Rh2BG073200

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010736
Physical Location & Seq
Forward (+)
5548483 .. 5551244
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG073200.1

Sequence Viewer

Length: 1242 bp
ATGGATTTCCCCAAATGGGTTTCTTTGATTTTCATACTCTGTATGATTACGCCACTGCCCATTTCCTCAAACCAATCTTCGAGCCCAGAAGTGGAGCTGGCTCAAATCCCAGTCTTGTTTCTTGAACATGCTAAAAGAAACGAGCTTTTCGATCGTATGGTGAGTATCAGAAGGAAAATCCATGAAAACCCAGAGCTTTGTTATGAGGAATTTGAAACCAGTAAGCTTATCAGAGCTGAACTTGACCGGCTGGGCATCCCTTACAGATACCCTTTGGCTGAAACCGGCGTTGTTGGCCTTGTTGGGACCGGTGGTCCACCCTTTGTTGCAATCAGAGCAGACATGGATGCTTTGGCTATGCAGGAGAGTGTGGAGTGGGAGCACAAGAGTAAAATTCCAGGGAAGATGCATGGATGTGGACATGATGCTCATGTTGCCATGCTTCTTGGAGCTGCAACTATACTTCAAGAGTATCGCCACAAATTACAGGGGACTGTTGTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGCGCAGGAGCAAAGAAAATGATAGATGAAGGTGCAGTGGAGCATGTCAGTGCCATCTTTGGGTTACATGTCGCTGCTGACCACCCCATTGGTTCAGTGGCCGCCAGGCCTGGCCCTATTTTGGCTGCGAGTGGATTTTTTGAAGCCGTAATAAGTGGGAAGGGGGGTCATGCTGCAATTCCACAGCACACAATAGACCCAATCTTGGCTGCTTCCAATGTTATTGTTAGTCTGCAACATCTTGTATCACGTGAAGCCGACCCCTTGGATTCACAGGTTATTACTAGACAAGCCAGTGTTCAGAGGTGCAATGCAACTGTCTTATTCAATGAAATTGCACGGCCCTTTTACCCTGCAACTGTAAATGACAAAGATTTGCATGAGTACTTCCGGAAAGTTGCAGTTGATTTGGTGGGCCCTCAGAATGTATTTGAGAAGCAACCACTGATGGGGGCGGAAGACTTCTCATTCTATCAAGAAGTAATTCCAGGGTACTTCTTCTTTCTTGGTATGAAGAATGACACTCAAGGACAGTTCAAACCAATGCATTCACCATATTTCAGAGTAAACGAAGACGTGCTTCCTTATGGAGCTGCATTCCATGCATCACTGGCCGCCAGGTATCTCCTGGAAAAGCAACCGAAATCTACCTCAACAGCAGACCAAAAAACGAAATCCACTTCATTGAAAGGGAACTTTCATGATGAACTATGA

Protein Analysis

413

Amino Acids

45.53

Weight (kDa)

