MD15G1144200.v1.1

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008942
Physical Location & Seq
Forward (+)
10658954 .. 10659883
930 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1144200.v1.1.491

Sequence Viewer

Length: 840 bp
ATGGCGTTCTGTAATGAAATTCATGTGGGGCCTGTTGGGCATCCATTCAAATCATGTAAAGGTGCAAATGCTAACATTCGTAAGGGTGTTCATGAGTGGATACCAAATGTGACCGTTGATGACATATTCTTGCCAGTTGAAGCTTTCCACCTCTATGATCGACTTGGAAGACGTATTCCTCATGAGGAGAGATTCTCAACTCCTAGAGTTCCAGCAATAGTTGAGCTCTGCATCCCGGCTGGGGTTGATGTCCCGGAATATCCCACTACAAGGAGAAGAAAGCCAATCATCCGAATTTTGAAGGGTGACTTTGTTGATGCAGATGAAAGTGAGCTACCAGATCCTGACAGTGAAGCACCCAAGAGACCAGTTTTGACTGAAATATCAGATTCGGAGGTAGTAGCCCCCACTGATGAAGAAGAAACAACCTTGCTTACTGAGGAAACACTACGAGCATGGGAGCAAATGAGGAGAGGAGCCAAGAGGTTGATGAAGATGTATCTGGTGAGGGTTTGCGGGTATTGCCCTGAGGTGCATGTTGGTCCTAGTGGGCACAAGGCTCAGAACTGTGGGGCCTTTAAACACCAACAACGAAATGGGCAGCATGGCTGGCAGACGGCCGTGCTCAATGACTTGATACCACCAAGATATGTGTGGCATGTCCCAGATGTGAATGGATCACCATTGCAGTGGGAGCTCAGGAACTTCTATGGGCAAGCCCCTGCGGTTGTCGAAATGTGCATTCAGGCGGGAGCTGCTGTTCCAGAGGAATACAGACCAACCATGAGGTTGGATGTCGGGATTCCCTCCACTATTAAAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

280

Amino Acids

31.46

Weight (kDa)

