Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0010736

Physical Location & Seq

Genomic Coordinates Forward (+)

5979660 .. 5982895

Gene Structure 3236 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ46758

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGTCACGACTCACTCACCCGCTGCCTTTCGTCTCGGAGTCCCCTGTCCCATTCTGATTCTGATTCTGATTCTGATTCTGATTCTGACATACATTTTCAACCTTCCAATCTTGAAATTCAATCATTCATTCTTAACATCAATGGATTTCCCCAAATGGGTTTCTTTGATTTTCATACTCTGTATGATTACGCCACTGCCCATTTCCTCAAAGCAATCTTCGAGCCCAGAAGTGGAGCTGGCTCAAATCCCAGTCTTGTTTCTTGAACATGCTAAAAGAAACGAGCTTTTCGATCGTATGGTGAGTATCAGAAGGAAAATCCATGAAAACCCAGAGCTTTGTTATGAGGAATTTGAAACCAGTAAGCTTATCAGAGCTGAACTTGACCGGCTGGGCATCCCTTACAGATACCCTTTGGCTGAAACCGGCGTTGTTGGCCTTGTTGGGACCGGTGGTCCACCCTTTGTTGCAATCAGAGCAGACATGGATGCTTTGGCTATGCAGGAGAGTGTGGAGTGGGAGCACAAGAGTAAAATTCCTGGGAAGATGCATGGATGTGGACATGATGCTCATGTTGCCATGCTTCTTGGAGCTGCAAGTATACTTCAAGAGTATCGCCACAAATTACAGGGGACTGTTGTTCTTATTTTCCAACCAGCTGAGGAAGGAGGCGCAGGAGCAAAGAAAATGATAGATGAAGGTGCAGTGGAGCATGTCAGTGCCATCTTTGGGTTACATGTCACTGCTGAGCACCCCATTGGTTCAGTGGCCACCAGGCCTGGCCCTATTTTGGCTGCGAGTGGATTTTTTGAAGCCGTAATAAGTGGGAAGGGGGGTCATGCTGCAATTCCACAGCACACAATAGACCCAATCTTGGCTGCTTCCAGTGTTATTGTTAGTCTGCAACATCTTGTTTCACGTGAAGCCGACCCCTTGGATTCACAGGTTGTGACTGTTGGAAAATTCCACGGAGGTGGTGCATTTAATGTTATTCCAGATTCTGTTACAATTGGTGGTACTTTCAGGGCATTTTCAAAGGACAGCTTTATCCAACTTAAACAAAGAATCGAAGAGGTTATTACTAGACAAGCCAGTGTTCAGAGGTGCAATGCAACTGTCTTATTCAATGAAATTGCACGGCCCTTTTACCCTGCAACTGTAAATGACAAAGATTTGCATGAGTACTTCCGGAAAGTTACAGTCGATTTGGTGGGCCCTCAGAATGTATTTGAGAAGCAACCACTGATGGGGGCGGAAGACTTCTCATTCTATCAAGAAGTAATTCCAGGGTACTTCTTCTTTCTTGGTATGAAGAATGACACTCAAGGACAGTTCAAACCAGCGCATTCACCATATTTCAGAGTAAACGAAGACGTGCTTCCTTATGGAGCTGCATTCCATGCATCACTGGCCGCCAGGTACCTCCTGGAAAAGCAACCGAAATCTACCTCAACAGCAGACCAAAAAATGAAATCCACTTCAATGAAAGGGAACTTTCATGATGAACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010179 GO:0010817 GO:0012505 GO:0016787 GO:0016810 GO:0042445 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

503

Amino Acids

55.51

Weight (kDa)

6.38

Isoelectric Point (pI)

