Rorug05G0433900

Belongs to the ubiquitin-conjugating enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012716
Physical Location & Seq
Reverse (-)
60284451 .. 60285059
609 bp
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UTR
Exon/CDS
Intron
Rorug05G0433900.1

Sequence Viewer

Length: 609 bp
ATGGAAGAGGTAAAGTTGATATGTCTTGATGAGCGTTGGGTCTCCATTGCAATAGACAATCGAGACGATCTTGGGGATGTTGGGTCCCTCGACTTCAAGTTTCTGGAGCAGGTCTTACCACACTGCTCCAAAGACCAGTTGGTTCACATCGAGAAGAGCACCAAAGGTAAAGATCTGAGTCCCATCACCGATAAGCTGTGGAACAAGTTCTTCGAGAGAGATTTCGGTGTTAAAGCCACAGATGAGGTGATCGAGAAGATGAAGATCAAGAAAGTGAGTTTCAAATGGTCGGAATTGTACCACGCCAAGTTGAAGAGAATGGAAGAGGCTGAGAAAGAAGCGGGTGAAAGGTTGAAGAATCTGTATCAGAAAGAAAGCGCCCGGAAACGAAGCCGGCAAGTTAGGGTTTTGGATAAGGTTCCACCTTCTTCTTCAAGCAATAAAAGAAGTGGATCCAACGCAGGGAGCAAACTGATGAAGAAAGTGAGGAAAGAGTATCTGAATTGTTTGGAGGTGAAAAATCTTGAAGCTGTGAAGATGAAGAGAACTGCCAAGTGTTCTGGTCTCATCAAAAAGCCAAGAACGACTCTTCAAACTAACGTCTTTTGA

Protein Analysis

202

Amino Acids

23.39

Weight (kDa)

