MD03G1041600.v1.1

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012716
Physical Location & Seq
Forward (+)
3284471 .. 3287214
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1041600.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCTTCGAAGCGGATCAACAAGGAGTTGAAGGACCTTCAGAAAGACCCGCCCGCTTCTTGCAGTGCAGGTCCCGTTGCTGATGATATGTTTCACTGGCAAGCAACTATTATGGGTCCAGGCGACAGCCCATTTTCTGGTGGTGTTTTTCTTGTATCCATTCATTTCCCGCCTGATTACCCTTTCAAGCCACCCAAGGTTGCCTTCCGAACAAAAGTTTACCATCCGAACATCAATAGCAATGGTAGTATCTGCCTTGACATCCTCAAGGAGCAGTGGAGTCCTGCCCTGACCATTTCCAAGGTCCTCCTCTCCATTTGCTCCCTTCTGACAGATCCAAACCCTGACGATCCTCTGGTGCCTGAGATTGCGCACATGTACAAGACTGATAGACAGAAATACGAGGCCACTGCTCGATCGTGGACCCAGAAGTATGCCATGGGCTAG

Protein Analysis

149

Amino Acids

16.48

Weight (kDa)

7.72

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 5 - 141 2e-54 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56150
fragaria_vesca FvH4_3g41701
malus_domestica MD03G1041600.v1.1
prunus_persica Prupe.6G033700_v2.0.a1 Prupe.6G033700_v2.0.a1
pyrus_communis pycom03g03230 pycom11g03660
rosa_chinensis RchiOBHm_Chr5g0074801
rosa_multiflora Rmu_sc0000909.1_g000007 Rmu_sc0005365.1_g000005
rosa_roxburghii Rroxscaffold_1G00006770
rosa_rugosa Rorug05G0433800 Rorug05G0433900
rosa_samantha Rh5AG491800 Rh5BG513200 Rh5CG536900 Rh5DG525300
rosa_wichuraiana Rw5G045550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 372
Acc36I ACCTGC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 137
AciI CCGC 4 cut(s) 13, 50, 54, 170
AclWI GGATC 3 cut(s) 23, 329, 344
AcuI CTGAAG 1 cut(s) 23
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 1 cut(s) 137
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 2 cut(s) 31, 187
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 188, 220
AlwI GGATC 3 cut(s) 23, 329, 344
AoxI GGCC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 373
AspS9I GGNCC 5 cut(s) 34, 71, 116, 304, 423
AsuII TTCGAA 1 cut(s) 8
AvaII GGWCC 5 cut(s) 34, 71, 116, 304, 423
BanI GGYRCC 1 cut(s) 358
BccI CCATC 1 cut(s) 231
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 1 cut(s) 120
BciVI GTATCC 1 cut(s) 166
BfaI CTAG 1 cut(s) 445
BfuAI ACCTGC 1 cut(s) 59
BfuI GTATCC 1 cut(s) 166
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 5 cut(s) 34, 71, 116, 304, 423
BmgT120I GGNCC 5 cut(s) 34, 71, 116, 304, 423
BmiI GGNNCC 4 cut(s) 73, 117, 360, 425
BmrFI CCNGG 1 cut(s) 120
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 195, 300, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 3 cut(s) 195, 300, 438
BseGI GGATG 2 cut(s) 223, 261
BseLI CCNNNNNNNGG 1 cut(s) 137
BseMI GCAATG 1 cut(s) 247
BseMII CTCAG 1 cut(s) 354
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 1 cut(s) 407
BshNI GGYRCC 1 cut(s) 358
BsiEI CGRYCG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 1 cut(s) 137
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 378
Bsp143I GATC 4 cut(s) 15, 334, 349, 416
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 4 cut(s) 13, 50, 54, 170
BspANI GGCC 1 cut(s) 407
BspCNI CTCAG 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 73, 117, 360, 425
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 3 cut(s) 23, 329, 344
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 378
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 3 cut(s) 195, 300, 438
BssMI GATC 4 cut(s) 15, 334, 349, 416
BssT1I CCWWGG 3 cut(s) 195, 300, 438
Bst2UI CCWGG 1 cut(s) 120
BstAUI TGTACA 1 cut(s) 378
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 2 cut(s) 54, 102
BstDEI CTNAG 1 cut(s) 363
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 2 cut(s) 223, 261
