AT5G56650

IAA-amino acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010736
Physical Location & Seq
Forward (+)
22930775 .. 22932822
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56650.1

Sequence Viewer

Length: 1317 bp
ATGGCTCTAAACAACTTTTTGACTTTCCAACTACTCCTTCTTCTACTTCGTGTTTCATCGGAATCTCCATGGATCGTCGCCGGAGATGTGTCTCGAATCCCGATCAATTTCCTCGAATTAGCAAAGAGCCCGGAGGTTTTCGATTCAATGGTGAGAATCAGAAGGAAAATCCACGAGAATCCTGAGCTGGGATACGAGGAATTCGAAACTAGTAAGTTCATTAGGTCGGAGCTTGACCTTATCGGAGTTAAGTATAGGTTTCCGGTAGCTATCACCGGTATTATCGGATATATCGGTACCGGAGAACCACCATTCGTCGCTCTAAGAGCAGATATGGATGCTTTGCCTATTCAGGAAGCTGTTGAGTGGGAGCACAAGAGCAAAAATCCTGGAAAAATGCATGCTTGTGGACATGATGGTCATGTTGCTATGCTTCTTGGTGCTGCTAAGATACTTCAACAACATCGCCAACATTTGCAGGGAACTGTGGTGCTTATCTTTCAGCCAGCTGAGGAAGGTTTGAGTGGAGCAAAGATGATGAGAGAAGAAGGAGCGTTGAAGAACGTTGAAGCTATTTTTGGTATACATCTTTCCCCCCGGACTCCCTTTGGGAAAGCTGCTTCACTTGCAGGTTCATTTATGGCTGGAGCTGGAGCTTTCGAGGCTGTAATCACTGGGAAAGGAGGTCATGCTGCCATCCCTCAGCATACAATAGATCCTGTTGTTGCAGCTTCAAGCATTGTCCTCAGTCTTCAACACTTGGTTTCACGTGAAACTGACCCTTCGGATTCAAAGGTGGTCACAGTTACTAAGGTTAATGGAGGCAACGCGTTCAATGTCATCCCTGATTCAATCACCATAGGAGGAACTCTTCGAGCCTTTACCGGGTTTACTCAGCTTCAGGAGAGAATCAAAGAGATTATTACAAAGCAAGCAGCCGTTCACCGATGCAATGCGTCCGTGAATCTAGCACCCAATGGTAACCAACCGATGCCACCAACTGTGAACAACATGGACTTATACAAGAAATTCAAGAAGGTGGTTAGGGATTTGTTGGGTCAAGAAGCTTTTGTGGAAGCAGTGCCTGAAATGGGATCCGAGGACTTCTCATACTTTGCAGAAACAATCCCGGGCCATTTCTCCCTCTTGGGAATGCAAGATGAGACCCAAGGTTATGCGTCGTCTCATTCACCACACTATAGAATCAACGAGGATGTTCTTCCTTACGGAGCTGCTATCCATGCAACGATGGCAGTACAGTACCTCAAAGATAAGGCTTCTAAAGGTTCTGTCTCAGGCTTTCATGACGAACTCTAA

Protein Analysis

438

Amino Acids

47.69

Weight (kDa)

