pycom15g13580

APO protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008942
Physical Location & Seq
Forward (+)
9234301 .. 9236297
1997 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g13580.3

Sequence Viewer

Length: 1398 bp
ATGCCGTTTAACATTGGAATGGATGAGACGGCATCGCATAACCGAAGCAGACGGACTGATTATCTTTGCTGCGAAAGCCAGAGCACTTTGCTCTCAGAAATGGATTGCAGCAGCTCTCATAGCTTCTCCCATGGCGCCGTAGCTTCATTCCACGGAAAAGGAACAAACTTTCTTCCTCCAAAAGTCGGCACTTTTCGGGCTTCCTTCAATGCAAAATCAGAATTCCTGAATTCCCACCTGTACCCTTCTCTCACTTCCCTCGATTCTTTCCGATTTCAGTTACTAATCCCATTCCTGTTCAGAATTGATCAACTGGGGTTTTCAGTGAAGTCGAGAACTTCGAAATCATATGCATTGGTGGTGAGGAGTGACCACCGTCATCCACAGAACTCCGACTTTCCACGGTACTATTCGAAGAAGGAGAAGAAGCCCTTTCCGGTGCCGATTGTGGAGCTGAGGCGGGCGGCGAGGGAGAGGCTTAAGAGCAACAAGGGAAAGGCTAGGACCCCAGTTCCACCTCCCAAAAATGGTTTGCTCGTCAAGATGGGTGCCAGTTCCGTGTTCTGTAATGAAATTCATGTGGGGCCTGTTGGGCATCCATTCAAATCATGTAAAGGTGCAAATGCTAACATTCGTAAGGGTGTTCATGAGTGGATACCAAATGTGACCGTTGATGACATATTCTTGCCGGTTGAAGCTTTCCACCACTATGATCGGCTTGGAAGACATATTCCTCATGATGAGAGATTCTCAACTCCTAGAGTTCCAGCAATAGTTGAGCTCTGCATCCAGGCTGGGGTTGATGTCCCGGAATATCCCACTACGAGGAGAAGAATGCCAATCATCCGAACTTCAAAGAGTGAATTTGTTGATGCGGATGAAAGTGAGCTACCAGATCCTGACAGTGGACCCAAGAGACCAGTTTTGACTGAAATATCGGATTTGGAGGTAGTAGCCCCCTCTGATGAAGAAGAAACAACCTTGCTTGCTGAGGAAACACTACGAGCATGGGAGAAAATGAGGAGAGGAGCCAAGAGGCTGATGAAGATGTATCTGGTGAGGGTTTGCGGGTATTGCCCTGAGGTGCATGTCGGTCCTAGTAGTAGTGGGCACAAGGCTCAGAACTGTGGGGCCTTCAAACACCAACAACGAAATGGGCAGCATGGCTGGCAGGCGGCCATGCTCAATAACTTGATACCACCAAGATATGTGTGGCATGTTCCAGATGTGAATGGACCACCATTGGAGCGGGAGCTCAGGAACTTCTATGGGCAAGCCCCTGCGGTTGTCGAAATGTGCATTCAGGCAGGAGCTGCTGTTTCAGAGGAATACAGACCAACCATGAGGTTGGATGTCGGGATTCCCTCCACTATTAAAGAAGCTGAAATGGTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
APO_RNA-bind

Protein Analysis

466

Amino Acids

52.33

Weight (kDa)