BstHHI GCGC 1 cut(s) 373
BstKTI GATC 4 cut(s) 18, 337, 352, 419
BstMBI GATC 4 cut(s) 15, 334, 349, 416
BstMCI CGRYCG 1 cut(s) 419
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 1 cut(s) 407
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 2 cut(s) 223, 261
BtsI GCAGTG 3 cut(s) 70, 281, 408
BtsIMutI CAGTG 4 cut(s) 70, 94, 281, 408
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 2 cut(s) 54, 102
CfoI GCGC 1 cut(s) 373
Cfr13I GGNCC 5 cut(s) 34, 71, 116, 304, 423
Csp6I GTAC 1 cut(s) 379
CviAII CATG 2 cut(s) 376, 439
CviJI RGCY 5 cut(s) 5, 129, 190, 407, 444
CviKI_1 RGCY 5 cut(s) 5, 129, 190, 407, 444
CviQI GTAC 1 cut(s) 379
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 17, 336, 351, 418
DpnII GATC 4 cut(s) 15, 334, 349, 416
Eco130I CCWWGG 3 cut(s) 195, 300, 438
Eco47I GGWCC 5 cut(s) 34, 71, 116, 304, 423
Eco57I CTGAAG 1 cut(s) 23
EcoO109I RGGNCCY 3 cut(s) 34, 71, 304
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 3 cut(s) 195, 300, 438
ErhI CCWWGG 3 cut(s) 195, 300, 438
FaeI CATG 2 cut(s) 379, 442
FaiI YATR 5 cut(s) 89, 113, 377, 435, 440
FalI AAGNNNNNCTT 2 cut(s) 188, 220
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 375, 438
FauI CCCGC 3 cut(s) 57, 61, 177
FokI GGATG 2 cut(s) 210, 248
FspBI CTAG 1 cut(s) 445
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 1 cut(s) 372
HaeIII GGCC 1 cut(s) 407
HhaI GCGC 1 cut(s) 373
Hin1II CATG 2 cut(s) 379, 442
Hin6I GCGC 1 cut(s) 371
HinP1I GCGC 1 cut(s) 371
HinfI GANTC 1 cut(s) 280
Hpy166II GTNNAC 2 cut(s) 220, 423
Hpy188I TCNGA 4 cut(s) 42, 209, 228, 330
Hpy8I GTNNAC 2 cut(s) 220, 423
HpyAV CCTTC 4 cut(s) 25, 47, 214, 335
HpyCH4V TGCA 2 cut(s) 63, 68
HpyF3I CTNAG 1 cut(s) 363
Hsp92II CATG 2 cut(s) 379, 442
HspAI GCGC 1 cut(s) 371
Kzo9I GATC 4 cut(s) 15, 334, 349, 416
LmnI GCTCC 2 cut(s) 271, 326
MaeI CTAG 1 cut(s) 445
MalI GATC 4 cut(s) 17, 336, 351, 418
MboI GATC 4 cut(s) 15, 334, 349, 416
MflI RGATCY 1 cut(s) 334
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 5 cut(s) 275, 317, 320, 363, 397
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
NcoI CCATGG 1 cut(s) 438
NdeII GATC 4 cut(s) 15, 334, 349, 416
NlaIII CATG 2 cut(s) 379, 442
NlaIV GGNNCC 4 cut(s) 73, 117, 360, 425
NsbI TGCGCA 1 cut(s) 372
NspI RCATGY 1 cut(s) 379
NspV TTCGAA 1 cut(s) 8
PciI ACATGT 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 137
Ple19I CGATCG 1 cut(s) 419
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
PpuMI RGGWCCY 3 cut(s) 34, 71, 304
PscI ACATGT 1 cut(s) 375
Psp5II RGGWCCY 3 cut(s) 34, 71, 304
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 4 cut(s) 73, 117, 360, 425
PspPI GGNCC 5 cut(s) 34, 71, 116, 304, 423
PspPPI RGGWCCY 3 cut(s) 34, 71, 304
PsuI RGATCY 1 cut(s) 334
PvuI CGATCG 1 cut(s) 419
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
Sau3AI GATC 4 cut(s) 15, 334, 349, 416
Sau96I GGNCC 5 cut(s) 34, 71, 116, 304, 423
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 1 cut(s) 120
SetI ASST 4 cut(s) 39, 73, 201, 306
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 5 cut(s) 34, 71, 116, 304, 423
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SsiI CCGC 4 cut(s) 13, 50, 54, 170
SspMI CTAG 1 cut(s) 445
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 3 cut(s) 195, 300, 438
TaqI TCGA 2 cut(s) 8, 415
TatI WGTACW 1 cut(s) 378
TscAI CASTG 4 cut(s) 70, 101, 281, 415
TspDTI ATGAA 1 cut(s) 152
TspRI CASTG 4 cut(s) 70, 101, 281, 415
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 5 cut(s) 34, 71, 116, 304, 423
XceI RCATGY 1 cut(s) 379
XspI CTAG 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.