AT5G57070

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012501
Physical Location & Seq
Forward (+)
23096024 .. 23098602
2579 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57070.1

Sequence Viewer

Length: 1728 bp
ATGGACGATCAACCGCCGCTGATCTGGCCCCAGTTTGACTCTACCGGATATGCTCGCCGGAGATCATCGATTCCGGCGATTTTAGTTCCGGCGATGATCGGAGTAACTTCAGCGGCGATTTTTCTTGTCTTTGTGACCTTCGTCGTTCCTACGTTTCTCTCAGTCACATCTCAGATTCTTCAGCCAGCTTCGGTGAAGCGAGGATGGGATTCGATAAACGTGGTTTTAGTGGTTTTCGCGATTCTCTGCGGCGTACTCGCTAGACGAAACGACGACGGATTGTCTTCGGAGTCTTTACACGGCGGAGAAGAAGAAGAAGTAGGAGGAGGAGCCGTTACGAATGGTGAAATGACCGTCGGTGAGATTAGCAAAATCTCATCCTCGTCGTCGACGGTTTCAGAGCAGTGGTTTGATGACGTGTATGATTCTGATAGGCTTAAGATCTATGAGAGTGTAAGTAGTCGAAGTTTTTCTCATGGATTACCGGTTACCGGGAATGTACCGTTGAGGCGTAGTAGTAGCTCTTATCCTGATCTCAGACAAGGAGTTTTTAGAGAAACTGGCGATCGACGGTTCCGGTTTTATGATGATTTCGAAATCGATAAGTACCGATCGCAGGATTCGAGTTCTTACCAACAATTTCAGAATCTTTCTAAAACTGAAATCGAGGAAGAAGAATCTGAGCCTAAAGAGATTCAGATTGATACGTTCGTTGTAAAACCGTCTTCTCCGCCGCAACAACCACCGGCAACACCACCACCTCCTCCCCCACCTCCGCCGGTTGAAGTTCCGCAGAAACCGAGAAGGACTCATCGTTCTGTTAGAAACAGAGATTTACAAGAAAACGCCAAACGCAGTGAGACTAAGTTTAAACGAACGTTTCAGCCTCCGCCGTCACCACCGCCTCCACCGCCCCCACCACCTCCTCAGCCGCTTATAGCCGCTACGCCACCAAGAAAACAAGGAACTCTTCAAAGAAGAAAGAGCAATGCGGCGAAAGAGATTAAAATGGTTTTCGCTTCTCTGTATAATCAAGGGAAGAAGAAGAAGAAGCTTCAGAAATCGAAGAGAAAAGAGAGAATCGAATCTTCTCCGATGGTGGAAGATGTAACGGAACCACCGCAATACCAATCGTTAATCCCACCACCGTCTCCGCCTCCTCCTCCACCACCACCTCCGCCTCCATTGCGATCGTCACAATCAGTATTCTACGGATTGTTCAAGAAAGGAGTTAAGAGCAACAAAAAGATTCATTCCGTTCCGGCGCCGCCTCCACCTCCACCGCCGAGATATACCCAATTTGATCCTCAGACACCACCACGGAGAGTGAAATCCGGAAGACCTCCGCGTCCGACGAAACCGAAGAATTTCAATGAAGAGAACAACGGCCAAGGATCTCCGTTAATCCAAATTACGCCTCCACCTCCACCACCGCCTCCGTTTAGAGTTCCGCCGCTAAAATATGTCGTGAGTGGTGATTTCGCGAAGATACGGAGTAATCAGAGCTCTAGATGTAGCTCCCCTGAACGGGAAGTGTTCGATATCGGTTGGGGTCTAGAACTCACACAATCTGATGGCGGAGTTGAGACTAAAGCTGCCGTCAGCGGCGGCGGTATGCCGGGATTCTGCCCAAGCCCGGACGTTGATACGAAAGCCGACAATTTCATTGCCAGGCTTAGAGATGAGTGGAGGCTCGATAAGATCAATTCCGTGAATAGAAAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04062 ko04144 ko04520 ko04530 ko04666 ko04810 ko05100 ko05130 ko05131 ko05132 ko05231 map04062 map04144 map04520 map04530 map04666 map04810 map05100 map05130 map05131 map05132 map05231
Pfam Domains
Protein Families
DUF761