5.94

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 110 - 382 1e-44 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010736)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56650 AT5G56650 AT5G56660
fragaria_vesca FvH4_1g06730 FvH4_1g06730
malus_domestica MD02G1072200.v1.1 MD15G1202000.v1.1
prunus_persica Prupe.7G215300_v2.0.a1
pyrus_communis pycom02g05620 pycom15g17930
rosa_chinensis RchiOBHm_Chr2g0092471
rosa_laevigata RLG00000016315
rosa_multiflora Rmu_sc0004296.1_g000017 Rmu_sc0023787.1_g000002
rosa_roxburghii Rroxscaffold_2G00149070
rosa_rugosa Rorug02G0026900
rosa_samantha Rh2AG072700 Rh2BG073200 Rh2CG074500
rosa_wichuraiana Rw2G006020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 918
AciI CCGC 3 cut(s) 630, 983, 1143
AcoI YGGCCR 2 cut(s) 627, 1140
AcsI RAATTY 2 cut(s) 209, 393
AcvI CACGTG 1 cut(s) 779
AfaI GTAC 2 cut(s) 914, 1022
AfiI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 977
AflIII ACRYGT 1 cut(s) 595
AgeI ACCGGT 1 cut(s) 308
AgsI TTSAA 7 cut(s) 125, 215, 467, 671, 856, 1066, 1216
AhdI GACNNNNNGTC 1 cut(s) 312
AjiI CACGTC 1 cut(s) 1105
AjnI CCWGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
AluBI AGCT 8 cut(s) 97, 145, 196, 226, 236, 452, 518, 1121
AluI AGCT 8 cut(s) 97, 145, 196, 226, 236, 452, 518, 1121
Alw21I GWGCWC 1 cut(s) 384
Aor13HI TCCGGA 1 cut(s) 918
AoxI GGCC 7 cut(s) 295, 627, 635, 640, 869, 943, 1140
ApaI GGGCCC 1 cut(s) 947
ApeKI GCWGC 6 cut(s) 452, 602, 653, 701, 737, 1121
ApoI RAATTY 2 cut(s) 209, 393
AsiGI ACCGGT 1 cut(s) 308
Asp700I GAANNNNTTC 1 cut(s) 1011
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 6 cut(s) 306, 314, 641, 870, 943, 944
AsuHPI GGTGA 2 cut(s) 172, 1071
AvaII GGWCC 2 cut(s) 306, 314
BaeGI GKGCMC 1 cut(s) 947
BanII GRGCYC 2 cut(s) 86, 947
BbrPI CACGTG 1 cut(s) 779
BbsI GAAGAC 2 cut(s) 993, 1107
Bbv12I GWGCWC 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 6 cut(s) 439, 589, 640, 688, 724, 1108
BccI CCATC 2 cut(s) 590, 970
BceAI ACGGC 2 cut(s) 659, 884
BciT130I CCWGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
BfaI CTAG 1 cut(s) 813
BisI GCNGC 8 cut(s) 453, 603, 630, 654, 702, 738, 1122, 1143
BlsI GCNGC 8 cut(s) 454, 604, 631, 655, 703, 739, 1123, 1144
BmcAI AGTACT 1 cut(s) 914
Bme1390I CCNGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
Bme18I GGWCC 2 cut(s) 306, 314
BmeRI GACNNNNNGTC 1 cut(s) 312
BmgBI CACGTC 1 cut(s) 1105
BmgT120I GGNCC 6 cut(s) 306, 314, 641, 870, 943, 944
BmiI GGNNCC 2 cut(s) 307, 945
BmrFI CCNGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
BmrI ACTGGG 1 cut(s) 104
BmsI GCATC 5 cut(s) 264, 337, 396, 415, 1142
BmuI ACTGGG 1 cut(s) 104
BpiI GAAGAC 2 cut(s) 993, 1107
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 1038
BsaAI YACGTR 1 cut(s) 779
BsaJI CCNNGG 3 cut(s) 398, 792, 1016
BsaWI WCCGGW 2 cut(s) 308, 918
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 977
Bse118I RCCGGY 3 cut(s) 246, 284, 308
Bse1I ACTGG 4 cut(s) 110, 219, 822, 1143
Bse3DI GCAATG 1 cut(s) 844
BseAI TCCGGA 1 cut(s) 918
BseBI CCWGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
BseDI CCNNGG 3 cut(s) 398, 792, 1016
BseGI GGATG 3 cut(s) 255, 352, 419
BseLI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 977
BseMI GCAATG 1 cut(s) 844
BseMII CTCAG 2 cut(s) 510, 962
BseNI ACTGG 4 cut(s) 110, 219, 822, 1143
BseSI GKGCMC 1 cut(s) 947
BseXI GCAGC 6 cut(s) 439, 589, 640, 688, 724, 1108
BseYI CCCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 582
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 7 cut(s) 297, 629, 637, 642, 871, 945, 1142
