FvH4_1g06730

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010736
Physical Location & Seq
Forward (+)
3545285 .. 3548402
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06730.t1

Sequence Viewer

Length: 1422 bp
ATGATTATATTCAACCCTCACCAAATGTTCTCCAGTTCAATCATTTCATCAATGGCTTCCCCCAAATGGGTCTCTTTGACTTTCATACTCTCTATGTTTATGCCACTACCCATTTCCTCAAACCAATCTTTGAGCCCAGAACCGGAGCTGGCTCAAATCCCAGTCTCGTTTCTTGAACACGCTAAAAGACCCGAGCTTTTTGATCGTATGGTGAGTATCAGAAGGAAAATCCATGAAAACCCAGAGCTTTGTTACGAGGAATTTGAAACCAGTAAGCTTATCAGAGCTGAACTTGACCGGCTGGGCATCCCTTACAGATACCCTTTGGCTGAAACCGGCGTTGTTGGCGTTGTTGGGACTGGTGGTCCTCCCTTTGTTGCAATCAGAGCAGACATGGATGCTCTGGCTATGCAGGAGAGTGTGGAGTGGGAGCACAAGAGTAAATTTGCAGGGAAGATGCATGGATGTGGACATGATGGTCATGTTGCCATGCTTCTTGGAGCTGCCAGTTTACTTCAAGAGTATCGCCACGTATTACAGGGGACTGTTGTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGTGCAGGAGCAAAGAAAATGATAGAAAACGGGGCACTGGAGCATGTCAGTGCCATCTTTGGGTTGCATCTTGCTTCTGACCACCCCATTGGTTCAGTAGCCTCCAGGCCTGGCCCTCTATTGGCTGCGAGTGGATTTTTTGAAGCCTTGATAAGTGGGAAGGGGGGTCATGCTGCAATTCCACAGCACACAATAGATCCTATCTTGGCTGCTTCCAATGTTATTGTTAGTCTGCAACATCTTGTTTCACGTGAAGCTGACCCCTTAGATTCACAGGTTGTGACTGTTGGAAAATTCCAAGGAGGTGGTGCTTTTAATGTTATTCCAGATTCTGTTACAATTGGGGGTACCTTCAGGGCATTTTCAAAGGACAGCTTTTTTCAACTTAAAGAAAGGATCGAAGAGGTTATTACTAGACAAGCCAGTGTTCAGAGGTGCAATGCAACTGTCTTGTTCAATGAAATTGCAAAACCCTTCTACCCTGCAACTATAAATGACAAAAATTTGCATGAGCACTTCCAGAAAGTTGCAGGGGATTTGCTGGGTTCTCAGAATGTTCTTGAGAAGAAACCACTTATGGGGGCAGAAGACTTCTCATTCTATCAAGAAGTAATTCCAGGGTACTACTTCTTTCTTGGTATGAAGAATGACTCTCAAGGACAGTTCAAACCAATGCATTCACCATATTTCAGAATAAACGAAGACGTGCTCCCATATGGAGCTGCATTCCATGCATCACTGGCCACCAGGTACCTCCTGGAAAACCAACCGAAATCTACTTCAACGTCAGACCAAAAAACAGAATCTACTTCAACGAAAGGGAACTTCCGTGATGAACTGTGA

Protein Analysis

474

Amino Acids

51.95

Weight (kDa)

5.98

Isoelectric Point (pI)