37.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	157 - 472	5.2e-44	Peptidase family M20/M25/M40
M20_dimer	PF07687	271 - 363	5.7e-11	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010736)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56650 AT5G56650 AT5G56660
fragaria_vesca	FvH4_1g06730 FvH4_1g06730
malus_domestica	MD02G1072200.v1.1 MD15G1202000.v1.1
prunus_persica	Prupe.7G215300_v2.0.a1
pyrus_communis	pycom02g05620 pycom15g17930
rosa_chinensis	RchiOBHm_Chr2g0092471
rosa_laevigata	RLG00000016315
rosa_multiflora	Rmu_sc0004296.1_g000017 Rmu_sc0023787.1_g000002
rosa_roxburghii	Rroxscaffold_2G00149070
rosa_rugosa	Rorug02G0026900
rosa_samantha	Rh2AG072700 Rh2BG073200 Rh2CG074500
rosa_wichuraiana	Rw2G006020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1419
AccB1I	GGYRCC	1 cut(s)	1419
AccI	GTMKAC	1 cut(s)	601
AccIII	TCCGGA	1 cut(s)	1188
AciI	CCGC	3 cut(s)	21, 1253, 1413
AcoI	YGGCCR	2 cut(s)	768, 1410
AcsI	RAATTY	4 cut(s)	116, 350, 534, 962
AcvI	CACGTG	1 cut(s)	920
AfaI	GTAC	4 cut(s)	1018, 1184, 1292, 1421
AfiI	CCNNNNNNNGG	6 cut(s)	157, 232, 729, 790, 874, 1247
AflIII	ACRYGT	1 cut(s)	736
AgeI	ACCGGT	1 cut(s)	449
AhdI	GACNNNNNGTC	1 cut(s)	453
AjiI	CACGTC	1 cut(s)	1375
AjnI	CCWGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
AleI	CACNNNNGTG	1 cut(s)	972
AluBI	AGCT	9 cut(s)	238, 286, 337, 367, 377, 593, 659, 1044, 1391
AluI	AGCT	9 cut(s)	238, 286, 337, 367, 377, 593, 659, 1044, 1391
Alw21I	GWGCWC	2 cut(s)	525, 753
Alw26I	GTCTC	1 cut(s)	38
AlwNI	CAGNNNCTG	1 cut(s)	1001
Aor13HI	TCCGGA	1 cut(s)	1188
AoxI	GGCC	7 cut(s)	436, 768, 776, 781, 1139, 1213, 1410
ApaI	GGGCCC	1 cut(s)	1217
ApeKI	GCWGC	6 cut(s)	23, 593, 794, 842, 878, 1391
ApoI	RAATTY	4 cut(s)	116, 350, 534, 962
AsiGI	ACCGGT	1 cut(s)	449
Asp700I	GAANNNNTTC	1 cut(s)	1281
Asp718I	GGTACC	1 cut(s)	1419
AspLEI	GCGC	2 cut(s)	674, 1345
AspS9I	GGNCC	6 cut(s)	447, 455, 782, 1140, 1213, 1214
AsuHPI	GGTGA	3 cut(s)	9, 313, 1341
AvaII	GGWCC	2 cut(s)	447, 455
BaeGI	GKGCMC	1 cut(s)	1217
BalI	TGGCCA	1 cut(s)	770
BanI	GGYRCC	1 cut(s)	1419
BanII	GRGCYC	2 cut(s)	227, 1217
BbrPI	CACGTG	1 cut(s)	920
BbsI	GAAGAC	2 cut(s)	1263, 1377
Bbv12I	GWGCWC	2 cut(s)	525, 753
BbvCI	CCTCAGC	1 cut(s)	660
BbvI	GCAGC	6 cut(s)	10, 580, 781, 829, 865, 1378
BccI	CCATC	2 cut(s)	731, 1240
BceAI	ACGGC	2 cut(s)	800, 1154
BciT130I	CCWGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
BcoDI	GTCTC	1 cut(s)	38
BfaI	CTAG	1 cut(s)	1083
BisI	GCNGC	7 cut(s)	24, 594, 795, 843, 879, 1392, 1413
BlpI	GCTNAGC	1 cut(s)	747