BshTI ACCGGT 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 4 cut(s) 247, 285, 309, 919
BslFI GGGAC 2 cut(s) 319, 505
BslI CCNNNNNNNGG 6 cut(s) 16, 91, 588, 649, 733, 977
BsmFI GGGAC 2 cut(s) 319, 505
BsmI GAATGC 2 cut(s) 1075, 1124
BsnI GGCC 7 cut(s) 297, 629, 637, 642, 871, 945, 1142
Bsp120I GGGCCC 1 cut(s) 943
Bsp1286I GDGCHC 3 cut(s) 86, 384, 947
Bsp13I TCCGGA 1 cut(s) 918
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 3 cut(s) 630, 983, 1143
BspANI GGCC 7 cut(s) 297, 629, 637, 642, 871, 945, 1142
BspCNI CTCAG 2 cut(s) 511, 961
BspEI TCCGGA 1 cut(s) 918
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 2 cut(s) 307, 945
BsrDI GCAATG 1 cut(s) 844
BsrFI RCCGGY 3 cut(s) 246, 284, 308
BsrI ACTGG 4 cut(s) 110, 219, 822, 1143
BssAI RCCGGY 3 cut(s) 246, 284, 308
BssECI CCNNGG 3 cut(s) 398, 792, 1016
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 792
Bst2UI CCWGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
Bst4CI ACNGT 4 cut(s) 496, 847, 889, 1062
BstAPI GCANNNNNTGC 1 cut(s) 1130
BstBAI YACGTR 1 cut(s) 779
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 519, 948
BstF5I GGATG 3 cut(s) 255, 352, 419
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 1 cut(s) 154
BstMBI GATC 1 cut(s) 151
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1130, 1139
BstNI CCWGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
BstNSI RCATGY 3 cut(s) 131, 575, 599
BstSCI CCNGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
BstSLI GKGCMC 1 cut(s) 947
BstV1I GCAGC 6 cut(s) 439, 589, 640, 688, 724, 1108
BstV2I GAAGAC 2 cut(s) 993, 1107
BstXI CCANNNNNNTGG 1 cut(s) 617
BsuRI GGCC 7 cut(s) 297, 629, 637, 642, 871, 945, 1142
BtrI CACGTC 1 cut(s) 1105
BtsCI GGATG 3 cut(s) 255, 352, 419
BtsI GCAGTG 2 cut(s) 53, 570
BtsIMutI CAGTG 7 cut(s) 53, 570, 583, 630, 829, 971, 1136
Cac8I GCNNGC 1 cut(s) 99
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 1 cut(s) 533
Cfr10I RCCGGY 3 cut(s) 246, 284, 308
Cfr13I GGNCC 6 cut(s) 306, 314, 641, 870, 943, 944
Csp6I GTAC 2 cut(s) 913, 1021
CspAI ACCGGT 1 cut(s) 308
CviQI GTAC 2 cut(s) 913, 1021
DdeI CTNAG 2 cut(s) 519, 948
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
DriI GACNNNNNGTC 1 cut(s) 312
EaeI YGGCCR 2 cut(s) 627, 1140
Eam1105I GACNNNNNGTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 998
Eco130I CCWWGG 1 cut(s) 792
Eco147I AGGCCT 1 cut(s) 637
Eco24I GRGCYC 2 cut(s) 86, 947
Eco47I GGWCC 2 cut(s) 306, 314
Eco72I CACGTG 1 cut(s) 779
EcoO109I RGGNCCY 1 cut(s) 944
EcoRII CCWGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
EcoT14I CCWWGG 1 cut(s) 792
EcoT22I ATGCAT 3 cut(s) 411, 1077, 1135
EcoT38I GRGCYC 2 cut(s) 86, 947
ErhI CCWWGG 1 cut(s) 792
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FaqI GGGAC 2 cut(s) 319, 505
Fnu4HI GCNGC 8 cut(s) 453, 603, 630, 654, 702, 738, 1122, 1143
FokI GGATG 3 cut(s) 242, 359, 426
FriOI GRGCYC 2 cut(s) 86, 947
Fsp4HI GCNGC 8 cut(s) 453, 603, 630, 654, 702, 738, 1122, 1143
FspBI CTAG 1 cut(s) 813
GlaI GCGC 1 cut(s) 532
GluI GCNGC 8 cut(s) 453, 603, 630, 654, 702, 738, 1122, 1143
GsaI CCCAGC 1 cut(s) 254
HaeIII GGCC 7 cut(s) 297, 629, 637, 642, 871, 945, 1142
HapII CCGG 4 cut(s) 247, 285, 309, 919
HhaI GCGC 1 cut(s) 533
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 1 cut(s) 797
HpaII CCGG 4 cut(s) 247, 285, 309, 919
HphI GGTGA 2 cut(s) 172, 1071
Hpy166II GTNNAC 3 cut(s) 317, 419, 1096
Hpy188I TCNGA 6 cut(s) 170, 233, 335, 831, 951, 1091
Hpy188III TCNNGA 5 cut(s) 122, 467, 919, 1004, 1229
Hpy8I GTNNAC 3 cut(s) 317, 419, 1096
HpyAV CCTTC 4 cut(s) 165, 518, 551, 682
HpyCH4III ACNGT 4 cut(s) 496, 847, 889, 1062