Protein Analysis

575

Amino Acids

63.85

Weight (kDa)

9.66

Isoelectric Point (pI)

87.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1347
AccB1I GGYRCC 1 cut(s) 1264
AccI GTMKAC 1 cut(s) 389
AccII CGCG 3 cut(s) 239, 1348, 1484
AccIII TCCGGA 1 cut(s) 1334
AclI AACGTT 1 cut(s) 878
AclWI GGATC 2 cut(s) 1298, 1402
AcoI YGGCCR 1 cut(s) 1387
AcsI RAATTY 1 cut(s) 1366
AcuI CTGAAG 3 cut(s) 93, 164, 1040
AcyI GRCGYC 1 cut(s) 1265
AfaI GTAC 3 cut(s) 255, 501, 608
AfiI CCNNNNNNNGG 5 cut(s) 491, 616, 1527, 1528, 1636
AflII CTTAAG 1 cut(s) 437
AflIII ACRYGT 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 484
AgsI TTSAA 4 cut(s) 785, 974, 1222, 1372
AhdI GACNNNNNGTC 1 cut(s) 280
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 1 cut(s) 1670
AloI GAACNNNNNNTCC 2 cut(s) 799, 831
AluBI AGCT 6 cut(s) 188, 522, 1054, 1506, 1518, 1595
AluI AGCT 6 cut(s) 188, 522, 1054, 1506, 1518, 1595
Alw21I GWGCWC 1 cut(s) 1508
Alw26I GTCTC 3 cut(s) 854, 1155, 1580
AlwI GGATC 2 cut(s) 1298, 1402
Aor13HI TCCGGA 1 cut(s) 1334
AoxI GGCC 2 cut(s) 26, 1387
ApeKI GCWGC 1 cut(s) 1595
ApoI RAATTY 1 cut(s) 1366
AsiGI ACCGGT 1 cut(s) 484
Asp700I GAANNNNTTC 2 cut(s) 469, 1367
AspLEI GCGC 1 cut(s) 1267
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 3 cut(s) 493, 1620, 1637
AsuHPI GGTGA 5 cut(s) 205, 356, 371, 888, 1487
AsuII TTCGAA 1 cut(s) 594
BaeI ACNNNNGTAYC 2 cut(s) 696, 729
BanI GGYRCC 1 cut(s) 1264
BanII GRGCYC 1 cut(s) 1508
BbsI GAAGAC 3 cut(s) 276, 717, 1345
Bbv12I GWGCWC 1 cut(s) 1508
BbvCI CCTCAGC 1 cut(s) 927
BbvI GCAGC 1 cut(s) 1582
BccI CCATC 3 cut(s) 198, 1090, 1568
BceAI ACGGC 5 cut(s) 316, 317, 877, 1402, 1583
BciT130I CCWGG 1 cut(s) 1672
BcnI CCSGG 3 cut(s) 493, 1620, 1637
BcoDI GTCTC 3 cut(s) 854, 1155, 1580
BfaI CTAG 3 cut(s) 261, 1509, 1556
BfoI RGCGCY 1 cut(s) 1268
BfrI CTTAAG 1 cut(s) 437
BglII AGATCT 1 cut(s) 441
Bme1390I CCNGG 4 cut(s) 493, 1620, 1637, 1672
BmeRI GACNNNNNGTC 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 5 cut(s) 29, 331, 575, 1116, 1266
BmrFI CCNGG 4 cut(s) 493, 1620, 1637, 1672
BmrI ACTGGG 1 cut(s) 25
BmuI ACTGGG 1 cut(s) 25
BoxI GACNNNNGTC 1 cut(s) 140
BpiI GAAGAC 3 cut(s) 276, 717, 1345
