Rorug05G0446500

ER membrane protein complex subunit 8 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015248
Physical Location & Seq
Reverse (-)
61802371 .. 61803774
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0446500.1

Sequence Viewer

Length: 1404 bp
ATGTTCCATTCAAGAGCCAAAGCTTTAAATCAAGCACAATCAAACCTTCTCCTCCATTGGAGCAATCGCACAAAACTCCAACCATACCACACTCTCCGTCGACCCAACAAACCGAAGACCAAGCCTCGCCGCGAACGGCCTCACAGAGACGCCGCAAAGCCTCGCAAGCCCATACCTTTCGTCTCCGATGTCAAAGAAGTTCAGGGCCCAGAAGATGCCTTGTCTCTCTTCCGAGAGTACCACGAAATGGGCTTCAAACACGACTACCCCTCTTACTCTGCTCTCATCTATAAGCTGGCTCGTAATCGAAACTTCGAAGCCGTTGAGACCATTCTTGGCCATGTACGAGACAGAAACATTCACTGCAAAGATACCCTTTTCATTGCTCTAATTCAACATTATGGGAAGGCCAAGTTGGTAGAGAAAGCCATTGAGCTTTTCCACCAAATCAATTCTTTTAATTGCGTCCGTACATTGCAGTCTTTCAATTCACTGCTTAATGTGCTTGTTGATAGTGACAGGTTTTCCGATGCCGATGAGATCTTCGGTCGGTGTAATCAAATGGGTTTTCGGCCAAATTCGATTTCGTATAACATAATCATAAAGGGGTGGCTCAAGAAAGGTGAATTGGATGAAGCACGCAAGGTGTTCGATGAAATGCTTGAGAGAAAAGTGCAGCCTAGTGTGGTGACGTATAACAGTCTGATAGGGTTCTTCGGTAGAAAGGGTGAGTTGGAGGAAGCTACTGGTTTGCTCGAGGATATGAGACAGAAAGGGAAGTACCCGAATGCTGTAACATATGCATTATTGATGGAAGGTTTGTGCATGGTAGGAAAGTATGAGGAAGCAAAGAAGATGATGTTTGATATGGAGTATCGGGGTTGTAAACCACGGCTTGTGAATTATGGTGTTTTGATAAGTGATCTTGGAAGAAGAGGGAAGATTGAGGCGGCAAAATCTTTACTGCAGGAAATGAAGAAAAGGCGGTTTAAGCCGGATGTTGTGATTTATAACATATTGATTAATTTTCTGTGCAAGGAAGGTAGGGCTATAGAGGCTTATAAAGTCTTGATTGAAATGCAAGTTGGAGGTTTCGAGCCTAATGCGGCCACATACAGGATGATGGTTGATGGGTTTTGTCAGATTGAAGATTTTGAGGGAGGGCTGAAGGTTTTGATTGCCATGTTGAAAAGTAAACACTGTCCTCGGTTGAAAACATTTGAGTGCTTGGTTACAGGACTATTGAAGTGCGGGAATGTTGATGGTGCTTGTTTTGTCTTGGAGGAGATGGAAAACAGAAATATGCAATTATGTTGCGAAGCTTGGGAAGCACCAGTTGTTGATGCTTGTGGTAAGGACATTGTTGCAGGTGAGAAAATGACTGAAGTCATATCTGCCCACTAA

Protein Analysis

467

Amino Acids

53.62

Weight (kDa)