37.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 127 - 442 1.5e-43 Peptidase family M20/M25/M40
M20_dimer PF07687 244 - 333 2e-10 Peptidase dimerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56650 AT5G56650 AT5G56660
fragaria_vesca FvH4_1g06730 FvH4_1g06730
malus_domestica MD02G1072200.v1.1 MD15G1202000.v1.1
prunus_persica Prupe.7G215300_v2.0.a1
pyrus_communis pycom02g05620 pycom15g17930
rosa_chinensis RchiOBHm_Chr2g0092471
rosa_laevigata RLG00000016315
rosa_multiflora Rmu_sc0004296.1_g000017 Rmu_sc0023787.1_g000002
rosa_roxburghii Rroxscaffold_2G00149070
rosa_rugosa Rorug02G0026900
rosa_samantha Rh2AG072700 Rh2BG073200 Rh2CG074500
rosa_wichuraiana Rw2G006020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 477
Acc65I GGTACC 2 cut(s) 926, 1329
AccB1I GGYRCC 2 cut(s) 926, 1329
AclWI GGATC 2 cut(s) 770, 983
AcoI YGGCCR 1 cut(s) 1320
AcsI RAATTY 4 cut(s) 260, 443, 872, 1081
AcuI CTGAAG 1 cut(s) 916
AcvI CACGTG 1 cut(s) 830
AfaI GTAC 3 cut(s) 928, 1202, 1331
AfiI CCNNNNNNNGG 6 cut(s) 66, 67, 142, 639, 700, 1157
AhdI GACNNNNNGTC 1 cut(s) 363
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 5 cut(s) 683, 688, 1195, 1325, 1335
Alw21I GWGCWC 3 cut(s) 435, 1095, 1290
Alw26I GTCTC 2 cut(s) 76, 169
AlwI GGATC 2 cut(s) 770, 983
AlwNI CAGNNNCTG 1 cut(s) 911
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 3 cut(s) 686, 691, 1320
ApeKI GCWGC 5 cut(s) 503, 704, 752, 788, 1301
ApoI RAATTY 4 cut(s) 260, 443, 872, 1081
Asp700I GAANNNNTTC 1 cut(s) 1191
Asp718I GGTACC 2 cut(s) 926, 1329
AspS9I GGNCC 2 cut(s) 365, 692
AsuHPI GGTGA 3 cut(s) 11, 223, 1251
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 365
BaeGI GKGCMC 1 cut(s) 616
BalI TGGCCA 1 cut(s) 1322
BanI GGYRCC 2 cut(s) 926, 1329
BanII GRGCYC 1 cut(s) 137
BbrPI CACGTG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 1173, 1287
Bbv12I GWGCWC 3 cut(s) 435, 1095, 1290
BbvCI CCTCAGC 1 cut(s) 570
BbvI GCAGC 5 cut(s) 490, 691, 739, 775, 1288
BccI CCATC 2 cut(s) 470, 641
BciT130I CCWGG 5 cut(s) 685, 690, 1197, 1327, 1337
BcoDI GTCTC 2 cut(s) 76, 169
BfaI CTAG 1 cut(s) 993
BisI GCNGC 5 cut(s) 504, 705, 753, 789, 1302
BlsI GCNGC 5 cut(s) 505, 706, 754, 790, 1303
Bme1390I CCNGG 5 cut(s) 685, 690, 1197, 1327, 1337
Bme18I GGWCC 1 cut(s) 365
BmeRI GACNNNNNGTC 1 cut(s) 363
BmeT110I CYCGRG 1 cut(s) 191
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 2 cut(s) 365, 692
BmiI GGNNCC 2 cut(s) 928, 1331
BmrFI CCNGG 5 cut(s) 685, 690, 1197, 1327, 1337
BmrI ACTGGG 1 cut(s) 155
BmsI GCATC 5 cut(s) 315, 388, 447, 655, 1322
BmuI ACTGGG 1 cut(s) 155
BpiI GAAGAC 2 cut(s) 1173, 1287
BpmI CTGGAG 3 cut(s) 16, 638, 667
Bpu10I CCTNAGC 1 cut(s) 570
BpuEI CTTGAG 2 cut(s) 1160, 1218
BsaAI YACGTR 2 cut(s) 532, 830
BsaI GGTCTC 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 877, 1196
