AT5G57930

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008942
Physical Location & Seq
Forward (+)
23454592 .. 23456526
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57930.2

Sequence Viewer

Length: 1323 bp
ATGTCGATAACGTACTCCGCTATTTCCTTTTCTGGGTTTTCACCAAAAAGTGTACCTTTTGCTATTCATAGCGTAACCAGGAGGCAATTTCTTAATCCCAACACGTTTTATAGATTTGGGTTCTCTCCTTCTCTTCAGGGAAGTAGCATTGAGTTTAGCCTTCAGTTGAATTCAAGAGTTGTGCTTAGTAAAGAGCGTCGATCTCTGCCATTAGTTGTGAGAAATGATCGTCCTCAGAATGAAGACTTACCTAAGCAGTACACAAGGCGAGAGAAGAAACCTTTCCCAGTCCCTATTGTGGACCTGAGACGAGCTGCGAGGGAGAGAGTCAAGAACAACAAAGACAAACCTAAGAGACCTCTACCTCCGCCTAAAAATGGTATGGTTGTGAAGAGCCTTGTGCCTCTTGCTTATAAAGTATACAATGCAAGAATCAGATTGATCAACAATCTCCACCGGCTTATGAAAGTTGTTCGTGTTAATGCTTGTGGGTGGTGCAATGAGATTCATGTTGGACCTTATGGGCATCCATTTAAGTCGTGTAAAGGTCCCAATACTTCCCAAAGGAAAGGTCTTCATGAATGGACTAACTCGGTTATTGAGGACGTTATTGTTCCTCTTGAAGCCTATCACCTTTTTGACCGCCTTGGCAAGCGTATCCGTCATGATGAAAGATTCTCGATTCCCCGAGTTCCTGCTGTAGTTGAGCTCTGCATTCAGGGCGGCGTTGAAATACCCGAATTTCCAGCAAAAAGGAGAAGAAAACCGATTATCCGCATTGGCAAAAGCGAGTTTGTTGATGCAGATGAAACTGAATTGCCTGATCCAGAGCCTCAGCCTCCTCCAGTGCCATTGTTAACTGAGTTACCTGTCTCAGAGATCACTCCCCCATCTAGCGAAGAAGAAACAGTCTCCTTAGCCGAAGAAACATTACAGGCCTGGGAAGAAATGAGAGCAGGAGCCAAAAAGCTGATGAGGATGTACAGGGTTAGGGTCTGTGGGTACTGTCCAGAGGTTCACGTAGGTCCAACGGGACACAAGGCCCAGAACTGTGGTGCATTCAAGCACCAACAGCGGAATGGCCAGCATGGTTGGCAATCTGCAGTACTTGACGACTTGATACCGCCAAGATATGTTTGGCATGTTCCTGATGTGAATGGGCCACCGATGCAGCGAGAGCTACGAAGCTTCTACGGGCAAGCACCTGCTGTTGTGGAGATATGTGCTCAGGCTGGCGCTGTTGTACCTGAGCATTATAGAGCTACAATGAGACTGGAGGTTGGAATTCCTTCGAGTGTGAAAGAAGCTGAGATGGTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

440

Amino Acids

49.94

Weight (kDa)