8.97

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 134, 439, 548
AccBSI CCGCTC 1 cut(s) 1249
AciI CCGC 7 cut(s) 460, 464, 875, 1068, 1175, 1249, 1283
AclWI GGATC 1 cut(s) 890
AcoI YGGCCR 1 cut(s) 1176
AcsI RAATTY 4 cut(s) 221, 229, 573, 863
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 2 cut(s) 242, 407
AfiI CCNNNNNNNGG 5 cut(s) 185, 527, 796, 825, 905
AflII CTTAAG 1 cut(s) 479
AgsI TTSAA 5 cut(s) 208, 604, 695, 855, 1138
AjnI CCWGG 1 cut(s) 789
Alw21I GWGCWC 3 cut(s) 86, 783, 1257
Alw26I GTCTC 2 cut(s) 20, 910
AlwI GGATC 1 cut(s) 890
AlwNI CAGNNNCTG 2 cut(s) 899, 1313
AoxI GGCC 3 cut(s) 584, 1131, 1176
ApeKI GCWGC 5 cut(s) 69, 108, 111, 1159, 1313
ApoI RAATTY 4 cut(s) 221, 229, 573, 863
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 6 cut(s) 504, 584, 908, 1094, 1131, 1235
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 2 cut(s) 373, 1069
AsuII TTCGAA 2 cut(s) 341, 413
AvaII GGWCC 4 cut(s) 504, 908, 1094, 1235
AxyI CCTNAGG 1 cut(s) 1080
BaeGI GKGCMC 1 cut(s) 1113
BanI GGYRCC 3 cut(s) 134, 439, 548
BanII GRGCYC 2 cut(s) 783, 1257
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 3 cut(s) 86, 783, 1257
BbvCI CCTCAGC 2 cut(s) 455, 990
BbvI GCAGC 5 cut(s) 56, 120, 123, 1171, 1300
BccI CCATC 1 cut(s) 538
BceAI ACGGC 2 cut(s) 45, 122
BciT130I CCWGG 1 cut(s) 791
BciVI GTATCC 1 cut(s) 648
BclI TGATCA 1 cut(s) 307
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 2 cut(s) 20, 910
BfaI CTAG 3 cut(s) 501, 759, 1098
BfoI RGCGCY 1 cut(s) 138
BfrI CTTAAG 1 cut(s) 479
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 592
BisI GCNGC 7 cut(s) 70, 109, 112, 465, 1160, 1176, 1314
BlsI GCNGC 7 cut(s) 71, 110, 113, 466, 1161, 1177, 1315
Bme1390I CCNGG 2 cut(s) 791, 809
Bme18I GGWCC 4 cut(s) 504, 908, 1094, 1235
BmgT120I GGNCC 6 cut(s) 504, 584, 908, 1094, 1131, 1235
BmiI GGNNCC 8 cut(s) 136, 441, 506, 550, 585, 910, 1030, 1132
BmrFI CCNGG 2 cut(s) 791, 809
BmrI ACTGGG 2 cut(s) 323, 503
BmsI GCATC 4 cut(s) 41, 604, 795, 862
BmuI ACTGGG 2 cut(s) 323, 503
BoxI GACNNNNGTC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 730
BplI GAGNNNNNCTC 2 cut(s) 734, 766
Bpu10I CCTNAGC 3 cut(s) 455, 990, 1256
Bpu14I TTCGAA 2 cut(s) 341, 413
BpuMI CCSGG 1 cut(s) 809
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 910
BsaJI CCNNGG 3 cut(s) 130, 151, 401
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 1302, 1332
Bsc4I CCNNNNNNNGG 5 cut(s) 185, 527, 796, 825, 905
Bse118I RCCGGY 1 cut(s) 688
Bse1I ACTGG 4 cut(s) 318, 509, 552, 920
Bse21I CCTNAGG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 791
BseDI CCNNGG 3 cut(s) 130, 151, 401
BseGI GGATG 7 cut(s) 28, 379, 595, 786, 843, 883, 1357
BseLI CCNNNNNNNGG 5 cut(s) 185, 527, 796, 825, 905
BseMII CTCAG 6 cut(s) 108, 446, 981, 1071, 1133, 1270
BseNI ACTGG 4 cut(s) 318, 509, 552, 920
