AT5G56150

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012716
Physical Location & Seq
Forward (+)
22729015 .. 22731315
2301 bp
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UTR
Exon/CDS
Intron
AT5G56150.2

Sequence Viewer

Length: 447 bp
ATGGCGTCGAAAAGAATTAACAAAGAGCTTAGGGACTTGCAAAGAGATCCTCCTGTGTCCTGCAGTGCAGGTCCTACGGGAGATGATATGTTCCAATGGCAAGCAACTATCATGGGTCCAGCAGATAGCCCGTTTGCCGGAGGTGTGTTTCTTGTTACCATTCACTTCCCACCAGATTACCCTTTCAAGCCACCAAAGGTTGCCTTCCGGACCAAGGTTTATCACCCAAACATCAACAGTAATGGAAGTATCTGTCTTGACATTCTGAAAGAGCAGTGGAGCCCTGCACTTACCGTATCCAAGGTTCTTCTGTCGATATGCTCATTGCTGACGGATCCAAACCCTGACGATCCTTTGGTTCCTGAAATAGCGCACATCTACAAGACAGACCGAGTCAAGTACGAGTCCACAGCTCAATCCTGGACTCAGAAGTATGCAATGGGATGA

Protein Analysis

148

Amino Acids

16.48

Weight (kDa)

