MD03G1030300.v1.1

ER membrane protein complex subunit 8 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015248
Physical Location & Seq
Reverse (-)
2451692 .. 2454764
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1030300.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGGTGGCGAGTTACGCTACGAGATCGCACAAAATGCCTACATAAAGCTAGTGCTACACGCTCTTAAGCACAAGACCTCCGCGGTCAACGGCATCCTCCTCGGTCGCGTCTCCCCCCACAACGACGTCGTGGAGATCACCGATTCCGTCCCTCTCTTCCACTCCCACATCGGCCTCCTCCCCCAGCTCGAGATCTCCCTCATCCTGATAGAGGAGCACTTTGCTGCTAAAGGAGTGAGCATTGTGGGGTATTTCCATGCTAACGAGAGGTTTGACGATCACGAGCTCGGCGGCATTGCCAAGAACGTCGGCGATCACATTTATCGCTACCTTCCTCAAGCCGCAATTCTTTTGCTTGATAACAGGAAGCTTGAAGCTTTGTCGAAGACTAAGGACCGGAGCCCCGTAGTGCAGCTCTACACGAAGGATGCATCCAGGACTTGGAAGTTAGTTGGATCGGATGGAAACCAGTTGACCATCAAAGAGCCATCAGCAAATGTTGTTCTATTGGATTACATCTCCACCGAAAAATGGCAGGATGTTGTAGATTTCGATGATCACCTCGATGACATTAGCAAGGACTGGCTGAACCCAGAACTATTCAAGTAA

Protein Analysis

203

Amino Acids

22.76

Weight (kDa)