BseRI GAGGAG 4 cut(s) 379, 841, 1036, 1041
BseSI GKGCMC 1 cut(s) 1113
BseXI GCAGC 5 cut(s) 56, 120, 123, 1171, 1300
BseYI CCCAGC 1 cut(s) 794
BshFI GGCC 3 cut(s) 586, 1133, 1178
BshNI GGYRCC 3 cut(s) 134, 439, 548
BsiHKAI GWGCWC 3 cut(s) 86, 783, 1257
BsiSI CCGG 3 cut(s) 437, 689, 809
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 5 cut(s) 185, 527, 796, 825, 905
BsmAI GTCTC 2 cut(s) 20, 910
BsmBI CGTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 2 cut(s) 840, 1299
BsnI GGCC 3 cut(s) 586, 1133, 1178
Bso31I GGTCTC 1 cut(s) 910
Bsp119I TTCGAA 2 cut(s) 341, 413
Bsp1286I GDGCHC 4 cut(s) 86, 783, 1113, 1257
Bsp143I GATC 3 cut(s) 307, 712, 895
Bsp19I CCATGG 1 cut(s) 130
BspACI CCGC 7 cut(s) 460, 464, 875, 1068, 1175, 1249, 1283
BspANI GGCC 3 cut(s) 586, 1133, 1178
BspCNI CTCAG 6 cut(s) 107, 447, 982, 1072, 1132, 1269
BspHI TCATGA 2 cut(s) 646, 736
BspLI GGNNCC 8 cut(s) 136, 441, 506, 550, 585, 910, 1030, 1132
BspPI GGATC 1 cut(s) 890
BspT104I TTCGAA 2 cut(s) 341, 413
BspT107I GGYRCC 3 cut(s) 134, 439, 548
BspTI CTTAAG 1 cut(s) 479
BspTNI GGTCTC 1 cut(s) 910
BsrBI CCGCTC 1 cut(s) 1249
BsrFI RCCGGY 1 cut(s) 688
BsrI ACTGG 4 cut(s) 318, 509, 552, 920
BssAI RCCGGY 1 cut(s) 688
BssECI CCNNGG 3 cut(s) 130, 151, 401
BssMI GATC 3 cut(s) 307, 712, 895
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 791
Bst4CI ACNGT 5 cut(s) 377, 405, 670, 905, 1127
BstACI GRCGYC 1 cut(s) 135
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstBI TTCGAA 2 cut(s) 341, 413
BstC8I GCNNGC 5 cut(s) 462, 987, 1169, 1173, 1275
BstDEI CTNAG 6 cut(s) 94, 455, 990, 1080, 1119, 1256
BstDSI CCRYGG 3 cut(s) 130, 151, 401
BstF5I GGATG 7 cut(s) 28, 379, 595, 786, 843, 883, 1357
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 3 cut(s) 310, 715, 898
BstMAI GTCTC 2 cut(s) 20, 910
BstMBI GATC 3 cut(s) 307, 712, 895
BstMWI GCNNNNNNNGC 6 cut(s) 75, 120, 592, 1074, 1168, 1313
BstNI CCWGG 1 cut(s) 791
BstNSI RCATGY 2 cut(s) 1091, 1220
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 789, 807
BstSLI GKGCMC 1 cut(s) 1113
BstV1I GCAGC 5 cut(s) 56, 120, 123, 1171, 1300
BstV2I GAAGAC 1 cut(s) 730
BstX2I RGATCY 1 cut(s) 895
BstXI CCANNNNNNTGG 1 cut(s) 1348
BstYI RGATCY 1 cut(s) 895
Bsu36I CCTNAGG 1 cut(s) 1080
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 3 cut(s) 586, 1133, 1178
BtgI CCRYGG 3 cut(s) 130, 151, 401
BtgZI GCGATG 1 cut(s) 18
BtsCI GGATG 7 cut(s) 28, 379, 595, 786, 843, 883, 1357
BtsIMutI CAGTG 2 cut(s) 330, 910
Cac8I GCNNGC 5 cut(s) 462, 987, 1169, 1173, 1275
CaiI CAGNNNCTG 2 cut(s) 899, 1313
CciI TCATGA 2 cut(s) 646, 736
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 688
Cfr13I GGNCC 6 cut(s) 504, 584, 908, 1094, 1131, 1235
Csp6I GTAC 2 cut(s) 241, 406
CviQI GTAC 2 cut(s) 241, 406
DdeI CTNAG 6 cut(s) 94, 455, 990, 1080, 1119, 1256