BplI GAGNNNNNCTC 2 cut(s) 793, 825
Bpu10I CCTNAGC 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 594
BpuMI CCSGG 3 cut(s) 493, 1620, 1637
Bsa29I ATCGAT 2 cut(s) 68, 600
BsaHI GRCGYC 1 cut(s) 1265
BsaJI CCNNGG 2 cut(s) 1319, 1390
BsaWI WCCGGW 4 cut(s) 44, 484, 576, 1334
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 5 cut(s) 491, 616, 1527, 1528, 1636
Bse118I RCCGGY 3 cut(s) 484, 745, 778
Bse1I ACTGG 2 cut(s) 31, 565
Bse3DI GCAATG 3 cut(s) 994, 1184, 1665
BseAI TCCGGA 1 cut(s) 1334
BseBI CCWGG 1 cut(s) 1672
BseCI ATCGAT 2 cut(s) 68, 600
BseDI CCNNGG 2 cut(s) 1319, 1390
BseGI GGATG 2 cut(s) 209, 377
BseLI CCNNNNNNNGG 5 cut(s) 491, 616, 1527, 1528, 1636
BseMI GCAATG 3 cut(s) 994, 1184, 1665
BseMII CTCAG 6 cut(s) 174, 185, 550, 672, 941, 1322
BseNI ACTGG 2 cut(s) 31, 565
BseRI GAGGAG 6 cut(s) 339, 342, 753, 915, 1149, 1152
BseXI GCAGC 1 cut(s) 1582
Bsh1236I CGCG 3 cut(s) 239, 1348, 1484
Bsh1285I CGRYCG 3 cut(s) 568, 614, 1193
BshFI GGCC 2 cut(s) 28, 1389
BshNI GGYRCC 1 cut(s) 1264
BshTI ACCGGT 1 cut(s) 484
BshVI ATCGAT 2 cut(s) 68, 600
BsiEI CGRYCG 3 cut(s) 568, 614, 1193
BsiHKAI GWGCWC 1 cut(s) 1508
BslI CCNNNNNNNGG 5 cut(s) 491, 616, 1527, 1528, 1636
BsmAI GTCTC 3 cut(s) 854, 1155, 1580
BsmBI CGTCTC 1 cut(s) 1155
BsnI GGCC 2 cut(s) 28, 1389
Bsp119I TTCGAA 1 cut(s) 594
Bsp1286I GDGCHC 1 cut(s) 1508
Bsp13I TCCGGA 1 cut(s) 1334
Bsp68I TCGCGA 2 cut(s) 239, 1484
BspANI GGCC 2 cut(s) 28, 1389
BspCNI CTCAG 6 cut(s) 173, 184, 549, 673, 940, 1321
BspDI ATCGAT 2 cut(s) 68, 600
BspEI TCCGGA 1 cut(s) 1334
BspFNI CGCG 3 cut(s) 239, 1348, 1484
BspLI GGNNCC 5 cut(s) 29, 331, 575, 1116, 1266
BspPI GGATC 2 cut(s) 1298, 1402
BspT104I TTCGAA 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 1264
BspTI CTTAAG 1 cut(s) 437
BsrDI GCAATG 3 cut(s) 994, 1184, 1665
BsrFI RCCGGY 3 cut(s) 484, 745, 778
BsrI ACTGG 2 cut(s) 31, 565
BssAI RCCGGY 3 cut(s) 484, 745, 778
BssECI CCNNGG 2 cut(s) 1319, 1390
BssNI GRCGYC 1 cut(s) 1265
BssT1I CCWWGG 1 cut(s) 1390
Bst2UI CCWGG 1 cut(s) 1672
Bst4CI ACNGT 6 cut(s) 355, 394, 504, 573, 723, 1149
Bst6I CTCTTC 3 cut(s) 975, 1061, 1371
BstACI GRCGYC 1 cut(s) 1265
BstAFI CTTAAG 1 cut(s) 437