8.99

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 135 - 272 6.6e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 161 - 203 2.8e-06 PPR repeat family
PPR_3 PF13812 181 - 241 6.7e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 188 - 220 2.6e-09 PPR repeat
PPR_2 PF13041 192 - 236 6.6e-16 PPR repeat family
PPR PF01535 195 - 224 4e-07 PPR repeat
PPR_1 PF12854 225 - 256 3.4e-08 PPR repeat
PPR_2 PF13041 227 - 275 1.9e-14 PPR repeat family
PPR PF01535 230 - 259 1e-06 PPR repeat
PPR_3 PF13812 251 - 310 2.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 262 - 306 5.3e-07 PPR repeat family
PPR_2 PF13041 299 - 335 2.4e-06 PPR repeat family
PPR_long PF17177 309 - 437 1.9e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 330 - 361 3e-10 PPR repeat
PPR_2 PF13041 332 - 381 2.3e-16 PPR repeat family
PPR PF01535 335 - 364 7.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1011, 1062
AarI CACCTGC 1 cut(s) 1358
Acc36I ACCTGC 1 cut(s) 1358
AccB7I CCANNNNNTGG 1 cut(s) 247
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 132
AciI CCGC 6 cut(s) 130, 153, 950, 985, 1106, 1251
AcoI YGGCCR 3 cut(s) 337, 572, 1107
AcsI RAATTY 1 cut(s) 577
AcuI CTGAAG 2 cut(s) 1187, 1404
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 4 cut(s) 239, 345, 472, 782
AfiI CCNNNNNNNGG 2 cut(s) 247, 1116
AgsI TTSAA 9 cut(s) 12, 256, 395, 487, 1076, 1148, 1189, 1213, 1246
AjuI GAANNNNNNNTTGG 8 cut(s) 398, 430, 568, 600, 611, 643, 1068, 1100
AluBI AGCT 5 cut(s) 23, 295, 436, 743, 1322
AluI AGCT 5 cut(s) 23, 295, 436, 743, 1322
Alw26I GTCTC 6 cut(s) 141, 187, 228, 320, 342, 760
Ama87I CYCGRG 1 cut(s) 755
AoxI GGCC 6 cut(s) 137, 205, 337, 408, 572, 1107
ApaI GGGCCC 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 676
ApoI RAATTY 1 cut(s) 577
AseI ATTAAT 1 cut(s) 1023
AspS9I GGNCC 2 cut(s) 205, 206
AsuHPI GGTGA 4 cut(s) 635, 700, 740, 1382
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 339
BanII GRGCYC 1 cut(s) 209
BbsI GAAGAC 1 cut(s) 122
BbvI GCAGC 1 cut(s) 688
BccI CCATC 5 cut(s) 805, 1117, 1124, 1256, 1282
BceAI ACGGC 3 cut(s) 152, 305, 908
BcgI CGANNNNNNTGC 4 cut(s) 631, 665, 1085, 1119
BcoDI GTCTC 6 cut(s) 141, 187, 228, 320, 342, 760
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 2 cut(s) 965, 1050
BfuAI ACCTGC 1 cut(s) 1358
BglII AGATCT 1 cut(s) 540
BisI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
BlsI GCNGC 5 cut(s) 131, 154, 678, 952, 1108
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 2 cut(s) 205, 206
BmiI GGNNCC 1 cut(s) 207
BmsI GCATC 3 cut(s) 205, 520, 1333
BoxI GACNNNNGTC 1 cut(s) 1385
BpiI GAAGAC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 2 cut(s) 599, 683
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 2 cut(s) 890, 1205
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 1116
Bse1I ACTGG 2 cut(s) 751, 1334
Bse3DI GCAATG 2 cut(s) 381, 473
BseDI CCNNGG 2 cut(s) 890, 1205
BseGI GGATG 3 cut(s) 637, 1003, 1125
BseLI CCNNNNNNNGG 2 cut(s) 247, 1116
BseMI GCAATG 2 cut(s) 381, 473
BseNI ACTGG 2 cut(s) 751, 1334
BseRI GAGGAG 2 cut(s) 41, 1298
BseSI GKGCMC 1 cut(s) 209
BseXI GCAGC 1 cut(s) 688
BsgI GTGCAG 1 cut(s) 695
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BsiEI CGRYCG 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 1 cut(s) 995
