Rroxscaffold_1G00005590

ER membrane protein complex subunit 8 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015248
Physical Location & Seq
Reverse (-)
7538003 .. 7542133
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005590.1

Sequence Viewer

Length: 612 bp
ATGGGGGGCGAGTTGCGCTACGAGATCGCGCAGAACGCGTACATAAAGCTCGTGCTACACGCGCTCAAGCACAAGACCTCCGCCGTCAACGGCATCCTCGTCGGCCGCGTCTCCCCCAAAACGACGTCGTCGAGATCACCGACTCCGTCCCTCTCTTCCACTCCCAGCTCGGCCTCCTCCCCCAGCTCGAGATCTCCCTCATCCTCGTCAATTGAGGAGCATTTTGTTACTAAAGGACTGAGCATTGTGGGATATTTTCACGCAAACGAGAGGTTTGATGATCATGAGCTTGGAAGCCTCGCCAAGAATGTTGGTGATCACATTTATCGTTACCTTCCTCAAGCTGCAGTGCTCTTGTTGGATAACAAGAAGCTTGGAGCTTTGTCAAAGACCAAGGACCGGAGCCCCGTGGTCCAGCTTTACACAAAAGATGCATCGAGGATTTGGAAGTTAGTTGGATCAGATGGAAATCAGTTGACGATCAAGGAGCCCTCAGCAAATGTTGTTCTACTGGATCACATCTCAACTGAAAAATGGCAGGATGTTGTAGATTTTGATGATCACCTTGATGACATTAGCAAGGATTGGCTGAACCCAGAGCTCTTCAAGTAA

Protein Analysis

203

Amino Acids

22.38

Weight (kDa)