BstBI TTCGAA 1 cut(s) 594
BstC8I GCNNGC 2 cut(s) 55, 186
BstDEI CTNAG 8 cut(s) 160, 171, 536, 681, 864, 927, 1308, 1676
BstDSI CCRYGG 1 cut(s) 1319
BstEII GGTNACC 1 cut(s) 487
BstF5I GGATG 2 cut(s) 209, 377
BstFNI CGCG 3 cut(s) 239, 1348, 1484
BstH2I RGCGCY 1 cut(s) 1268
BstHHI GCGC 1 cut(s) 1267
BstMAI GTCTC 3 cut(s) 854, 1155, 1580
BstMCI CGRYCG 3 cut(s) 568, 614, 1193
BstMWI GCNNNNNNNGC 3 cut(s) 25, 910, 1186
BstNI CCWGG 1 cut(s) 1672
BstPAI GACNNNNGTC 1 cut(s) 140
BstPI GGTNACC 1 cut(s) 487
BstSCI CCNGG 4 cut(s) 491, 1618, 1635, 1670
BstUI CGCG 3 cut(s) 239, 1348, 1484
BstV1I GCAGC 1 cut(s) 1582
BstV2I GAAGAC 3 cut(s) 276, 717, 1345
BstX2I RGATCY 2 cut(s) 441, 1394
BstYI RGATCY 2 cut(s) 441, 1394
Bsu15I ATCGAT 2 cut(s) 68, 600
BsuRI GGCC 2 cut(s) 28, 1389
BsuTUI ATCGAT 2 cut(s) 68, 600
BtgI CCRYGG 1 cut(s) 1319
BtgZI GCGATG 1 cut(s) 107
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 2 cut(s) 209, 377
BtsI GCAGTG 2 cut(s) 410, 862
BtsIMutI CAGTG 2 cut(s) 410, 862
BtuMI TCGCGA 2 cut(s) 239, 1484
Cac8I GCNNGC 2 cut(s) 55, 186
CfoI GCGC 1 cut(s) 1267
Cfr10I RCCGGY 3 cut(s) 484, 745, 778
Cfr13I GGNCC 1 cut(s) 27
ClaI ATCGAT 2 cut(s) 68, 600
CseI GACGC 1 cut(s) 1337
Csp6I GTAC 3 cut(s) 254, 500, 607
CspAI ACCGGT 1 cut(s) 484
CviAII CATG 1 cut(s) 476
CviQI GTAC 3 cut(s) 254, 500, 607
DdeI CTNAG 8 cut(s) 160, 171, 536, 681, 864, 927, 1308, 1676
DinI GGCGCC 1 cut(s) 1266
DraI TTTAAA 1 cut(s) 871
DrdI GACNNNNNNGTC 1 cut(s) 1347
DriI GACNNNNNGTC 1 cut(s) 280
DseDI GACNNNNNNGTC 1 cut(s) 1347
EaeI YGGCCR 1 cut(s) 1387
Eam1104I CTCTTC 3 cut(s) 975, 1061, 1371
Eam1105I GACNNNNNGTC 1 cut(s) 280
EarI CTCTTC 3 cut(s) 975, 1061, 1371
EciI GGCGGA 8 cut(s) 318, 720, 765, 879, 1143, 1167, 1440, 1593
Ecl136II GAGCTC 1 cut(s) 1506
Eco130I CCWWGG 1 cut(s) 1390
Eco24I GRGCYC 1 cut(s) 1508
Eco32I GATATC 1 cut(s) 1543
Eco53kI GAGCTC 1 cut(s) 1506
Eco57I CTGAAG 3 cut(s) 93, 164, 1040
Eco91I GGTNACC 1 cut(s) 487
EcoICRI GAGCTC 1 cut(s) 1506
EcoO65I GGTNACC 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 1670
EcoRV GATATC 1 cut(s) 1543
EcoT14I CCWWGG 1 cut(s) 1390
EcoT38I GRGCYC 1 cut(s) 1508
EgeI GGCGCC 1 cut(s) 1266
EheI GGCGCC 1 cut(s) 1266