6.19

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 107 - 418 1.2e-43 Peptidase family M20/M25/M40
M20_dimer PF07687 216 - 312 2.6e-10 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56650 AT5G56650 AT5G56660
fragaria_vesca FvH4_1g06730 FvH4_1g06730
malus_domestica MD02G1072200.v1.1 MD15G1202000.v1.1
prunus_persica Prupe.7G215300_v2.0.a1
pyrus_communis pycom02g05620 pycom15g17930
rosa_chinensis RchiOBHm_Chr2g0092471
rosa_laevigata RLG00000016315
rosa_multiflora Rmu_sc0004296.1_g000017 Rmu_sc0023787.1_g000002
rosa_roxburghii Rroxscaffold_2G00149070
rosa_rugosa Rorug02G0026900
rosa_samantha Rh2AG072700 Rh2BG073200 Rh2CG074500
rosa_wichuraiana Rw2G006020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 417
Acc36I ACCTGC 1 cut(s) 620
Acc65I GGTACC 1 cut(s) 296
AccB1I GGYRCC 1 cut(s) 296
AccI GTMKAC 1 cut(s) 583
AccII CGCG 1 cut(s) 830
AclI AACGTT 1 cut(s) 564
AclWI GGATC 4 cut(s) 80, 710, 1089, 1102
AcsI RAATTY 2 cut(s) 200, 1028
AcuI CTGAAG 1 cut(s) 884
AcvI CACGTG 1 cut(s) 770
AfaI GTAC 3 cut(s) 298, 1257, 1262
AfiI CCNNNNNNNGG 3 cut(s) 188, 885, 1091
AflIII ACRYGT 1 cut(s) 828
AgeI ACCGGT 1 cut(s) 275
AhlI ACTAGT 1 cut(s) 209
AjnI CCWGG 1 cut(s) 388
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 561, 593
Alw21I GWGCWC 1 cut(s) 375
Alw26I GTCTC 4 cut(s) 96, 1157, 1188, 1297
AlwI GGATC 4 cut(s) 80, 710, 1089, 1102
AlwNI CAGNNNCTG 1 cut(s) 1085
Ama87I CYCGRG 1 cut(s) 1129
AoxI GGCC 1 cut(s) 1132
ApeKI GCWGC 6 cut(s) 443, 617, 692, 728, 935, 1232
ApoI RAATTY 2 cut(s) 200, 1028
AsiGI ACCGGT 1 cut(s) 275
Asp718I GGTACC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 1132
AsuC2I CCSGG 5 cut(s) 131, 598, 886, 1130, 1131
AsuHPI GGTGA 5 cut(s) 163, 265, 847, 935, 1182
AsuII TTCGAA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 1129
BamHI GGATCC 1 cut(s) 1094
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 131
BauI CACGAG 1 cut(s) 173
BbrPI CACGTG 1 cut(s) 770
BbsI GAAGAC 1 cut(s) 743
Bbv12I GWGCWC 1 cut(s) 375
BbvCI CCTCAGC 2 cut(s) 510, 702
BbvI GCAGC 6 cut(s) 430, 604, 679, 740, 947, 1219
BccI CCATC 3 cut(s) 410, 704, 1243
BceAI ACGGC 1 cut(s) 923
BciT130I CCWGG 1 cut(s) 390
BciVI GTATCC 1 cut(s) 185
BcnI CCSGG 5 cut(s) 131, 598, 886, 1130, 1131
BcoDI GTCTC 4 cut(s) 96, 1157, 1188, 1297
BcuI ACTAGT 1 cut(s) 209
BfaI CTAG 2 cut(s) 210, 968
BfmI CTRYAG 1 cut(s) 1198
BfuAI ACCTGC 1 cut(s) 620
BfuI GTATCC 1 cut(s) 185
BisI GCNGC 6 cut(s) 444, 618, 693, 729, 936, 1233
BlsI GCNGC 6 cut(s) 445, 619, 694, 730, 937, 1234
Bme1390I CCNGG 6 cut(s) 131, 390, 598, 886, 1130, 1131
BmeT110I CYCGRG 1 cut(s) 1129
BmgT120I GGNCC 1 cut(s) 1132
BmiI GGNNCC 2 cut(s) 298, 1096
BmrFI CCNGG 6 cut(s) 131, 390, 598, 886, 1130, 1131
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 3 cut(s) 328, 938, 981
BmuI ACTGGG 1 cut(s) 684
BpiI GAAGAC 1 cut(s) 743
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
BpmI CTGGAG 2 cut(s) 666, 672
Bpu10I CCTNAGC 3 cut(s) 183, 510, 702
Bpu14I TTCGAA 1 cut(s) 204