DinI GGCGCC 1 cut(s) 136
DpnI GATC 3 cut(s) 309, 714, 897
DpnII GATC 3 cut(s) 307, 712, 895
EaeI YGGCCR 1 cut(s) 1176
Ecl136II GAGCTC 2 cut(s) 781, 1255
Eco130I CCWWGG 1 cut(s) 130
Eco24I GRGCYC 2 cut(s) 783, 1257
Eco31I GGTCTC 1 cut(s) 910
Eco47I GGWCC 4 cut(s) 504, 908, 1094, 1235
Eco53kI GAGCTC 2 cut(s) 781, 1255
Eco81I CCTNAGG 1 cut(s) 1080
EcoICRI GAGCTC 2 cut(s) 781, 1255
EcoO109I RGGNCCY 3 cut(s) 504, 584, 1131
EcoRI GAATTC 2 cut(s) 221, 229
EcoRII CCWGG 1 cut(s) 789
EcoT14I CCWWGG 1 cut(s) 130
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 2 cut(s) 783, 1257
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 130
Esp3I CGTCTC 1 cut(s) 20
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 1 cut(s) 791
FauI CCCGC 3 cut(s) 453, 1061, 1242
FauNDI CATATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 307
Fnu4HI GCNGC 7 cut(s) 70, 109, 112, 465, 1160, 1176, 1314
FokI GGATG 7 cut(s) 35, 366, 582, 773, 830, 890, 1364
FriOI GRGCYC 2 cut(s) 783, 1257
Fsp4HI GCNGC 7 cut(s) 70, 109, 112, 465, 1160, 1176, 1314
FspBI CTAG 3 cut(s) 501, 759, 1098
GlaI GCGC 1 cut(s) 136
GluI GCNGC 7 cut(s) 70, 109, 112, 465, 1160, 1176, 1314
GsaI CCCAGC 1 cut(s) 798
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 3 cut(s) 586, 1133, 1178
HapII CCGG 3 cut(s) 437, 689, 809
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 135
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 696
HinfI GANTC 3 cut(s) 263, 747, 1360
HpaII CCGG 3 cut(s) 437, 689, 809
HphI GGTGA 2 cut(s) 373, 1069
Hpy166II GTNNAC 1 cut(s) 908
Hpy188III TCNNGA 9 cut(s) 226, 333, 541, 647, 737, 899, 1223, 1258, 1357
Hpy8I GTNNAC 1 cut(s) 908
HpyAV CCTTC 4 cut(s) 214, 255, 412, 1144
HpyCH4III ACNGT 5 cut(s) 377, 405, 670, 905, 1127
HpyCH4V TGCA 7 cut(s) 108, 212, 353, 620, 786, 1087, 1299
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 120, 592, 1074, 1168, 1313
HpyF3I CTNAG 6 cut(s) 94, 455, 990, 1080, 1119, 1256
Hsp92I GRCGYC 1 cut(s) 135
HspAI GCGC 1 cut(s) 135
KasI GGCGCC 1 cut(s) 134
Ksp22I TGATCA 1 cut(s) 307
Kzo9I GATC 3 cut(s) 307, 712, 895
LmnI GCTCC 5 cut(s) 451, 1028, 1246, 1252, 1310
Lsp1109I GCAGC 5 cut(s) 56, 120, 123, 1171, 1300
LweI GCATC 4 cut(s) 41, 604, 795, 862
MaeI CTAG 3 cut(s) 501, 759, 1098
MaeIII GTNAC 3 cut(s) 279, 368, 664
MalI GATC 3 cut(s) 309, 714, 897
MbiI CCGCTC 1 cut(s) 1249
MboI GATC 3 cut(s) 307, 712, 895
MboII GAAGA 8 cut(s) 164, 427, 436, 735, 843, 980, 983, 1057
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 4 cut(s) 86, 783, 1113, 1257
MluCI AATT 5 cut(s) 221, 229, 303, 573, 863
Mly113I GGCGCC 1 cut(s) 135
MmeI TCCRAC 2 cut(s) 417, 1329
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 3 cut(s) 9, 480, 1374
MslI CAYNNNNRTG 2 cut(s) 17, 708
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 3 cut(s) 437, 689, 809