BsaWI WCCGGW 1 cut(s) 142
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 67, 142, 639, 700, 1157
Bse118I RCCGGY 2 cut(s) 297, 335
Bse1I ACTGG 8 cut(s) 33, 161, 270, 364, 507, 621, 1002, 1323
Bse3DI GCAATG 1 cut(s) 1024
BseBI CCWGG 5 cut(s) 685, 690, 1197, 1327, 1337
BseDI CCNNGG 2 cut(s) 877, 1196
BseGI GGATG 3 cut(s) 306, 403, 470
BseLI CCNNNNNNNGG 6 cut(s) 66, 67, 142, 639, 700, 1157
BseMI GCAATG 1 cut(s) 1024
BseMII CTCAG 2 cut(s) 561, 1142
BseNI ACTGG 8 cut(s) 33, 161, 270, 364, 507, 621, 1002, 1323
BseSI GKGCMC 1 cut(s) 616
BseXI GCAGC 5 cut(s) 490, 691, 739, 775, 1288
BseYI CCCAGC 2 cut(s) 301, 1120
BsgI GTGCAG 1 cut(s) 603
BshFI GGCC 3 cut(s) 688, 693, 1322
BshNI GGYRCC 2 cut(s) 926, 1329
BsiHKAI GWGCWC 3 cut(s) 435, 1095, 1290
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 3 cut(s) 143, 298, 336
BslFI GGGAC 2 cut(s) 370, 556
BslI CCNNNNNNNGG 6 cut(s) 66, 67, 142, 639, 700, 1157
BsmAI GTCTC 2 cut(s) 76, 169
BsmFI GGGAC 2 cut(s) 370, 556
BsmI GAATGC 2 cut(s) 1255, 1304
BsnI GGCC 3 cut(s) 688, 693, 1322
Bso31I GGTCTC 1 cut(s) 76
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 5 cut(s) 137, 435, 616, 1095, 1290
Bsp143I GATC 3 cut(s) 202, 775, 975
BspANI GGCC 3 cut(s) 688, 693, 1322
BspCNI CTCAG 2 cut(s) 562, 1141
BspLI GGNNCC 2 cut(s) 928, 1331
BspPI GGATC 2 cut(s) 770, 983
BspT107I GGYRCC 2 cut(s) 926, 1329
BspTNI GGTCTC 1 cut(s) 76
BsrDI GCAATG 1 cut(s) 1024
BsrFI RCCGGY 2 cut(s) 297, 335
BsrI ACTGG 8 cut(s) 33, 161, 270, 364, 507, 621, 1002, 1323
BssAI RCCGGY 2 cut(s) 297, 335
BssECI CCNNGG 2 cut(s) 877, 1196
BssMI GATC 3 cut(s) 202, 775, 975
BssT1I CCWWGG 1 cut(s) 877
Bst2UI CCWGG 5 cut(s) 685, 690, 1197, 1327, 1337
Bst4CI ACNGT 5 cut(s) 547, 865, 1027, 1242, 1419
Bst6I CTCTTC 1 cut(s) 975
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstBAI YACGTR 2 cut(s) 532, 830
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 570, 844, 1128
BstF5I GGATG 3 cut(s) 306, 403, 470
BstKTI GATC 3 cut(s) 205, 778, 978
BstMAI GTCTC 2 cut(s) 76, 169
BstMBI GATC 3 cut(s) 202, 775, 975
BstMWI GCNNNNNNNGC 4 cut(s) 345, 386, 1310, 1319
BstNI CCWGG 5 cut(s) 685, 690, 1197, 1327, 1337
BstNSI RCATGY 1 cut(s) 626
BstSCI CCNGG 5 cut(s) 683, 688, 1195, 1325, 1335
BstSLI GKGCMC 1 cut(s) 616
BstV1I GCAGC 5 cut(s) 490, 691, 739, 775, 1288
BstV2I GAAGAC 2 cut(s) 1173, 1287
BstX2I RGATCY 1 cut(s) 775
BstXI CCANNNNNNTGG 2 cut(s) 668, 884
BstYI RGATCY 1 cut(s) 775
BsuRI GGCC 3 cut(s) 688, 693, 1322
BtrI CACGTC 1 cut(s) 1285
BtsCI GGATG 3 cut(s) 306, 403, 470
BtsIMutI CAGTG 4 cut(s) 614, 634, 1009, 1316
Cac8I GCNNGC 1 cut(s) 150
CaiI CAGNNNCTG 1 cut(s) 911
Cfr10I RCCGGY 2 cut(s) 297, 335
Cfr13I GGNCC 2 cut(s) 365, 692
CsiI ACCWGGT 1 cut(s) 1325
Csp6I GTAC 3 cut(s) 927, 1201, 1330
