Prupe.7G263600_v2.0.a1

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0008942
Physical Location & Seq
Reverse (-)
21834025 .. 21836419
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G263600.1

Sequence Viewer

Length: 1350 bp
ATGAATAGCAGCTCTCATTATTTCTCACATGGCACTGTAGCTTTGTTCTGTGGTAAAGGAATGGACTTTCGTCCCAAAGTTGGGACCTTGCAGGCTTCCTTCAATGGAAAATCAGAATTTCTGAATCTCCACGTTTACCCTGCTCTTACTTCCATTGATTCTTTTCGATTTCAGGAGCATGGTTGCAATGTACTGAAGCTTTCATTGAAGTCAAGAACTTCCCAATCGCAAGCATTGGTGGTGAGGAGCGATCATCCTCAAAATTCTGACTTTCCGAAATACTATTCGAAAAAGGAGAAGAAGCCCTTTCCAGTCCCCATTGTGGACTTGAGGCGGGCTGCCAGGGAAAGGCTTAAGAGCAGGAAGGGCAAGCCTAAAACACCAGTTCCTCCTCCCAAGAATGGCTTGCTTGTCAAGACCCTTATCCCAGTTGCCTATGACGTCTTCAATGCAAGAATTACATTGATCAATAATTTGAAGAAGCTGTTAAAGGTGGTGCCTGTGCAAGCTTGCAGGTTCTGTAATGAAATTCATGTGGGGCCTGTTGGGCATCCGTTCAAATCATGTAAAGGTGCAAATGCTAACATTCGTAAGGGTGTTCATGAGTGGATAACAAATGCGACCGTTGATGACGTATTCTTACCAGTTGAAGCTTTCCATCTCTATGATCGCCTTGGAAGACGCATTCCTCATGAGGAGAGATTCTCAATTCCTAGAATTCCAGCAATAGTTGAGCTTTGCATCCAAGCTGGGGTTGATGTCGCAGAATATCCCACTAAAAGGAGAAGAAAGCCAATCATCCGCATTTCAAAAAGTGAATTTGTTGATGCAGATGAAAGTGAGCTACCAGACACTGACACTGAAGGACCCAAGAGACCCGTCTTGACTGAAATACCCGATTCAGAGATAGTAGCCCCATCAGATGAAGAAGAAACAACCTTGCTTGCTGAGGAAACACTACGAGCATGGGACCAGATGAGGAGAGGAGCCAAGAGGCTAATGAAGATGTATCTGGTGAGGGTTTGTGGGTATTGCCCTGAGGTTCATGTGGGTCCCAGTGGGCACAAGGCTCAGAACTGTGGAGCCTTCAAACACCAACAACGAAATGGGCAACATGGTTGGCAGGCGGCAGTGCTCAATGACTTGATACCACCAAGATATGTGTGGCATGTTCCAGATGTAAATGGACCACCAATGGAAAGGGAGCTCAGGAACTTCTATGGGCAAGCCCCTGCTGTTGTTGAAATGTGCATTCAGGCGGGTGCTGCTGTGCCAGAAGAATACAGACCAACCATGAGGTTGGATGTTGGAATTCCCTCAACCATTAAAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

450

Amino Acids

50.58

Weight (kDa)

