Rh6DG357500

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008942
Physical Location & Seq
Forward (+)
55576819 .. 55578617
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG357500.1

Sequence Viewer

Length: 1338 bp
ATGGACTTTCCTTCCAAACTTGGGACCCTGCAGATTTCATCAAAATCAGAATTTCTCAATCCCCACCTCTTCACTTCCCTCCCTTCTTTTCAATTCCAGGTTTCCGCTACTAACCAACTAATCCATTCCTCTGTAAATTTCGCAGTCTCAGTCACTCAATTGTTTCCATTGATTTTGCAGAAGAATATACTCAAGCTTTCATTGAAGCCCAGAAGAGTTTCCCAATTGGTGGTGAGGAGTGAGGATCCTCCTCAAAACTCCGACTTTCCTAAGTACTATTCGAAAAGAGAGAAGAAGCCCTTTCCGGTGCCCATAGTAGATTTGAGGCGTAGGGCAAGGGAGAGGCTTAAGCAGAGCAAGGGCAAGCCCAAAAAGCCAGTCCCACCTCCCAAGAATGGCCTGCTCGTCAAGTCCCTCATACCAGTTGCCTATGATGTGTACAATGCCAGAATCACACTGATCAACAACCTCAAGAAGCTTTTGAAGGTGGTGCCTGTACATGCCTGCAGGTTTTGTAATGAGATTCATGTCGGACCGGTTGGGCATCCGTTTAAGTCATGTAAAGGGCCGGATGCTAGTATCCGAAAGGGGCTTCATGATTGGATAACGAATGCAACTGTGGATGATATTTTCTTGCCTGTTGAGGCTTTCCATTTGTTTGATCGACTTGGGAGGCGAATTCCTCACGTGGAGAGATTCGCAATTCCGAGGATTCCAGCTGTAGTTGAGCTTTGCATTCAGGCTGGGGTTGATATCCCGGAGTGTCCCACTAAGAGGAGGAGAAAGCCGATCATCCGCATTGCGAAAAGTGAGTTTGTTGATGCAGATGAGACTGAGCTGCCAGACCCTGAAGGTCCAAAGGGAACGGTGTTAGCTGAGATACCTGATTCGGAGATTGTGGGGCCGTGTAATGAAGAGGAGAAGATCTTGCTTGCTGAGGAAACGCTACAAGCATGGGAACAGATGAGGAGAGGAGCCAAGAGGCTAATGAAGATGTATTTGGTGAGGGTTTGTGGGTATTGCCCTGAGATTCATGTAGGTCCAAGTGGGCACAAAGCTCAGAACTGTGGTGCCTTCAAGCATCAACAGCGAAATGGGCAGCATGGATGGCAGGCTGCGGTGCTCAATGACTTGATACCACCGAGATATGTGTGGCATGTGCAGGATGTGAATGGACCACCATTGCAACGGGAGCTGAGGAGCTTCTATGGACAAGCCCCTGCGGTGGTTGAAATGTGTGTTCAGGCAGGCGCTGCTGTGCCGGATGAATGCAGACCAACTATGAGGTTGGATGTGGGGATTCCCTCAAATGTTAGAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

445

Amino Acids

50.13

Weight (kDa)

9.41

Isoelectric Point (pI)

