Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010736

Physical Location & Seq

Genomic Coordinates Forward (+)

16070493 .. 16073945

Gene Structure 3453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1202000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGATTTGCATCCTACACCGTTCTACAAAATTTCAACCCCACTCTCCATATCCATCACCACTTCCACAAAGCTCACCGTGCAAATCCATGGCGTCCTGCAAATGGGTCCCCCCGATTTTTACTGTTTTACTGATTCTCCTGCTTCTCATTTCTTCAGCCAGTTCTTTGACCCCGGAAGAGTTGGCCCAAATCCCGGTGAAGTTCCTTGACTTCGCCAAAAGGCAGGAGCTTTTCGATTACATGGTGGGAGTCAGAAGGAAAATACATGAAAACCCAGAACTTGGTTTCGAGGAATTCGAGACCAGTAAGCTTATCAGAGACGAACTTGATCAGATGGACGTCCGTTACAAATACCCATTCGCCGGAACCGGCGTTGTTGGCTTTGTTGGGAGTGGTGGACCGCCGTTCGTTGCGATTCGAGCGGATATGGATGCTCTGGCTATGCAGAGTGTGGAGTGGGAGCACAAGAGCAAAGTTGCAGGGAAGATGCATGCTTGTGGACATGATGCTCATGTTGCCCATGCTTCTTGGTGCTGCAAAGATACTTCAAGAACATCGACACGAATTACAGGTGTTATTGGACGACTTGTTAAAATAATGAATGTATATAATGGTTCTAAATGTCATCAATTGCATTGCGAGTCTGGTTGCTTGATTGGTTTGAATCTGTTGTGTTCTGTTTACTCGTTAGTTTTATGGGGAACGGTTGTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGTGGAGGAGCAAAGAAGATGGTAGATGAAGGGGCTCTGGAGAATGTTGGTGCCATCTTTGGGTTGCATGTCGCCGCTAATCACCCCATTGGTTCAGTGGCCTCCAGGTCTGGCCCTTTTTTTGCTGCCAGTGGTTTTTTTGAGGCGGTAATAAGTGGGAAGGGGGGTCACGCTGCCATTCCACAGCACACAATAGACCCGATCTTGGCCGCTTCATATGTTATTGTTAGTCTGCAGCATCTTGTTTCACGTGAAGCCAACCCCTTGGATTCACAGGTTGTGACTGTTGGAAAATTCCGGGGAGGCGGTGCTTTCAATGTCATTCCAGATTCTGTTACAATTGGTGGGACGTTCAGGGCATTTTCGAAGGAAAGCTTTATGCATCTTAAACAAAGAATTGAAGAGGTCATCACTAGACAAGCCAGCGTACAGAGGTGCAATGCAACTGTCCTATTCAACGAAAAGGCTAAGCCCTTTTACCCCGTAGTCATTAACGACAGAGATCTGCATCAACACTTCCTAAATGTTGCGGGAGATTTGCTAGGCCCTCGGAACATAGTTGAGAGGCAACCAACGATGGGTGCAGAAGACTTCGCATTTTATCAAGAAGTAATTCCAGGATACTTCTTCGCGGTCGGTATGAAGAACGAGTCTCAAGGAGAGCTCGAATCAGGGCATTCACCGAACTTCAGAGTAAACGAAGATGTGCTTCCGTATGGAGCGGCATTACATGTATCCTTGGCCACAAGGTACCTACTTGAAAATCAACCGGAATCTACTTCGTCTAAAGGGAACTCTCAAGATGAATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010179 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

517

Amino Acids

56.39

Weight (kDa)

6.47

Isoelectric Point (pI)

43.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	138 - 174	1.5e-06	Peptidase family M20/M25/M40
Peptidase_M20	PF01546	234 - 495	2.7e-22	Peptidase family M20/M25/M40
