MD02G1006800.v1.1

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008942
Physical Location & Seq
Forward (+)
458605 .. 460751
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1006800.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGAATTGCAGCAGCTCTCGTGTCTTCTCCCATGGCGCCGTAGCTTCGTTTCGCGGCAAAGGAATGGACTTTCTCTCTCCCAAATTCGTGACCTTTGGCGCCTCCTTCCCTGCAAAATCAGAATTCCTGAATTCCTACATCCACCCGGCTCTCACTTCCCTCGATTCTTTCCGATTTCAGCAGCAGGGTGGCAATGTACTCCAAGTTTCAGTGAAGTCGAGAAGTTCCAAATCGTATGCATTGGTGGTGAGGAGTGATCATCGCTATCCCCAGAACTCCGACTTTCTGAAGCACTATTCGAAGAAGGAGAAGAAGCCGTTTCCGGTGCCCATTGTGGAGCTGAGGCGGGCAGCAAGAGAGAGGCTGAAGAGCAACAGGGGCAAGCCTAGAACGCCGGTTCCACCTCCCAAAAACGGCTTGCTTGTCAAGACCCTTGTGCCAGTTGCATACGATGTGTACGATGCGAGAATTACATTGATCAACAATTTGAAGAGGCTTTTGAAGGTGGTTCCTGTGCAGGCTTGCAGGTTCTGTAATGAAATTCATGTGGGGCCTGTTGGGCATCCATTCAAATCATGTAAAGGTGCAAATGCTAACATTCGTAAGGGTGTTCATGAGTGGATACCAAATGTGACCGTTGATGACATATTCTTGCCAGTTGAAGCTTTCCACCTCTATGATCGGCTTGGAAGACGCATTCCTCATGAGGAAAGATTCTCAACTCCTAGACTTCCAGCATTAGTTGAGCTCTGCATCCAGGCTGGGGTCGATATCCCAGAATATCCCACTAAAAGGAGAAGAAAGCCAATCATCCGAATTTCAAAGAGTGAATTTGTTGATGCAGATGAAAGTGAGCTACCAGACCTGGACGATGAAGGACCCAAGAGACCCGTTTTGGCTGAAATACTGGATTCGGAGGTAATAGTCCCTTCCGATGAAGAAGAAACAACCTTGCTTGCTGAGGAAACACTACGAGCATGGGAGCAGATGAGGAGAGGAGCCAAGAGATTGATGAAGATGTATCTAGTGAGGGTTTGCGGGTATTGCCCGGAGGTGCATGTTGGTCCTAGCGGCCACAAGGCTCAGAACTGTGGGGCCTTCAAACACCAACAACGAAATGGGCAGCATGGCTGGCAGGCGGCTGTGCTCAATGACTTGATACCACCAAGATATGTGTGGCATGTTCCAGATGTGAATGGACCGCCATTGGAGCGGGAGCTCAGGAACTTTTATGGGCAAGCCCCTGCGATTGTCGAAATGTGCATTCAGGCGGGTGCTGCTGTGCCAGAAGAATACAGACCAACCATGAGGTTGGATGTCGGGATTCCCTCCACTATTAAAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

454

Amino Acids

51.14

Weight (kDa)

9.31

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 84 - 280 6.7e-88 APO RNA-binding
APO_RNA-bind PF05634 319 - 430 6.4e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 516
AccB1I GGYRCC 3 cut(s) 35, 98, 325
AccBSI CCGCTC 1 cut(s) 1213
AccII CGCG 1 cut(s) 54
AciI CCGC 8 cut(s) 54, 346, 1038, 1071, 1139, 1202, 1213, 1271
AcoI YGGCCR 1 cut(s) 1072
AcsI RAATTY 6 cut(s) 83, 122, 130, 540, 816, 830
AcuI CTGAAG 2 cut(s) 308, 386
AcyI GRCGYC 2 cut(s) 36, 99
AfaI GTAC 2 cut(s) 198, 458
AfiI CCNNNNNNNGG 3 cut(s) 413, 763, 792
AgsI TTSAA 6 cut(s) 490, 502, 571, 662, 822, 1102
AjnI CCWGG 2 cut(s) 756, 864
AloI GAACNNNNNNTCC 2 cut(s) 382, 414
AluBI AGCT 8 cut(s) 15, 44, 340, 665, 748, 856, 1219, 1346
AluI AGCT 8 cut(s) 15, 44, 340, 665, 748, 856, 1219, 1346
Alw21I GWGCWC 3 cut(s) 750, 1149, 1221
Alw26I GTCTC 1 cut(s) 880
AlwNI CAGNNNCTG 1 cut(s) 531
AoxI GGCC 3 cut(s) 551, 1072, 1095