50.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 78 - 274 2.4e-90 APO RNA-binding
APO_RNA-bind PF05634 309 - 422 3.6e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 498
AccB1I GGYRCC 3 cut(s) 307, 490, 1070
AccB7I CCANNNNNTGG 1 cut(s) 229
AciI CCGC 4 cut(s) 105, 796, 1118, 1223
AclWI GGATC 2 cut(s) 239, 252
AcsI RAATTY 3 cut(s) 50, 136, 678
AcuI CTGAAG 1 cut(s) 870
AcvI CACGTG 1 cut(s) 688
AfaI GTAC 3 cut(s) 275, 440, 498
AfiI CCNNNNNNNGG 5 cut(s) 21, 229, 395, 774, 1225
AflII CTTAAG 1 cut(s) 347
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 5 cut(s) 92, 205, 484, 1078, 1232
AjnI CCWGG 1 cut(s) 96
AjuI GAANNNNNNNTTGG 2 cut(s) 983, 1015
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 2 cut(s) 151, 824
AlwI GGATC 2 cut(s) 239, 252
AlwNI CAGNNNCTG 2 cut(s) 848, 1253
AoxI GGCC 3 cut(s) 397, 566, 902
ApeKI GCWGC 4 cut(s) 838, 1099, 1115, 1253
ApoI RAATTY 3 cut(s) 50, 136, 678
ArsI GACNNNNNNTTYG 2 cut(s) 363, 395
AsiGI ACCGGT 1 cut(s) 535
Asp700I GAANNNNTTC 1 cut(s) 217
AspLEI GCGC 1 cut(s) 1253
AspS9I GGNCC 7 cut(s) 24, 533, 566, 854, 902, 1040, 1175
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 2 cut(s) 244, 1015
AsuII TTCGAA 1 cut(s) 281
AvaII GGWCC 5 cut(s) 24, 533, 854, 1040, 1175
BaeGI GKGCMC 2 cut(s) 312, 1053
BamHI GGATCC 1 cut(s) 244
BanI GGYRCC 3 cut(s) 307, 490, 1070
BbrPI CACGTG 1 cut(s) 688
Bbv12I GWGCWC 1 cut(s) 1125
BbvCI CCTCAGC 2 cut(s) 936, 1196
BbvI GCAGC 4 cut(s) 825, 1102, 1111, 1240
BccI CCATC 1 cut(s) 1101
BceAI ACGGC 1 cut(s) 889
BciT130I CCWGG 1 cut(s) 98
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 1 cut(s) 459
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 2 cut(s) 151, 824
BfaI CTAG 1 cut(s) 576
BfmI CTRYAG 3 cut(s) 29, 505, 720
BfoI RGCGCY 1 cut(s) 1254
BfrI CTTAAG 1 cut(s) 347
BfuAI ACCTGC 1 cut(s) 498
BfuI GTATCC 1 cut(s) 590
BglII AGATCT 1 cut(s) 924
BisI GCNGC 4 cut(s) 839, 1100, 1116, 1254
BlsI GCNGC 4 cut(s) 840, 1101, 1117, 1255
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 2 cut(s) 98, 758
Bme18I GGWCC 5 cut(s) 24, 533, 854, 1040, 1175
BmgT120I GGNCC 7 cut(s) 24, 533, 566, 854, 902, 1040, 1175
BmiI GGNNCC 8 cut(s) 25, 26, 246, 309, 492, 903, 976, 1072
BmrFI CCNGG 2 cut(s) 98, 758
BmsI GCATC 4 cut(s) 553, 562, 811, 1090
Bpu10I CCTNAGC 2 cut(s) 936, 1196
Bpu14I TTCGAA 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 176, 455
BpuMI CCSGG 1 cut(s) 758
BsaAI YACGTR 1 cut(s) 688
BsaJI CCNNGG 1 cut(s) 707
BsaWI WCCGGW 2 cut(s) 304, 535
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 229, 395, 774, 1225
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 2 cut(s) 377, 422
Bse3DI GCAATG 2 cut(s) 798, 1181
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 1 cut(s) 707
BseGI GGATG 8 cut(s) 544, 577, 628, 792, 1112, 1171, 1270, 1297
BseLI CCNNNNNNNGG 5 cut(s) 21, 229, 395, 774, 1225
BseMI GCAATG 2 cut(s) 798, 1181
BseMII CTCAG 7 cut(s) 162, 825, 867, 927, 1017, 1073, 1187