HpyCH4IV ACGT 2 cut(s) 778, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1130, 1139
HpyF3I CTNAG 2 cut(s) 519, 948
HpySE526I ACGT 2 cut(s) 778, 1104
HspAI GCGC 1 cut(s) 531
Kpn2I TCCGGA 1 cut(s) 918
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 6 cut(s) 94, 379, 449, 536, 568, 1118
Lsp1109I GCAGC 6 cut(s) 439, 589, 640, 688, 724, 1108
LweI GCATC 5 cut(s) 264, 337, 396, 415, 1142
MaeI CTAG 1 cut(s) 813
MaeII ACGT 2 cut(s) 778, 1104
MaeIII GTNAC 1 cut(s) 591
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 6 cut(s) 69, 415, 998, 1018, 1054, 1112
MhlI GDGCHC 3 cut(s) 86, 384, 947
MluCI AATT 6 cut(s) 209, 393, 482, 705, 861, 1011
MmeI TCCRAC 1 cut(s) 535
MnlI CCTC 7 cut(s) 76, 199, 514, 521, 825, 957, 1189
Mph1103I ATGCAT 3 cut(s) 411, 1077, 1135
MroI TCCGGA 1 cut(s) 918
MroXI GAANNNNTTC 1 cut(s) 1011
MslI CAYNNNNRTG 2 cut(s) 414, 576
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 4 cut(s) 247, 285, 309, 919
MspR9I CCNGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
Mva1269I GAATGC 2 cut(s) 1075, 1124
MvaI CCWGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
MwoI GCNNNNNNNGC 5 cut(s) 294, 335, 434, 1130, 1139
NdeII GATC 1 cut(s) 151
NlaIV GGNNCC 2 cut(s) 307, 945
NsiI ATGCAT 3 cut(s) 411, 1077, 1135
NspI RCATGY 3 cut(s) 131, 575, 599
PagI TCATGA 1 cut(s) 1228
PceI AGGCCT 1 cut(s) 637
PciI ACATGT 1 cut(s) 595
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 1075, 1124
PdmI GAANNNNTTC 1 cut(s) 1011
PfeI GAWTC 1 cut(s) 797
PfoI TCCNGGA 1 cut(s) 1155
PinAI ACCGGT 1 cut(s) 308
PkrI GCNGC 8 cut(s) 454, 604, 631, 655, 703, 739, 1123, 1144
Ple19I CGATCG 1 cut(s) 154
PmaCI CACGTG 1 cut(s) 779
PmlI CACGTG 1 cut(s) 779
Ppu21I YACGTR 1 cut(s) 779
PscI ACATGT 1 cut(s) 595
Psp6I CCWGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
PspCI CACGTG 1 cut(s) 779
PspFI CCCAGC 1 cut(s) 250
PspGI CCWGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
PspN4I GGNNCC 2 cut(s) 307, 945
PspOMI GGGCCC 1 cut(s) 943
PspPI GGNCC 6 cut(s) 306, 314, 641, 870, 943, 944
PvuI CGATCG 1 cut(s) 154
PvuII CAGCTG 1 cut(s) 518
RsaI GTAC 2 cut(s) 914, 1022
RsaNI GTAC 2 cut(s) 913, 1021
RseI CAYNNNNRTG 2 cut(s) 414, 576
SatI GCNGC 8 cut(s) 453, 603, 630, 654, 702, 738, 1122, 1143
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 6 cut(s) 306, 314, 641, 870, 943, 944
ScaI AGTACT 1 cut(s) 914
ScrFI CCNGG 6 cut(s) 399, 634, 639, 1017, 1147, 1157
SduI GDGCHC 3 cut(s) 86, 384, 947
SfaNI GCATC 5 cut(s) 264, 337, 396, 415, 1142
SinI GGWCC 2 cut(s) 306, 314
SmiMI CAYNNNNRTG 2 cut(s) 414, 576
SmlI CTYRAG 1 cut(s) 1053
SmoI CTYRAG 1 cut(s) 1053
Sse9I AATT 6 cut(s) 209, 393, 482, 705, 861, 1011
SseBI AGGCCT 1 cut(s) 637
SsiI CCGC 3 cut(s) 630, 983, 1143
SspMI CTAG 1 cut(s) 813
StuI AGGCCT 1 cut(s) 637
StyD4I CCNGG 6 cut(s) 397, 632, 637, 1015, 1145, 1155
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 4 cut(s) 496, 847, 889, 1062
TaiI ACGT 2 cut(s) 781, 1107
TaqI TCGA 2 cut(s) 80, 150
TasI AATT 6 cut(s) 209, 393, 482, 705, 861, 1011
TatI WGTACW 1 cut(s) 912
TauI GCSGC 2 cut(s) 632, 1145
TfiI GAWTC 1 cut(s) 797
TscAI CASTG 7 cut(s) 60, 570, 583, 630, 829, 978, 1143
TseI GCWGC 6 cut(s) 452, 602, 653, 701, 737, 1121
TspDTI ATGAA 7 cut(s) 22, 198, 570, 873, 1055, 1200, 1217
TspRI CASTG 7 cut(s) 60, 570, 583, 630, 829, 978, 1143
VpaK11BI GGWCC 2 cut(s) 306, 314
XapI RAATTY 2 cut(s) 209, 393
XceI RCATGY 3 cut(s) 131, 575, 599
XcmI CCANNNNNNNNNTGG 2 cut(s) 622, 1153
XmnI GAANNNNTTC 1 cut(s) 1011
XspI CTAG 1 cut(s) 813
ZrmI AGTACT 1 cut(s) 914
Zsp2I ATGCAT 3 cut(s) 411, 1077, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.