M20_dimer	PF07687	294 - 386	7.3e-11	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010736)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56650 AT5G56650 AT5G56660
fragaria_vesca	FvH4_1g06730 FvH4_1g06730
malus_domestica	MD02G1072200.v1.1 MD15G1202000.v1.1
prunus_persica	Prupe.7G215300_v2.0.a1
pyrus_communis	pycom02g05620 pycom15g17930
rosa_chinensis	RchiOBHm_Chr2g0092471
rosa_laevigata	RLG00000016315
rosa_multiflora	Rmu_sc0004296.1_g000017 Rmu_sc0023787.1_g000002
rosa_roxburghii	Rroxscaffold_2G00149070
rosa_rugosa	Rorug02G0026900
rosa_samantha	Rh2AG072700 Rh2BG073200 Rh2CG074500
rosa_wichuraiana	Rw2G006020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	342
Acc65I	GGTACC	1 cut(s)	1488
AccB1I	GGYRCC	2 cut(s)	788, 1488
AccB7I	CCANNNNNTGG	1 cut(s)	281
AccBSI	CCGCTC	2 cut(s)	422, 1460
AccII	CGCG	1 cut(s)	1370
AciI	CCGC	9 cut(s)	401, 422, 813, 884, 948, 1044, 1268, 1370, 1460
AcoI	YGGCCR	2 cut(s)	945, 1479
AcsI	RAATTY	3 cut(s)	29, 293, 1031
AcuI	CTGAAG	2 cut(s)	138, 1411
AcvI	CACGTG	1 cut(s)	989
AcyI	GRCGYC	2 cut(s)	92, 339
AfaI	GTAC	2 cut(s)	1167, 1490
AfiI	CCNNNNNNNGG	7 cut(s)	102, 193, 281, 362, 798, 943, 1316
AflIII	ACRYGT	1 cut(s)	1468
AgsI	TTSAA	7 cut(s)	35, 549, 664, 1054, 1139, 1195, 1499
AjnI	CCWGG	2 cut(s)	842, 1354
AloI	GAACNNNNNNTCC	2 cut(s)	693, 725
AluBI	AGCT	6 cut(s)	72, 229, 310, 728, 1113, 1402
AluI	AGCT	6 cut(s)	72, 229, 310, 728, 1113, 1402
Alw21I	GWGCWC	2 cut(s)	465, 1404
Alw26I	GTCTC	3 cut(s)	293, 312, 1395
AlwNI	CAGNNNCTG	1 cut(s)	1070
AoxI	GGCC	6 cut(s)	183, 837, 850, 945, 1282, 1479
ApeKI	GCWGC	4 cut(s)	534, 863, 911, 973
ApoI	RAATTY	3 cut(s)	29, 293, 1031
Asp700I	GAANNNNTTC	1 cut(s)	1350
Asp718I	GGTACC	1 cut(s)	1488
AspS9I	GGNCC	5 cut(s)	106, 184, 398, 851, 1283
AsuC2I	CCSGG	3 cut(s)	173, 194, 1037
AsuHPI	GGTGA	5 cut(s)	48, 66, 208, 812, 1410
AsuII	TTCGAA	1 cut(s)	1103
AvaII	GGWCC	2 cut(s)	106, 398
BalI	TGGCCA	1 cut(s)	1481
BanI	GGYRCC	2 cut(s)	788, 1488
BanII	GRGCYC	2 cut(s)	775, 1404
BbrPI	CACGTG	1 cut(s)	989
BbsI	GAAGAC	1 cut(s)	1332
Bbv12I	GWGCWC	2 cut(s)	465, 1404
BbvCI	CCTCAGC	1 cut(s)	729
BbvI	GCAGC	4 cut(s)	521, 850, 898, 985
BccI	CCATC	5 cut(s)	61, 328, 751, 800, 1309
BceAI	ACGGC	1 cut(s)	388
BciT130I	CCWGG	2 cut(s)	844, 1356