BslI CCNNNNNNNGG 2 cut(s) 247, 1116
BsmAI GTCTC 6 cut(s) 141, 187, 228, 320, 342, 760
BsmBI CGTCTC 2 cut(s) 141, 187
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 755
Bsp119I TTCGAA 1 cut(s) 315
Bsp120I GGGCCC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 209
Bsp143I GATC 2 cut(s) 540, 922
BspACI CCGC 6 cut(s) 130, 153, 950, 985, 1106, 1251
BspANI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 969
BspMI ACCTGC 1 cut(s) 1358
BspT104I TTCGAA 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 320
BsrDI GCAATG 2 cut(s) 381, 473
BsrI ACTGG 2 cut(s) 751, 1334
BssECI CCNNGG 2 cut(s) 890, 1205
BssMI GATC 2 cut(s) 540, 922
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 2 cut(s) 701, 1202
Bst6I CTCTTC 2 cut(s) 233, 928
BstACI GRCGYC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 3 cut(s) 167, 297, 640
BstDSI CCRYGG 1 cut(s) 890
BstF5I GGATG 3 cut(s) 637, 1003, 1125
BstFNI CGCG 1 cut(s) 132
BstKTI GATC 2 cut(s) 543, 925
BstMAI GTCTC 6 cut(s) 141, 187, 228, 320, 342, 760
BstMBI GATC 2 cut(s) 540, 922
BstMCI CGRYCG 1 cut(s) 550
BstMWI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
BstPAI GACNNNNGTC 1 cut(s) 1385
BstSFI CTRYAG 2 cut(s) 965, 1050
BstSLI GKGCMC 1 cut(s) 209
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 122
BstX2I RGATCY 1 cut(s) 540
BstYI RGATCY 1 cut(s) 540
BsuRI GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
BtgI CCRYGG 1 cut(s) 890
BtsCI GGATG 3 cut(s) 637, 1003, 1125
BtsI GCAGTG 2 cut(s) 361, 491
BtsIMutI CAGTG 3 cut(s) 361, 491, 1198
BveI ACCTGC 1 cut(s) 1358
Cac8I GCNNGC 3 cut(s) 167, 297, 640
Cfr13I GGNCC 2 cut(s) 205, 206
CseI GACGC 2 cut(s) 158, 454
Csp6I GTAC 4 cut(s) 238, 344, 471, 781
CviAII CATG 3 cut(s) 341, 826, 1183
CviQI GTAC 4 cut(s) 238, 344, 471, 781
DpnI GATC 2 cut(s) 542, 924
DpnII GATC 2 cut(s) 540, 922
DraI TTTAAA 1 cut(s) 27
EaeI YGGCCR 3 cut(s) 337, 572, 1107
Eam1104I CTCTTC 2 cut(s) 233, 928
EarI CTCTTC 2 cut(s) 233, 928
Eco24I GRGCYC 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 320
Eco57I CTGAAG 2 cut(s) 1187, 1404
Eco88I CYCGRG 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 805
EcoT38I GRGCYC 1 cut(s) 209
Esp3I CGTCTC 2 cut(s) 141, 187
FaeI CATG 3 cut(s) 344, 829, 1186
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FatI CATG 3 cut(s) 340, 825, 1182
FauI CCCGC 1 cut(s) 1244
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
FokI GGATG 3 cut(s) 644, 1010, 1132
FriOI GRGCYC 1 cut(s) 209
Fsp4HI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
FspBI CTAG 1 cut(s) 681
GluI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
HaeIII GGCC 6 cut(s) 139, 207, 339, 410, 574, 1109
HapII CCGG 1 cut(s) 995
HgaI GACGC 2 cut(s) 158, 454
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 3 cut(s) 344, 829, 1186
HincII GTYRAC 1 cut(s) 101
HindII GTYRAC 1 cut(s) 101
HindIII AAGCTT 2 cut(s) 21, 1320
HpaII CCGG 1 cut(s) 995
HphI GGTGA 4 cut(s) 635, 700, 740, 1382
Hpy166II GTNNAC 3 cut(s) 101, 887, 1196
Hpy188I TCNGA 5 cut(s) 187, 233, 529, 705, 1143
Hpy188III TCNNGA 3 cut(s) 12, 616, 1069
Hpy8I GTNNAC 3 cut(s) 101, 887, 1196
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 5 cut(s) 56, 400, 809, 1034, 1162