9.6

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 73 - 270 4e-97 APO RNA-binding
APO_RNA-bind PF05634 305 - 421 9.1e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
AarI CACCTGC 1 cut(s) 1213
Acc36I ACCTGC 1 cut(s) 1213
AccI GTMKAC 1 cut(s) 420
AciI CCGC 7 cut(s) 18, 368, 643, 723, 775, 1075, 1124
AclWI GGATC 1 cut(s) 818
AcoI YGGCCR 1 cut(s) 1081
AcsI RAATTY 3 cut(s) 169, 740, 1284
AcuI CTGAAG 2 cut(s) 119, 146
AfaI GTAC 7 cut(s) 14, 54, 260, 983, 1004, 1107, 1245
AfiI CCNNNNNNNGG 3 cut(s) 33, 298, 377
AflIII ACRYGT 1 cut(s) 102
AgsI TTSAA 5 cut(s) 169, 174, 623, 731, 1063
AjnI CCWGG 2 cut(s) 77, 938
AluBI AGCT 7 cut(s) 314, 709, 970, 1180, 1188, 1262, 1307
AluI AGCT 7 cut(s) 314, 709, 970, 1180, 1188, 1262, 1307
Alw21I GWGCWC 2 cut(s) 711, 1228
Alw26I GTCTC 5 cut(s) 301, 349, 877, 916, 1264
AlwI GGATC 1 cut(s) 818
Ama87I CYCGRG 1 cut(s) 687
AoxI GGCC 4 cut(s) 936, 1041, 1081, 1160
ApeKI GCWGC 2 cut(s) 314, 1171
ApoI RAATTY 3 cut(s) 169, 740, 1284
ArsI GACNNNNNNTTYG 2 cut(s) 556, 588
Asp700I GAANNNNTTC 2 cut(s) 281, 1288
AspLEI GCGC 1 cut(s) 1238
AspS9I GGNCC 6 cut(s) 301, 515, 548, 1025, 1042, 1160
AsuHPI GGTGA 2 cut(s) 33, 623
AvaI CYCGRG 1 cut(s) 687
AvaII GGWCC 4 cut(s) 301, 515, 548, 1025
BalI TGGCCA 1 cut(s) 1083
BanII GRGCYC 1 cut(s) 711
BarI GAAGNNNNNNTAC 2 cut(s) 915, 947
BbsI GAAGAC 2 cut(s) 249, 566
Bbv12I GWGCWC 2 cut(s) 711, 1228
BbvCI CCTCAGC 1 cut(s) 834
BbvI GCAGC 2 cut(s) 301, 1183
BccI CCATC 2 cut(s) 898, 1306
BciT130I CCWGG 2 cut(s) 79, 940
BciVI GTATCC 1 cut(s) 668
BclI TGATCA 1 cut(s) 441
BcoDI GTCTC 5 cut(s) 301, 349, 877, 916, 1264
BfaI CTAG 1 cut(s) 894
BfmI CTRYAG 2 cut(s) 699, 1101
BfoI RGCGCY 1 cut(s) 1239
BfuAI ACCTGC 1 cut(s) 1213
BfuI GTATCC 1 cut(s) 668
BisI GCNGC 3 cut(s) 315, 724, 1172
BlsI GCNGC 3 cut(s) 316, 725, 1173
BmcAI AGTACT 1 cut(s) 1107
Bme1390I CCNGG 2 cut(s) 79, 940
Bme18I GGWCC 4 cut(s) 301, 515, 548, 1025
BmeT110I CYCGRG 1 cut(s) 687
BmgT120I GGNCC 6 cut(s) 301, 515, 548, 1025, 1042, 1160
BmiI GGNNCC 2 cut(s) 550, 961
BmrFI CCNGG 2 cut(s) 79, 940
BmrI ACTGGG 1 cut(s) 281
BmsI GCATC 3 cut(s) 535, 790, 1158
BmuI ACTGGG 1 cut(s) 281
BpiI GAAGAC 2 cut(s) 249, 566
BpmI CTGGAG 2 cut(s) 828, 1295
Bpu10I CCTNAGC 5 cut(s) 252, 834, 916, 1227, 1248
BsaAI YACGTR 1 cut(s) 1021
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 646, 939
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 298, 377
Bse118I RCCGGY 1 cut(s) 456
Bse1I ACTGG 3 cut(s) 287, 845, 1278
Bse3DI GCAATG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 79, 940
BseDI CCNNGG 2 cut(s) 646, 939
BseGI GGATG 2 cut(s) 526, 984
BseLI CCNNNNNNNGG 3 cut(s) 33, 298, 377
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 8 cut(s) 248, 296, 848, 852, 888, 1239, 1241, 1299