ApeKI GCWGC 6 cut(s) 9, 12, 181, 350, 1123, 1277
ApoI RAATTY 6 cut(s) 83, 122, 130, 540, 816, 830
AspLEI GCGC 2 cut(s) 38, 101
AspS9I GGNCC 5 cut(s) 551, 878, 1064, 1095, 1199
AsuC2I CCSGG 2 cut(s) 146, 1049
AsuHPI GGTGA 1 cut(s) 259
AsuII TTCGAA 1 cut(s) 299
AvaII GGWCC 3 cut(s) 878, 1064, 1199
BaeGI GKGCMC 1 cut(s) 330
BanI GGYRCC 3 cut(s) 35, 98, 325
BanII GRGCYC 2 cut(s) 750, 1221
BauI CACGAG 1 cut(s) 18
BbsI GAAGAC 2 cut(s) 16, 697
Bbv12I GWGCWC 3 cut(s) 750, 1149, 1221
BbvCI CCTCAGC 2 cut(s) 341, 960
BbvI GCAGC 6 cut(s) 21, 24, 193, 362, 1135, 1264
BceAI ACGGC 3 cut(s) 23, 301, 430
BciT130I CCWGG 2 cut(s) 758, 866
BciVI GTATCC 1 cut(s) 615
BclI TGATCA 2 cut(s) 256, 477
BcnI CCSGG 2 cut(s) 146, 1049
BcoDI GTCTC 1 cut(s) 880
BfaI CTAG 4 cut(s) 387, 726, 1025, 1068
BfoI RGCGCY 2 cut(s) 39, 102
BfuAI ACCTGC 1 cut(s) 516
BfuI GTATCC 1 cut(s) 615
BglI GCCNNNNNGGC 1 cut(s) 559
BisI GCNGC 9 cut(s) 10, 13, 55, 182, 351, 1072, 1124, 1140, 1278
BlsI GCNGC 9 cut(s) 11, 14, 56, 183, 352, 1073, 1125, 1141, 1279
Bme1390I CCNGG 4 cut(s) 146, 758, 866, 1049
Bme18I GGWCC 3 cut(s) 878, 1064, 1199
BmgT120I GGNCC 5 cut(s) 551, 878, 1064, 1095, 1199
BmiI GGNNCC 9 cut(s) 37, 100, 327, 399, 510, 552, 880, 1000, 1096
BmrFI CCNGG 4 cut(s) 146, 758, 866, 1049
BmsI GCATC 4 cut(s) 451, 571, 762, 829
BpiI GAAGAC 2 cut(s) 16, 697
Bpu10I CCTNAGC 3 cut(s) 341, 960, 1220
Bpu14I TTCGAA 1 cut(s) 299
BpuMI CCSGG 2 cut(s) 146, 1049
BsaHI GRCGYC 2 cut(s) 36, 99
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 3 cut(s) 413, 763, 792
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 3 cut(s) 440, 656, 912
Bse3DI GCAATG 1 cut(s) 199
BseBI CCWGG 2 cut(s) 758, 866
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 5 cut(s) 138, 562, 753, 810, 1321
BseLI CCNNNNNNNGG 3 cut(s) 413, 763, 792
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 4 cut(s) 332, 951, 1097, 1234
BseNI ACTGG 3 cut(s) 440, 656, 912
BseRI GAGGAG 3 cut(s) 265, 1006, 1011
BseSI GKGCMC 1 cut(s) 330
BseXI GCAGC 6 cut(s) 21, 24, 193, 362, 1135, 1264
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 536
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 3 cut(s) 553, 1074, 1097
BshNI GGYRCC 3 cut(s) 35, 98, 325
BsiHKAI GWGCWC 3 cut(s) 750, 1149, 1221
BsiSI CCGG 4 cut(s) 146, 323, 395, 1049
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 3 cut(s) 413, 763, 792
BsmAI GTCTC 1 cut(s) 880
BsmFI GGGAC 1 cut(s) 911
BsmI GAATGC 2 cut(s) 696, 1263
BsnI GGCC 3 cut(s) 553, 1074, 1097
Bso31I GGTCTC 1 cut(s) 880
Bsp119I TTCGAA 1 cut(s) 299
Bsp1286I GDGCHC 4 cut(s) 330, 750, 1149, 1221
Bsp143I GATC 3 cut(s) 256, 477, 679
