 188, 336
SsiI CCGC 3 cut(s) 6, 81, 106
SspMI CTAG 1 cut(s) 449
SstI GAGCTC 1 cut(s) 600
StyI CCWWGG 1 cut(s) 390
TaiI ACGT 1 cut(s) 129
TaqI TCGA 3 cut(s) 132, 189, 434
TauI GCSGC 2 cut(s) 9, 108
Tru1I TTAA 1 cut(s) 473
Tru9I TTAA 1 cut(s) 473
TscAI CASTG 1 cut(s) 351
TseI GCWGC 1 cut(s) 341
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 1 cut(s) 351
Tth111I GACNNNGTC 2 cut(s) 128, 146
VpaK11BI GGWCC 2 cut(s) 394, 409
XagI CCTNNNNNAGG 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XhoI CTCGAG 1 cut(s) 188
XspI CTAG 1 cut(s) 449
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.