BlsI	GCNGC	7 cut(s)	25, 595, 796, 844, 880, 1393, 1414
BmcAI	AGTACT	1 cut(s)	1184
Bme1390I	CCNGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
Bme18I	GGWCC	2 cut(s)	447, 455
BmeRI	GACNNNNNGTC	1 cut(s)	453
BmgBI	CACGTC	1 cut(s)	1375
BmgT120I	GGNCC	6 cut(s)	447, 455, 782, 1140, 1213, 1214
BmiI	GGNNCC	3 cut(s)	448, 1215, 1421
BmrFI	CCNGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
BmrI	ACTGGG	1 cut(s)	245
BmsI	GCATC	5 cut(s)	405, 478, 537, 556, 1412
BmuI	ACTGGG	1 cut(s)	245
BpiI	GAAGAC	2 cut(s)	1263, 1377
Bpu10I	CCTNAGC	1 cut(s)	660
Bpu1102I	GCTNAGC	1 cut(s)	747
BpuEI	CTTGAG	1 cut(s)	1308
BsaAI	YACGTR	1 cut(s)	920
BsaJI	CCNNGG	4 cut(s)	539, 933, 967, 1286
BsaWI	WCCGGW	2 cut(s)	449, 1188
Bsc4I	CCNNNNNNNGG	6 cut(s)	157, 232, 729, 790, 874, 1247
Bse118I	RCCGGY	3 cut(s)	387, 425, 449
Bse1I	ACTGG	5 cut(s)	251, 360, 885, 1092, 1413
Bse3DI	GCAATG	1 cut(s)	1114
BseAI	TCCGGA	1 cut(s)	1188
BseBI	CCWGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
BseDI	CCNNGG	4 cut(s)	539, 933, 967, 1286
BseGI	GGATG	3 cut(s)	396, 493, 560
BseLI	CCNNNNNNNGG	6 cut(s)	157, 232, 729, 790, 874, 1247
BseMI	GCAATG	1 cut(s)	1114
BseMII	CTCAG	3 cut(s)	651, 738, 1232
BseNI	ACTGG	5 cut(s)	251, 360, 885, 1092, 1413
BseSI	GKGCMC	1 cut(s)	1217
BseXI	GCAGC	6 cut(s)	10, 580, 781, 829, 865, 1378
BseYI	CCCAGC	1 cut(s)	391
BsgI	GTGCAG	1 cut(s)	723
Bsh1285I	CGRYCG	1 cut(s)	295
BshFI	GGCC	7 cut(s)	438, 770, 778, 783, 1141, 1215, 1412
BshNI	GGYRCC	1 cut(s)	1419
BshTI	ACCGGT	1 cut(s)	449
BsiEI	CGRYCG	1 cut(s)	295
BsiHKAI	GWGCWC	2 cut(s)	525, 753
BsiSI	CCGG	4 cut(s)	388, 426, 450, 1189
BslFI	GGGAC	4 cut(s)	26, 33, 460, 646
BslI	CCNNNNNNNGG	6 cut(s)	157, 232, 729, 790, 874, 1247
BsmAI	GTCTC	1 cut(s)	38
BsmBI	CGTCTC	1 cut(s)	38
BsmFI	GGGAC	4 cut(s)	26, 33, 460, 646
BsmI	GAATGC	2 cut(s)	1345, 1394
BsnI	GGCC	7 cut(s)	438, 770, 778, 783, 1141, 1215, 1412
Bsp120I	GGGCCC	1 cut(s)	1213
Bsp1286I	GDGCHC	4 cut(s)	227, 525, 753, 1217
Bsp13I	TCCGGA	1 cut(s)	1188
Bsp143I	GATC	1 cut(s)	292
Bsp1720I	GCTNAGC	1 cut(s)	747
BspACI	CCGC	3 cut(s)	21, 1253, 1413
BspANI	GGCC	7 cut(s)	438, 770, 778, 783, 1141, 1215, 1412
BspCNI	CTCAG	3 cut(s)	652, 739, 1231
BspEI	TCCGGA	1 cut(s)	1188
BspHI	TCATGA	1 cut(s)	1498
BspLI	GGNNCC	3 cut(s)	448, 1215, 1421
BspT107I	GGYRCC	1 cut(s)	1419
BsrDI	GCAATG	1 cut(s)	1114
BsrFI	RCCGGY	3 cut(s)	387, 425, 449
BsrI	ACTGG	5 cut(s)	251, 360, 885, 1092, 1413
BssAI	RCCGGY	3 cut(s)	387, 425, 449
BssECI	CCNNGG	4 cut(s)	539, 933, 967, 1286
BssMI	GATC	1 cut(s)	292
BssNAI	GTATAC	1 cut(s)	602
BssT1I	CCWWGG	1 cut(s)	933
Bst1107I	GTATAC	1 cut(s)	602
