Rh6BG363500

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0008942
Physical Location & Seq
Forward (+)
59343552 .. 59345378
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG363500.1

Sequence Viewer

Length: 1257 bp
ATGGACTTTCCTTCCAAACTTGGGACCCTGCAGATTTCATCAAAATCAGAATTTCTCAATCCCCACCTCTTCACTTCCCTCCCTTCTTTTCAATTTCAGAAGAATATACTCAAGCTTTCATTGAAGCCCAGAAGAGTTTCCCAATTGGTGGTGAGGAGTGAGGATCCTCCTCAAAACTCCGACTTTCCCAAGTACTATTCGAAAAGAGAGAAGAAGCCCTTTCCGGTGCCCATAGTAGATTTGAGGCGTAGGGCAAGGGAGAGGCTTAAGCAGAGCAAGGGCAAGCCCAAAAAGCCAGTCCCACCTCCCAAGAATGGCCTGCTCGTCAAGTCCCTCATACCAGTTGCCTATGATGTGTACAATGCCAGAATCACACTGATCAACAACCTCAAGAAGCTTTTGAAGGTGGTGCCTGTACATGCCTGCAGGTTTTGTAATGAGATTCATGTCGGACCGGTTGGGCATCCGTTTAAGTCATGTAAAGGGCCGGATGCTAGTATCCGAAAGGGGCTTCATGATTGGATAACGAATGCGACTGTGGATGATATTTTCTTGCCTGTTGAGGCTTTCCACTTGTTTGATCGACTTGGGAGGCGAATTCCTCATGTGGAGAGATTCGCAATTCCGAGGATTCCAGCAGTAGTTGAGCTTTGCATTCAGGCTGGGGTTGATATCCCGGAGTATCCCACTAAGAGGAGGAGAAAGCCGATCATCCGCATTGCGAAAAGTGAGTTTGTTGATGCAGATGAGAGTGAGCTGCCAGACCCTGAAGGTCCTAAGGGAACGGTGTTAGCTGAGATACCTGATTCGGAGATTGTGGGGCCCTGTAATGAAGAGGAGAAGATCTTGCTTGCTGAGGAAACGCTACAAGCATGGGAACAGATGAGGAGAGGAGCCAAGAGGCTAATGAAGATGTATTTAGTGAGGGTTTGTGGGTATTGCCCTGAGATTCATGTAGGTCCAAGTGGGCATAAAGCTCAGAACTGTGGTGCCTTCAAGCATCAACAGAGAAATGGGCAGCATGGATGGCAGGCTGCGGTGCTCAATGATTTGATACCACCGAGATATGTGTGGCATGTGCCGGATGTGAATGGACCACCATTGCAACGGGAGCTGAGGAGCTTCTATGGACAAGCCCCCGCAGTGGTTGAAATGTGTGTTCAGGCAGGTGCTGCTGTGCCGGATGAATGCAGACCAACTATGAGGTTGGATGTGGGGATTCCCTCAAATGTTAGAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

418

Amino Acids

47.23

Weight (kDa)