7.11

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0172 PF03665 7 - 201 3.5e-43 Uncharacterised protein family (UPF0172)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 128
AccII CGCG 4 cut(s) 29, 38, 62, 108
AciI CCGC 2 cut(s) 81, 106
AclWI GGATC 2 cut(s) 466, 522
AcoI YGGCCR 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 1 cut(s) 36
AgsI TTSAA 1 cut(s) 607
AluBI AGCT 9 cut(s) 49, 168, 186, 289, 344, 373, 380, 418, 601
AluI AGCT 9 cut(s) 49, 168, 186, 289, 344, 373, 380, 418, 601
Alw21I GWGCWC 2 cut(s) 354, 603
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 2 cut(s) 466, 522
Ama87I CYCGRG 1 cut(s) 187
AoxI GGCC 2 cut(s) 103, 171
ApeKI GCWGC 1 cut(s) 344
AspLEI GCGC 3 cut(s) 18, 31, 64
AspS9I GGNCC 2 cut(s) 397, 412
AsuHPI GGTGA 3 cut(s) 129, 326, 554
AvaI CYCGRG 1 cut(s) 187
AvaII GGWCC 2 cut(s) 397, 412
BanII GRGCYC 3 cut(s) 407, 492, 603
BauI CACGAG 1 cut(s) 50
Bbv12I GWGCWC 2 cut(s) 354, 603
BbvCI CCTCAGC 1 cut(s) 493
BbvI GCAGC 1 cut(s) 331
BccI CCATC 1 cut(s) 458
BceAI ACGGC 2 cut(s) 68, 106
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BclI TGATCA 3 cut(s) 280, 316, 559
BcoDI GTCTC 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 345
BglII AGATCT 1 cut(s) 191
BisI GCNGC 2 cut(s) 106, 345
BlsI GCNGC 2 cut(s) 107, 346
Bme18I GGWCC 2 cut(s) 397, 412
BmeT110I CYCGRG 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 397, 412
BmiI GGNNCC 2 cut(s) 404, 489
BmsI GCATC 3 cut(s) 102, 421, 443
Bpu10I CCTNAGC 1 cut(s) 493
BpuEI CTTGAG 2 cut(s) 50, 324
BsaBI GATNNNNATC 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 393, 408
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 393, 408
BseGI GGATG 3 cut(s) 93, 200, 547
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 1 cut(s) 399
BseMII CTCAG 2 cut(s) 230, 507
BseNI ACTGG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 166, 230
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 331
BseYI CCCAGC 2 cut(s) 164, 182
Bsh1236I CGCG 4 cut(s) 29, 38, 62, 108
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 2 cut(s) 105, 173
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 354, 603
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 1 cut(s) 400
BslFI GGGAC 1 cut(s) 133
BslI CCNNNNNNNGG 1 cut(s) 399
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 133
BsnI GGCC 2 cut(s) 105, 173
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 4 cut(s) 354, 407, 492, 603
Bsp143I GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
BspACI CCGC 2 cut(s) 81, 106
BspANI GGCC 2 cut(s) 105, 173
BspCNI CTCAG 2 cut(s) 231, 506
BspFNI CGCG 4 cut(s) 29, 38, 62, 108
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 2 cut(s) 404, 489
BspMAI CTGCAG 1 cut(s) 349
BspPI GGATC 2 cut(s) 466, 522
BspQI GCTCTTC 1 cut(s) 608
BsrI ACTGG 1 cut(s) 516
BssECI CCNNGG 2 cut(s) 393, 408
BssMI GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 50
Bst6I CTCTTC 2 cut(s) 160, 608
BstACI GRCGYC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 239, 493
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 3 cut(s) 93, 200, 547
BstFNI CGCG 4 cut(s) 29, 38, 62, 108
BstHHI GCGC 3 cut(s) 18, 31, 64
BstKTI GATC 9 cut(s) 27, 137, 194, 283, 319, 461, 483, 517, 562
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 3 cut(s) 15, 35, 61
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 4 cut(s) 29, 38, 62, 108
BstV1I GCAGC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 191
BstYI RGATCY 1 cut(s) 191
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 105, 173
BtgI CCRYGG 1 cut(s) 408
BtsCI GGATG 3 cut(s) 93, 200, 547
BtsI GCAGTG 1 cut(s) 354
BtsIMutI CAGTG 1 cut(s) 354
CciI TCATGA 1 cut(s) 283
CfoI GCGC 3 cut(s) 18, 31, 64
Cfr13I GGNCC 2 cut(s) 397, 412
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 284
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 239, 493
DpnI GATC 9 cut(s) 26, 136, 193, 282, 318, 460, 482, 516, 561
DpnII GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 160, 608
EarI CTCTTC 2 cut(s) 160, 608
EciI GGCGGA 1 cut(s) 70
Ecl136II GAGCTC 1 cut(s) 601
EclXI CGGCCG 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 3 cut(s) 407, 492, 603