ErhI CCWWGG 1 cut(s) 1390
Esp3I CGTCTC 1 cut(s) 1155
FaeI CATG 1 cut(s) 479
FalI AAGNNNNNCTT 2 cut(s) 954, 986
FatI CATG 1 cut(s) 475
FblI GTMKAC 1 cut(s) 389
FokI GGATG 2 cut(s) 216, 364
FriOI GRGCYC 1 cut(s) 1508
FspBI CTAG 3 cut(s) 261, 1509, 1556
GlaI GCGC 1 cut(s) 1266
HaeII RGCGCY 1 cut(s) 1268
HaeIII GGCC 2 cut(s) 28, 1389
HgaI GACGC 1 cut(s) 1337
HhaI GCGC 1 cut(s) 1267
Hin1I GRCGYC 1 cut(s) 1265
Hin1II CATG 1 cut(s) 479
Hin6I GCGC 1 cut(s) 1265
HinP1I GCGC 1 cut(s) 1265
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HindIII AAGCTT 1 cut(s) 1052
HphI GGTGA 5 cut(s) 205, 356, 371, 888, 1487
Hpy166II GTNNAC 1 cut(s) 390
Hpy188III TCNNGA 8 cut(s) 238, 530, 1222, 1335, 1468, 1483, 1509, 1556
Hpy8I GTNNAC 1 cut(s) 390
Hpy99I CGWCG 9 cut(s) 146, 275, 278, 359, 388, 391, 394, 573, 1357
HpyAV CCTTC 2 cut(s) 148, 798
HpyCH4III ACNGT 6 cut(s) 355, 394, 504, 573, 723, 1149
HpyCH4IV ACGT 6 cut(s) 152, 219, 417, 707, 878, 1641
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 910, 1186
HpyF3I CTNAG 8 cut(s) 160, 171, 536, 681, 864, 927, 1308, 1676
HpySE526I ACGT 6 cut(s) 152, 219, 417, 707, 878, 1641
Hsp92I GRCGYC 1 cut(s) 1265
Hsp92II CATG 1 cut(s) 479
HspAI GCGC 1 cut(s) 1265
KasI GGCGCC 1 cut(s) 1264
Kpn2I TCCGGA 1 cut(s) 1334
LmnI GCTCC 2 cut(s) 329, 1523
Lsp1109I GCAGC 1 cut(s) 1582
MaeI CTAG 3 cut(s) 261, 1509, 1556
MaeII ACGT 6 cut(s) 152, 219, 417, 707, 878, 1641
MaeIII GTNAC 8 cut(s) 103, 133, 163, 334, 487, 894, 1108, 1194
MflI RGATCY 2 cut(s) 441, 1394
MhlI GDGCHC 1 cut(s) 1508
MluCI AATT 6 cut(s) 638, 1298, 1366, 1410, 1660, 1705
Mly113I GGCGCC 1 cut(s) 1265
MlyI GAGTC 3 cut(s) 32, 299, 802
MmeI TCCRAC 1 cut(s) 1376
MroI TCCGGA 1 cut(s) 1334
MroXI GAANNNNTTC 2 cut(s) 469, 1367
MseI TTAA 6 cut(s) 438, 870, 1005, 1136, 1233, 1403
MspA1I CMGCKG 3 cut(s) 19, 113, 1605
MspCI CTTAAG 1 cut(s) 437
MspR9I CCNGG 4 cut(s) 493, 1620, 1637, 1672
MssI GTTTAAAC 1 cut(s) 871
MvaI CCWGG 1 cut(s) 1672
MvnI CGCG 3 cut(s) 239, 1348, 1484
MwoI GCNNNNNNNGC 3 cut(s) 25, 910, 1186
NarI GGCGCC 1 cut(s) 1265
NciI CCSGG 3 cut(s) 493, 1620, 1637
NlaIII CATG 1 cut(s) 479
NlaIV GGNNCC 5 cut(s) 29, 331, 575, 1116, 1266
NmeAIII GCCGAG 1 cut(s) 1311