Bst2UI	CCWGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
Bst4CI	ACNGT	6 cut(s)	637, 955, 1117, 1159, 1201, 1332
Bst6I	CTCTTC	1 cut(s)	1065
BstAPI	GCANNNNNTGC	1 cut(s)	1400
BstBAI	YACGTR	1 cut(s)	920
BstC8I	GCNNGC	1 cut(s)	240
BstDEI	CTNAG	3 cut(s)	660, 747, 1218
BstDSI	CCRYGG	1 cut(s)	967
BstF5I	GGATG	3 cut(s)	396, 493, 560
BstHHI	GCGC	2 cut(s)	674, 1345
BstKTI	GATC	1 cut(s)	295
BstMAI	GTCTC	1 cut(s)	38
BstMBI	GATC	1 cut(s)	292
BstMCI	CGRYCG	1 cut(s)	295
BstMWI	GCNNNNNNNGC	5 cut(s)	435, 476, 575, 1400, 1409
BstNI	CCWGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
BstNSI	RCATGY	3 cut(s)	272, 716, 740
BstSCI	CCNGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
BstSLI	GKGCMC	1 cut(s)	1217
BstV1I	GCAGC	6 cut(s)	10, 580, 781, 829, 865, 1378
BstV2I	GAAGAC	2 cut(s)	1263, 1377
BstXI	CCANNNNNNTGG	1 cut(s)	974
BstZ17I	GTATAC	1 cut(s)	602
BsuRI	GGCC	7 cut(s)	438, 770, 778, 783, 1141, 1215, 1412
BtgI	CCRYGG	1 cut(s)	967
BtrI	CACGTC	1 cut(s)	1375
BtsCI	GGATG	3 cut(s)	396, 493, 560
BtsI	GCAGTG	3 cut(s)	194, 711, 741
BtsIMutI	CAGTG	9 cut(s)	194, 711, 724, 741, 771, 892, 1099, 1241, 1406
Cac8I	GCNNGC	1 cut(s)	240
CaiI	CAGNNNCTG	1 cut(s)	1001
CciI	TCATGA	1 cut(s)	1498
CfoI	GCGC	2 cut(s)	674, 1345
Cfr10I	RCCGGY	3 cut(s)	387, 425, 449
Cfr13I	GGNCC	6 cut(s)	447, 455, 782, 1140, 1213, 1214
Csp6I	GTAC	4 cut(s)	1017, 1183, 1291, 1420
CspAI	ACCGGT	1 cut(s)	449
CviQI	GTAC	4 cut(s)	1017, 1183, 1291, 1420
DdeI	CTNAG	3 cut(s)	660, 747, 1218
DpnI	GATC	1 cut(s)	294
DpnII	GATC	1 cut(s)	292
DriI	GACNNNNNGTC	1 cut(s)	453
EaeI	YGGCCR	2 cut(s)	768, 1410
Eam1104I	CTCTTC	1 cut(s)	1065
Eam1105I	GACNNNNNGTC	1 cut(s)	453
EarI	CTCTTC	1 cut(s)	1065
EciI	GGCGGA	1 cut(s)	1268
Eco130I	CCWWGG	1 cut(s)	933
Eco147I	AGGCCT	1 cut(s)	778
Eco24I	GRGCYC	2 cut(s)	227, 1217
Eco47I	GGWCC	2 cut(s)	447, 455
Eco72I	CACGTG	1 cut(s)	920
EcoO109I	RGGNCCY	1 cut(s)	1214
EcoRII	CCWGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
EcoT14I	CCWWGG	1 cut(s)	933
EcoT22I	ATGCAT	2 cut(s)	552, 1405
EcoT38I	GRGCYC	2 cut(s)	227, 1217
ErhI	CCWWGG	1 cut(s)	933
Esp3I	CGTCTC	1 cut(s)	38
FalI	AAGNNNNNCTT	4 cut(s)	1028, 1060, 1362, 1394
FaqI	GGGAC	4 cut(s)	26, 33, 460, 646
FauI	CCCGC	1 cut(s)	28
FblI	GTMKAC	1 cut(s)	601
Fnu4HI	GCNGC	7 cut(s)	24, 594, 795, 843, 879, 1392, 1413
FokI	GGATG	3 cut(s)	383, 500, 567
FriOI	GRGCYC	2 cut(s)	227, 1217
Fsp4HI	GCNGC	7 cut(s)	24, 594, 795, 843, 879, 1392, 1413
FspBI	CTAG	1 cut(s)	1083
GlaI	GCGC	2 cut(s)	673, 1344
GluI	GCNGC	7 cut(s)	24, 594, 795, 843, 879, 1392, 1413