BseNI ACTGG 2 cut(s) 377, 422
BseRI GAGGAG 8 cut(s) 240, 250, 790, 793, 932, 982, 987, 1213
BseSI GKGCMC 2 cut(s) 312, 1053
BseXI GCAGC 4 cut(s) 825, 1102, 1111, 1240
BseYI CCCAGC 1 cut(s) 743
BsgI GTGCAG 1 cut(s) 1181
BshFI GGCC 3 cut(s) 399, 568, 904
BshNI GGYRCC 3 cut(s) 307, 490, 1070
BshTI ACCGGT 1 cut(s) 535
BsiHKAI GWGCWC 1 cut(s) 1125
BsiSI CCGG 5 cut(s) 305, 536, 569, 758, 1262
BslFI GGGAC 4 cut(s) 37, 365, 397, 750
BslI CCNNNNNNNGG 5 cut(s) 21, 229, 395, 774, 1225
BsmAI GTCTC 2 cut(s) 151, 824
BsmFI GGGAC 4 cut(s) 37, 365, 397, 750
BsmI GAATGC 3 cut(s) 616, 735, 1274
BsnI GGCC 3 cut(s) 399, 568, 904
Bsp119I TTCGAA 1 cut(s) 281
Bsp1286I GDGCHC 3 cut(s) 312, 1053, 1125
Bsp1407I TGTACA 2 cut(s) 438, 496
Bsp143I GATC 5 cut(s) 244, 459, 661, 789, 924
BspACI CCGC 4 cut(s) 105, 796, 1118, 1223
BspANI GGCC 3 cut(s) 399, 568, 904
BspCNI CTCAG 7 cut(s) 161, 826, 868, 928, 1018, 1072, 1188
BspHI TCATGA 1 cut(s) 595
BspLI GGNNCC 8 cut(s) 25, 26, 246, 309, 492, 903, 976, 1072
BspMAI CTGCAG 2 cut(s) 33, 509
BspMI ACCTGC 1 cut(s) 498
BspPI GGATC 2 cut(s) 239, 252
BspT104I TTCGAA 1 cut(s) 281
BspT107I GGYRCC 3 cut(s) 307, 490, 1070
BspTI CTTAAG 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 798, 1181
BsrFI RCCGGY 1 cut(s) 535
BsrGI TGTACA 2 cut(s) 438, 496
BsrI ACTGG 2 cut(s) 377, 422
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 707
BssMI GATC 5 cut(s) 244, 459, 661, 789, 924
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 619, 868, 1067
Bst6I CTCTTC 3 cut(s) 74, 208, 909
BstAFI CTTAAG 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstAUI TGTACA 2 cut(s) 438, 496
BstBAI YACGTR 1 cut(s) 688
BstBI TTCGAA 1 cut(s) 281
BstC8I GCNNGC 6 cut(s) 365, 401, 505, 933, 1113, 1249
BstDEI CTNAG 9 cut(s) 148, 270, 771, 834, 876, 936, 1026, 1059, 1196
BstF5I GGATG 8 cut(s) 544, 577, 628, 792, 1112, 1171, 1270, 1297
BstH2I RGCGCY 1 cut(s) 1254
BstHHI GCGC 1 cut(s) 1253
BstKTI GATC 5 cut(s) 247, 462, 664, 792, 927
BstMAI GTCTC 2 cut(s) 151, 824
BstMBI GATC 5 cut(s) 244, 459, 661, 789, 924
BstMWI GCNNNNNNNGC 6 cut(s) 373, 1087, 1096, 1108, 1192, 1253
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 2 cut(s) 503, 1160
BstSCI CCNGG 2 cut(s) 96, 756
BstSFI CTRYAG 3 cut(s) 29, 505, 720
BstSLI GKGCMC 2 cut(s) 312, 1053
BstV1I GCAGC 4 cut(s) 825, 1102, 1111, 1240
BstX2I RGATCY 2 cut(s) 244, 924
BstYI RGATCY 2 cut(s) 244, 924
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 3 cut(s) 399, 568, 904
BtsCI GGATG 8 cut(s) 544, 577, 628, 792, 1112, 1171, 1270, 1297
BtsIMutI CAGTG 1 cut(s) 455
BveI ACCTGC 1 cut(s) 498
Cac8I GCNNGC 6 cut(s) 365, 401, 505, 933, 1113, 1249
CaiI CAGNNNCTG 2 cut(s) 848, 1253
CciI TCATGA 1 cut(s) 595
CfoI GCGC 1 cut(s) 1253
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 7 cut(s) 24, 533, 566, 854, 902, 1040, 1175
CpoI CGGWCCG 1 cut(s) 533
Csp6I GTAC 3 cut(s) 274, 439, 497
CspAI ACCGGT 1 cut(s) 535
CspI CGGWCCG 1 cut(s) 533