BpuMI CCSGG 5 cut(s) 131, 598, 886, 1130, 1131
BsaAI YACGTR 1 cut(s) 770
BsaI GGTCTC 1 cut(s) 1157
BsaJI CCNNGG 5 cut(s) 68, 596, 1098, 1129, 1168
BsaWI WCCGGW 3 cut(s) 262, 275, 299
Bsc4I CCNNNNNNNGG 3 cut(s) 188, 885, 1091
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 1 cut(s) 958
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 5 cut(s) 68, 596, 1098, 1129, 1168
BseGI GGATG 4 cut(s) 343, 696, 840, 1219
BseLI CCNNNNNNNGG 3 cut(s) 188, 885, 1091
BseMI GCAATG 1 cut(s) 958
BseMII CTCAG 6 cut(s) 174, 501, 716, 760, 908, 1308
BseNI ACTGG 1 cut(s) 679
BseXI GCAGC 6 cut(s) 430, 604, 679, 740, 947, 1219
BseYI CCCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 830
BshFI GGCC 1 cut(s) 1134
BshNI GGYRCC 1 cut(s) 296
BshTI ACCGGT 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 375
BsiHKCI CYCGRG 1 cut(s) 1129
BsiSI CCGG 8 cut(s) 81, 131, 263, 276, 300, 598, 885, 1130
BslI CCNNNNNNNGG 3 cut(s) 188, 885, 1091
BsmAI GTCTC 4 cut(s) 96, 1157, 1188, 1297
BsmBI CGTCTC 1 cut(s) 1188
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 1 cut(s) 1134
Bso31I GGTCTC 1 cut(s) 1157
BsoBI CYCGRG 1 cut(s) 1129
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 131, 375
Bsp143I GATC 4 cut(s) 72, 102, 715, 1094
Bsp19I CCATGG 1 cut(s) 68
BspANI GGCC 1 cut(s) 1134
BspCNI CTCAG 6 cut(s) 175, 502, 715, 759, 907, 1307
BspFNI CGCG 1 cut(s) 830
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 2 cut(s) 298, 1096
BspMI ACCTGC 1 cut(s) 620
BspPI GGATC 4 cut(s) 80, 710, 1089, 1102
BspT104I TTCGAA 1 cut(s) 204
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 1157
BsrDI GCAATG 1 cut(s) 958
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 679
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 5 cut(s) 68, 596, 1098, 1129, 1168
BssMI GATC 4 cut(s) 72, 102, 715, 1094
BssNAI GTATAC 1 cut(s) 584
BssSI CACGAG 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 68, 1168
Bst1107I GTATAC 1 cut(s) 584
Bst2BI CACGAG 1 cut(s) 173
Bst2UI CCWGG 1 cut(s) 390
Bst4CI ACNGT 4 cut(s) 487, 805, 1003, 1260
Bst6I CTCTTC 1 cut(s) 876
BstBAI YACGTR 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 402, 507, 933
BstDEI CTNAG 9 cut(s) 183, 323, 447, 510, 702, 746, 810, 894, 1294
BstDSI CCRYGG 1 cut(s) 68
BstEII GGTNACC 1 cut(s) 980
BstF5I GGATG 4 cut(s) 343, 696, 840, 1219
BstFNI CGCG 1 cut(s) 830
BstKTI GATC 4 cut(s) 75, 105, 718, 1097
BstMAI GTCTC 4 cut(s) 96, 1157, 1188, 1297
BstMBI GATC 4 cut(s) 72, 102, 715, 1094
BstMWI GCNNNNNNNGC 5 cut(s) 326, 626, 662, 1241, 1250
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 404
BstPI GGTNACC 1 cut(s) 980
BstSCI CCNGG 6 cut(s) 129, 388, 596, 884, 1128, 1129
BstSFI CTRYAG 1 cut(s) 1198
BstUI CGCG 1 cut(s) 830
BstV1I GCAGC 6 cut(s) 430, 604, 679, 740, 947, 1219
BstV2I GAAGAC 1 cut(s) 743
BstX2I RGATCY 2 cut(s) 715, 1094
BstYI RGATCY 2 cut(s) 715, 1094
BstZ17I GTATAC 1 cut(s) 584
BsuI GTATCC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 1134
BtgI CCRYGG 1 cut(s) 68