GsaI	CCCAGC	1 cut(s)	395
HaeIII	GGCC	7 cut(s)	438, 770, 778, 783, 1141, 1215, 1412
HapII	CCGG	4 cut(s)	388, 426, 450, 1189
HhaI	GCGC	2 cut(s)	674, 1345
Hin6I	GCGC	2 cut(s)	672, 1343
HinP1I	GCGC	2 cut(s)	672, 1343
HindIII	AAGCTT	1 cut(s)	365
HpaII	CCGG	4 cut(s)	388, 426, 450, 1189
HphI	GGTGA	3 cut(s)	9, 313, 1341
Hpy166II	GTNNAC	4 cut(s)	458, 560, 602, 1366
Hpy188III	TCNNGA	8 cut(s)	7, 112, 263, 608, 995, 1189, 1274, 1499
Hpy8I	GTNNAC	4 cut(s)	458, 560, 602, 1366
HpyAV	CCTTC	5 cut(s)	113, 306, 659, 692, 823
HpyCH4III	ACNGT	6 cut(s)	637, 955, 1117, 1159, 1201, 1332
HpyCH4IV	ACGT	2 cut(s)	919, 1374
HpyF10VI	GCNNNNNNNGC	5 cut(s)	435, 476, 575, 1400, 1409
HpyF3I	CTNAG	3 cut(s)	660, 747, 1218
HpySE526I	ACGT	2 cut(s)	919, 1374
HspAI	GCGC	2 cut(s)	672, 1343
Kpn2I	TCCGGA	1 cut(s)	1188
KpnI	GGTACC	1 cut(s)	1423
Kzo9I	GATC	1 cut(s)	292
LmnI	GCTCC	6 cut(s)	235, 520, 590, 677, 709, 1388
Lsp1109I	GCAGC	6 cut(s)	10, 580, 781, 829, 865, 1378
LweI	GCATC	5 cut(s)	405, 478, 537, 556, 1412
MaeI	CTAG	1 cut(s)	1083
MaeII	ACGT	2 cut(s)	919, 1374
MaeIII	GTNAC	6 cut(s)	4, 732, 739, 949, 1003, 1195
MalI	GATC	1 cut(s)	294
MboI	GATC	1 cut(s)	292
MboII	GAAGA	7 cut(s)	210, 556, 1082, 1268, 1288, 1324, 1382
MfeI	CAATTG	1 cut(s)	1008
MhlI	GDGCHC	4 cut(s)	227, 525, 753, 1217
MlsI	TGGCCA	1 cut(s)	770
MluCI	AATT	9 cut(s)	116, 350, 534, 623, 846, 962, 1008, 1131, 1281
MluNI	TGGCCA	1 cut(s)	770
MlyI	GAGTC	2 cut(s)	4, 48
MmeI	TCCRAC	3 cut(s)	676, 937, 1075
Mox20I	TGGCCA	1 cut(s)	770
Mph1103I	ATGCAT	2 cut(s)	552, 1405
MroI	TCCGGA	1 cut(s)	1188
MroXI	GAANNNNTTC	1 cut(s)	1281
MscI	TGGCCA	1 cut(s)	770
MseI	TTAA	3 cut(s)	134, 984, 1056
MslI	CAYNNNNRTG	4 cut(s)	555, 717, 972, 1481
Msp20I	TGGCCA	1 cut(s)	770
MspA1I	CMGCKG	2 cut(s)	23, 659
MspI	CCGG	4 cut(s)	388, 426, 450, 1189
MspR9I	CCNGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
MunI	CAATTG	1 cut(s)	1008
Mva1269I	GAATGC	2 cut(s)	1345, 1394
MvaI	CCWGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
MwoI	GCNNNNNNNGC	5 cut(s)	435, 476, 575, 1400, 1409
NdeII	GATC	1 cut(s)	292
NlaIV	GGNNCC	3 cut(s)	448, 1215, 1421
NmuCI	GTSAC	3 cut(s)	4, 739, 949
NsiI	ATGCAT	2 cut(s)	552, 1405
NspI	RCATGY	3 cut(s)	272, 716, 740
OliI	CACNNNNGTG	1 cut(s)	972
PagI	TCATGA	1 cut(s)	1498
PceI	AGGCCT	1 cut(s)	778
PciI	ACATGT	1 cut(s)	736
PcsI	WCGNNNNNNNCGW	1 cut(s)	288
PctI	GAATGC	2 cut(s)	1345, 1394
PdmI	GAANNNNTTC	1 cut(s)	1281
PfeI	GAWTC	8 cut(s)	58, 64, 70, 76, 82, 938, 998, 1065
PfoI	TCCNGGA	1 cut(s)	1425
PinAI	ACCGGT	1 cut(s)	449
PkrI	GCNGC	7 cut(s)	25, 595, 796, 844, 880, 1393, 1414