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 8 cut(s) 54, 346, 1038, 1071, 1139, 1202, 1213, 1271
BspANI GGCC 3 cut(s) 553, 1074, 1097
BspCNI CTCAG 4 cut(s) 333, 952, 1096, 1233
BspFNI CGCG 1 cut(s) 54
BspHI TCATGA 2 cut(s) 613, 703
BspLI GGNNCC 9 cut(s) 37, 100, 327, 399, 510, 552, 880, 1000, 1096
BspMI ACCTGC 1 cut(s) 516
BspQI GCTCTTC 1 cut(s) 362
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 3 cut(s) 35, 98, 325
BspTNI GGTCTC 1 cut(s) 880
BsrBI CCGCTC 1 cut(s) 1213
BsrDI GCAATG 1 cut(s) 199
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 3 cut(s) 440, 656, 912
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 3 cut(s) 256, 477, 679
BssNI GRCGYC 2 cut(s) 36, 99
BssSI CACGAG 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 758, 866
Bst4CI ACNGT 2 cut(s) 637, 1091
Bst6I CTCTTC 2 cut(s) 362, 485
BstACI GRCGYC 2 cut(s) 36, 99
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 9 cut(s) 348, 383, 419, 519, 523, 957, 1133, 1137, 1239
BstDEI CTNAG 4 cut(s) 341, 960, 1083, 1220
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 5 cut(s) 138, 562, 753, 810, 1321
BstFNI CGCG 1 cut(s) 54
BstH2I RGCGCY 2 cut(s) 39, 102
BstHHI GCGC 2 cut(s) 38, 101
BstKTI GATC 3 cut(s) 259, 480, 682
BstMAI GTCTC 1 cut(s) 880
BstMBI GATC 3 cut(s) 256, 477, 679
BstMWI GCNNNNNNNGC 7 cut(s) 378, 391, 559, 1044, 1132, 1210, 1277
BstNI CCWGG 2 cut(s) 758, 866
BstNSI RCATGY 2 cut(s) 1061, 1184
BstSCI CCNGG 4 cut(s) 144, 756, 864, 1047
BstSLI GKGCMC 1 cut(s) 330
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 6 cut(s) 21, 24, 193, 362, 1135, 1264
BstV2I GAAGAC 2 cut(s) 16, 697
BstXI CCANNNNNNTGG 1 cut(s) 1312
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 3 cut(s) 553, 1074, 1097
BtgI CCRYGG 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 245
BtsCI GGATG 5 cut(s) 138, 562, 753, 810, 1321
BtsIMutI CAGTG 1 cut(s) 216
BveI ACCTGC 1 cut(s) 516
Cac8I GCNNGC 9 cut(s) 348, 383, 419, 519, 523, 957, 1133, 1137, 1239
CaiI CAGNNNCTG 1 cut(s) 531
CciI TCATGA 2 cut(s) 613, 703
CfoI GCGC 2 cut(s) 38, 101
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 5 cut(s) 551, 878, 1064, 1095, 1199
CseI GACGC 1 cut(s) 702
Csp6I GTAC 2 cut(s) 197, 457
CviQI GTAC 2 cut(s) 197, 457
DdeI CTNAG 4 cut(s) 341, 960, 1083, 1220
DinI GGCGCC 2 cut(s) 37, 100
DpnI GATC 3 cut(s) 258, 479, 681
DpnII GATC 3 cut(s) 256, 477, 679
EaeI YGGCCR 1 cut(s) 1072
Eam1104I CTCTTC 2 cut(s) 362, 485
EarI CTCTTC 2 cut(s) 362, 485
Ecl136II GAGCTC 2 cut(s) 748, 1219
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 750, 1221
Eco31I GGTCTC 1 cut(s) 880