5.83

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 3 - 106 8.3e-45 APO RNA-binding
APO_RNA-bind PF05634 147 - 256 2.9e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 516, 725, 749
AclWI GGATC 2 cut(s) 335, 685
AcoI YGGCCR 1 cut(s) 618
AcsI RAATTY 2 cut(s) 18, 294
AfiI CCNNNNNNNGG 2 cut(s) 241, 270
AgsI TTSAA 3 cut(s) 49, 140, 301
AloI GAACNNNNNNTCC 2 cut(s) 744, 776
AluBI AGCT 6 cut(s) 143, 226, 334, 697, 755, 824
AluI AGCT 6 cut(s) 143, 226, 334, 697, 755, 824
Alw21I GWGCWC 3 cut(s) 228, 627, 699
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 2 cut(s) 335, 685
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 3 cut(s) 29, 573, 618
ApeKI GCWGC 2 cut(s) 601, 755
ApoI RAATTY 2 cut(s) 18, 294
AspS9I GGNCC 3 cut(s) 29, 542, 573
AsuC2I CCSGG 2 cut(s) 236, 254
AsuHPI GGTGA 3 cut(s) 317, 517, 672
AvaII GGWCC 1 cut(s) 542
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 555
BanII GRGCYC 2 cut(s) 228, 699
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 3 cut(s) 228, 627, 699
BbvI GCAGC 2 cut(s) 613, 742
BceAI ACGGC 2 cut(s) 605, 633
BciVI GTATCC 1 cut(s) 93
BcnI CCSGG 2 cut(s) 236, 254
BcoDI GTCTC 1 cut(s) 358
BfaI CTAG 2 cut(s) 204, 546
BfuI GTATCC 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 37
BisI GCNGC 2 cut(s) 602, 756
BlsI GCNGC 2 cut(s) 603, 757
Bme1390I CCNGG 2 cut(s) 236, 254
Bme18I GGWCC 1 cut(s) 542
BmgT120I GGNCC 3 cut(s) 29, 542, 573
BmiI GGNNCC 3 cut(s) 30, 478, 574
BmrFI CCNGG 2 cut(s) 236, 254
BmsI GCATC 3 cut(s) 49, 240, 307
BpiI GAAGAC 1 cut(s) 175
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu10I CCTNAGC 1 cut(s) 698
BpuMI CCSGG 2 cut(s) 236, 254
BsaI GGTCTC 1 cut(s) 358
BsaXI ACNNNNNCTCC 2 cut(s) 744, 774
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 270
Bse1I ACTGG 2 cut(s) 134, 368
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 1 cut(s) 683
BseGI GGATG 4 cut(s) 40, 231, 288, 799
BseLI CCNNNNNNNGG 2 cut(s) 241, 270
BseMI GCAATG 1 cut(s) 683
BseMII CTCAG 4 cut(s) 429, 519, 575, 712
BseNI ACTGG 2 cut(s) 134, 368
BseRI GAGGAG 3 cut(s) 200, 484, 489
BseSI GKGCMC 1 cut(s) 555
BseX3I CGGCCG 1 cut(s) 618
BseXI GCAGC 2 cut(s) 613, 742
BseYI CCCAGC 1 cut(s) 239
Bsh1285I CGRYCG 1 cut(s) 621
BshFI GGCC 3 cut(s) 31, 575, 620
BsiEI CGRYCG 1 cut(s) 621
BsiHKAI GWGCWC 3 cut(s) 228, 627, 699
BsiSI CCGG 2 cut(s) 236, 254
BslFI GGGAC 2 cut(s) 236, 647
BslI CCNNNNNNNGG 2 cut(s) 241, 270
BsmAI GTCTC 1 cut(s) 358
BsmFI GGGAC 2 cut(s) 236, 647
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 3 cut(s) 31, 575, 620
Bso31I GGTCTC 1 cut(s) 358
Bsp1286I GDGCHC 4 cut(s) 228, 555, 627, 699
Bsp143I GATC 3 cut(s) 157, 340, 677
BspACI CCGC 3 cut(s) 516, 725, 749
BspANI GGCC 3 cut(s) 31, 575, 620
BspCNI CTCAG 4 cut(s) 430, 520, 574, 711
BspHI TCATGA 2 cut(s) 91, 181
BspLI GGNNCC 3 cut(s) 30, 478, 574
BspPI GGATC 2 cut(s) 335, 685
BspTNI GGTCTC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 683
BsrI ACTGG 2 cut(s) 134, 368
BssMI GATC 3 cut(s) 157, 340, 677
Bst4CI ACNGT 3 cut(s) 115, 350, 569
BstC8I GCNNGC 2 cut(s) 611, 717
BstDEI CTNAG 4 cut(s) 438, 528, 561, 698
BstF5I GGATG 4 cut(s) 40, 231, 288, 799
BstKTI GATC 3 cut(s) 160, 343, 680
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 3 cut(s) 157, 340, 677
BstMCI CGRYCG 1 cut(s) 621
BstMWI GCNNNNNNNGC 5 cut(s) 37, 522, 610, 694, 755
BstNSI RCATGY 2 cut(s) 539, 662
BstSCI CCNGG 2 cut(s) 234, 252
BstSLI GKGCMC 1 cut(s) 555
BstV1I GCAGC 2 cut(s) 613, 742
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 340
BstXI CCANNNNNNTGG 2 cut(s) 690, 790
BstYI RGATCY 1 cut(s) 340
BstZI CGGCCG 1 cut(s) 618
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 93
BsuRI GGCC 3 cut(s) 31, 575, 620
BtsCI GGATG 4 cut(s) 40, 231, 288, 799
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 3 cut(s) 355, 408, 695
Cac8I GCNNGC 2 cut(s) 611, 717
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 2 cut(s) 91, 181
Cfr13I GGNCC 3 cut(s) 29, 542, 573
CviAII CATG 9 cut(s) 23, 54, 92, 182, 456, 536, 605, 659, 784
DdeI CTNAG 4 cut(s) 438, 528, 561, 698
