Rroxscaffold_1G00006770

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012716
Physical Location & Seq
Forward (+)
8773581 .. 8775666
2086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006770.1

Sequence Viewer

Length: 447 bp
ATGGCTTCGAAGCGGATCAGCAAGGAGTTGAAGGACCTGCAGAAAGACCCGCCAGCTTCCTGCAGTGCTGGCCCTGTTGCTGAGGATATGTTCCATTGGCAAGCTACGATCATGGGTCCAGCTGACAGCCCATTTGCTGGAGGTGTTTTCCTTGTATCCATTCACTTCCCACCTGATTACCCTTTCAAGCCACCTAAGGTTGCTTTCCGGACAAAAGTGTACCATCCCAACATTAATAGCAATGGTAGTATCTGCCTTGACATCCTCAAAGAGCAGTGGAGCCCTGCCCTGACCATTTCCAAGGTCCTTCTCTCCATATGCTCACTGCTGACAGATCCAAACCCAGATGATCCTCTGGTGCCTGAGATTGCTCACATGTACAAGACTGATAGAGTCAAGTATGAGACTACTGCTCGATCCTGGACTCAAAAGTATGCTATGGGTTAG

Protein Analysis

148

Amino Acids

16.46

Weight (kDa)

7.72

Isoelectric Point (pI)