BseNI ACTGG 3 cut(s) 287, 845, 1278
BseRI GAGGAG 1 cut(s) 831
BseXI GCAGC 2 cut(s) 301, 1183
BshFI GGCC 4 cut(s) 938, 1043, 1083, 1162
BsiHKAI GWGCWC 2 cut(s) 711, 1228
BsiHKCI CYCGRG 1 cut(s) 687
BsiSI CCGG 1 cut(s) 457
BslFI GGGAC 3 cut(s) 275, 534, 1047
BslI CCNNNNNNNGG 3 cut(s) 33, 298, 377
BsmAI GTCTC 5 cut(s) 301, 349, 877, 916, 1264
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 275, 534, 1047
BsmI GAATGC 2 cut(s) 714, 1058
BsnI GGCC 4 cut(s) 938, 1043, 1083, 1162
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 711, 1228
Bsp1407I TGTACA 1 cut(s) 981
Bsp143I GATC 5 cut(s) 200, 226, 441, 823, 879
BspACI CCGC 7 cut(s) 18, 368, 643, 723, 775, 1075, 1124
BspANI GGCC 4 cut(s) 938, 1043, 1083, 1162
BspCNI CTCAG 8 cut(s) 247, 297, 847, 853, 887, 1240, 1240, 1300
BspHI TCATGA 2 cut(s) 577, 664
BspLI GGNNCC 2 cut(s) 550, 961
BspMAI CTGCAG 1 cut(s) 1105
BspMI ACCTGC 1 cut(s) 1213
BspPI GGATC 1 cut(s) 818
BspQI GCTCTTC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 981
BsrI ACTGG 3 cut(s) 287, 845, 1278
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 2 cut(s) 646, 939
BssMI GATC 5 cut(s) 200, 226, 441, 823, 879
BssNAI GTATAC 1 cut(s) 421
BssT1I CCWWGG 1 cut(s) 646
Bst1107I GTATAC 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 79, 940
Bst4CI ACNGT 3 cut(s) 910, 1007, 1052
Bst6I CTCTTC 2 cut(s) 138, 386
BstAUI TGTACA 1 cut(s) 981
BstBAI YACGTR 1 cut(s) 1021
BstC8I GCNNGC 4 cut(s) 653, 1085, 1200, 1234
BstF5I GGATG 2 cut(s) 526, 984
BstH2I RGCGCY 1 cut(s) 1239
BstHHI GCGC 1 cut(s) 1238
BstKTI GATC 5 cut(s) 203, 229, 444, 826, 882
BstMAI GTCTC 5 cut(s) 301, 349, 877, 916, 1264
BstMBI GATC 5 cut(s) 200, 226, 441, 823, 879
BstMWI GCNNNNNNNGC 5 cut(s) 720, 1072, 1093, 1168, 1177
BstNI CCWGG 2 cut(s) 79, 940
BstNSI RCATGY 1 cut(s) 1145
BstSCI CCNGG 2 cut(s) 77, 938
BstSFI CTRYAG 2 cut(s) 699, 1101
BstV1I GCAGC 2 cut(s) 301, 1183
BstV2I GAAGAC 2 cut(s) 249, 566
BstXI CCANNNNNNTGG 1 cut(s) 1052
BstZ17I GTATAC 1 cut(s) 421
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 4 cut(s) 938, 1043, 1083, 1162
BtsCI GGATG 2 cut(s) 526, 984
BtsIMutI CAGTG 1 cut(s) 852
BveI ACCTGC 1 cut(s) 1213
Cac8I GCNNGC 4 cut(s) 653, 1085, 1200, 1234
CciI TCATGA 2 cut(s) 577, 664
CfoI GCGC 1 cut(s) 1238
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 6 cut(s) 301, 515, 548, 1025, 1042, 1160
CseI GACGC 1 cut(s) 185
Csp6I GTAC 7 cut(s) 13, 53, 259, 982, 1003, 1106, 1244
CviAII CATG 5 cut(s) 509, 578, 665, 1088, 1142
CviQI GTAC 7 cut(s) 13, 53, 259, 982, 1003, 1106, 1244
DpnI GATC 5 cut(s) 202, 228, 443, 825, 881
DpnII GATC 5 cut(s) 200, 226, 441, 823, 879
EaeI YGGCCR 1 cut(s) 1081
Eam1104I CTCTTC 2 cut(s) 138, 386
EarI CTCTTC 2 cut(s) 138, 386
EciI GGCGGA 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 1 cut(s) 646