BciVI	GTATCC	2 cut(s)	1352, 1483
BclI	TGATCA	1 cut(s)	328
BcnI	CCSGG	3 cut(s)	173, 194, 1037
BcoDI	GTCTC	3 cut(s)	293, 312, 1395
BfaI	CTAG	2 cut(s)	1152, 1280
BfmI	CTRYAG	1 cut(s)	971
BfuI	GTATCC	2 cut(s)	1352, 1483
BglII	AGATCT	1 cut(s)	1240
BisI	GCNGC	7 cut(s)	535, 813, 864, 912, 948, 974, 1461
BlpI	GCTNAGC	1 cut(s)	1206
BlsI	GCNGC	7 cut(s)	536, 814, 865, 913, 949, 975, 1462
Bme1390I	CCNGG	5 cut(s)	173, 194, 844, 1037, 1356
Bme18I	GGWCC	2 cut(s)	106, 398
BmgT120I	GGNCC	5 cut(s)	106, 184, 398, 851, 1283
BmiI	GGNNCC	5 cut(s)	107, 108, 367, 790, 1490
BmrFI	CCNGG	5 cut(s)	173, 194, 844, 1037, 1356
BmsI	GCATC	7 cut(s)	18, 421, 477, 496, 985, 1129, 1255
BpiI	GAAGAC	1 cut(s)	1332
BpmI	CTGGAG	2 cut(s)	797, 826
Bpu10I	CCTNAGC	1 cut(s)	729
Bpu1102I	GCTNAGC	1 cut(s)	1206
Bpu14I	TTCGAA	1 cut(s)	1103
BpuEI	CTTGAG	2 cut(s)	1377, 1521
BpuMI	CCSGG	3 cut(s)	173, 194, 1037
BsaAI	YACGTR	1 cut(s)	989
BsaBI	GATNNNNATC	2 cut(s)	8, 1245
BsaHI	GRCGYC	2 cut(s)	92, 339
BsaI	GGTCTC	1 cut(s)	293
BsaJI	CCNNGG	6 cut(s)	87, 171, 1002, 1036, 1286, 1476
BsaWI	WCCGGW	1 cut(s)	1507
BsaXI	ACNNNNNCTCC	2 cut(s)	120, 150
Bsc4I	CCNNNNNNNGG	7 cut(s)	102, 193, 281, 362, 798, 943, 1316
Bse118I	RCCGGY	1 cut(s)	368
Bse1I	ACTGG	3 cut(s)	159, 303, 867
Bse3DI	GCAATG	2 cut(s)	634, 1183
Bse8I	GATNNNNATC	2 cut(s)	8, 1245
BseBI	CCWGG	2 cut(s)	844, 1356
BseDI	CCNNGG	6 cut(s)	87, 171, 1002, 1036, 1286, 1476
BseGI	GGATG	2 cut(s)	9, 436
BseJI	GATNNNNATC	2 cut(s)	8, 1245
BseLI	CCNNNNNNNGG	7 cut(s)	102, 193, 281, 362, 798, 943, 1316
BseMI	GCAATG	2 cut(s)	634, 1183
BseMII	CTCAG	1 cut(s)	720
BseNI	ACTGG	3 cut(s)	159, 303, 867
BseRI	GAGGAG	1 cut(s)	759
BseXI	GCAGC	4 cut(s)	521, 850, 898, 985
BsgI	GTGCAG	1 cut(s)	1341
Bsh1236I	CGCG	1 cut(s)	1370
Bsh1285I	CGRYCG	1 cut(s)	1374
BshFI	GGCC	6 cut(s)	185, 839, 852, 947, 1284, 1481
BshNI	GGYRCC	2 cut(s)	788, 1488
BsiEI	CGRYCG	1 cut(s)	1374
BsiHKAI	GWGCWC	2 cut(s)	465, 1404
BsiSI	CCGG	6 cut(s)	173, 194, 363, 369, 1036, 1508
BslFI	GGGAC	2 cut(s)	92, 1099
BslI	CCNNNNNNNGG	7 cut(s)	102, 193, 281, 362, 798, 943, 1316
BsmAI	GTCTC	3 cut(s)	293, 312, 1395
BsmBI	CGTCTC	1 cut(s)	312
BsmFI	GGGAC	2 cut(s)	92, 1099
BsmI	GAATGC	1 cut(s)	1414
BsnI	GGCC	6 cut(s)	185, 839, 852, 947, 1284, 1481