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 3 cut(s) 878, 1064, 1199
Eco53kI GAGCTC 2 cut(s) 748, 1219
Eco57I CTGAAG 2 cut(s) 308, 386
EcoICRI GAGCTC 2 cut(s) 748, 1219
EcoO109I RGGNCCY 3 cut(s) 551, 878, 1095
EcoRI GAATTC 2 cut(s) 122, 130
EcoRII CCWGG 2 cut(s) 756, 864
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 750, 1221
EgeI GGCGCC 2 cut(s) 37, 100
EheI GGCGCC 2 cut(s) 37, 100
ErhI CCWWGG 1 cut(s) 31
FaqI GGGAC 1 cut(s) 911
FauI CCCGC 4 cut(s) 339, 1031, 1206, 1264
FbaI TGATCA 2 cut(s) 256, 477
Fnu4HI GCNGC 9 cut(s) 10, 13, 55, 182, 351, 1072, 1124, 1140, 1278
FokI GGATG 5 cut(s) 125, 549, 740, 797, 1328
FriOI GRGCYC 2 cut(s) 750, 1221
Fsp4HI GCNGC 9 cut(s) 10, 13, 55, 182, 351, 1072, 1124, 1140, 1278
FspBI CTAG 4 cut(s) 387, 726, 1025, 1068
GlaI GCGC 2 cut(s) 37, 100
GluI GCNGC 9 cut(s) 10, 13, 55, 182, 351, 1072, 1124, 1140, 1278
GsaI CCCAGC 1 cut(s) 765
HaeII RGCGCY 2 cut(s) 39, 102
HaeIII GGCC 3 cut(s) 553, 1074, 1097
HapII CCGG 4 cut(s) 146, 323, 395, 1049
HgaI GACGC 1 cut(s) 702
HhaI GCGC 2 cut(s) 38, 101
Hin1I GRCGYC 2 cut(s) 36, 99
Hin6I GCGC 2 cut(s) 36, 99
HinP1I GCGC 2 cut(s) 36, 99
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 4 cut(s) 164, 714, 911, 1324
HpaII CCGG 4 cut(s) 146, 323, 395, 1049
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 8 cut(s) 121, 173, 280, 288, 815, 916, 934, 1086
Hpy188III TCNNGA 9 cut(s) 88, 127, 219, 427, 614, 704, 1187, 1222, 1321
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 6 cut(s) 115, 298, 496, 869, 939, 1108
HpyCH4III ACNGT 2 cut(s) 637, 1091
HpyF10VI GCNNNNNNNGC 7 cut(s) 378, 391, 559, 1044, 1132, 1210, 1277
HpyF3I CTNAG 4 cut(s) 341, 960, 1083, 1220
Hsp92I GRCGYC 2 cut(s) 36, 99
HspAI GCGC 2 cut(s) 36, 99
KasI GGCGCC 2 cut(s) 35, 98
Ksp22I TGATCA 2 cut(s) 256, 477
Kzo9I GATC 3 cut(s) 256, 477, 679
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 5 cut(s) 337, 982, 998, 1210, 1216
Lsp1109I GCAGC 6 cut(s) 21, 24, 193, 362, 1135, 1264
LweI GCATC 4 cut(s) 451, 571, 762, 829
MaeI CTAG 4 cut(s) 387, 726, 1025, 1068
MaeIII GTNAC 2 cut(s) 88, 631
MalI GATC 3 cut(s) 258, 479, 681
MbiI CCGCTC 1 cut(s) 1213
MboI GATC 3 cut(s) 256, 477, 679
MhlI GDGCHC 4 cut(s) 330, 750, 1149, 1221
MluCI AATT 9 cut(s) 4, 83, 122, 130, 468, 484, 540, 816, 830
Mly113I GGCGCC 2 cut(s) 36, 99
MmeI TCCRAC 2 cut(s) 303, 1293
Mph1103I ATGCAT 1 cut(s) 241
MseI TTAA 1 cut(s) 1338
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 4 cut(s) 146, 323, 395, 1049
MspR9I CCNGG 4 cut(s) 146, 758, 866, 1049
Mva1269I GAATGC 2 cut(s) 696, 1263
MvaI CCWGG 2 cut(s) 758, 866
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 7 cut(s) 378, 391, 559, 1044, 1132, 1210, 1277