5.64

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UPF0172 PF03665 7 - 200 7e-61 Uncharacterised protein family (UPF0172)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015248)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55940
fragaria_vesca FvH4_3g42640
malus_domestica MD03G1030300.v1.1 MD11G1035300.v1.1
prunus_persica Prupe.6G026500_v2.0.a1
pyrus_communis pycom11g02930
rosa_chinensis RchiOBHm_Chr5g0076271 RchiOBHm_Chr5g0076591
rosa_laevigata RLG00000036612
rosa_multiflora Rmu_sc0005961.1_g000004
rosa_roxburghii Rroxscaffold_1G00005590
rosa_rugosa Rorug05G0446500
rosa_samantha Rh5AG501700 Rh5BG523500 Rh5CG547200
rosa_wichuraiana Rw0G015030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 129
AccB7I CCANNNNNTGG 1 cut(s) 441
AccII CGCG 2 cut(s) 83, 108
AciI CCGC 4 cut(s) 81, 83, 291, 342
AclWI GGATC 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 126
AfiI CCNNNNNNNGG 3 cut(s) 211, 441, 531
AflII CTTAAG 1 cut(s) 65
AgsI TTSAA 2 cut(s) 374, 604
AjnI CCWGG 1 cut(s) 434
AluBI AGCT 6 cut(s) 49, 187, 286, 370, 377, 415
AluI AGCT 6 cut(s) 49, 187, 286, 370, 377, 415
Alw21I GWGCWC 2 cut(s) 219, 288
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 2 cut(s) 224, 412
AspS9I GGNCC 1 cut(s) 394
AsuHPI GGTGA 2 cut(s) 130, 551
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 288, 404
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 1 cut(s) 392
Bbv12I GWGCWC 2 cut(s) 219, 288
BbvI GCAGC 2 cut(s) 211, 424
BccI CCATC 3 cut(s) 455, 485, 496
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 82, 116
BciT130I CCWGG 1 cut(s) 436
BclI TGATCA 1 cut(s) 556
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 65
BglII AGATCT 1 cut(s) 192
BisI GCNGC 4 cut(s) 225, 292, 342, 413
BlsI GCNGC 4 cut(s) 226, 293, 343, 414
Bme1390I CCNGG 1 cut(s) 436
Bme18I GGWCC 1 cut(s) 394
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 401
BmrFI CCNGG 1 cut(s) 436
BmsI GCATC 3 cut(s) 102, 418, 440
BpiI GAAGAC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 81, 100
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 441, 531
Bse1I ACTGG 2 cut(s) 469, 587
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 436
BseDI CCNNGG 2 cut(s) 81, 100
BseGI GGATG 6 cut(s) 93, 201, 431, 433, 466, 544
BseLI CCNNNNNNNGG 3 cut(s) 211, 441, 531
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 2 cut(s) 469, 587
BseRI GAGGAG 3 cut(s) 89, 167, 227
BseXI GCAGC 2 cut(s) 211, 424
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 431
Bsh1236I CGCG 2 cut(s) 83, 108
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 219, 288
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 3 cut(s) 211, 441, 531
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 1 cut(s) 174
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 3 cut(s) 219, 288, 404
Bsp143I GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
BspACI CCGC 4 cut(s) 81, 83, 291, 342
BspANI GGCC 1 cut(s) 174
BspFNI CGCG 2 cut(s) 83, 108
BspLI GGNNCC 1 cut(s) 401
BspPI GGATC 1 cut(s) 463
BspTI CTTAAG 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 294
BsrI ACTGG 2 cut(s) 469, 587
BssECI CCNNGG 2 cut(s) 81, 100
BssMI GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 281
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 126
BstAFI CTTAAG 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 35
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 81
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 6 cut(s) 93, 201, 431, 433, 466, 544
BstFNI CGCG 2 cut(s) 83, 108
BstKTI GATC 7 cut(s) 27, 138, 195, 280, 316, 458, 559
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 2 cut(s) 15, 35
BstNI CCWGG 1 cut(s) 436
BstSCI CCNGG 1 cut(s) 434
BstUI CGCG 2 cut(s) 83, 108
BstV1I GCAGC 2 cut(s) 211, 424
BstV2I GAAGAC 1 cut(s) 392
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 1 cut(s) 174
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 6 cut(s) 93, 201, 431, 433, 466, 544
Cfr13I GGNCC 1 cut(s) 394
Cfr42I CCGCGG 1 cut(s) 84
CseI GACGC 1 cut(s) 97
CviAII CATG 1 cut(s) 257
DdeI CTNAG 1 cut(s) 390
DpnI GATC 7 cut(s) 26, 137, 194, 279, 315, 457, 558
DpnII GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 286
Eco24I GRGCYC 2 cut(s) 288, 404
Eco47I GGWCC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 434
EcoT22I ATGCAT 1 cut(s) 433
EcoT38I GRGCYC 2 cut(s) 288, 404
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 1 cut(s) 260
FaiI YATR 2 cut(s) 44, 258
FaqI GGGAC 1 cut(s) 134
FatI CATG 1 cut(s) 256
FbaI TGATCA 1 cut(s) 556
Fnu4HI GCNGC 4 cut(s) 225, 292, 342, 413
FokI GGATG 6 cut(s) 80, 188, 418, 440, 473, 551
FriOI GRGCYC 2 cut(s) 288, 404
Fsp4HI GCNGC 4 cut(s) 225, 292, 342, 413
FspBI CTAG 1 cut(s) 50
GluI GCNGC 4 cut(s) 225, 292, 342, 413
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 174
HapII CCGG 1 cut(s) 397
HgaI GACGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 260
HincII GTYRAC 2 cut(s) 88, 474
HindII GTYRAC 2 cut(s) 88, 474
HindIII AAGCTT 2 cut(s) 368, 375
HinfI GANTC 1 cut(s) 143
HpaII CCGG 1 cut(s) 397
HphI GGTGA 2 cut(s) 130, 551
Hpy166II GTNNAC 2 cut(s) 88, 474
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 3 cut(s) 190, 205, 281
Hpy8I GTNNAC 2 cut(s) 88, 474
Hpy99I CGWCG 3 cut(s) 128, 131, 311
HpyAV CCTTC 2 cut(s) 341, 418
HpyCH4IV ACGT 2 cut(s) 126, 306
HpyCH4V TGCA 2 cut(s) 412, 431
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 35
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 2 cut(s) 126, 306
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 260
Ksp22I TGATCA 1 cut(s) 556
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
LmnI GCTCC 2 cut(s) 214, 399
LpnPI CCDG 9 cut(s) 197, 218, 349, 410, 421, 448, 482, 521, 568
Lsp1109I GCAGC 2 cut(s) 211, 424
LweI GCATC 3 cut(s) 102, 418, 440
MaeI CTAG 1 cut(s) 50
MaeII ACGT 2 cut(s) 126, 306
MaeIII GTNAC 1 cut(s) 12
MalI GATC 7 cut(s) 26, 137, 194, 279, 315, 457, 558
MboI GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
MboII GAAGA 2 cut(s) 148, 397
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 3 cut(s) 219, 288, 404
MluCI AATT 1 cut(s) 345
MmeI TCCRAC 1 cut(s) 433
Mph1103I ATGCAT 1 cut(s) 433
MseI TTAA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 1 cut(s) 83
MspCI CTTAAG 1 cut(s) 65
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 1 cut(s) 436
MvaI CCWGG 1 cut(s) 436
MvnI CGCG 2 cut(s) 83, 108
MwoI GCNNNNNNNGC 2 cut(s) 15, 35
NdeII GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
NlaIII CATG 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 401
NmeAIII GCCGAG 1 cut(s) 267
NsiI ATGCAT 1 cut(s) 433
PaeR7I CTCGAG 1 cut(s) 188
PfeI GAWTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 441
PfoI TCCNGGA 1 cut(s) 434
PkrI GCNGC 4 cut(s) 226, 293, 343, 414
Psp124BI GAGCTC 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 1 cut(s) 434
PspN4I GGNNCC 1 cut(s) 401
PspPI GGNCC 1 cut(s) 394
PsuI RGATCY 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 288
SacII CCGCGG 1 cut(s) 84
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 4 cut(s) 225, 292, 342, 413
Sau3AI GATC 7 cut(s) 24, 135, 192, 277, 313, 455, 556
Sau96I GGNCC 1 cut(s) 394
ScrFI CCNGG 1 cut(s) 436
SduI GDGCHC 3 cut(s) 219, 288, 404
SfaNI GCATC 3 cut(s) 102, 418, 440
Sfr274I CTCGAG 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 84
SgrBI CCGCGG 1 cut(s) 84
SinI GGWCC 1 cut(s) 394
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 3 cut(s) 65, 188, 336
SmoI CTYRAG 3 cut(s) 65, 188, 336
Sse9I AATT 1 cut(s) 345
SsiI CCGC 4 cut(s) 81, 83, 291, 342
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 434
TaiI ACGT 2 cut(s) 129, 309
TaqI TCGA 4 cut(s) 189, 383, 552, 564
TaqII GACCGA 1 cut(s) 92
TasI AATT 1 cut(s) 345
TauI GCSGC 2 cut(s) 294, 344
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TseI GCWGC 2 cut(s) 224, 412
TspGWI ACGGA 1 cut(s) 136
Van91I CCANNNNNTGG 1 cut(s) 441
Vha464I CTTAAG 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 394
XagI CCTNNNNNAGG 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 188
XspI CTAG 1 cut(s) 50
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.