Bso31I	GGTCTC	1 cut(s)	293
Bsp119I	TTCGAA	1 cut(s)	1103
Bsp1286I	GDGCHC	3 cut(s)	465, 775, 1404
Bsp143I	GATC	3 cut(s)	328, 939, 1240
Bsp1720I	GCTNAGC	1 cut(s)	1206
Bsp19I	CCATGG	1 cut(s)	87
BspACI	CCGC	9 cut(s)	401, 422, 813, 884, 948, 1044, 1268, 1370, 1460
BspANI	GGCC	6 cut(s)	185, 839, 852, 947, 1284, 1481
BspCNI	CTCAG	1 cut(s)	721
BspFNI	CGCG	1 cut(s)	1370
BspLI	GGNNCC	5 cut(s)	107, 108, 367, 790, 1490
BspMAI	CTGCAG	1 cut(s)	975
BspT104I	TTCGAA	1 cut(s)	1103
BspT107I	GGYRCC	2 cut(s)	788, 1488
BspTNI	GGTCTC	1 cut(s)	293
BsrBI	CCGCTC	2 cut(s)	422, 1460
BsrDI	GCAATG	2 cut(s)	634, 1183
BsrFI	RCCGGY	1 cut(s)	368
BsrI	ACTGG	3 cut(s)	159, 303, 867
BssAI	RCCGGY	1 cut(s)	368
BssECI	CCNNGG	6 cut(s)	87, 171, 1002, 1036, 1286, 1476
BssMI	GATC	3 cut(s)	328, 939, 1240
BssNI	GRCGYC	2 cut(s)	92, 339
BssT1I	CCWWGG	3 cut(s)	87, 1002, 1476
Bst2UI	CCWGG	2 cut(s)	844, 1356
Bst4CI	ACNGT	6 cut(s)	20, 78, 124, 706, 1024, 1186
Bst6I	CTCTTC	2 cut(s)	171, 1134
BstACI	GRCGYC	2 cut(s)	92, 339
BstBAI	YACGTR	1 cut(s)	989
BstBI	TTCGAA	1 cut(s)	1103
BstC8I	GCNNGC	2 cut(s)	492, 1162
BstDEI	CTNAG	2 cut(s)	729, 1206
BstDSI	CCRYGG	1 cut(s)	87
BstF5I	GGATG	2 cut(s)	9, 436
BstFNI	CGCG	1 cut(s)	1370
BstKTI	GATC	3 cut(s)	331, 942, 1243
BstMAI	GTCTC	3 cut(s)	293, 312, 1395
BstMBI	GATC	3 cut(s)	328, 939, 1240
BstMCI	CGRYCG	1 cut(s)	1374
BstMWI	GCNNNNNNNGC	4 cut(s)	78, 378, 419, 515
BstNI	CCWGG	2 cut(s)	844, 1356
BstNSI	RCATGY	3 cut(s)	494, 809, 1472
BstSCI	CCNGG	5 cut(s)	171, 192, 842, 1035, 1354
BstSFI	CTRYAG	1 cut(s)	971
BstUI	CGCG	1 cut(s)	1370
BstV1I	GCAGC	4 cut(s)	521, 850, 898, 985
BstV2I	GAAGAC	1 cut(s)	1332
BstX2I	RGATCY	1 cut(s)	1240
BstXI	CCANNNNNNTGG	1 cut(s)	1003
BstYI	RGATCY	1 cut(s)	1240
BsuI	GTATCC	2 cut(s)	1352, 1483
BsuRI	GGCC	6 cut(s)	185, 839, 852, 947, 1284, 1481
BtgI	CCRYGG	1 cut(s)	87
BtsCI	GGATG	2 cut(s)	9, 436
BtsIMutI	CAGTG	2 cut(s)	840, 874
Cac8I	GCNNGC	2 cut(s)	492, 1162
CaiI	CAGNNNCTG	1 cut(s)	1070
Cfr10I	RCCGGY	1 cut(s)	368
Cfr13I	GGNCC	5 cut(s)	106, 184, 398, 851, 1283
CseI	GACGC	1 cut(s)	81
Csp6I	GTAC	2 cut(s)	1166, 1489
CviAII	CATG	9 cut(s)	88, 241, 266, 491, 503, 512, 521, 806, 1469
CviQI	GTAC	2 cut(s)	1166, 1489
DdeI	CTNAG	2 cut(s)	729, 1206
DpnI	GATC	3 cut(s)	330, 941, 1242
