FvH4_3g42640

ER membrane protein complex subunit 8 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015248
Physical Location & Seq
Forward (+)
35622399 .. 35625565
3167 bp
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UTR
Exon/CDS
Intron
FvH4_3g42640.t1

Sequence Viewer

Length: 609 bp
ATGGGCGGCGAGTTACGCTACGAGATCGCACAGAACGCGTACATAAAGCTCGTACTCCACGCCCTCAAGCACAAGACCTCCGCCGTCAACGGCATCCTACTCGGCCGCGTTTCCCCTCAAAACGACGTCGTCGAGATCACCGACTCCGTCCCTCTCTTCCACTCCCAGCTCGGCCTCCTCCCCCAGCTCGAGATCTCCCTCATCCTCATTGAGGAGCATTTCGCTGCGAAAGGAGTGAGCATTGTCGGGTACTTCCACGCGAACGAGAGGTTTGATGATCAGGAGCTTGGGAGCCTGGCCAAGAATGTTGGCGATCACATTTATCGCTACCTTCCTCAAGCTGCGGTGCTCTTGTTAGATAACAAGAAGCTTGCAGCTTTGTCAAAAACTAAGGACCGGAGCCCCGTGGTTCAGCTTTACACGAAAGATGCTTCTAGGATTTGGAAATTAGTTGGATCAGATGGAAATCAGTTGACAATGAAGGAGCCCTCAGCCAATGTTGTTCTATTGGATCACATTTCAACTGAAAAATGGCAGGATGTTGTAGATTTTGATGATCACCTTGATGACATTAGCAAGGATTGGCTGAACCCAGAGCTCTTCAAGTAA

Protein Analysis

203

Amino Acids

22.67

Weight (kDa)