9.64

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Elongin_A PF06881 25 - 133 1.2e-23 RNA polymerase II transcription factor SIII (Elongin) subunit A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56150
fragaria_vesca FvH4_3g41701
malus_domestica MD03G1041600.v1.1
prunus_persica Prupe.6G033700_v2.0.a1 Prupe.6G033700_v2.0.a1
pyrus_communis pycom03g03230 pycom11g03660
rosa_chinensis RchiOBHm_Chr5g0074801
rosa_multiflora Rmu_sc0000909.1_g000007 Rmu_sc0005365.1_g000005
rosa_roxburghii Rroxscaffold_1G00006770
rosa_rugosa Rorug05G0433800 Rorug05G0433900
rosa_samantha Rh5AG491800 Rh5BG513200 Rh5CG536900 Rh5DG525300
rosa_wichuraiana Rw5G045550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 100
AciI CCGC 1 cut(s) 341
AclWI GGATC 2 cut(s) 447, 460
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 462
AgsI TTSAA 7 cut(s) 97, 283, 313, 355, 435, 527, 593
AluBI AGCT 2 cut(s) 196, 530
AluI AGCT 2 cut(s) 196, 530
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 3 cut(s) 46, 57, 569
AlwI GGATC 2 cut(s) 447, 460
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 84
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 4 cut(s) 178, 259, 356, 526
AvaII GGWCC 1 cut(s) 84
BamHI GGATCC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 161
BccI CCATC 1 cut(s) 191
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 3 cut(s) 46, 57, 569
BfoI RGCGCY 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 100
BglII AGATCT 1 cut(s) 172
Bme1390I CCNGG 1 cut(s) 382
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 4 cut(s) 85, 86, 420, 454
BmrFI CCNGG 1 cut(s) 382
BpmI CTGGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 263
BsaI GGTCTC 2 cut(s) 46, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse118I RCCGGY 1 cut(s) 393
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 263
BseGI GGATG 1 cut(s) 82
BseJI GATNNNNATC 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 462
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 167, 321
BseNI ACTGG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 2 cut(s) 382, 394
BslFI GGGAC 2 cut(s) 70, 165
BslI CCNNNNNNNGG 1 cut(s) 462
BsmAI GTCTC 3 cut(s) 46, 57, 569
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 2 cut(s) 70, 165
Bso31I GGTCTC 2 cut(s) 46, 569
Bsp1286I GDGCHC 1 cut(s) 161
Bsp143I GATC 5 cut(s) 67, 172, 249, 264, 452
BspACI CCGC 1 cut(s) 341
BspCNI CTCAG 2 cut(s) 168, 322
BspLI GGNNCC 4 cut(s) 85, 86, 420, 454
BspMI ACCTGC 1 cut(s) 100
BspPI GGATC 2 cut(s) 447, 460
BspQI GCTCTTC 1 cut(s) 149
BspTNI GGTCTC 2 cut(s) 46, 569
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 393
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 393
BssMI GATC 5 cut(s) 67, 172, 249, 264, 452
Bst6I CTCTTC 5 cut(s) 149, 308, 318, 536, 594
BstC8I GCNNGC 1 cut(s) 395
BstDEI CTNAG 2 cut(s) 176, 330
BstF5I GGATG 1 cut(s) 82
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 5 cut(s) 70, 175, 252, 267, 455
BstMAI GTCTC 3 cut(s) 46, 57, 569
BstMBI GATC 5 cut(s) 67, 172, 249, 264, 452
BstSCI CCNGG 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 172, 452
BstYI RGATCY 2 cut(s) 172, 452
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 1 cut(s) 395
CfoI GCGC 1 cut(s) 380
Cfr10I RCCGGY 1 cut(s) 393
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 1 cut(s) 298
CviJI RGCY 6 cut(s) 196, 236, 329, 393, 530, 577
CviKI_1 RGCY 6 cut(s) 196, 236, 329, 393, 530, 577
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 2 cut(s) 176, 330
DpnI GATC 5 cut(s) 69, 174, 251, 266, 454
DpnII GATC 5 cut(s) 67, 172, 249, 264, 452
Eam1104I CTCTTC 5 cut(s) 149, 308, 318, 536, 594
EarI CTCTTC 5 cut(s) 149, 308, 318, 536, 594
Eco31I GGTCTC 2 cut(s) 46, 569
Eco47I GGWCC 1 cut(s) 84
EcoO109I RGGNCCY 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 57
FaiI YATR 1 cut(s) 22
FaqI GGGAC 2 cut(s) 70, 165
FauI CCCGC 1 cut(s) 334
FokI GGATG 1 cut(s) 89
GlaI GCGC 1 cut(s) 379
GsuI CTGGAG 1 cut(s) 125
HaeII RGCGCY 1 cut(s) 381
HapII CCGG 2 cut(s) 382, 394
HhaI GCGC 1 cut(s) 380
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 3 cut(s) 178, 358, 586
HpaII CCGG 2 cut(s) 382, 394
HphI GGTGA 4 cut(s) 178, 259, 356, 526
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 4 cut(s) 177, 292, 369, 501
Hpy188III TCNNGA 8 cut(s) 26, 62, 104, 151, 214, 253, 268, 524
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 435
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 1 cut(s) 50
HpyF3I CTNAG 2 cut(s) 176, 330
HpySE526I ACGT 1 cut(s) 600
HspAI GCGC 1 cut(s) 378
KflI GGGWCCC 1 cut(s) 84
KroI GCCGGC 1 cut(s) 393
KroNI GCCGGC 1 cut(s) 395
Kzo9I GATC 5 cut(s) 67, 172, 249, 264, 452
LguI GCTCTTC 1 cut(s) 149
LmnI GCTCC 3 cut(s) 106, 131, 465
LpnPI CCDG 7 cut(s) 89, 95, 149, 395, 407, 447, 546
MaeII ACGT 1 cut(s) 600
MalI GATC 5 cut(s) 69, 174, 251, 266, 454
MboI GATC 5 cut(s) 67, 172, 249, 264, 452
MflI RGATCY 2 cut(s) 172, 452
MhlI GDGCHC 1 cut(s) 161
MluCI AATT 2 cut(s) 293, 502
MlyI GAGTC 2 cut(s) 187, 580
MmeI TCCRAC 2 cut(s) 270, 480
MnlI CCTC 5 cut(s) 98, 238, 319, 480, 505
MroNI GCCGGC 1 cut(s) 393
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 1 cut(s) 231
MspI CCGG 2 cut(s) 382, 394
MspR9I CCNGG 1 cut(s) 382
NaeI GCCGGC 1 cut(s) 395
NciI CCSGG 1 cut(s) 382
NdeII GATC 5 cut(s) 67, 172, 249, 264, 452
NgoMIV GCCGGC 1 cut(s) 393
NlaIV GGNNCC 4 cut(s) 85, 86, 420, 454
PciSI GCTCTTC 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 395
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 358
PleI GAGTC 2 cut(s) 186, 580
PpsI GAGTC 2 cut(s) 186, 580
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
PspN4I GGNNCC 4 cut(s) 85, 86, 420, 454
PspPI GGNCC 1 cut(s) 84
PspPPI RGGWCCY 1 cut(s) 84
PsuI RGATCY 2 cut(s) 172, 452
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SapI GCTCTTC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 231
Sau3AI GATC 5 cut(s) 67, 172, 249, 264, 452
Sau96I GGNCC 1 cut(s) 84
SchI GAGTC 2 cut(s) 187, 580
ScrFI CCNGG 1 cut(s) 382
SduI GDGCHC 1 cut(s) 161
SinI GGWCC 1 cut(s) 84
Sse9I AATT 2 cut(s) 293, 502
SsiI CCGC 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 380
TaiI ACGT 1 cut(s) 603
TaqI TCGA 5 cut(s) 61, 90, 150, 213, 252
TasI AATT 2 cut(s) 293, 502
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TscAI CASTG 1 cut(s) 128
TspDTI ATGAA 3 cut(s) 275, 491, 554
TspRI CASTG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 84
XcmI CCANNNNNNNNNTGG 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.