DpnII	GATC	3 cut(s)	328, 939, 1240
EaeI	YGGCCR	2 cut(s)	945, 1479
Eam1104I	CTCTTC	2 cut(s)	171, 1134
EarI	CTCTTC	2 cut(s)	171, 1134
Ecl136II	GAGCTC	1 cut(s)	1402
Eco130I	CCWWGG	3 cut(s)	87, 1002, 1476
Eco24I	GRGCYC	2 cut(s)	775, 1404
Eco31I	GGTCTC	1 cut(s)	293
Eco47I	GGWCC	2 cut(s)	106, 398
Eco53kI	GAGCTC	1 cut(s)	1402
Eco57I	CTGAAG	2 cut(s)	138, 1411
Eco72I	CACGTG	1 cut(s)	989
EcoICRI	GAGCTC	1 cut(s)	1402
EcoO109I	RGGNCCY	2 cut(s)	106, 1283
EcoRI	GAATTC	1 cut(s)	293
EcoRII	CCWGG	2 cut(s)	842, 1354
EcoT14I	CCWWGG	3 cut(s)	87, 1002, 1476
EcoT22I	ATGCAT	2 cut(s)	492, 1122
EcoT38I	GRGCYC	2 cut(s)	775, 1404
ErhI	CCWWGG	3 cut(s)	87, 1002, 1476
Esp3I	CGTCTC	1 cut(s)	312
FaeI	CATG	9 cut(s)	91, 244, 269, 494, 506, 515, 524, 809, 1472
FalI	AAGNNNNNCTT	4 cut(s)	1097, 1129, 1431, 1463
FaqI	GGGAC	2 cut(s)	92, 1099
FatI	CATG	9 cut(s)	87, 240, 265, 490, 502, 511, 520, 805, 1468
FauI	CCCGC	1 cut(s)	1261
FauNDI	CATATG	1 cut(s)	955
FbaI	TGATCA	1 cut(s)	328
Fnu4HI	GCNGC	7 cut(s)	535, 813, 864, 912, 948, 974, 1461
FokI	GGATG	1 cut(s)	443
FriOI	GRGCYC	2 cut(s)	775, 1404
Fsp4HI	GCNGC	7 cut(s)	535, 813, 864, 912, 948, 974, 1461
FspBI	CTAG	2 cut(s)	1152, 1280
GluI	GCNGC	7 cut(s)	535, 813, 864, 912, 948, 974, 1461
GsuI	CTGGAG	2 cut(s)	797, 826
HaeIII	GGCC	6 cut(s)	185, 839, 852, 947, 1284, 1481
HapII	CCGG	6 cut(s)	173, 194, 363, 369, 1036, 1508
HgaI	GACGC	1 cut(s)	81
Hin1I	GRCGYC	2 cut(s)	92, 339
Hin1II	CATG	9 cut(s)	91, 244, 269, 494, 506, 515, 524, 809, 1472
HindIII	AAGCTT	2 cut(s)	308, 1111
HpaII	CCGG	6 cut(s)	173, 194, 363, 369, 1036, 1508
HphI	GGTGA	5 cut(s)	48, 66, 208, 812, 1410
Hpy166II	GTNNAC	4 cut(s)	398, 500, 682, 1435
Hpy188I	TCNGA	5 cut(s)	254, 317, 333, 1290, 1430
Hpy188III	TCNNGA	6 cut(s)	298, 549, 776, 1064, 1343, 1538
Hpy8I	GTNNAC	4 cut(s)	398, 500, 682, 1435
HpyAV	CCTTC	5 cut(s)	249, 728, 761, 892, 1099
HpyCH4III	ACNGT	6 cut(s)	20, 78, 124, 706, 1024, 1186
HpyCH4IV	ACGT	3 cut(s)	339, 988, 1088
HpyF10VI	GCNNNNNNNGC	4 cut(s)	78, 378, 419, 515
HpyF3I	CTNAG	2 cut(s)	729, 1206
HpySE526I	ACGT	3 cut(s)	339, 988, 1088
Hsp92I	GRCGYC	2 cut(s)	92, 339
Hsp92II	CATG	9 cut(s)	91, 244, 269, 494, 506, 515, 524, 809, 1472
KflI	GGGWCCC	1 cut(s)	106
KpnI	GGTACC	1 cut(s)	1492