Eco147I AGGCCT 1 cut(s) 938
Eco24I GRGCYC 1 cut(s) 711
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 4 cut(s) 301, 515, 548, 1025
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 2 cut(s) 119, 146
Eco88I CYCGRG 1 cut(s) 687
EcoICRI GAGCTC 1 cut(s) 709
EcoO109I RGGNCCY 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 169, 1284
EcoRII CCWGG 2 cut(s) 77, 938
EcoT14I CCWWGG 1 cut(s) 646
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 1 cut(s) 646
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 5 cut(s) 512, 581, 668, 1091, 1145
FalI AAGNNNNNCTT 2 cut(s) 40, 72
FaqI GGGAC 3 cut(s) 275, 534, 1047
FatI CATG 5 cut(s) 508, 577, 664, 1087, 1141
FbaI TGATCA 1 cut(s) 441
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 3 cut(s) 315, 724, 1172
FokI GGATG 2 cut(s) 513, 991
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 3 cut(s) 315, 724, 1172
FspBI CTAG 1 cut(s) 894
GlaI GCGC 1 cut(s) 1237
GluI GCNGC 3 cut(s) 315, 724, 1172
GsuI CTGGAG 2 cut(s) 828, 1295
HaeII RGCGCY 1 cut(s) 1239
HaeIII GGCC 4 cut(s) 938, 1043, 1083, 1162
HapII CCGG 1 cut(s) 457
HgaI GACGC 1 cut(s) 185
HhaI GCGC 1 cut(s) 1238
Hin1II CATG 5 cut(s) 512, 581, 668, 1091, 1145
Hin6I GCGC 1 cut(s) 1236
HinP1I GCGC 1 cut(s) 1236
HincII GTYRAC 1 cut(s) 858
HindII GTYRAC 1 cut(s) 858
HindIII AAGCTT 1 cut(s) 1186
HinfI GANTC 5 cut(s) 327, 432, 505, 675, 682
HpaI GTTAAC 1 cut(s) 858
HpaII CCGG 1 cut(s) 457
HphI GGTGA 2 cut(s) 33, 623
Hpy166II GTNNAC 6 cut(s) 53, 261, 301, 421, 858, 1018
Hpy188I TCNGA 3 cut(s) 237, 437, 877
Hpy188III TCNNGA 9 cut(s) 174, 331, 578, 620, 665, 679, 827, 1010, 1148
Hpy8I GTNNAC 6 cut(s) 53, 261, 301, 421, 858, 1018
Hpy99I CGWCG 1 cut(s) 201
HpyAV CCTTC 3 cut(s) 138, 170, 1299
HpyCH4III ACNGT 3 cut(s) 910, 1007, 1052
HpyCH4IV ACGT 4 cut(s) 11, 104, 606, 1020
HpyCH4V TGCA 7 cut(s) 428, 498, 714, 803, 1058, 1103, 1171
HpyF10VI GCNNNNNNNGC 5 cut(s) 720, 1072, 1093, 1168, 1177
HpySE526I ACGT 4 cut(s) 11, 104, 606, 1020
Hsp92II CATG 5 cut(s) 512, 581, 668, 1091, 1145
HspAI GCGC 1 cut(s) 1236
Ksp22I TGATCA 1 cut(s) 441
KspAI GTTAAC 1 cut(s) 858
Kzo9I GATC 5 cut(s) 200, 226, 441, 823, 879
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 1 cut(s) 959
Lsp1109I GCAGC 2 cut(s) 301, 1183
LweI GCATC 3 cut(s) 535, 790, 1158
MaeI CTAG 1 cut(s) 894
MaeII ACGT 4 cut(s) 11, 104, 606, 1020
MaeIII GTNAC 2 cut(s) 73, 864
MalI GATC 5 cut(s) 202, 228, 443, 825, 881
MboI GATC 5 cut(s) 200, 226, 441, 823, 879
MhlI GDGCHC 2 cut(s) 711, 1228
MlsI TGGCCA 1 cut(s) 1083
MluCI AATT 5 cut(s) 86, 169, 740, 815, 1284
MluNI TGGCCA 1 cut(s) 1083
MlyI GAGTC 1 cut(s) 336
MmeI TCCRAC 3 cut(s) 493, 1052, 1261
Mox20I TGGCCA 1 cut(s) 1083
MroXI GAANNNNTTC 2 cut(s) 281, 1288