5.57

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0172 PF03665 7 - 200 4.5e-61 Uncharacterised protein family (UPF0172)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccII CGCG 3 cut(s) 38, 108, 260
AciI CCGC 4 cut(s) 6, 81, 106, 344
AclWI GGATC 2 cut(s) 463, 519
AcoI YGGCCR 2 cut(s) 103, 297
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 3 cut(s) 41, 54, 251
AfiI CCNNNNNNNGG 1 cut(s) 211
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 2 cut(s) 522, 604
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 9 cut(s) 49, 169, 187, 286, 341, 370, 377, 415, 598
AluI AGCT 9 cut(s) 49, 169, 187, 286, 341, 370, 377, 415, 598
Alw21I GWGCWC 2 cut(s) 351, 600
AlwI GGATC 2 cut(s) 463, 519
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 103, 172, 297
ApeKI GCWGC 3 cut(s) 224, 341, 374
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 130, 551
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 394
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 3 cut(s) 404, 489, 600
Bbv12I GWGCWC 2 cut(s) 351, 600
BbvCI CCTCAGC 1 cut(s) 490
BbvI GCAGC 3 cut(s) 211, 328, 386
BccI CCATC 1 cut(s) 455
BceAI ACGGC 2 cut(s) 68, 106
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 1 cut(s) 296
BclI TGATCA 2 cut(s) 277, 556
BfaI CTAG 1 cut(s) 435
BglII AGATCT 1 cut(s) 192
BisI GCNGC 5 cut(s) 7, 106, 225, 342, 375
BlsI GCNGC 5 cut(s) 8, 107, 226, 343, 376
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 3 cut(s) 293, 401, 486
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 2 cut(s) 102, 418
Bpu10I CCTNAGC 1 cut(s) 490
BpuEI CTTGAG 2 cut(s) 50, 321
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 405
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 1 cut(s) 405
BseGI GGATG 3 cut(s) 93, 201, 544
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 504
BseRI GAGGAG 2 cut(s) 167, 227
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 3 cut(s) 211, 328, 386
BseYI CCCAGC 2 cut(s) 165, 183
Bsh1236I CGCG 3 cut(s) 38, 108, 260
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 3 cut(s) 105, 174, 299
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 351, 600
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 211
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 3 cut(s) 105, 174, 299
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 4 cut(s) 351, 404, 489, 600
Bsp143I GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
BspACI CCGC 4 cut(s) 6, 81, 106, 344
BspANI GGCC 3 cut(s) 105, 174, 299
BspCNI CTCAG 1 cut(s) 503
BspFNI CGCG 3 cut(s) 38, 108, 260
BspLI GGNNCC 3 cut(s) 293, 401, 486
BspPI GGATC 2 cut(s) 463, 519
BspQI GCTCTTC 1 cut(s) 605
BssECI CCNNGG 1 cut(s) 405
BssMI GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
BssNI GRCGYC 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 296
Bst6I CTCTTC 2 cut(s) 161, 605
BstACI GRCGYC 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 372
BstDEI CTNAG 2 cut(s) 390, 490
BstDSI CCRYGG 1 cut(s) 405
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 3 cut(s) 93, 201, 544
BstFNI CGCG 3 cut(s) 38, 108, 260
BstKTI GATC 8 cut(s) 27, 138, 195, 280, 316, 458, 514, 559
BstMBI GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 15, 35
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 1 cut(s) 294
BstUI CGCG 3 cut(s) 38, 108, 260
BstV1I GCAGC 3 cut(s) 211, 328, 386
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 3 cut(s) 105, 174, 299
BtgI CCRYGG 1 cut(s) 405
BtsCI GGATG 3 cut(s) 93, 201, 544
Cac8I GCNNGC 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 394
Csp6I GTAC 3 cut(s) 40, 53, 250
CviQI GTAC 3 cut(s) 40, 53, 250
DdeI CTNAG 2 cut(s) 390, 490
DpnI GATC 8 cut(s) 26, 137, 194, 279, 315, 457, 513, 558
DpnII GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
EaeI YGGCCR 2 cut(s) 103, 297
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 161, 605
EarI CTCTTC 2 cut(s) 161, 605
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 598
EclXI CGGCCG 1 cut(s) 103
Eco24I GRGCYC 3 cut(s) 404, 489, 600
Eco47I GGWCC 1 cut(s) 394
Eco52I CGGCCG 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 598
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 598
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 294
EcoT38I GRGCYC 3 cut(s) 404, 489, 600
FaiI YATR 1 cut(s) 44
FaqI GGGAC 1 cut(s) 134
FbaI TGATCA 2 cut(s) 277, 556
Fnu4HI GCNGC 5 cut(s) 7, 106, 225, 342, 375
FokI GGATG 3 cut(s) 80, 188, 551
FriOI GRGCYC 3 cut(s) 404, 489, 600
Fsp4HI GCNGC 5 cut(s) 7, 106, 225, 342, 375
FspBI CTAG 1 cut(s) 435
GluI GCNGC 5 cut(s) 7, 106, 225, 342, 375
GsaI CCCAGC 2 cut(s) 169, 187
HaeIII GGCC 3 cut(s) 105, 174, 299
HapII CCGG 1 cut(s) 397
Hin1I GRCGYC 1 cut(s) 126
HincII GTYRAC 2 cut(s) 88, 474
HindII GTYRAC 2 cut(s) 88, 474
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 397
HphI GGTGA 2 cut(s) 130, 551
Hpy166II GTNNAC 2 cut(s) 88, 474
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 133, 190, 281
Hpy8I GTNNAC 2 cut(s) 88, 474
Hpy99I CGWCG 3 cut(s) 128, 131, 134
HpyAV CCTTC 2 cut(s) 341, 475
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 35
HpyF3I CTNAG 2 cut(s) 390, 490
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Ksp22I TGATCA 2 cut(s) 277, 556
Kzo9I GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 5 cut(s) 214, 283, 291, 399, 484
LpnPI CCDG 7 cut(s) 179, 197, 266, 281, 308, 410, 521
Lsp1109I GCAGC 3 cut(s) 211, 328, 386
LweI GCATC 2 cut(s) 102, 418
MaeI CTAG 1 cut(s) 435
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 12
MalI GATC 8 cut(s) 26, 137, 194, 279, 315, 457, 513, 558
MboI GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
MboII GAAGA 2 cut(s) 148, 592
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 4 cut(s) 351, 404, 489, 600
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 446
MluI ACGCGT 1 cut(s) 36
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 433
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MslI CAYNNNNRTG 1 cut(s) 564
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 3 cut(s) 38, 108, 260
MwoI GCNNNNNNNGC 2 cut(s) 15, 35
NdeII GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
NlaIV GGNNCC 3 cut(s) 293, 401, 486
NmeAIII GCCGAG 2 cut(s) 81, 150
PaeR7I CTCGAG 1 cut(s) 188
PciSI GCTCTTC 1 cut(s) 605
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 138
PflFI GACNNNGTC 2 cut(s) 128, 146
PkrI GCNGC 5 cut(s) 8, 107, 226, 343, 376
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 2 cut(s) 165, 183
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 293, 401, 486
PspPI GGNCC 1 cut(s) 394
PsuI RGATCY 1 cut(s) 192
PsyI GACNNNGTC 2 cut(s) 128, 146
RsaI GTAC 3 cut(s) 41, 54, 251
RsaNI GTAC 3 cut(s) 40, 53, 250
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 605
SatI GCNGC 5 cut(s) 7, 106, 225, 342, 375
Sau3AI GATC 8 cut(s) 24, 135, 192, 277, 313, 455, 511, 556
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 4 cut(s) 351, 404, 489, 600
SfaNI GCATC 2 cut(s) 102, 418
Sfr274I CTCGAG 1 cut(s) 188
SinI GGWCC 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 3 cut(s) 65, 188, 336
SmoI CTYRAG 3 cut(s) 65, 188, 336
Sse9I AATT 1 cut(s) 446
SsiI CCGC 4 cut(s) 6, 81, 106, 344
SspMI CTAG 1 cut(s) 435
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 1 cut(s) 294
TaiI ACGT 1 cut(s) 129
TaqI TCGA 2 cut(s) 132, 189
TasI AATT 1 cut(s) 446
TauI GCSGC 2 cut(s) 9, 108
TseI GCWGC 3 cut(s) 224, 341, 374
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 136
Tth111I GACNNNGTC 2 cut(s) 128, 146
VpaK11BI GGWCC 1 cut(s) 394
XagI CCTNNNNNAGG 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 188
XspI CTAG 1 cut(s) 435
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.