HpyCH4III ACNGT 2 cut(s) 701, 1202
HpyCH4IV ACGT 1 cut(s) 692
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
HpySE526I ACGT 1 cut(s) 692
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 3 cut(s) 344, 829, 1186
Kzo9I GATC 2 cut(s) 540, 922
LmnI GCTCC 1 cut(s) 60
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 3 cut(s) 205, 520, 1333
MaeI CTAG 1 cut(s) 681
MaeII ACGT 1 cut(s) 692
MaeIII GTNAC 4 cut(s) 515, 688, 793, 1231
MalI GATC 2 cut(s) 542, 924
MboI GATC 2 cut(s) 540, 922
MflI RGATCY 1 cut(s) 540
MhlI GDGCHC 1 cut(s) 209
MlsI TGGCCA 1 cut(s) 339
MluCI AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
MluNI TGGCCA 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 103, 714, 1066
Mox20I TGGCCA 1 cut(s) 339
Mph1103I ATGCAT 1 cut(s) 805
MscI TGGCCA 1 cut(s) 339
MseI TTAA 5 cut(s) 26, 459, 498, 990, 1023
MslI CAYNNNNRTG 1 cut(s) 1222
Msp20I TGGCCA 1 cut(s) 339
MspI CCGG 1 cut(s) 995
Mva1269I GAATGC 1 cut(s) 793
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 5 cut(s) 166, 502, 991, 1055, 1328
NdeI CATATG 1 cut(s) 799
NdeII GATC 2 cut(s) 540, 922
NlaIII CATG 3 cut(s) 344, 829, 1186
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 2 cut(s) 515, 688
NsiI ATGCAT 1 cut(s) 805
NspV TTCGAA 1 cut(s) 315
PaeR7I CTCGAG 1 cut(s) 755
PaqCI CACCTGC 1 cut(s) 1358
PctI GAATGC 1 cut(s) 793
PflMI CCANNNNNTGG 1 cut(s) 247
PkrI GCNGC 5 cut(s) 131, 154, 678, 952, 1108
PshAI GACNNNNGTC 1 cut(s) 1385
PshBI ATTAAT 1 cut(s) 1023
PsiI TTATAA 2 cut(s) 1011, 1062
PspN4I GGNNCC 1 cut(s) 207
PspOMI GGGCCC 1 cut(s) 205
PspPI GGNCC 2 cut(s) 205, 206
PspXI VCTCGAGB 1 cut(s) 755
PstI CTGCAG 1 cut(s) 969
PsuI RGATCY 1 cut(s) 540
RsaI GTAC 4 cut(s) 239, 345, 472, 782
RsaNI GTAC 4 cut(s) 238, 344, 471, 781
RseI CAYNNNNRTG 1 cut(s) 1222
SalI GTCGAC 1 cut(s) 99
SaqAI TTAA 5 cut(s) 26, 459, 498, 990, 1023
SatI GCNGC 5 cut(s) 130, 153, 677, 951, 1107
Sau3AI GATC 2 cut(s) 540, 922
Sau96I GGNCC 2 cut(s) 205, 206
SduI GDGCHC 1 cut(s) 209
SfaNI GCATC 3 cut(s) 205, 520, 1333
SfcI CTRYAG 2 cut(s) 965, 1050
Sfr274I CTCGAG 1 cut(s) 755
SfuI TTCGAA 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 755
SmiMI CAYNNNNRTG 1 cut(s) 1222
SmlI CTYRAG 3 cut(s) 614, 662, 755
SmoI CTYRAG 3 cut(s) 614, 662, 755
Sse9I AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
SsiI CCGC 6 cut(s) 130, 153, 950, 985, 1106, 1251
SspMI CTAG 1 cut(s) 681
TaaI ACNGT 2 cut(s) 701, 1202
TaiI ACGT 1 cut(s) 695
TaqI TCGA 7 cut(s) 100, 307, 315, 581, 651, 756, 1095
TaqII GACCGA 1 cut(s) 536
TasI AATT 9 cut(s) 390, 451, 460, 487, 577, 626, 901, 1024, 1307
TauI GCSGC 4 cut(s) 132, 155, 953, 1109
Tru1I TTAA 5 cut(s) 26, 459, 498, 990, 1023
Tru9I TTAA 5 cut(s) 26, 459, 498, 990, 1023
TscAI CASTG 3 cut(s) 368, 498, 1205
TseFI GTSAC 2 cut(s) 515, 688
TseI GCWGC 1 cut(s) 676
Tsp45I GTSAC 2 cut(s) 515, 688
TspDTI ATGAA 4 cut(s) 370, 648, 669, 989
TspGWI ACGGA 2 cut(s) 86, 458
TspRI CASTG 3 cut(s) 368, 498, 1205
Van91I CCANNNNNTGG 1 cut(s) 247
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 1 cut(s) 577
XhoI CTCGAG 1 cut(s) 755
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 1 cut(s) 681
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.