9.44

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APO_RNA-bind PF05634 51 - 247 7.8e-92 APO RNA-binding
APO_RNA-bind PF05634 282 - 395 7.1e-25 APO RNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1157
Acc36I ACCTGC 2 cut(s) 417, 1157
AccB1I GGYRCC 3 cut(s) 226, 409, 989
AccB7I CCANNNNNTGG 1 cut(s) 148
AciI CCGC 3 cut(s) 715, 1037, 1140
AclWI GGATC 2 cut(s) 158, 171
AcsI RAATTY 2 cut(s) 50, 597
AcuI CTGAAG 1 cut(s) 789
AfaI GTAC 3 cut(s) 194, 359, 417
AfiI CCNNNNNNNGG 5 cut(s) 21, 148, 314, 693, 1144
AflII CTTAAG 1 cut(s) 266
AgeI ACCGGT 1 cut(s) 454
AgsI TTSAA 5 cut(s) 92, 124, 403, 997, 1151
AluBI AGCT 9 cut(s) 115, 397, 649, 757, 794, 977, 1114, 1122, 1241
AluI AGCT 9 cut(s) 115, 397, 649, 757, 794, 977, 1114, 1122, 1241
Alw21I GWGCWC 1 cut(s) 1044
AlwI GGATC 2 cut(s) 158, 171
AlwNI CAGNNNCTG 2 cut(s) 767, 1172
AoxI GGCC 3 cut(s) 316, 485, 821
ApaI GGGCCC 1 cut(s) 825
ApeKI GCWGC 4 cut(s) 757, 1018, 1034, 1172
ApoI RAATTY 2 cut(s) 50, 597
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AsiGI ACCGGT 1 cut(s) 454
Asp700I GAANNNNTTC 1 cut(s) 136
AspS9I GGNCC 8 cut(s) 24, 452, 485, 773, 821, 822, 959, 1094
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 1 cut(s) 163
AsuII TTCGAA 1 cut(s) 200
AvaII GGWCC 5 cut(s) 24, 452, 773, 959, 1094
AxyI CCTNAGG 1 cut(s) 777
BaeGI GKGCMC 2 cut(s) 231, 825
BamHI GGATCC 1 cut(s) 163
BanI GGYRCC 3 cut(s) 226, 409, 989
BanII GRGCYC 1 cut(s) 825
Bbv12I GWGCWC 1 cut(s) 1044
BbvCI CCTCAGC 2 cut(s) 855, 1115
BbvI GCAGC 4 cut(s) 744, 1021, 1030, 1159
BccI CCATC 1 cut(s) 1020
BciVI GTATCC 2 cut(s) 509, 693
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 1 cut(s) 677
BfaI CTAG 1 cut(s) 495
BfmI CTRYAG 2 cut(s) 29, 424
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 417, 1157
BfuI GTATCC 2 cut(s) 509, 693
BglII AGATCT 1 cut(s) 843
BisI GCNGC 4 cut(s) 758, 1019, 1035, 1173
BlsI GCNGC 4 cut(s) 759, 1020, 1036, 1174
BmcAI AGTACT 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 5 cut(s) 24, 452, 773, 959, 1094
BmgT120I GGNCC 8 cut(s) 24, 452, 485, 773, 821, 822, 959, 1094
BmiI GGNNCC 9 cut(s) 25, 26, 165, 228, 411, 822, 823, 895, 991
BmrFI CCNGG 1 cut(s) 677
BmsI GCATC 4 cut(s) 472, 481, 730, 1009
Bpu10I CCTNAGC 2 cut(s) 855, 1115
Bpu14I TTCGAA 1 cut(s) 200
BpuEI CTTGAG 2 cut(s) 95, 374
BpuMI CCSGG 1 cut(s) 677
BsaJI CCNNGG 1 cut(s) 626
BsaWI WCCGGW 2 cut(s) 223, 454
Bsc4I CCNNNNNNNGG 5 cut(s) 21, 148, 314, 693, 1144
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 2 cut(s) 296, 341
Bse21I CCTNAGG 1 cut(s) 777
Bse3DI GCAATG 2 cut(s) 717, 1100
BseDI CCNNGG 1 cut(s) 626
BseGI GGATG 8 cut(s) 463, 496, 547, 711, 1031, 1090, 1189, 1216
BseLI CCNNNNNNNGG 5 cut(s) 21, 148, 314, 693, 1144