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 4 cut(s) 93, 480, 534, 857
Msp20I TGGCCA 1 cut(s) 1083
MspA1I CMGCKG 1 cut(s) 1075
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 2 cut(s) 79, 940
Mva1269I GAATGC 2 cut(s) 714, 1058
MvaI CCWGG 2 cut(s) 79, 940
MwoI GCNNNNNNNGC 5 cut(s) 720, 1072, 1093, 1168, 1177
NdeII GATC 5 cut(s) 200, 226, 441, 823, 879
NlaIII CATG 5 cut(s) 512, 581, 668, 1091, 1145
NlaIV GGNNCC 2 cut(s) 550, 961
NspI RCATGY 1 cut(s) 1145
PagI TCATGA 2 cut(s) 577, 664
PaqCI CACCTGC 1 cut(s) 1213
PceI AGGCCT 1 cut(s) 938
PciSI GCTCTTC 1 cut(s) 386
PctI GAATGC 2 cut(s) 714, 1058
PdmI GAANNNNTTC 2 cut(s) 281, 1288
PfeI GAWTC 4 cut(s) 432, 505, 675, 682
PkrI GCNGC 3 cut(s) 316, 725, 1173
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
Ppu21I YACGTR 1 cut(s) 1021
PpuMI RGGWCCY 1 cut(s) 548
PsiI TTATAA 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 711
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 2 cut(s) 77, 938
PspGI CCWGG 2 cut(s) 77, 938
PspN4I GGNNCC 2 cut(s) 550, 961
PspPI GGNCC 6 cut(s) 301, 515, 548, 1025, 1042, 1160
PspPPI RGGWCCY 1 cut(s) 548
PstI CTGCAG 1 cut(s) 1105
RsaI GTAC 7 cut(s) 14, 54, 260, 983, 1004, 1107, 1245
RsaNI GTAC 7 cut(s) 13, 53, 259, 982, 1003, 1106, 1244
SacI GAGCTC 1 cut(s) 711
SapI GCTCTTC 1 cut(s) 386
SaqAI TTAA 4 cut(s) 93, 480, 534, 857
SatI GCNGC 3 cut(s) 315, 724, 1172
Sau3AI GATC 5 cut(s) 200, 226, 441, 823, 879
Sau96I GGNCC 6 cut(s) 301, 515, 548, 1025, 1042, 1160
ScaI AGTACT 1 cut(s) 1107
SchI GAGTC 1 cut(s) 336
ScrFI CCNGG 2 cut(s) 79, 940
SduI GDGCHC 2 cut(s) 711, 1228
SfaNI GCATC 3 cut(s) 535, 790, 1158
SfcI CTRYAG 2 cut(s) 699, 1101
SinI GGWCC 4 cut(s) 301, 515, 548, 1025
Sse9I AATT 5 cut(s) 86, 169, 740, 815, 1284
SseBI AGGCCT 1 cut(s) 938
SsiI CCGC 7 cut(s) 18, 368, 643, 723, 775, 1075, 1124
SspMI CTAG 1 cut(s) 894
SstI GAGCTC 1 cut(s) 711
StuI AGGCCT 1 cut(s) 938
StyD4I CCNGG 2 cut(s) 77, 938
StyI CCWWGG 1 cut(s) 646
TaaI ACNGT 3 cut(s) 910, 1007, 1052
TaiI ACGT 4 cut(s) 14, 107, 609, 1023
TaqI TCGA 4 cut(s) 5, 199, 680, 1292
TasI AATT 5 cut(s) 86, 169, 740, 815, 1284
TatI WGTACW 3 cut(s) 258, 981, 1105
TauI GCSGC 1 cut(s) 726
TfiI GAWTC 4 cut(s) 432, 505, 675, 682
Tru1I TTAA 4 cut(s) 93, 480, 534, 857
Tru9I TTAA 4 cut(s) 93, 480, 534, 857
TscAI CASTG 1 cut(s) 852
TseI GCWGC 2 cut(s) 314, 1171
TspDTI ATGAA 8 cut(s) 56, 255, 479, 497, 566, 594, 684, 822
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 1 cut(s) 852
VpaK11BI GGWCC 4 cut(s) 301, 515, 548, 1025
XapI RAATTY 3 cut(s) 169, 740, 1284
XceI RCATGY 1 cut(s) 1145
XcmI CCANNNNNNNNNTGG 2 cut(s) 1076, 1134
XmiI GTMKAC 1 cut(s) 420
XmnI GAANNNNTTC 2 cut(s) 281, 1288
XspI CTAG 1 cut(s) 894
ZrmI AGTACT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.