CviQI GTAC 3 cut(s) 927, 1201, 1330
DdeI CTNAG 3 cut(s) 570, 844, 1128
DpnI GATC 3 cut(s) 204, 777, 977
DpnII GATC 3 cut(s) 202, 775, 975
DrdI GACNNNNNNGTC 1 cut(s) 477
DriI GACNNNNNGTC 1 cut(s) 363
DseDI GACNNNNNNGTC 1 cut(s) 477
EaeI YGGCCR 1 cut(s) 1320
Eam1104I CTCTTC 1 cut(s) 975
Eam1105I GACNNNNNGTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 975
Eco130I CCWWGG 1 cut(s) 877
Eco147I AGGCCT 1 cut(s) 688
Eco24I GRGCYC 1 cut(s) 137
Eco31I GGTCTC 1 cut(s) 76
Eco47I GGWCC 1 cut(s) 365
Eco57I CTGAAG 1 cut(s) 916
Eco72I CACGTG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 191
EcoRII CCWGG 5 cut(s) 683, 688, 1195, 1325, 1335
EcoT14I CCWWGG 1 cut(s) 877
EcoT22I ATGCAT 3 cut(s) 462, 1257, 1315
EcoT38I GRGCYC 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 877
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 2 cut(s) 370, 556
FauNDI CATATG 1 cut(s) 1294
Fnu4HI GCNGC 5 cut(s) 504, 705, 753, 789, 1302
FokI GGATG 3 cut(s) 293, 410, 477
FriOI GRGCYC 1 cut(s) 137
Fsp4HI GCNGC 5 cut(s) 504, 705, 753, 789, 1302
FspBI CTAG 1 cut(s) 993
GluI GCNGC 5 cut(s) 504, 705, 753, 789, 1302
GsaI CCCAGC 2 cut(s) 305, 1124
GsuI CTGGAG 3 cut(s) 16, 638, 667
HaeIII GGCC 3 cut(s) 688, 693, 1322
HapII CCGG 3 cut(s) 143, 298, 336
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 4 cut(s) 848, 908, 1229, 1382
HpaII CCGG 3 cut(s) 143, 298, 336
HphI GGTGA 3 cut(s) 11, 223, 1251
Hpy166II GTNNAC 2 cut(s) 470, 512
Hpy188I TCNGA 8 cut(s) 221, 284, 386, 658, 1011, 1131, 1271, 1369
Hpy188III TCNNGA 6 cut(s) 173, 518, 905, 1099, 1139, 1184
Hpy8I GTNNAC 2 cut(s) 470, 512
HpyAV CCTTC 5 cut(s) 216, 569, 733, 940, 1063
HpyCH4III ACNGT 5 cut(s) 547, 865, 1027, 1242, 1419
HpyCH4IV ACGT 4 cut(s) 531, 829, 1284, 1364
HpyF10VI GCNNNNNNNGC 4 cut(s) 345, 386, 1310, 1319
HpyF3I CTNAG 3 cut(s) 570, 844, 1128
HpySE526I ACGT 4 cut(s) 531, 829, 1284, 1364
KpnI GGTACC 2 cut(s) 930, 1333
Kzo9I GATC 3 cut(s) 202, 775, 975
LmnI GCTCC 7 cut(s) 145, 430, 500, 587, 619, 1293, 1298
Lsp1109I GCAGC 5 cut(s) 490, 691, 739, 775, 1288
LweI GCATC 5 cut(s) 315, 388, 447, 655, 1322
MabI ACCWGGT 1 cut(s) 1325
MaeI CTAG 1 cut(s) 993
MaeII ACGT 4 cut(s) 531, 829, 1284, 1364
MaeIII GTNAC 3 cut(s) 251, 859, 913
MalI GATC 3 cut(s) 204, 777, 977
MboI GATC 3 cut(s) 202, 775, 975
MboII GAAGA 6 cut(s) 466, 992, 1156, 1178, 1234, 1292
MfeI CAATTG 1 cut(s) 918
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 5 cut(s) 137, 435, 616, 1095, 1290
MlsI TGGCCA 1 cut(s) 1322
MluCI AATT 8 cut(s) 260, 443, 756, 872, 918, 1041, 1081, 1191
MluNI TGGCCA 1 cut(s) 1322
MlyI GAGTC 1 cut(s) 1223
MmeI TCCRAC 2 cut(s) 586, 847
Mox20I TGGCCA 1 cut(s) 1322
Mph1103I ATGCAT 3 cut(s) 462, 1257, 1315
MroXI GAANNNNTTC 1 cut(s) 1191
MscI TGGCCA 1 cut(s) 1322
MseI TTAA 2 cut(s) 894, 966