NarI GGCGCC 2 cut(s) 36, 99
NciI CCSGG 2 cut(s) 146, 1049
NcoI CCATGG 1 cut(s) 31
NdeII GATC 3 cut(s) 256, 477, 679
NlaIV GGNNCC 9 cut(s) 37, 100, 327, 399, 510, 552, 880, 1000, 1096
NmuCI GTSAC 2 cut(s) 88, 631
NsiI ATGCAT 1 cut(s) 241
NspI RCATGY 2 cut(s) 1061, 1184
NspV TTCGAA 1 cut(s) 299
PagI TCATGA 2 cut(s) 613, 703
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PctI GAATGC 2 cut(s) 696, 1263
PfeI GAWTC 4 cut(s) 164, 714, 911, 1324
PkrI GCNGC 9 cut(s) 11, 14, 56, 183, 352, 1073, 1125, 1141, 1279
PluTI GGCGCC 2 cut(s) 39, 102
PpuMI RGGWCCY 1 cut(s) 878
Psp124BI GAGCTC 2 cut(s) 750, 1221
Psp5II RGGWCCY 1 cut(s) 878
Psp6I CCWGG 2 cut(s) 756, 864
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 2 cut(s) 756, 864
PspN4I GGNNCC 9 cut(s) 37, 100, 327, 399, 510, 552, 880, 1000, 1096
PspPI GGNCC 5 cut(s) 551, 878, 1064, 1095, 1199
PspPPI RGGWCCY 1 cut(s) 878
PstNI CAGNNNCTG 1 cut(s) 531
RsaI GTAC 2 cut(s) 198, 458
RsaNI GTAC 2 cut(s) 197, 457
RseI CAYNNNNRTG 1 cut(s) 675
SacI GAGCTC 2 cut(s) 750, 1221
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 1338
SatI GCNGC 9 cut(s) 10, 13, 55, 182, 351, 1072, 1124, 1140, 1278
Sau3AI GATC 3 cut(s) 256, 477, 679
Sau96I GGNCC 5 cut(s) 551, 878, 1064, 1095, 1199
ScrFI CCNGG 4 cut(s) 146, 758, 866, 1049
SduI GDGCHC 4 cut(s) 330, 750, 1149, 1221
SfaNI GCATC 4 cut(s) 451, 571, 762, 829
SfoI GGCGCC 2 cut(s) 37, 100
SfuI TTCGAA 1 cut(s) 299
SinI GGWCC 3 cut(s) 878, 1064, 1199
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 9 cut(s) 4, 83, 122, 130, 468, 484, 540, 816, 830
SsiI CCGC 8 cut(s) 54, 346, 1038, 1071, 1139, 1202, 1213, 1271
SspDI GGCGCC 2 cut(s) 35, 98
SspMI CTAG 4 cut(s) 387, 726, 1025, 1068
SstI GAGCTC 2 cut(s) 750, 1221
StyD4I CCNGG 4 cut(s) 144, 756, 864, 1047
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 637, 1091
TaqI TCGA 5 cut(s) 162, 218, 299, 768, 1254
TasI AATT 9 cut(s) 4, 83, 122, 130, 468, 484, 540, 816, 830
TatI WGTACW 1 cut(s) 196
TauI GCSGC 3 cut(s) 57, 1074, 1142
TfiI GAWTC 4 cut(s) 164, 714, 911, 1324
Tru1I TTAA 1 cut(s) 1338
Tru9I TTAA 1 cut(s) 1338
TscAI CASTG 1 cut(s) 216
TseFI GTSAC 2 cut(s) 88, 631
TseI GCWGC 6 cut(s) 9, 12, 181, 350, 1123, 1277
Tsp45I GTSAC 2 cut(s) 88, 631
TspDTI ATGAA 8 cut(s) 17, 533, 552, 602, 861, 888, 951, 1028
TspRI CASTG 1 cut(s) 216
VpaK11BI GGWCC 3 cut(s) 878, 1064, 1199
XapI RAATTY 6 cut(s) 83, 122, 130, 540, 816, 830
XceI RCATGY 2 cut(s) 1061, 1184
XcmI CCANNNNNNNNNTGG 2 cut(s) 1115, 1173
XspI CTAG 4 cut(s) 387, 726, 1025, 1068
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.