Eco47I GGWCC 2 cut(s) 397, 412
Eco52I CGGCCG 1 cut(s) 103
Eco53kI GAGCTC 1 cut(s) 601
Eco88I CYCGRG 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 601
EcoT14I CCWWGG 1 cut(s) 393
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 3 cut(s) 407, 492, 603
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 1 cut(s) 287
FaiI YATR 2 cut(s) 44, 285
FaqI GGGAC 1 cut(s) 133
FatI CATG 1 cut(s) 283
FbaI TGATCA 3 cut(s) 280, 316, 559
Fnu4HI GCNGC 2 cut(s) 106, 345
FokI GGATG 3 cut(s) 80, 187, 554
FriOI GRGCYC 3 cut(s) 407, 492, 603
Fsp4HI GCNGC 2 cut(s) 106, 345
GlaI GCGC 3 cut(s) 17, 30, 63
GluI GCNGC 2 cut(s) 106, 345
GsaI CCCAGC 2 cut(s) 168, 186
HaeIII GGCC 2 cut(s) 105, 173
HapII CCGG 1 cut(s) 400
HgaI GACGC 1 cut(s) 97
HhaI GCGC 3 cut(s) 18, 31, 64
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 1 cut(s) 287
Hin6I GCGC 3 cut(s) 16, 29, 62
HinP1I GCGC 3 cut(s) 16, 29, 62
HincII GTYRAC 2 cut(s) 88, 477
HindII GTYRAC 2 cut(s) 88, 477
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 1 cut(s) 142
HpaII CCGG 1 cut(s) 400
HphI GGTGA 3 cut(s) 129, 326, 554
Hpy166II GTNNAC 2 cut(s) 88, 477
Hpy188I TCNGA 1 cut(s) 463
Hpy188III TCNNGA 3 cut(s) 132, 189, 284
Hpy8I GTNNAC 2 cut(s) 88, 477
Hpy99I CGWCG 4 cut(s) 104, 127, 130, 133
HpyAV CCTTC 1 cut(s) 344
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 2 cut(s) 347, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 35, 61
HpyF3I CTNAG 2 cut(s) 239, 493
HpySE526I ACGT 1 cut(s) 125
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 1 cut(s) 287
HspAI GCGC 3 cut(s) 16, 29, 62
Ksp22I TGATCA 3 cut(s) 280, 316, 559
Kzo9I GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
LguI GCTCTTC 1 cut(s) 608
LmnI GCTCC 4 cut(s) 217, 377, 402, 487
LpnPI CCDG 6 cut(s) 178, 196, 413, 428, 497, 524
Lsp1109I GCAGC 1 cut(s) 331
LweI GCATC 3 cut(s) 102, 421, 443
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 2 cut(s) 226, 329
MalI GATC 9 cut(s) 26, 136, 193, 282, 318, 460, 482, 516, 561
MboI GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
MboII GAAGA 2 cut(s) 147, 595
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 4 cut(s) 354, 407, 492, 603
MluCI AATT 1 cut(s) 210
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 1 cut(s) 136
MmeI TCCRAC 2 cut(s) 339, 436
Mph1103I ATGCAT 1 cut(s) 436
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 210
MvnI CGCG 4 cut(s) 29, 38, 62, 108
MwoI GCNNNNNNNGC 3 cut(s) 15, 35, 61
NdeII GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
NlaIII CATG 1 cut(s) 287
NlaIV GGNNCC 2 cut(s) 404, 489
NmeAIII GCCGAG 1 cut(s) 149
NsiI ATGCAT 1 cut(s) 436
PaeR7I CTCGAG 1 cut(s) 187
PagI TCATGA 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 608
PcsI WCGNNNNNNNCGW 4 cut(s) 96, 105, 128, 137
PflFI GACNNNGTC 2 cut(s) 127, 145
PkrI GCNGC 2 cut(s) 107, 346
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 603
PspFI CCCAGC 2 cut(s) 164, 182
PspN4I GGNNCC 2 cut(s) 404, 489
PspPI GGNCC 2 cut(s) 397, 412
PstI CTGCAG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 191
PsyI GACNNNGTC 2 cut(s) 127, 145
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 567
SacI GAGCTC 1 cut(s) 603
SapI GCTCTTC 1 cut(s) 608
SatI GCNGC 2 cut(s) 106, 345
Sau3AI GATC 9 cut(s) 24, 134, 191, 280, 316, 458, 480, 514, 559
Sau96I GGNCC 2 cut(s) 397, 412
SchI GAGTC 1 cut(s) 136
SduI GDGCHC 4 cut(s) 354, 407, 492, 603
SfaNI GCATC 3 cut(s) 102, 421, 443
SfcI CTRYAG 1 cut(s) 345
Sfr274I CTCGAG 1 cut(s) 187
SinI GGWCC 2 cut(s) 397, 412
SlaI CTCGAG 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 567
SmlI CTYRAG 3 cut(s) 65, 187, 339
SmoI CTYRAG 3 cut(s) 65, 187, 339
Sse9I AATT 1 cut(s) 210
SsiI CCGC 2 cut(s) 81, 106
SstI GAGCTC 1 cut(s) 603
StyI CCWWGG 1 cut(s) 393
TaiI ACGT 1 cut(s) 128
TaqI TCGA 3 cut(s) 131, 188, 437
TasI AATT 1 cut(s) 210
TauI GCSGC 1 cut(s) 108
TscAI CASTG 1 cut(s) 354
TseI GCWGC 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 354
Tth111I GACNNNGTC 2 cut(s) 127, 145
VpaK11BI GGWCC 2 cut(s) 397, 412
XhoI CTCGAG 1 cut(s) 187
ZraI GACGTC 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.