CviAII CATG 8 cut(s) 500, 527, 558, 596, 954, 1034, 1103, 1157
CviQI GTAC 3 cut(s) 274, 439, 497
DdeI CTNAG 9 cut(s) 148, 270, 771, 834, 876, 936, 1026, 1059, 1196
DpnI GATC 5 cut(s) 246, 461, 663, 791, 926
DpnII GATC 5 cut(s) 244, 459, 661, 789, 924
Eam1104I CTCTTC 3 cut(s) 74, 208, 909
EarI CTCTTC 3 cut(s) 74, 208, 909
Eco32I GATATC 1 cut(s) 754
Eco47I GGWCC 5 cut(s) 24, 533, 854, 1040, 1175
Eco57I CTGAAG 1 cut(s) 870
Eco72I CACGTG 1 cut(s) 688
EcoO109I RGGNCCY 1 cut(s) 24
EcoRI GAATTC 1 cut(s) 678
EcoRII CCWGG 1 cut(s) 96
EcoRV GATATC 1 cut(s) 754
FaeI CATG 8 cut(s) 503, 530, 561, 599, 957, 1037, 1106, 1160
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 4 cut(s) 37, 365, 397, 750
FatI CATG 8 cut(s) 499, 526, 557, 595, 953, 1033, 1102, 1156
FbaI TGATCA 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 839, 1100, 1116, 1254
FokI GGATG 8 cut(s) 531, 584, 635, 779, 1119, 1178, 1277, 1304
Fsp4HI GCNGC 4 cut(s) 839, 1100, 1116, 1254
FspBI CTAG 1 cut(s) 576
GlaI GCGC 1 cut(s) 1252
GluI GCNGC 4 cut(s) 839, 1100, 1116, 1254
GsaI CCCAGC 1 cut(s) 747
HaeII RGCGCY 1 cut(s) 1254
HaeIII GGCC 3 cut(s) 399, 568, 904
HapII CCGG 5 cut(s) 305, 536, 569, 758, 1262
HhaI GCGC 1 cut(s) 1253
Hin1II CATG 8 cut(s) 503, 530, 561, 599, 957, 1037, 1106, 1160
Hin6I GCGC 1 cut(s) 1251
HinP1I GCGC 1 cut(s) 1251
HindIII AAGCTT 2 cut(s) 194, 476
HinfI GANTC 7 cut(s) 450, 523, 696, 712, 887, 1030, 1300
HpaII CCGG 5 cut(s) 305, 536, 569, 758, 1262
HphI GGTGA 2 cut(s) 244, 1015
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 7 cut(s) 49, 262, 533, 584, 708, 892, 1062
Hpy188III TCNNGA 2 cut(s) 472, 596
Hpy8I GTNNAC 1 cut(s) 439
HpyAV CCTTC 5 cut(s) 21, 93, 478, 845, 1084
HpyCH4III ACNGT 3 cut(s) 619, 868, 1067
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 9 cut(s) 31, 178, 507, 614, 735, 824, 1162, 1186, 1272
HpyF10VI GCNNNNNNNGC 6 cut(s) 373, 1087, 1096, 1108, 1192, 1253
HpyF3I CTNAG 9 cut(s) 148, 270, 771, 834, 876, 936, 1026, 1059, 1196
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 8 cut(s) 503, 530, 561, 599, 957, 1037, 1106, 1160
HspAI GCGC 1 cut(s) 1251
KflI GGGWCCC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 459
Kzo9I GATC 5 cut(s) 244, 459, 661, 789, 924
LmnI GCTCC 3 cut(s) 974, 1192, 1200
Lsp1109I GCAGC 4 cut(s) 825, 1102, 1111, 1240
LweI GCATC 4 cut(s) 553, 562, 811, 1090
MaeI CTAG 1 cut(s) 576
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 1 cut(s) 151
MalI GATC 5 cut(s) 246, 461, 663, 791, 926
MboI GATC 5 cut(s) 244, 459, 661, 789, 924
MboII GAAGA 7 cut(s) 61, 193, 225, 304, 926, 934, 1003
MfeI CAATTG 2 cut(s) 158, 224
MflI RGATCY 2 cut(s) 244, 924
MhlI GDGCHC 3 cut(s) 312, 1053, 1125
MluCI AATT 7 cut(s) 50, 92, 136, 158, 224, 678, 702
MmeI TCCRAC 3 cut(s) 285, 511, 1269
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 2 cut(s) 348, 552
MspA1I CMGCKG 1 cut(s) 719
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 5 cut(s) 305, 536, 569, 758, 1262