6.81

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 104 2.2e-39 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56150
fragaria_vesca FvH4_3g41701
malus_domestica MD03G1041600.v1.1
prunus_persica Prupe.6G033700_v2.0.a1 Prupe.6G033700_v2.0.a1
pyrus_communis pycom03g03230 pycom11g03660
rosa_chinensis RchiOBHm_Chr5g0074801
rosa_multiflora Rmu_sc0000909.1_g000007 Rmu_sc0005365.1_g000005
rosa_roxburghii Rroxscaffold_1G00006770
rosa_rugosa Rorug05G0433800 Rorug05G0433900
rosa_samantha Rh5AG491800 Rh5BG513200 Rh5CG536900 Rh5DG525300
rosa_wichuraiana Rw5G045550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AccIII TCCGGA 1 cut(s) 207
AclWI GGATC 4 cut(s) 41, 329, 342, 344
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 2 cut(s) 137, 214
AgsI TTSAA 1 cut(s) 187
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 2 cut(s) 28, 413
AluI AGCT 2 cut(s) 28, 413
AlwI GGATC 4 cut(s) 41, 329, 342, 344
Aor13HI TCCGGA 1 cut(s) 207
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 3 cut(s) 71, 116, 210
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 3 cut(s) 71, 116, 210
BamHI GGATCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 284
BciT130I CCWGG 1 cut(s) 421
BciVI GTATCC 1 cut(s) 307
BfmI CTRYAG 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 59
BfuI GTATCC 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 3 cut(s) 71, 116, 210
BmgT120I GGNCC 3 cut(s) 71, 116, 210
BmiI GGNNCC 4 cut(s) 117, 281, 336, 360
BmrFI CCNGG 1 cut(s) 421
Bpu10I CCTNAGC 1 cut(s) 29
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 213, 300
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 214
Bse3DI GCAATG 2 cut(s) 323, 444
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 2 cut(s) 213, 300
BseLI CCNNNNNNNGG 2 cut(s) 137, 214
BseMI GCAATG 2 cut(s) 323, 444
BseMII CTCAG 1 cut(s) 440
BsgI GTGCAG 2 cut(s) 87, 270
BsiSI CCGG 2 cut(s) 138, 208
BslFI GGGAC 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 137, 214
BsmFI GGGAC 1 cut(s) 47
Bsp1286I GDGCHC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 207
Bsp143I GATC 3 cut(s) 46, 334, 349
BspCNI CTCAG 1 cut(s) 439
BspEI TCCGGA 1 cut(s) 207
BspLI GGNNCC 4 cut(s) 117, 281, 336, 360
BspMAI CTGCAG 1 cut(s) 65
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 4 cut(s) 41, 329, 342, 344
BsrDI GCAATG 2 cut(s) 323, 444
BssECI CCNNGG 2 cut(s) 213, 300
BssMI GATC 3 cut(s) 46, 334, 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 213, 300
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 2 cut(s) 239, 295
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 2 cut(s) 29, 426
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 3 cut(s) 49, 337, 352
BstMBI GATC 3 cut(s) 46, 334, 349
BstNI CCWGG 1 cut(s) 421
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 61
BstX2I RGATCY 2 cut(s) 46, 334
BstYI RGATCY 2 cut(s) 46, 334
BsuI GTATCC 1 cut(s) 307
BtsI GCAGTG 2 cut(s) 70, 281
BtsIMutI CAGTG 2 cut(s) 70, 281
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 71, 116, 210
Csp6I GTAC 1 cut(s) 400
CviAII CATG 1 cut(s) 112
CviJI RGCY 5 cut(s) 28, 129, 190, 282, 413
CviKI_1 RGCY 5 cut(s) 28, 129, 190, 282, 413
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 29, 426
DpnI GATC 3 cut(s) 48, 336, 351
DpnII GATC 3 cut(s) 46, 334, 349
Eco130I CCWWGG 2 cut(s) 213, 300
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 71, 116, 210
EcoO109I RGGNCCY 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 2 cut(s) 213, 300
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 213, 300
FaeI CATG 1 cut(s) 115
FaiI YATR 4 cut(s) 89, 113, 319, 435
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 1 cut(s) 47
FatI CATG 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 284
GlaI GCGC 1 cut(s) 372
HapII CCGG 2 cut(s) 138, 208
HhaI GCGC 1 cut(s) 373
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HinfI GANTC 3 cut(s) 393, 404, 424
HpaII CCGG 2 cut(s) 138, 208
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 408
Hpy188I TCNGA 2 cut(s) 267, 429
Hpy188III TCNNGA 3 cut(s) 208, 257, 362
Hpy8I GTNNAC 1 cut(s) 408
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 214
HpyCH4III ACNGT 2 cut(s) 239, 295
HpyCH4V TGCA 5 cut(s) 40, 63, 68, 287, 437
HpyF3I CTNAG 2 cut(s) 29, 426
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 1 cut(s) 371
Kpn2I TCCGGA 1 cut(s) 207
Kzo9I GATC 3 cut(s) 46, 334, 349
LmnI GCTCC 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 154
MalI GATC 3 cut(s) 48, 336, 351
MboI GATC 3 cut(s) 46, 334, 349
MboII GAAGA 1 cut(s) 299
MflI RGATCY 2 cut(s) 46, 334
MhlI GDGCHC 1 cut(s) 284
MluCI AATT 1 cut(s) 15
MlyI GAGTC 3 cut(s) 402, 413, 418
MnlI CCTC 2 cut(s) 60, 134
MroI TCCGGA 1 cut(s) 207
MseI TTAA 1 cut(s) 18
MspI CCGG 2 cut(s) 138, 208
MspR9I CCNGG 1 cut(s) 421
MvaI CCWGG 1 cut(s) 421
NdeII GATC 3 cut(s) 46, 334, 349
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 4 cut(s) 117, 281, 336, 360
PflFI GACNNNGTC 1 cut(s) 392
PfoI TCCNGGA 1 cut(s) 419
PleI GAGTC 3 cut(s) 401, 412, 418
PpsI GAGTC 3 cut(s) 401, 412, 418
PpuMI RGGWCCY 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 71
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PspN4I GGNNCC 4 cut(s) 117, 281, 336, 360
PspPI GGNCC 3 cut(s) 71, 116, 210
PspPPI RGGWCCY 1 cut(s) 71
PsrI GAACNNNNNNTAC 2 cut(s) 288, 320
PstI CTGCAG 1 cut(s) 65
PsuI RGATCY 2 cut(s) 46, 334
PsyI GACNNNGTC 1 cut(s) 392
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 3 cut(s) 46, 334, 349
Sau96I GGNCC 3 cut(s) 71, 116, 210
SchI GAGTC 3 cut(s) 402, 413, 418
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 284
SetI ASST 7 cut(s) 30, 73, 145, 201, 219, 306, 415
SfcI CTRYAG 1 cut(s) 61
SinI GGWCC 3 cut(s) 71, 116, 210
Sse9I AATT 1 cut(s) 15
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 2 cut(s) 213, 300
TaaI ACNGT 2 cut(s) 239, 295
TaqI TCGA 2 cut(s) 8, 314
TaqII GACCGA 1 cut(s) 405
TasI AATT 1 cut(s) 15
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 70, 281
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 2 cut(s) 70, 281
Tth111I GACNNNGTC 1 cut(s) 392
VpaK11BI GGWCC 3 cut(s) 71, 116, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.