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 4 cut(s) 343, 696, 840, 1219
BtsI GCAGTG 1 cut(s) 1086
BtsIMutI CAGTG 2 cut(s) 672, 1086
BveI ACCTGC 1 cut(s) 620
Cac8I GCNNGC 3 cut(s) 402, 507, 933
CaiI CAGNNNCTG 1 cut(s) 1085
CciI TCATGA 1 cut(s) 1303
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 1132
Cfr9I CCCGGG 1 cut(s) 1129
CseI GACGC 2 cut(s) 945, 1167
Csp6I GTAC 3 cut(s) 297, 1256, 1261
CspAI ACCGGT 1 cut(s) 275
CviAII CATG 8 cut(s) 69, 401, 413, 422, 689, 1012, 1241, 1304
CviQI GTAC 3 cut(s) 297, 1256, 1261
DdeI CTNAG 9 cut(s) 183, 323, 447, 510, 702, 746, 810, 894, 1294
DpnI GATC 4 cut(s) 74, 104, 717, 1096
DpnII GATC 4 cut(s) 72, 102, 715, 1094
DrdI GACNNNNNNGTC 1 cut(s) 417
DseDI GACNNNNNNGTC 1 cut(s) 417
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco130I CCWWGG 2 cut(s) 68, 1168
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 884
Eco72I CACGTG 1 cut(s) 770
Eco88I CYCGRG 1 cut(s) 1129
Eco91I GGTNACC 1 cut(s) 980
EcoO65I GGTNACC 1 cut(s) 980
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 68, 1168
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 2 cut(s) 68, 1168
Esp3I CGTCTC 1 cut(s) 1188
FaeI CATG 8 cut(s) 72, 404, 416, 425, 692, 1015, 1244, 1307
FatI CATG 8 cut(s) 68, 400, 412, 421, 688, 1011, 1240, 1303
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 6 cut(s) 444, 618, 693, 729, 936, 1233
FokI GGATG 4 cut(s) 350, 683, 827, 1226
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 6 cut(s) 444, 618, 693, 729, 936, 1233
FspBI CTAG 2 cut(s) 210, 968
GluI GCNGC 6 cut(s) 444, 618, 693, 729, 936, 1233
GsaI CCCAGC 1 cut(s) 191
GsuI CTGGAG 2 cut(s) 666, 672
HaeIII GGCC 1 cut(s) 1134
HapII CCGG 8 cut(s) 81, 131, 263, 276, 300, 598, 885, 1130
HgaI GACGC 2 cut(s) 945, 1167
Hin1II CATG 8 cut(s) 72, 404, 416, 425, 692, 1015, 1244, 1307
HindIII AAGCTT 1 cut(s) 1065
HpaII CCGG 8 cut(s) 81, 131, 263, 276, 300, 598, 885, 1130
HphI GGTGA 5 cut(s) 163, 265, 847, 935, 1182
Hpy166II GTNNAC 5 cut(s) 410, 584, 891, 943, 1006
Hpy188I TCNGA 7 cut(s) 61, 161, 229, 245, 287, 787, 1099
Hpy188III TCNNGA 8 cut(s) 93, 100, 182, 353, 902, 1033, 1061, 1304
Hpy8I GTNNAC 5 cut(s) 410, 584, 891, 943, 1006
Hpy99I CGWCG 3 cut(s) 80, 320, 1183
HpyAV CCTTC 6 cut(s) 47, 156, 509, 542, 792, 1030
HpyCH4III ACNGT 4 cut(s) 487, 805, 1003, 1260
HpyCH4IV ACGT 2 cut(s) 564, 769
HpyCH4V TGCA 8 cut(s) 400, 478, 629, 728, 951, 1118, 1156, 1244
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 626, 662, 1241, 1250
HpyF3I CTNAG 9 cut(s) 183, 323, 447, 510, 702, 746, 810, 894, 1294
HpySE526I ACGT 2 cut(s) 564, 769
Hsp92II CATG 8 cut(s) 72, 404, 416, 425, 692, 1015, 1244, 1307
KpnI GGTACC 1 cut(s) 300
Kzo9I GATC 4 cut(s) 72, 102, 715, 1094
LmnI GCTCC 7 cut(s) 229, 370, 527, 551, 647, 653, 1229
Lsp1109I GCAGC 6 cut(s) 430, 604, 679, 740, 947, 1219
LweI GCATC 3 cut(s) 328, 938, 981
MaeI CTAG 2 cut(s) 210, 968
MaeII ACGT 2 cut(s) 564, 769
MaeIII GTNAC 3 cut(s) 799, 805, 980
MalI GATC 4 cut(s) 74, 104, 717, 1096