9.25

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 504
AccB1I GGYRCC 1 cut(s) 496
AciI CCGC 4 cut(s) 334, 802, 1127, 1259
AcsI RAATTY 6 cut(s) 116, 262, 528, 717, 818, 1311
AcuI CTGAAG 2 cut(s) 215, 882
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 1 cut(s) 192
AfiI CCNNNNNNNGG 7 cut(s) 80, 81, 322, 348, 401, 751, 780
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 9 cut(s) 103, 208, 448, 478, 559, 650, 810, 1090, 1244
AjnI CCWGG 1 cut(s) 341
Alw21I GWGCWC 2 cut(s) 1137, 1209
Alw26I GTCTC 1 cut(s) 868
AlwNI CAGNNNCTG 2 cut(s) 519, 854
AoxI GGCC 1 cut(s) 539
ApeKI GCWGC 3 cut(s) 9, 338, 1265
ApoI RAATTY 6 cut(s) 116, 262, 528, 717, 818, 1311
AspS9I GGNCC 6 cut(s) 84, 539, 866, 970, 1052, 1187
AsuHPI GGTGA 2 cut(s) 253, 1027
AsuII TTCGAA 1 cut(s) 287
AvaII GGWCC 5 cut(s) 84, 866, 970, 1052, 1187
AxyI CCTNAGG 1 cut(s) 1038
BaeGI GKGCMC 1 cut(s) 1065
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 1209
BbsI GAAGAC 2 cut(s) 436, 685
Bbv12I GWGCWC 2 cut(s) 1137, 1209
BbvCI CCTCAGC 1 cut(s) 948
BbvI GCAGC 3 cut(s) 21, 325, 1252
BccI CCATC 2 cut(s) 666, 925
BciT130I CCWGG 1 cut(s) 343
BclI TGATCA 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 868
BfaI CTAG 1 cut(s) 714
BfmI CTRYAG 1 cut(s) 36
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 504
BglI GCCNNNNNGGC 1 cut(s) 547
BisI GCNGC 4 cut(s) 10, 339, 1128, 1266
BlsI GCNGC 4 cut(s) 11, 340, 1129, 1267
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 5 cut(s) 84, 866, 970, 1052, 1187
BmgT120I GGNCC 6 cut(s) 84, 539, 866, 970, 1052, 1187
BmiI GGNNCC 9 cut(s) 85, 498, 540, 868, 971, 988, 1053, 1054, 1084
BmrFI CCNGG 1 cut(s) 343
BmrI ACTGGG 2 cut(s) 422, 1050
BmsI GCATC 3 cut(s) 559, 750, 817
BmuI ACTGGG 2 cut(s) 422, 1050
BoxI GACNNNNGTC 1 cut(s) 69
BpiI GAAGAC 2 cut(s) 436, 685
BplI GAGNNNNNCTC 2 cut(s) 689, 721
Bpu10I CCTNAGC 2 cut(s) 948, 1208
Bpu14I TTCGAA 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 349
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 868
BsaJI CCNNGG 2 cut(s) 342, 673
Bsc4I CCNNNNNNNGG 7 cut(s) 80, 81, 322, 348, 401, 751, 780
Bse1I ACTGG 5 cut(s) 311, 383, 428, 644, 1056
Bse21I CCTNAGG 1 cut(s) 1038
Bse3DI GCAATG 1 cut(s) 193
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 2 cut(s) 342, 673
BseGI GGATG 5 cut(s) 253, 550, 741, 798, 1309
BseLI CCNNNNNNNGG 7 cut(s) 80, 81, 322, 348, 401, 751, 780
BseMI GCAATG 1 cut(s) 193
BseMII CTCAG 4 cut(s) 939, 1029, 1085, 1222
BseNI ACTGG 5 cut(s) 311, 383, 428, 644, 1056
BseRI GAGGAG 5 cut(s) 259, 381, 710, 994, 999
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 3 cut(s) 21, 325, 1252
BseYI CCCAGC 1 cut(s) 749
Bsh1285I CGRYCG 1 cut(s) 624
BshFI GGCC 1 cut(s) 541
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 624
BsiHKAI GWGCWC 2 cut(s) 1137, 1209
BslFI GGGAC 5 cut(s) 57, 97, 299, 983, 1038
BslI CCNNNNNNNGG 7 cut(s) 80, 81, 322, 348, 401, 751, 780
BsmAI GTCTC 1 cut(s) 868
BsmFI GGGAC 5 cut(s) 57, 97, 299, 983, 1038
BsmI GAATGC 2 cut(s) 684, 1251
BsnI GGCC 1 cut(s) 541
Bso31I GGTCTC 1 cut(s) 868
Bsp119I TTCGAA 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 1065, 1137, 1209
Bsp143I GATC 3 cut(s) 250, 465, 667
BspACI CCGC 4 cut(s) 334, 802, 1127, 1259
BspANI GGCC 1 cut(s) 541