Ksp22I	TGATCA	1 cut(s)	328
Kzo9I	GATC	3 cut(s)	328, 939, 1240
LmnI	GCTCC	4 cut(s)	226, 460, 746, 1457
Lsp1109I	GCAGC	4 cut(s)	521, 850, 898, 985
LweI	GCATC	7 cut(s)	18, 421, 477, 496, 985, 1129, 1255
MaeI	CTAG	2 cut(s)	1152, 1280
MaeII	ACGT	3 cut(s)	339, 988, 1088
MaeIII	GTNAC	4 cut(s)	344, 905, 1018, 1072
MalI	GATC	3 cut(s)	330, 941, 1242
MbiI	CCGCTC	2 cut(s)	422, 1460
MboI	GATC	3 cut(s)	328, 939, 1240
MboII	GAAGA	9 cut(s)	144, 188, 496, 766, 1151, 1337, 1357, 1393, 1451
MfeI	CAATTG	2 cut(s)	629, 1077
MflI	RGATCY	1 cut(s)	1240
MhlI	GDGCHC	3 cut(s)	465, 775, 1404
MlsI	TGGCCA	1 cut(s)	1481
MluCI	AATT	9 cut(s)	29, 293, 564, 629, 1031, 1077, 1134, 1350, 1544
MluNI	TGGCCA	1 cut(s)	1481
MlyI	GAGTC	3 cut(s)	258, 650, 1397
MmeI	TCCRAC	2 cut(s)	745, 1006
Mox20I	TGGCCA	1 cut(s)	1481
Mph1103I	ATGCAT	2 cut(s)	492, 1122
MroXI	GAANNNNTTC	1 cut(s)	1350
MscI	TGGCCA	1 cut(s)	1481
MseI	TTAA	3 cut(s)	591, 1125, 1230
MslI	CAYNNNNRTG	1 cut(s)	495
Msp20I	TGGCCA	1 cut(s)	1481
MspA1I	CMGCKG	1 cut(s)	728
MspI	CCGG	6 cut(s)	173, 194, 363, 369, 1036, 1508
MspR9I	CCNGG	5 cut(s)	173, 194, 844, 1037, 1356
MunI	CAATTG	2 cut(s)	629, 1077
Mva1269I	GAATGC	1 cut(s)	1414
MvaI	CCWGG	2 cut(s)	844, 1356
MvnI	CGCG	1 cut(s)	1370
MwoI	GCNNNNNNNGC	4 cut(s)	78, 378, 419, 515
NciI	CCSGG	3 cut(s)	173, 194, 1037
NcoI	CCATGG	1 cut(s)	87
NdeI	CATATG	1 cut(s)	955
NdeII	GATC	3 cut(s)	328, 939, 1240
NlaIII	CATG	9 cut(s)	91, 244, 269, 494, 506, 515, 524, 809, 1472
NlaIV	GGNNCC	5 cut(s)	107, 108, 367, 790, 1490
NmuCI	GTSAC	2 cut(s)	905, 1018
NsiI	ATGCAT	2 cut(s)	492, 1122
NspI	RCATGY	3 cut(s)	494, 809, 1472
NspV	TTCGAA	1 cut(s)	1103
PaeI	GCATGC	1 cut(s)	494
PciI	ACATGT	1 cut(s)	1468
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PctI	GAATGC	1 cut(s)	1414
PdmI	GAANNNNTTC	1 cut(s)	1350
PfeI	GAWTC	7 cut(s)	133, 415, 664, 1007, 1067, 1406, 1511
PflMI	CCANNNNNTGG	1 cut(s)	281
PfoI	TCCNGGA	1 cut(s)	1354
PkrI	GCNGC	7 cut(s)	536, 814, 865, 913, 949, 975, 1462
PleI	GAGTC	3 cut(s)	257, 649, 1396
PmaCI	CACGTG	1 cut(s)	989
PmlI	CACGTG	1 cut(s)	989
PpsI	GAGTC	3 cut(s)	257, 649, 1396
Ppu21I	YACGTR	1 cut(s)	989
PpuMI	RGGWCCY	1 cut(s)	106
PscI	ACATGT	1 cut(s)	1468
Psp124BI	GAGCTC	1 cut(s)	1404
Psp5II	RGGWCCY	1 cut(s)	106