BseMI GCAATG 2 cut(s) 717, 1100
BseMII CTCAG 5 cut(s) 786, 846, 936, 992, 1106
BseNI ACTGG 2 cut(s) 296, 341
BseRI GAGGAG 8 cut(s) 159, 169, 709, 712, 851, 901, 906, 1132
BseSI GKGCMC 2 cut(s) 231, 825
BseXI GCAGC 4 cut(s) 744, 1021, 1030, 1159
BseYI CCCAGC 1 cut(s) 662
BshFI GGCC 3 cut(s) 318, 487, 823
BshNI GGYRCC 3 cut(s) 226, 409, 989
BshTI ACCGGT 1 cut(s) 454
BsiHKAI GWGCWC 1 cut(s) 1044
BsiSI CCGG 6 cut(s) 224, 455, 488, 677, 1082, 1181
BslFI GGGAC 3 cut(s) 37, 284, 316
BslI CCNNNNNNNGG 5 cut(s) 21, 148, 314, 693, 1144
BsmFI GGGAC 3 cut(s) 37, 284, 316
BsmI GAATGC 3 cut(s) 535, 654, 1193
BsnI GGCC 3 cut(s) 318, 487, 823
Bsp119I TTCGAA 1 cut(s) 200
Bsp120I GGGCCC 1 cut(s) 821
Bsp1286I GDGCHC 3 cut(s) 231, 825, 1044
Bsp1407I TGTACA 2 cut(s) 357, 415
Bsp143I GATC 5 cut(s) 163, 378, 580, 708, 843
BspACI CCGC 3 cut(s) 715, 1037, 1140
BspANI GGCC 3 cut(s) 318, 487, 823
BspCNI CTCAG 5 cut(s) 787, 847, 937, 991, 1107
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 9 cut(s) 25, 26, 165, 228, 411, 822, 823, 895, 991
BspMAI CTGCAG 2 cut(s) 33, 428
BspMI ACCTGC 2 cut(s) 417, 1157
BspPI GGATC 2 cut(s) 158, 171
BspT104I TTCGAA 1 cut(s) 200
BspT107I GGYRCC 3 cut(s) 226, 409, 989
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 2 cut(s) 717, 1100
BsrFI RCCGGY 1 cut(s) 454
BsrGI TGTACA 2 cut(s) 357, 415
BsrI ACTGG 2 cut(s) 296, 341
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 626
BssMI GATC 5 cut(s) 163, 378, 580, 708, 843
Bst4CI ACNGT 3 cut(s) 538, 787, 986
Bst6I CTCTTC 3 cut(s) 74, 127, 828
BstAFI CTTAAG 1 cut(s) 266
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstAUI TGTACA 2 cut(s) 357, 415
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 5 cut(s) 284, 320, 424, 852, 1032
BstDEI CTNAG 7 cut(s) 690, 777, 795, 855, 945, 978, 1115
BstF5I GGATG 8 cut(s) 463, 496, 547, 711, 1031, 1090, 1189, 1216
BstKTI GATC 5 cut(s) 166, 381, 583, 711, 846
BstMBI GATC 5 cut(s) 163, 378, 580, 708, 843
BstMWI GCNNNNNNNGC 4 cut(s) 292, 1027, 1111, 1172
BstNSI RCATGY 2 cut(s) 422, 1079
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 2 cut(s) 29, 424
BstSLI GKGCMC 2 cut(s) 231, 825
BstV1I GCAGC 4 cut(s) 744, 1021, 1030, 1159
BstX2I RGATCY 2 cut(s) 163, 843
BstYI RGATCY 2 cut(s) 163, 843
Bsu36I CCTNAGG 1 cut(s) 777
BsuI GTATCC 2 cut(s) 509, 693
BsuRI GGCC 3 cut(s) 318, 487, 823
BtsCI GGATG 8 cut(s) 463, 496, 547, 711, 1031, 1090, 1189, 1216
BtsI GCAGTG 1 cut(s) 1149
BtsIMutI CAGTG 2 cut(s) 374, 1149
BveI ACCTGC 2 cut(s) 417, 1157
Cac8I GCNNGC 5 cut(s) 284, 320, 424, 852, 1032
CaiI CAGNNNCTG 2 cut(s) 767, 1172
CciI TCATGA 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 8 cut(s) 24, 452, 485, 773, 821, 822, 959, 1094
CpoI CGGWCCG 1 cut(s) 452