MspR9I CCNGG 2 cut(s) 98, 758
MunI CAATTG 2 cut(s) 158, 224
Mva1269I GAATGC 3 cut(s) 616, 735, 1274
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 6 cut(s) 373, 1087, 1096, 1108, 1192, 1253
NciI CCSGG 1 cut(s) 758
NdeII GATC 5 cut(s) 244, 459, 661, 789, 924
NlaIII CATG 8 cut(s) 503, 530, 561, 599, 957, 1037, 1106, 1160
NlaIV GGNNCC 8 cut(s) 25, 26, 246, 309, 492, 903, 976, 1072
NmuCI GTSAC 1 cut(s) 151
NspI RCATGY 2 cut(s) 503, 1160
NspV TTCGAA 1 cut(s) 281
PagI TCATGA 1 cut(s) 595
PctI GAATGC 3 cut(s) 616, 735, 1274
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 7 cut(s) 450, 523, 696, 712, 887, 1030, 1300
PflMI CCANNNNNTGG 1 cut(s) 229
PfoI TCCNGGA 1 cut(s) 756
PinAI ACCGGT 1 cut(s) 535
PkrI GCNGC 4 cut(s) 840, 1101, 1117, 1255
PmaCI CACGTG 1 cut(s) 688
PmlI CACGTG 1 cut(s) 688
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 743
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 8 cut(s) 25, 26, 246, 309, 492, 903, 976, 1072
PspPI GGNCC 7 cut(s) 24, 533, 566, 854, 902, 1040, 1175
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 2 cut(s) 33, 509
PstNI CAGNNNCTG 2 cut(s) 848, 1253
PsuI RGATCY 2 cut(s) 244, 924
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 3 cut(s) 275, 440, 498
RsaNI GTAC 3 cut(s) 274, 439, 497
Rsr2I CGGWCCG 1 cut(s) 533
RsrII CGGWCCG 1 cut(s) 533
SaqAI TTAA 2 cut(s) 348, 552
SatI GCNGC 4 cut(s) 839, 1100, 1116, 1254
Sau3AI GATC 5 cut(s) 244, 459, 661, 789, 924
Sau96I GGNCC 7 cut(s) 24, 533, 566, 854, 902, 1040, 1175
SbfI CCTGCAGG 1 cut(s) 509
ScaI AGTACT 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 98, 758
SdaI CCTGCAGG 1 cut(s) 509
SduI GDGCHC 3 cut(s) 312, 1053, 1125
SfaNI GCATC 4 cut(s) 553, 562, 811, 1090
SfcI CTRYAG 3 cut(s) 29, 505, 720
SfuI TTCGAA 1 cut(s) 281
SinI GGWCC 5 cut(s) 24, 533, 854, 1040, 1175
SmlI CTYRAG 3 cut(s) 191, 347, 470
SmoI CTYRAG 3 cut(s) 191, 347, 470
Sse8387I CCTGCAGG 1 cut(s) 509
Sse9I AATT 7 cut(s) 50, 92, 136, 158, 224, 678, 702
SsiI CCGC 4 cut(s) 105, 796, 1118, 1223
SspMI CTAG 1 cut(s) 576
StyD4I CCNGG 2 cut(s) 96, 756
TaaI ACNGT 3 cut(s) 619, 868, 1067
TaiI ACGT 1 cut(s) 690
TaqI TCGA 2 cut(s) 281, 664
TasI AATT 7 cut(s) 50, 92, 136, 158, 224, 678, 702
TatI WGTACW 3 cut(s) 273, 438, 496
TfiI GAWTC 7 cut(s) 450, 523, 696, 712, 887, 1030, 1300
Tru1I TTAA 2 cut(s) 348, 552
Tru9I TTAA 2 cut(s) 348, 552
TscAI CASTG 1 cut(s) 462
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 4 cut(s) 838, 1099, 1115, 1253
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 8 cut(s) 27, 189, 515, 584, 927, 1004, 1022, 1281
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 462
Van91I CCANNNNNTGG 1 cut(s) 229
Vha464I CTTAAG 1 cut(s) 347
VpaK11BI GGWCC 5 cut(s) 24, 533, 854, 1040, 1175
XapI RAATTY 3 cut(s) 50, 136, 678
XceI RCATGY 2 cut(s) 503, 1160
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 1 cut(s) 576
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.