BspCNI CTCAG 4 cut(s) 940, 1030, 1084, 1221
BspHI TCATGA 2 cut(s) 601, 691
BspLI GGNNCC 9 cut(s) 85, 498, 540, 868, 971, 988, 1053, 1054, 1084
BspMI ACCTGC 1 cut(s) 504
BspT104I TTCGAA 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 496
BspTI CTTAAG 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 868
BsrDI GCAATG 1 cut(s) 193
BsrI ACTGG 5 cut(s) 311, 383, 428, 644, 1056
BssECI CCNNGG 2 cut(s) 342, 673
BssMI GATC 3 cut(s) 250, 465, 667
BssNI GRCGYC 1 cut(s) 441
BssT1I CCWWGG 1 cut(s) 673
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 3 cut(s) 37, 625, 1079
BstACI GRCGYC 1 cut(s) 441
BstAFI CTTAAG 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 287
BstDEI CTNAG 4 cut(s) 948, 1038, 1071, 1208
BstF5I GGATG 5 cut(s) 253, 550, 741, 798, 1309
BstKTI GATC 3 cut(s) 253, 468, 670
BstMAI GTCTC 1 cut(s) 868
BstMBI GATC 3 cut(s) 250, 465, 667
BstMCI CGRYCG 1 cut(s) 624
BstMWI GCNNNNNNNGC 3 cut(s) 366, 547, 1265
BstNI CCWGG 1 cut(s) 343
BstNSI RCATGY 1 cut(s) 1172
BstPAI GACNNNNGTC 1 cut(s) 69
BstSCI CCNGG 1 cut(s) 341
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 1065
BstV1I GCAGC 3 cut(s) 21, 325, 1252
BstV2I GAAGAC 2 cut(s) 436, 685
BstXI CCANNNNNNTGG 1 cut(s) 1300
Bsu36I CCTNAGG 1 cut(s) 1038
BsuRI GGCC 1 cut(s) 541
BtsCI GGATG 5 cut(s) 253, 550, 741, 798, 1309
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 5 cut(s) 33, 852, 858, 1063, 1137
BveI ACCTGC 1 cut(s) 504
CaiI CAGNNNCTG 2 cut(s) 519, 854
CciI TCATGA 2 cut(s) 601, 691
Cfr13I GGNCC 6 cut(s) 84, 539, 866, 970, 1052, 1187
CseI GACGC 1 cut(s) 690
Csp6I GTAC 1 cut(s) 191
CviQI GTAC 1 cut(s) 191
DdeI CTNAG 4 cut(s) 948, 1038, 1071, 1208
DpnI GATC 3 cut(s) 252, 467, 669
DpnII GATC 3 cut(s) 250, 465, 667
Ecl136II GAGCTC 1 cut(s) 1207
Eco130I CCWWGG 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 1209
Eco31I GGTCTC 1 cut(s) 868
Eco47I GGWCC 5 cut(s) 84, 866, 970, 1052, 1187
Eco53kI GAGCTC 1 cut(s) 1207
Eco57I CTGAAG 2 cut(s) 215, 882
Eco81I CCTNAGG 1 cut(s) 1038
EcoICRI GAGCTC 1 cut(s) 1207
EcoO109I RGGNCCY 4 cut(s) 84, 539, 866, 1052
EcoRI GAATTC 2 cut(s) 717, 1311
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 1209
ErhI CCWWGG 1 cut(s) 673
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 389, 421
FaqI GGGAC 5 cut(s) 57, 97, 299, 983, 1038
FauI CCCGC 2 cut(s) 327, 1252
FbaI TGATCA 1 cut(s) 465
Fnu4HI GCNGC 4 cut(s) 10, 339, 1128, 1266
FokI GGATG 5 cut(s) 240, 537, 728, 785, 1316
FriOI GRGCYC 1 cut(s) 1209
Fsp4HI GCNGC 4 cut(s) 10, 339, 1128, 1266
FspBI CTAG 1 cut(s) 714
GluI GCNGC 4 cut(s) 10, 339, 1128, 1266
GsaI CCCAGC 1 cut(s) 753
HaeIII GGCC 1 cut(s) 541
HgaI GACGC 1 cut(s) 690
Hin1I GRCGYC 1 cut(s) 441
HindIII AAGCTT 3 cut(s) 197, 507, 651
HinfI GANTC 4 cut(s) 124, 158, 702, 899
HphI GGTGA 2 cut(s) 253, 1027
Hpy166II GTNNAC 2 cut(s) 136, 325
Hpy188I TCNGA 7 cut(s) 115, 123, 268, 276, 904, 922, 1074
Hpy188III TCNNGA 8 cut(s) 173, 213, 415, 602, 692, 883, 1175, 1210
Hpy8I GTNNAC 2 cut(s) 136, 325
HpyAV CCTTC 4 cut(s) 109, 358, 857, 1096
HpyCH4III ACNGT 3 cut(s) 37, 625, 1079
HpyCH4IV ACGT 3 cut(s) 132, 441, 633
HpyCH4V TGCA 9 cut(s) 91, 186, 452, 505, 513, 575, 741, 830, 1251
HpyF10VI GCNNNNNNNGC 3 cut(s) 366, 547, 1265
HpyF3I CTNAG 4 cut(s) 948, 1038, 1071, 1208