Csp6I GTAC 3 cut(s) 193, 358, 416
CspAI ACCGGT 1 cut(s) 454
CspI CGGWCCG 1 cut(s) 452
CviAII CATG 9 cut(s) 419, 446, 477, 515, 605, 873, 953, 1022, 1076
CviQI GTAC 3 cut(s) 193, 358, 416
DdeI CTNAG 7 cut(s) 690, 777, 795, 855, 945, 978, 1115
DpnI GATC 5 cut(s) 165, 380, 582, 710, 845
DpnII GATC 5 cut(s) 163, 378, 580, 708, 843
Eam1104I CTCTTC 3 cut(s) 74, 127, 828
EarI CTCTTC 3 cut(s) 74, 127, 828
Eco24I GRGCYC 1 cut(s) 825
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 5 cut(s) 24, 452, 773, 959, 1094
Eco57I CTGAAG 1 cut(s) 789
Eco81I CCTNAGG 1 cut(s) 777
EcoO109I RGGNCCY 4 cut(s) 24, 773, 821, 822
EcoRI GAATTC 1 cut(s) 597
EcoRV GATATC 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 825
FaeI CATG 9 cut(s) 422, 449, 480, 518, 608, 876, 956, 1025, 1079
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 3 cut(s) 37, 284, 316
FatI CATG 9 cut(s) 418, 445, 476, 514, 604, 872, 952, 1021, 1075
FauI CCCGC 1 cut(s) 1147
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 4 cut(s) 758, 1019, 1035, 1173
FokI GGATG 8 cut(s) 450, 503, 554, 698, 1038, 1097, 1196, 1223
FriOI GRGCYC 1 cut(s) 825
Fsp4HI GCNGC 4 cut(s) 758, 1019, 1035, 1173
FspBI CTAG 1 cut(s) 495
GluI GCNGC 4 cut(s) 758, 1019, 1035, 1173
GsaI CCCAGC 1 cut(s) 666
HaeIII GGCC 3 cut(s) 318, 487, 823
HapII CCGG 6 cut(s) 224, 455, 488, 677, 1082, 1181
Hin1II CATG 9 cut(s) 422, 449, 480, 518, 608, 876, 956, 1025, 1079
HindIII AAGCTT 2 cut(s) 113, 395
HinfI GANTC 7 cut(s) 369, 442, 615, 631, 806, 949, 1219
HpaII CCGG 6 cut(s) 224, 455, 488, 677, 1082, 1181
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 8 cut(s) 49, 99, 181, 452, 503, 627, 811, 981
Hpy188III TCNNGA 2 cut(s) 391, 515
Hpy8I GTNNAC 1 cut(s) 358
HpyAV CCTTC 5 cut(s) 21, 93, 397, 764, 1003
HpyCH4III ACNGT 3 cut(s) 538, 787, 986
HpyCH4V TGCA 6 cut(s) 31, 426, 654, 743, 1105, 1191
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 1027, 1111, 1172
HpyF3I CTNAG 7 cut(s) 690, 777, 795, 855, 945, 978, 1115
Hsp92II CATG 9 cut(s) 422, 449, 480, 518, 608, 876, 956, 1025, 1079
KflI GGGWCCC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 5 cut(s) 163, 378, 580, 708, 843
LmnI GCTCC 3 cut(s) 893, 1111, 1119
Lsp1109I GCAGC 4 cut(s) 744, 1021, 1030, 1159
LweI GCATC 4 cut(s) 472, 481, 730, 1009
MaeI CTAG 1 cut(s) 495
MalI GATC 5 cut(s) 165, 380, 582, 710, 845
MboI GATC 5 cut(s) 163, 378, 580, 708, 843
MboII GAAGA 7 cut(s) 61, 112, 144, 223, 845, 853, 922
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 163, 843
MhlI GDGCHC 3 cut(s) 231, 825, 1044
MluCI AATT 5 cut(s) 50, 92, 143, 597, 621
MmeI TCCRAC 3 cut(s) 204, 430, 1188
MroXI GAANNNNTTC 1 cut(s) 136
MseI TTAA 2 cut(s) 267, 471