Ple19I	CGATCG	1 cut(s)	295
PleI	GAGTC	2 cut(s)	4, 47
PmaCI	CACGTG	1 cut(s)	920
PmlI	CACGTG	1 cut(s)	920
PpsI	GAGTC	2 cut(s)	4, 47
Ppu21I	YACGTR	1 cut(s)	920
PscI	ACATGT	1 cut(s)	736
Psp6I	CCWGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
PspCI	CACGTG	1 cut(s)	920
PspFI	CCCAGC	1 cut(s)	391
PspGI	CCWGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
PspN4I	GGNNCC	3 cut(s)	448, 1215, 1421
PspOMI	GGGCCC	1 cut(s)	1213
PspPI	GGNCC	6 cut(s)	447, 455, 782, 1140, 1213, 1214
PstNI	CAGNNNCTG	1 cut(s)	1001
PvuI	CGATCG	1 cut(s)	295
PvuII	CAGCTG	1 cut(s)	659
RsaI	GTAC	4 cut(s)	1018, 1184, 1292, 1421
RsaNI	GTAC	4 cut(s)	1017, 1183, 1291, 1420
RseI	CAYNNNNRTG	4 cut(s)	555, 717, 972, 1481
SaqAI	TTAA	3 cut(s)	134, 984, 1056
SatI	GCNGC	7 cut(s)	24, 594, 795, 843, 879, 1392, 1413
Sau3AI	GATC	1 cut(s)	292
Sau96I	GGNCC	6 cut(s)	447, 455, 782, 1140, 1213, 1214
ScaI	AGTACT	1 cut(s)	1184
SchI	GAGTC	2 cut(s)	4, 48
ScrFI	CCNGG	6 cut(s)	540, 775, 780, 1287, 1417, 1427
SduI	GDGCHC	4 cut(s)	227, 525, 753, 1217
SfaNI	GCATC	5 cut(s)	405, 478, 537, 556, 1412
SinI	GGWCC	2 cut(s)	447, 455
SmiMI	CAYNNNNRTG	4 cut(s)	555, 717, 972, 1481
SmlI	CTYRAG	1 cut(s)	1323
SmoI	CTYRAG	1 cut(s)	1323
Sse9I	AATT	9 cut(s)	116, 350, 534, 623, 846, 962, 1008, 1131, 1281
SseBI	AGGCCT	1 cut(s)	778
SsiI	CCGC	3 cut(s)	21, 1253, 1413
SspMI	CTAG	1 cut(s)	1083
StuI	AGGCCT	1 cut(s)	778
StyD4I	CCNGG	6 cut(s)	538, 773, 778, 1285, 1415, 1425
StyI	CCWWGG	1 cut(s)	933
TaaI	ACNGT	6 cut(s)	637, 955, 1117, 1159, 1201, 1332
TaiI	ACGT	2 cut(s)	922, 1377
TaqI	TCGA	4 cut(s)	221, 291, 1068, 1203
TasI	AATT	9 cut(s)	116, 350, 534, 623, 846, 962, 1008, 1131, 1281
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	1 cut(s)	1415
TfiI	GAWTC	8 cut(s)	58, 64, 70, 76, 82, 938, 998, 1065
Tru1I	TTAA	3 cut(s)	134, 984, 1056
Tru9I	TTAA	3 cut(s)	134, 984, 1056
TscAI	CASTG	9 cut(s)	201, 711, 724, 748, 771, 892, 1099, 1248, 1413
TseFI	GTSAC	3 cut(s)	4, 739, 949
TseI	GCWGC	6 cut(s)	23, 593, 794, 842, 878, 1391
Tsp45I	GTSAC	3 cut(s)	4, 739, 949
TspDTI	ATGAA	9 cut(s)	117, 163, 339, 711, 1143, 1325, 1484, 1487, 1499
TspGWI	ACGGA	1 cut(s)	984
TspRI	CASTG	9 cut(s)	201, 711, 724, 748, 771, 892, 1099, 1248, 1413
VpaK11BI	GGWCC	2 cut(s)	447, 455
XapI	RAATTY	4 cut(s)	116, 350, 534, 962
XceI	RCATGY	3 cut(s)	272, 716, 740
XcmI	CCANNNNNNNNNTGG	2 cut(s)	763, 1423
XmiI	GTMKAC	1 cut(s)	601
XmnI	GAANNNNTTC	1 cut(s)	1281
XspI	CTAG	1 cut(s)	1083
ZrmI	AGTACT	1 cut(s)	1184
Zsp2I	ATGCAT	2 cut(s)	552, 1405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.