HpySE526I ACGT 3 cut(s) 132, 441, 633
Hsp92I GRCGYC 1 cut(s) 441
KflI GGGWCCC 1 cut(s) 1052
Ksp22I TGATCA 1 cut(s) 465
Kzo9I GATC 3 cut(s) 250, 465, 667
LmnI GCTCC 5 cut(s) 175, 246, 986, 1082, 1204
Lsp1109I GCAGC 3 cut(s) 21, 325, 1252
LweI GCATC 3 cut(s) 559, 750, 817
MaeI CTAG 1 cut(s) 714
MaeII ACGT 3 cut(s) 132, 441, 633
MalI GATC 3 cut(s) 252, 467, 669
MboI GATC 3 cut(s) 250, 465, 667
MboII GAAGA 9 cut(s) 310, 436, 490, 690, 798, 938, 941, 1015, 1289
MhlI GDGCHC 3 cut(s) 1065, 1137, 1209
MluCI AATT 9 cut(s) 116, 262, 456, 472, 528, 708, 717, 818, 1311
MmeI TCCRAC 2 cut(s) 1281, 1288
MseI TTAA 3 cut(s) 354, 488, 1326
MslI CAYNNNNRTG 1 cut(s) 663
MspCI CTTAAG 1 cut(s) 353
MspR9I CCNGG 1 cut(s) 343
Mva1269I GAATGC 2 cut(s) 684, 1251
MvaI CCWGG 1 cut(s) 343
MwoI GCNNNNNNNGC 3 cut(s) 366, 547, 1265
NdeII GATC 3 cut(s) 250, 465, 667
NlaIV GGNNCC 9 cut(s) 85, 498, 540, 868, 971, 988, 1053, 1054, 1084
NspI RCATGY 1 cut(s) 1172
NspV TTCGAA 1 cut(s) 287
PagI TCATGA 2 cut(s) 601, 691
PctI GAATGC 2 cut(s) 684, 1251
PfeI GAWTC 4 cut(s) 124, 158, 702, 899
PkrI GCNGC 4 cut(s) 11, 340, 1129, 1267
PpuMI RGGWCCY 3 cut(s) 84, 866, 1052
PshAI GACNNNNGTC 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 1209
Psp5II RGGWCCY 3 cut(s) 84, 866, 1052
Psp6I CCWGG 1 cut(s) 341
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 1 cut(s) 341
PspN4I GGNNCC 9 cut(s) 85, 498, 540, 868, 971, 988, 1053, 1054, 1084
PspPI GGNCC 6 cut(s) 84, 539, 866, 970, 1052, 1187
PspPPI RGGWCCY 3 cut(s) 84, 866, 1052
PstNI CAGNNNCTG 2 cut(s) 519, 854
RsaI GTAC 1 cut(s) 192
RsaNI GTAC 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 663
SacI GAGCTC 1 cut(s) 1209
SaqAI TTAA 3 cut(s) 354, 488, 1326
SatI GCNGC 4 cut(s) 10, 339, 1128, 1266
Sau3AI GATC 3 cut(s) 250, 465, 667
Sau96I GGNCC 6 cut(s) 84, 539, 866, 970, 1052, 1187
ScrFI CCNGG 1 cut(s) 343
SduI GDGCHC 3 cut(s) 1065, 1137, 1209
SfaNI GCATC 3 cut(s) 559, 750, 817
SfcI CTRYAG 1 cut(s) 36
SfuI TTCGAA 1 cut(s) 287
SinI GGWCC 5 cut(s) 84, 866, 970, 1052, 1187
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 2 cut(s) 328, 353
SmoI CTYRAG 2 cut(s) 328, 353
Sse9I AATT 9 cut(s) 116, 262, 456, 472, 528, 708, 717, 818, 1311
SsiI CCGC 4 cut(s) 334, 802, 1127, 1259
SspMI CTAG 1 cut(s) 714
SstI GAGCTC 1 cut(s) 1209
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 1 cut(s) 673
TaaI ACNGT 3 cut(s) 37, 625, 1079
TaiI ACGT 3 cut(s) 135, 444, 636
TaqI TCGA 2 cut(s) 166, 287
TasI AATT 9 cut(s) 116, 262, 456, 472, 528, 708, 717, 818, 1311
TatI WGTACW 1 cut(s) 190
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 4 cut(s) 124, 158, 702, 899
Tru1I TTAA 3 cut(s) 354, 488, 1326
Tru9I TTAA 3 cut(s) 354, 488, 1326
TscAI CASTG 5 cut(s) 40, 859, 865, 1063, 1137
TseI GCWGC 3 cut(s) 9, 338, 1265
TspDTI ATGAA 9 cut(s) 17, 192, 521, 540, 590, 849, 939, 1016, 1034
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 5 cut(s) 40, 859, 865, 1063, 1137
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 5 cut(s) 84, 866, 970, 1052, 1187
XapI RAATTY 6 cut(s) 116, 262, 528, 717, 818, 1311
XceI RCATGY 1 cut(s) 1172
XcmI CCANNNNNNNNNTGG 2 cut(s) 1103, 1161
XspI CTAG 1 cut(s) 714
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.