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 6 cut(s) 224, 455, 488, 677, 1082, 1181
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 3 cut(s) 535, 654, 1193
MwoI GCNNNNNNNGC 4 cut(s) 292, 1027, 1111, 1172
NciI CCSGG 1 cut(s) 677
NdeII GATC 5 cut(s) 163, 378, 580, 708, 843
NlaIII CATG 9 cut(s) 422, 449, 480, 518, 608, 876, 956, 1025, 1079
NlaIV GGNNCC 9 cut(s) 25, 26, 165, 228, 411, 822, 823, 895, 991
NspI RCATGY 2 cut(s) 422, 1079
NspV TTCGAA 1 cut(s) 200
PagI TCATGA 1 cut(s) 514
PaqCI CACCTGC 1 cut(s) 1157
PctI GAATGC 3 cut(s) 535, 654, 1193
PdmI GAANNNNTTC 1 cut(s) 136
PfeI GAWTC 7 cut(s) 369, 442, 615, 631, 806, 949, 1219
PflMI CCANNNNNTGG 1 cut(s) 148
PfoI TCCNGGA 1 cut(s) 675
PinAI ACCGGT 1 cut(s) 454
PkrI GCNGC 4 cut(s) 759, 1020, 1036, 1174
PpuMI RGGWCCY 2 cut(s) 24, 773
Psp5II RGGWCCY 2 cut(s) 24, 773
PspFI CCCAGC 1 cut(s) 662
PspN4I GGNNCC 9 cut(s) 25, 26, 165, 228, 411, 822, 823, 895, 991
PspOMI GGGCCC 1 cut(s) 821
PspPI GGNCC 8 cut(s) 24, 452, 485, 773, 821, 822, 959, 1094
PspPPI RGGWCCY 2 cut(s) 24, 773
PstI CTGCAG 2 cut(s) 33, 428
PstNI CAGNNNCTG 2 cut(s) 767, 1172
PsuI RGATCY 2 cut(s) 163, 843
RsaI GTAC 3 cut(s) 194, 359, 417
RsaNI GTAC 3 cut(s) 193, 358, 416
Rsr2I CGGWCCG 1 cut(s) 452
RsrII CGGWCCG 1 cut(s) 452
SaqAI TTAA 2 cut(s) 267, 471
SatI GCNGC 4 cut(s) 758, 1019, 1035, 1173
Sau3AI GATC 5 cut(s) 163, 378, 580, 708, 843
Sau96I GGNCC 8 cut(s) 24, 452, 485, 773, 821, 822, 959, 1094
SbfI CCTGCAGG 1 cut(s) 428
ScaI AGTACT 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 677
SdaI CCTGCAGG 1 cut(s) 428
SduI GDGCHC 3 cut(s) 231, 825, 1044
SfaNI GCATC 4 cut(s) 472, 481, 730, 1009
SfcI CTRYAG 2 cut(s) 29, 424
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 5 cut(s) 24, 452, 773, 959, 1094
SmlI CTYRAG 3 cut(s) 110, 266, 389
SmoI CTYRAG 3 cut(s) 110, 266, 389
Sse8387I CCTGCAGG 1 cut(s) 428
Sse9I AATT 5 cut(s) 50, 92, 143, 597, 621
SsiI CCGC 3 cut(s) 715, 1037, 1140
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 675
TaaI ACNGT 3 cut(s) 538, 787, 986
TaqI TCGA 2 cut(s) 200, 583
TasI AATT 5 cut(s) 50, 92, 143, 597, 621
TatI WGTACW 3 cut(s) 192, 357, 415
TfiI GAWTC 7 cut(s) 369, 442, 615, 631, 806, 949, 1219
Tru1I TTAA 2 cut(s) 267, 471
Tru9I TTAA 2 cut(s) 267, 471
TscAI CASTG 2 cut(s) 381, 1149
TseI GCWGC 4 cut(s) 757, 1018, 1034, 1172
TspDTI ATGAA 8 cut(s) 27, 108, 434, 503, 846, 923, 941, 1200
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 2 cut(s) 381, 1149
Van91I CCANNNNNTGG 1 cut(s) 148
Vha464I CTTAAG 1 cut(s) 266
VpaK11BI GGWCC 5 cut(s) 24, 452, 773, 959, 1094
XapI RAATTY 2 cut(s) 50, 597
XceI RCATGY 2 cut(s) 422, 1079
XmnI GAANNNNTTC 1 cut(s) 136
XspI CTAG 1 cut(s) 495
ZrmI AGTACT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.