MslI CAYNNNNRTG 2 cut(s) 465, 627
Msp20I TGGCCA 1 cut(s) 1322
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 3 cut(s) 143, 298, 336
MspR9I CCNGG 5 cut(s) 685, 690, 1197, 1327, 1337
MunI CAATTG 1 cut(s) 918
Mva1269I GAATGC 2 cut(s) 1255, 1304
MvaI CCWGG 5 cut(s) 685, 690, 1197, 1327, 1337
MwoI GCNNNNNNNGC 4 cut(s) 345, 386, 1310, 1319
NdeI CATATG 1 cut(s) 1294
NdeII GATC 3 cut(s) 202, 775, 975
NlaIV GGNNCC 2 cut(s) 928, 1331
NmuCI GTSAC 1 cut(s) 859
NsiI ATGCAT 3 cut(s) 462, 1257, 1315
NspI RCATGY 1 cut(s) 626
PceI AGGCCT 1 cut(s) 688
PctI GAATGC 2 cut(s) 1255, 1304
PdmI GAANNNNTTC 1 cut(s) 1191
PfeI GAWTC 3 cut(s) 848, 908, 1382
PfoI TCCNGGA 1 cut(s) 1335
PkrI GCNGC 5 cut(s) 505, 706, 754, 790, 1303
PleI GAGTC 1 cut(s) 1223
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
PpsI GAGTC 1 cut(s) 1223
Ppu21I YACGTR 2 cut(s) 532, 830
Psp6I CCWGG 5 cut(s) 683, 688, 1195, 1325, 1335
PspCI CACGTG 1 cut(s) 830
PspFI CCCAGC 2 cut(s) 301, 1120
PspGI CCWGG 5 cut(s) 683, 688, 1195, 1325, 1335
PspN4I GGNNCC 2 cut(s) 928, 1331
PspPI GGNCC 2 cut(s) 365, 692
PstNI CAGNNNCTG 1 cut(s) 911
PsuI RGATCY 1 cut(s) 775
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 3 cut(s) 928, 1202, 1331
RsaNI GTAC 3 cut(s) 927, 1201, 1330
RseI CAYNNNNRTG 2 cut(s) 465, 627
SaqAI TTAA 2 cut(s) 894, 966
SatI GCNGC 5 cut(s) 504, 705, 753, 789, 1302
Sau3AI GATC 3 cut(s) 202, 775, 975
Sau96I GGNCC 2 cut(s) 365, 692
SchI GAGTC 1 cut(s) 1223
ScrFI CCNGG 5 cut(s) 685, 690, 1197, 1327, 1337
SduI GDGCHC 5 cut(s) 137, 435, 616, 1095, 1290
SexAI ACCWGGT 1 cut(s) 1325
SfaNI GCATC 5 cut(s) 315, 388, 447, 655, 1322
SinI GGWCC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 465, 627
SmlI CTYRAG 2 cut(s) 1139, 1233
SmoI CTYRAG 2 cut(s) 1139, 1233
Sse9I AATT 8 cut(s) 260, 443, 756, 872, 918, 1041, 1081, 1191
SseBI AGGCCT 1 cut(s) 688
SspMI CTAG 1 cut(s) 993
StuI AGGCCT 1 cut(s) 688
StyD4I CCNGG 5 cut(s) 683, 688, 1195, 1325, 1335
StyI CCWWGG 1 cut(s) 877
TaaI ACNGT 5 cut(s) 547, 865, 1027, 1242, 1419
TaiI ACGT 4 cut(s) 534, 832, 1287, 1367
TaqI TCGA 1 cut(s) 978
TasI AATT 8 cut(s) 260, 443, 756, 872, 918, 1041, 1081, 1191
TfiI GAWTC 3 cut(s) 848, 908, 1382
Tru1I TTAA 2 cut(s) 894, 966
Tru9I TTAA 2 cut(s) 894, 966
TscAI CASTG 4 cut(s) 621, 634, 1009, 1323
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 5 cut(s) 503, 704, 752, 788, 1301
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 5 cut(s) 36, 73, 249, 1053, 1235
TspGWI ACGGA 1 cut(s) 1397
TspRI CASTG 4 cut(s) 621, 634, 1009, 1323
VpaK11BI GGWCC 1 cut(s) 365
XapI RAATTY 4 cut(s) 260, 443, 872, 1081
XceI RCATGY 1 cut(s) 626
XcmI CCANNNNNNNNNTGG 1 cut(s) 1333
XmnI GAANNNNTTC 1 cut(s) 1191
XspI CTAG 1 cut(s) 993
Zsp2I ATGCAT 3 cut(s) 462, 1257, 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.