DpnI GATC 3 cut(s) 159, 342, 679
DpnII GATC 3 cut(s) 157, 340, 677
DraI TTTAAA 1 cut(s) 580
EaeI YGGCCR 1 cut(s) 618
EagI CGGCCG 1 cut(s) 618
Ecl136II GAGCTC 2 cut(s) 226, 697
EclXI CGGCCG 1 cut(s) 618
Eco24I GRGCYC 2 cut(s) 228, 699
Eco31I GGTCTC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 542
Eco52I CGGCCG 1 cut(s) 618
Eco53kI GAGCTC 2 cut(s) 226, 697
Eco81I CCTNAGG 1 cut(s) 528
EcoICRI GAGCTC 2 cut(s) 226, 697
EcoO109I RGGNCCY 2 cut(s) 29, 573
EcoT38I GRGCYC 2 cut(s) 228, 699
FaeI CATG 9 cut(s) 26, 57, 95, 185, 459, 539, 608, 662, 787
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 2 cut(s) 236, 647
FatI CATG 9 cut(s) 22, 53, 91, 181, 455, 535, 604, 658, 783
FauI CCCGC 2 cut(s) 509, 742
Fnu4HI GCNGC 2 cut(s) 602, 756
FokI GGATG 4 cut(s) 27, 218, 275, 806
FriOI GRGCYC 2 cut(s) 228, 699
Fsp4HI GCNGC 2 cut(s) 602, 756
FspBI CTAG 2 cut(s) 204, 546
GluI GCNGC 2 cut(s) 602, 756
GsaI CCCAGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 31, 575, 620
HapII CCGG 2 cut(s) 236, 254
Hin1II CATG 9 cut(s) 26, 57, 95, 185, 459, 539, 608, 662, 787
HindIII AAGCTT 1 cut(s) 141
HinfI GANTC 3 cut(s) 192, 389, 802
HpaII CCGG 2 cut(s) 236, 254
HphI GGTGA 3 cut(s) 317, 517, 672
Hpy188I TCNGA 4 cut(s) 293, 388, 394, 564
Hpy188III TCNNGA 6 cut(s) 92, 182, 344, 700, 764, 799
HpyAV CCTTC 1 cut(s) 295
HpyCH4III ACNGT 3 cut(s) 115, 350, 569
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 6 cut(s) 65, 231, 320, 535, 688, 741
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 522, 610, 694, 755
HpyF3I CTNAG 4 cut(s) 438, 528, 561, 698
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 9 cut(s) 26, 57, 95, 185, 459, 539, 608, 662, 787
Kzo9I GATC 3 cut(s) 157, 340, 677
LmnI GCTCC 4 cut(s) 460, 476, 694, 752
Lsp1109I GCAGC 2 cut(s) 613, 742
LweI GCATC 3 cut(s) 49, 240, 307
MaeI CTAG 2 cut(s) 204, 546
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 2 cut(s) 109, 305
MalI GATC 3 cut(s) 159, 342, 679
MboI GATC 3 cut(s) 157, 340, 677
MboII GAAGA 5 cut(s) 180, 288, 428, 431, 505
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 4 cut(s) 228, 555, 627, 699
MluCI AATT 2 cut(s) 18, 294
MmeI TCCRAC 1 cut(s) 771
MseI TTAA 2 cut(s) 579, 816
MslI CAYNNNNRTG 2 cut(s) 153, 688
MspI CCGG 2 cut(s) 236, 254
MspR9I CCNGG 2 cut(s) 236, 254
Mva1269I GAATGC 1 cut(s) 741
MwoI GCNNNNNNNGC 5 cut(s) 37, 522, 610, 694, 755
NciI CCSGG 2 cut(s) 236, 254
NdeII GATC 3 cut(s) 157, 340, 677
NlaIII CATG 9 cut(s) 26, 57, 95, 185, 459, 539, 608, 662, 787
NlaIV GGNNCC 3 cut(s) 30, 478, 574
NmuCI GTSAC 2 cut(s) 109, 305
NspI RCATGY 2 cut(s) 539, 662
PagI TCATGA 2 cut(s) 91, 181
PctI GAATGC 1 cut(s) 741
PfeI GAWTC 3 cut(s) 192, 389, 802
PfoI TCCNGGA 1 cut(s) 252
PkrI GCNGC 2 cut(s) 603, 757
Psp124BI GAGCTC 2 cut(s) 228, 699
PspFI CCCAGC 1 cut(s) 239
PspN4I GGNNCC 3 cut(s) 30, 478, 574
PspPI GGNCC 3 cut(s) 29, 542, 573
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 340
RseI CAYNNNNRTG 2 cut(s) 153, 688
SacI GAGCTC 2 cut(s) 228, 699
SaqAI TTAA 2 cut(s) 579, 816
SatI GCNGC 2 cut(s) 602, 756
Sau3AI GATC 3 cut(s) 157, 340, 677
Sau96I GGNCC 3 cut(s) 29, 542, 573
ScrFI CCNGG 2 cut(s) 236, 254
SduI GDGCHC 4 cut(s) 228, 555, 627, 699
SfaNI GCATC 3 cut(s) 49, 240, 307
SinI GGWCC 1 cut(s) 542
SmiMI CAYNNNNRTG 2 cut(s) 153, 688
Sse9I AATT 2 cut(s) 18, 294
SsiI CCGC 3 cut(s) 516, 725, 749
SspMI CTAG 2 cut(s) 204, 546
SstI GAGCTC 2 cut(s) 228, 699
StyD4I CCNGG 2 cut(s) 234, 252
TaaI ACNGT 3 cut(s) 115, 350, 569
TaiI ACGT 1 cut(s) 175
TaqI TCGA 2 cut(s) 160, 732
TasI AATT 2 cut(s) 18, 294
TfiI GAWTC 3 cut(s) 192, 389, 802
Tru1I TTAA 2 cut(s) 579, 816
Tru9I TTAA 2 cut(s) 579, 816
TscAI CASTG 3 cut(s) 355, 415, 695
TseFI GTSAC 2 cut(s) 109, 305
TseI GCWGC 2 cut(s) 601, 755
Tsp45I GTSAC 2 cut(s) 109, 305
TspDTI ATGAA 6 cut(s) 11, 30, 80, 339, 429, 506
TspRI CASTG 3 cut(s) 355, 415, 695
VpaK11BI GGWCC 1 cut(s) 542
XapI RAATTY 2 cut(s) 18, 294
XceI RCATGY 2 cut(s) 539, 662
XcmI CCANNNNNNNNNTGG 2 cut(s) 593, 651
XspI CTAG 2 cut(s) 204, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.