NmuCI GTSAC 4 cut(s) 133, 163, 894, 1194
NruI TCGCGA 2 cut(s) 239, 1484
NspV TTCGAA 1 cut(s) 594
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 620
PdmI GAANNNNTTC 2 cut(s) 469, 1367
PinAI ACCGGT 1 cut(s) 484
Ple19I CGATCG 3 cut(s) 568, 614, 1193
PleI GAGTC 3 cut(s) 32, 298, 802
PluTI GGCGCC 1 cut(s) 1268
PmeI GTTTAAAC 1 cut(s) 871
PpsI GAGTC 3 cut(s) 32, 298, 802
PshAI GACNNNNGTC 1 cut(s) 140
Psp124BI GAGCTC 1 cut(s) 1508
Psp1406I AACGTT 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 1670
PspEI GGTNACC 1 cut(s) 487
PspGI CCWGG 1 cut(s) 1670
PspN4I GGNNCC 5 cut(s) 29, 331, 575, 1116, 1266
PspPI GGNCC 1 cut(s) 27
PsuI RGATCY 2 cut(s) 441, 1394
PvuI CGATCG 3 cut(s) 568, 614, 1193
RruI TCGCGA 2 cut(s) 239, 1484
RsaI GTAC 3 cut(s) 255, 501, 608
RsaNI GTAC 3 cut(s) 254, 500, 607
SacI GAGCTC 1 cut(s) 1508
SalI GTCGAC 1 cut(s) 388
SaqAI TTAA 6 cut(s) 438, 870, 1005, 1136, 1233, 1403
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 3 cut(s) 32, 299, 802
ScrFI CCNGG 4 cut(s) 493, 1620, 1637, 1672
SduI GDGCHC 1 cut(s) 1508
SfoI GGCGCC 1 cut(s) 1266
SfuI TTCGAA 1 cut(s) 594
SgrDI CGTCGACG 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 6 cut(s) 638, 1298, 1366, 1410, 1660, 1705
SspDI GGCGCC 1 cut(s) 1264
SspMI CTAG 3 cut(s) 261, 1509, 1556
SstI GAGCTC 1 cut(s) 1508
StyD4I CCNGG 4 cut(s) 491, 1618, 1635, 1670
StyI CCWWGG 1 cut(s) 1390
TaaI ACNGT 6 cut(s) 355, 394, 504, 573, 723, 1149
TaiI ACGT 6 cut(s) 155, 222, 420, 710, 881, 1644
TasI AATT 6 cut(s) 638, 1298, 1366, 1410, 1660, 1705
Tru1I TTAA 6 cut(s) 438, 870, 1005, 1136, 1233, 1403
Tru9I TTAA 6 cut(s) 438, 870, 1005, 1136, 1233, 1403
TscAI CASTG 2 cut(s) 410, 862
TseFI GTSAC 4 cut(s) 133, 163, 894, 1194
TseI GCWGC 1 cut(s) 1595
Tsp45I GTSAC 4 cut(s) 133, 163, 894, 1194
TspDTI ATGAA 3 cut(s) 1241, 1389, 1654
TspGWI ACGGA 9 cut(s) 291, 1127, 1227, 1246, 1336, 1389, 1428, 1507, 1699
TspRI CASTG 2 cut(s) 410, 862
Vha464I CTTAAG 1 cut(s) 437
XapI RAATTY 1 cut(s) 1366
XbaI TCTAGA 2 cut(s) 1508, 1555
XmiI GTMKAC 1 cut(s) 389
XmnI GAANNNNTTC 2 cut(s) 469, 1367
XspI CTAG 3 cut(s) 261, 1509, 1556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.