46.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 2.4e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56150
fragaria_vesca FvH4_3g41701
malus_domestica MD03G1041600.v1.1
prunus_persica Prupe.6G033700_v2.0.a1 Prupe.6G033700_v2.0.a1
pyrus_communis pycom03g03230 pycom11g03660
rosa_chinensis RchiOBHm_Chr5g0074801
rosa_multiflora Rmu_sc0000909.1_g000007 Rmu_sc0005365.1_g000005
rosa_roxburghii Rroxscaffold_1G00006770
rosa_rugosa Rorug05G0433800 Rorug05G0433900
rosa_samantha Rh5AG491800 Rh5BG513200 Rh5CG536900 Rh5DG525300
rosa_wichuraiana Rw5G045550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 137
AccIII TCCGGA 1 cut(s) 207
AciI CCGC 2 cut(s) 13, 50
AclWI GGATC 4 cut(s) 23, 329, 344, 411
AfaI GTAC 2 cut(s) 221, 380
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 2 cut(s) 31, 187
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 3 cut(s) 56, 104, 122
AluI AGCT 3 cut(s) 56, 104, 122
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 4 cut(s) 23, 329, 344, 411
Aor13HI TCCGGA 1 cut(s) 207
AoxI GGCC 1 cut(s) 70
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AseI ATTAAT 1 cut(s) 234
AspS9I GGNCC 4 cut(s) 34, 71, 116, 304
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 3 cut(s) 34, 116, 304
AxyI CCTNAGG 1 cut(s) 195
BanI GGYRCC 1 cut(s) 358
BanII GRGCYC 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 81
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 421
BciVI GTATCC 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 398
BfmI CTRYAG 2 cut(s) 38, 61
BfuAI ACCTGC 1 cut(s) 45
BfuI GTATCC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 421
Bme18I GGWCC 3 cut(s) 34, 116, 304
BmgT120I GGNCC 4 cut(s) 34, 71, 116, 304
BmiI GGNNCC 3 cut(s) 117, 281, 360
BmrFI CCNGG 1 cut(s) 421
BpmI CTGGAG 1 cut(s) 159
Bpu10I CCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse21I CCTNAGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 247
BseAI TCCGGA 1 cut(s) 207
BseBI CCWGG 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 2 cut(s) 223, 261
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 2 cut(s) 72, 354
BshFI GGCC 1 cut(s) 72
BshNI GGYRCC 1 cut(s) 358
BsiSI CCGG 1 cut(s) 208
BslI CCNNNNNNNGG 1 cut(s) 137
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 1 cut(s) 72
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 284
Bsp13I TCCGGA 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 5 cut(s) 15, 108, 334, 349, 416
BspACI CCGC 2 cut(s) 13, 50
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 2 cut(s) 73, 355
BspEI TCCGGA 1 cut(s) 207
BspLI GGNNCC 3 cut(s) 117, 281, 360
BspMAI CTGCAG 2 cut(s) 42, 65
BspMI ACCTGC 1 cut(s) 45
BspPI GGATC 4 cut(s) 23, 329, 344, 411
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 5 cut(s) 15, 108, 334, 349, 416
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 421
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 54, 70, 102
BstDEI CTNAG 3 cut(s) 81, 195, 363
BstF5I GGATG 2 cut(s) 223, 261
BstKTI GATC 5 cut(s) 18, 111, 337, 352, 419
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 5 cut(s) 15, 108, 334, 349, 416
BstMWI GCNNNNNNNGC 1 cut(s) 69
BstNI CCWGG 1 cut(s) 421
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 419
BstSFI CTRYAG 2 cut(s) 38, 61
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 195
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 2 cut(s) 223, 261
BtsI GCAGTG 3 cut(s) 70, 281, 323
BtsIMutI CAGTG 3 cut(s) 70, 281, 323
BveI ACCTGC 1 cut(s) 45
Cac8I GCNNGC 3 cut(s) 54, 70, 102
Cfr13I GGNCC 4 cut(s) 34, 71, 116, 304
Csp6I GTAC 2 cut(s) 220, 379
CviAII CATG 2 cut(s) 112, 376
CviJI RGCY 8 cut(s) 5, 56, 72, 104, 122, 129, 190, 282
CviKI_1 RGCY 8 cut(s) 5, 56, 72, 104, 122, 129, 190, 282
CviQI GTAC 2 cut(s) 220, 379
DdeI CTNAG 3 cut(s) 81, 195, 363
DpnI GATC 5 cut(s) 17, 110, 336, 351, 418
DpnII GATC 5 cut(s) 15, 108, 334, 349, 416
Eco130I CCWWGG 1 cut(s) 300
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 3 cut(s) 34, 116, 304
Eco81I CCTNAGG 1 cut(s) 195
EcoO109I RGGNCCY 2 cut(s) 34, 304
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 1 cut(s) 300
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 2 cut(s) 115, 379
FaiI YATR 8 cut(s) 89, 113, 317, 319, 377, 402, 435, 440
FatI CATG 2 cut(s) 111, 375
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 1 cut(s) 317
FokI GGATG 2 cut(s) 210, 248
FriOI GRGCYC 1 cut(s) 284
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 208
Hin1II CATG 2 cut(s) 115, 379
HinfI GANTC 2 cut(s) 393, 424
HpaII CCGG 1 cut(s) 208
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 208
Hpy8I GTNNAC 1 cut(s) 220
HpyAV CCTTC 2 cut(s) 25, 317
HpyCH4V TGCA 2 cut(s) 40, 63
HpyF10VI GCNNNNNNNGC 1 cut(s) 69
HpyF3I CTNAG 3 cut(s) 81, 195, 363
Hsp92II CATG 2 cut(s) 115, 379
Kpn2I TCCGGA 1 cut(s) 207
Kzo9I GATC 5 cut(s) 15, 108, 334, 349, 416
LmnI GCTCC 1 cut(s) 279
MalI GATC 5 cut(s) 17, 110, 336, 351, 418
MboI GATC 5 cut(s) 15, 108, 334, 349, 416
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 284
MlyI GAGTC 2 cut(s) 402, 418
MnlI CCTC 4 cut(s) 76, 134, 275, 363
MroI TCCGGA 1 cut(s) 207
MseI TTAA 1 cut(s) 234
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 208
MspR9I CCNGG 1 cut(s) 421
MvaI CCWGG 1 cut(s) 421
MwoI GCNNNNNNNGC 1 cut(s) 69
NdeI CATATG 1 cut(s) 317
NdeII GATC 5 cut(s) 15, 108, 334, 349, 416
NlaIII CATG 2 cut(s) 115, 379
NlaIV GGNNCC 3 cut(s) 117, 281, 360
NspI RCATGY 1 cut(s) 379
NspV TTCGAA 1 cut(s) 8
PciI ACATGT 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 137
PfoI TCCNGGA 1 cut(s) 419
PleI GAGTC 2 cut(s) 401, 418
PpsI GAGTC 2 cut(s) 401, 418
PpuMI RGGWCCY 2 cut(s) 34, 304
PscI ACATGT 1 cut(s) 375
PshBI ATTAAT 1 cut(s) 234
Psp5II RGGWCCY 2 cut(s) 34, 304
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PspN4I GGNNCC 3 cut(s) 117, 281, 360
PspPI GGNCC 4 cut(s) 34, 71, 116, 304
PspPPI RGGWCCY 2 cut(s) 34, 304
PstI CTGCAG 2 cut(s) 42, 65
PsuI RGATCY 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 2 cut(s) 221, 380
RsaNI GTAC 2 cut(s) 220, 379
SaqAI TTAA 1 cut(s) 234
Sau3AI GATC 5 cut(s) 15, 108, 334, 349, 416
Sau96I GGNCC 4 cut(s) 34, 71, 116, 304
SchI GAGTC 2 cut(s) 402, 418
ScrFI CCNGG 1 cut(s) 421
SduI GDGCHC 1 cut(s) 284
SetI ASST 9 cut(s) 39, 58, 106, 124, 145, 175, 196, 201, 306
SfcI CTRYAG 2 cut(s) 38, 61
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 3 cut(s) 34, 116, 304
SsiI CCGC 2 cut(s) 13, 50
StyD4I CCNGG 1 cut(s) 419
StyI CCWWGG 1 cut(s) 300
TaqI TCGA 2 cut(s) 8, 415
TatI WGTACW 1 cut(s) 378
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 3 cut(s) 70, 281, 330
TspRI CASTG 3 cut(s) 70, 281, 330
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 34, 116, 304
VspI ATTAAT 1 cut(s) 234
XceI RCATGY 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.