Psp6I	CCWGG	2 cut(s)	842, 1354
PspCI	CACGTG	1 cut(s)	989
PspGI	CCWGG	2 cut(s)	842, 1354
PspN4I	GGNNCC	5 cut(s)	107, 108, 367, 790, 1490
PspPI	GGNCC	5 cut(s)	106, 184, 398, 851, 1283
PspPPI	RGGWCCY	1 cut(s)	106
PstI	CTGCAG	1 cut(s)	975
PstNI	CAGNNNCTG	1 cut(s)	1070
PsuI	RGATCY	1 cut(s)	1240
PvuII	CAGCTG	1 cut(s)	728
RsaI	GTAC	2 cut(s)	1167, 1490
RsaNI	GTAC	2 cut(s)	1166, 1489
RseI	CAYNNNNRTG	1 cut(s)	495
SacI	GAGCTC	1 cut(s)	1404
SaqAI	TTAA	3 cut(s)	591, 1125, 1230
SatI	GCNGC	7 cut(s)	535, 813, 864, 912, 948, 974, 1461
Sau3AI	GATC	3 cut(s)	328, 939, 1240
Sau96I	GGNCC	5 cut(s)	106, 184, 398, 851, 1283
SchI	GAGTC	3 cut(s)	258, 650, 1397
ScrFI	CCNGG	5 cut(s)	173, 194, 844, 1037, 1356
SduI	GDGCHC	3 cut(s)	465, 775, 1404
SfaNI	GCATC	7 cut(s)	18, 421, 477, 496, 985, 1129, 1255
SfcI	CTRYAG	1 cut(s)	971
SfuI	TTCGAA	1 cut(s)	1103
SinI	GGWCC	2 cut(s)	106, 398
SmiMI	CAYNNNNRTG	1 cut(s)	495
SmlI	CTYRAG	2 cut(s)	1392, 1536
SmoI	CTYRAG	2 cut(s)	1392, 1536
SphI	GCATGC	1 cut(s)	494
Sse9I	AATT	9 cut(s)	29, 293, 564, 629, 1031, 1077, 1134, 1350, 1544
SsiI	CCGC	9 cut(s)	401, 422, 813, 884, 948, 1044, 1268, 1370, 1460
SspMI	CTAG	2 cut(s)	1152, 1280
SstI	GAGCTC	1 cut(s)	1404
StyD4I	CCNGG	5 cut(s)	171, 192, 842, 1035, 1354
StyI	CCWWGG	3 cut(s)	87, 1002, 1476
TaaI	ACNGT	6 cut(s)	20, 78, 124, 706, 1024, 1186
TaiI	ACGT	3 cut(s)	342, 991, 1091
TaqI	TCGA	7 cut(s)	234, 288, 297, 418, 557, 1103, 1404
TasI	AATT	9 cut(s)	29, 293, 564, 629, 1031, 1077, 1134, 1350, 1544
TauI	GCSGC	3 cut(s)	815, 950, 1463
TfiI	GAWTC	7 cut(s)	133, 415, 664, 1007, 1067, 1406, 1511
Tru1I	TTAA	3 cut(s)	591, 1125, 1230
Tru9I	TTAA	3 cut(s)	591, 1125, 1230
TscAI	CASTG	2 cut(s)	840, 874
TseFI	GTSAC	2 cut(s)	905, 1018
TseI	GCWGC	4 cut(s)	534, 863, 911, 973
Tsp45I	GTSAC	2 cut(s)	905, 1018
TspDTI	ATGAA	5 cut(s)	282, 614, 780, 942, 1394
TspGWI	ACGGA	2 cut(s)	332, 1440
TspRI	CASTG	2 cut(s)	840, 874
Van91I	CCANNNNNTGG	1 cut(s)	281
VpaK11BI	GGWCC	2 cut(s)	106, 398
XapI	RAATTY	3 cut(s)	29, 293, 1031
XceI	RCATGY	3 cut(s)	494, 809, 1472
XcmI	CCANNNNNNNNNTGG	1 cut(s)	832
XmnI	GAANNNNTTC	1 cut(s)	1350
XspI	CTAG	2 cut(s)	1152, 1280
ZraI	GACGTC	1 cut(s)	340
Zsp2I	ATGCAT	2 cut(s)	492, 1122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.