MboI GATC 4 cut(s) 72, 102, 715, 1094
MboII GAAGA 6 cut(s) 32, 557, 571, 743, 863, 1211
MflI RGATCY 2 cut(s) 715, 1094
MhlI GDGCHC 2 cut(s) 131, 375
MluCI AATT 4 cut(s) 106, 116, 200, 1028
MluI ACGCGT 1 cut(s) 828
MlyI GAGTC 1 cut(s) 595
MmeI TCCRAC 2 cut(s) 52, 207
Mph1103I ATGCAT 1 cut(s) 402
MseI TTAA 2 cut(s) 249, 816
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 8 cut(s) 81, 131, 263, 276, 300, 598, 885, 1130
MspR9I CCNGG 6 cut(s) 131, 390, 598, 886, 1130, 1131
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 1 cut(s) 390
MvnI CGCG 1 cut(s) 830
MwoI GCNNNNNNNGC 5 cut(s) 326, 626, 662, 1241, 1250
NciI CCSGG 5 cut(s) 131, 598, 886, 1130, 1131
NcoI CCATGG 1 cut(s) 68
NdeII GATC 4 cut(s) 72, 102, 715, 1094
NlaIII CATG 8 cut(s) 72, 404, 416, 425, 692, 1015, 1244, 1307
NlaIV GGNNCC 2 cut(s) 298, 1096
NmuCI GTSAC 1 cut(s) 799
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 404
NspV TTCGAA 1 cut(s) 204
PaeI GCATGC 1 cut(s) 404
PagI TCATGA 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 1158
PfoI TCCNGGA 1 cut(s) 388
PinAI ACCGGT 1 cut(s) 275
PkrI GCNGC 6 cut(s) 445, 619, 694, 730, 937, 1234
PleI GAGTC 1 cut(s) 595
PmaCI CACGTG 1 cut(s) 770
PmlI CACGTG 1 cut(s) 770
PpsI GAGTC 1 cut(s) 595
Ppu21I YACGTR 1 cut(s) 770
Psp1406I AACGTT 1 cut(s) 564
Psp6I CCWGG 1 cut(s) 388
PspCI CACGTG 1 cut(s) 770
PspEI GGTNACC 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 298, 1096
PspPI GGNCC 1 cut(s) 1132
PstNI CAGNNNCTG 1 cut(s) 1085
PsuI RGATCY 2 cut(s) 715, 1094
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 3 cut(s) 298, 1257, 1262
RsaNI GTAC 3 cut(s) 297, 1256, 1261
RseI CAYNNNNRTG 1 cut(s) 405
SaqAI TTAA 2 cut(s) 249, 816
SatI GCNGC 6 cut(s) 444, 618, 693, 729, 936, 1233
Sau3AI GATC 4 cut(s) 72, 102, 715, 1094
Sau96I GGNCC 1 cut(s) 1132
SchI GAGTC 1 cut(s) 595
ScrFI CCNGG 6 cut(s) 131, 390, 598, 886, 1130, 1131
SduI GDGCHC 2 cut(s) 131, 375
SfaNI GCATC 3 cut(s) 328, 938, 981
SfcI CTRYAG 1 cut(s) 1198
SfuI TTCGAA 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 1131
SmiMI CAYNNNNRTG 1 cut(s) 405
SpeI ACTAGT 1 cut(s) 209
SphI GCATGC 1 cut(s) 404
Sse9I AATT 4 cut(s) 106, 116, 200, 1028
SspMI CTAG 2 cut(s) 210, 968
StyD4I CCNGG 6 cut(s) 129, 388, 596, 884, 1128, 1129
StyI CCWWGG 2 cut(s) 68, 1168
TaaI ACNGT 4 cut(s) 487, 805, 1003, 1260
TaiI ACGT 2 cut(s) 567, 772
TaqI TCGA 6 cut(s) 94, 114, 141, 204, 660, 874
TasI AATT 4 cut(s) 106, 116, 200, 1028
TatI WGTACW 1 cut(s) 1255
Tru1I TTAA 2 cut(s) 249, 816
Tru9I TTAA 2 cut(s) 249, 816
TscAI CASTG 2 cut(s) 679, 1086
TseFI GTSAC 1 cut(s) 799
TseI GCWGC 6 cut(s) 443, 617, 692, 728, 935, 1232
Tsp45I GTSAC 1 cut(s) 799
TspDTI ATGAA 4 cut(s) 45, 208, 624, 1292
TspGWI ACGGA 2 cut(s) 949, 1242
TspMI CCCGGG 1 cut(s) 1129
TspRI CASTG 2 cut(s) 679, 1086
XapI RAATTY 2 cut(s) 200, 1028
XceI RCATGY 1 cut(s) 404
XmaI CCCGGG 1 cut(s) 1129
XmiI GTMKAC 1 cut(s) 583
XspI CTAG 2 cut(s) 210, 968
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.