MspR9I CCNGG 2 cut(s) 791, 809
Mva1269I GAATGC 2 cut(s) 840, 1299
MvaI CCWGG 1 cut(s) 791
MwoI GCNNNNNNNGC 6 cut(s) 75, 120, 592, 1074, 1168, 1313
NarI GGCGCC 1 cut(s) 135
NciI CCSGG 1 cut(s) 809
NcoI CCATGG 1 cut(s) 130
NdeI CATATG 1 cut(s) 349
NdeII GATC 3 cut(s) 307, 712, 895
NlaIV GGNNCC 8 cut(s) 136, 441, 506, 550, 585, 910, 1030, 1132
NmuCI GTSAC 2 cut(s) 368, 664
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 2 cut(s) 1091, 1220
NspV TTCGAA 2 cut(s) 341, 413
PagI TCATGA 2 cut(s) 646, 736
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PctI GAATGC 2 cut(s) 840, 1299
PfeI GAWTC 3 cut(s) 263, 747, 1360
PfoI TCCNGGA 1 cut(s) 807
PkrI GCNGC 7 cut(s) 71, 110, 113, 466, 1161, 1177, 1315
PluTI GGCGCC 1 cut(s) 138
PpuMI RGGWCCY 1 cut(s) 504
PshAI GACNNNNGTC 1 cut(s) 375
Psp124BI GAGCTC 2 cut(s) 783, 1257
Psp5II RGGWCCY 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 789
PspFI CCCAGC 1 cut(s) 794
PspGI CCWGG 1 cut(s) 789
PspN4I GGNNCC 8 cut(s) 136, 441, 506, 550, 585, 910, 1030, 1132
PspPI GGNCC 6 cut(s) 504, 584, 908, 1094, 1131, 1235
PspPPI RGGWCCY 1 cut(s) 504
PstNI CAGNNNCTG 2 cut(s) 899, 1313
PsuI RGATCY 1 cut(s) 895
RsaI GTAC 2 cut(s) 242, 407
RsaNI GTAC 2 cut(s) 241, 406
RseI CAYNNNNRTG 2 cut(s) 17, 708
SacI GAGCTC 2 cut(s) 783, 1257
SaqAI TTAA 3 cut(s) 9, 480, 1374
SatI GCNGC 7 cut(s) 70, 109, 112, 465, 1160, 1176, 1314
Sau3AI GATC 3 cut(s) 307, 712, 895
Sau96I GGNCC 6 cut(s) 504, 584, 908, 1094, 1131, 1235
ScrFI CCNGG 2 cut(s) 791, 809
SduI GDGCHC 4 cut(s) 86, 783, 1113, 1257
SfaNI GCATC 4 cut(s) 41, 604, 795, 862
SfoI GGCGCC 1 cut(s) 136
SfuI TTCGAA 2 cut(s) 341, 413
SinI GGWCC 4 cut(s) 504, 908, 1094, 1235
SmiMI CAYNNNNRTG 2 cut(s) 17, 708
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 5 cut(s) 221, 229, 303, 573, 863
SsiI CCGC 7 cut(s) 460, 464, 875, 1068, 1175, 1249, 1283
SspDI GGCGCC 1 cut(s) 134
SspMI CTAG 3 cut(s) 501, 759, 1098
SstI GAGCTC 2 cut(s) 783, 1257
StyD4I CCNGG 2 cut(s) 789, 807
StyI CCWWGG 1 cut(s) 130
TaaI ACNGT 5 cut(s) 377, 405, 670, 905, 1127
TaqI TCGA 5 cut(s) 261, 332, 341, 413, 1290
TaqII GACCGA 1 cut(s) 1082
TasI AATT 5 cut(s) 221, 229, 303, 573, 863
TauI GCSGC 2 cut(s) 467, 1178
TfiI GAWTC 3 cut(s) 263, 747, 1360
Tru1I TTAA 3 cut(s) 9, 480, 1374
Tru9I TTAA 3 cut(s) 9, 480, 1374
TscAI CASTG 2 cut(s) 330, 910
TseFI GTSAC 2 cut(s) 368, 664
TseI GCWGC 5 cut(s) 69, 108, 111, 1159, 1313
Tsp45I GTSAC 2 cut(s) 368, 664
TspDTI ATGAA 7 cut(s) 135, 566, 585, 635, 894, 981, 1058
TspGWI ACGGA 3 cut(s) 67, 168, 547
TspRI CASTG 2 cut(s) 330, 910
Vha464I CTTAAG 1 cut(s) 479
VpaK11BI GGWCC 4 cut(s) 504, 908, 1094, 1235
XapI RAATTY 4 cut(s) 221, 229, 573, 863
XceI RCATGY 2 cut(s) 1091, 1220
XcmI CCANNNNNNNNNTGG 2 cut(s) 1151, 1209
XspI CTAG 3 cut(s) 501, 759, 1098
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.