AT5G58020

Rtf2 RING-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014164
Physical Location & Seq
Forward (+)
23485496 .. 23486818
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58020.1

Sequence Viewer

Length: 1065 bp
ATGCATATCCGGCGCCAGATCTTCGTTAAATCTCCTGACTGTCAAAAAGTCGTCGCTCTGCAATTGGATCCTGCGCAATCTCTATTAACTCTTTCGGGTATCACTTCCTTACTCGAATCGTCGCAGCGTATATCGTTTTCGGCTTGCTCGATTACTCTAGATGGGAAGCTTTTGAATGGCTCTACACGGATCCAAGTATCGAAGCTTCCTTCTGTATCCATGCTCACTCTTTTCCCTCGCCTCCGTGGAGGTGGAGGAGATGGTGGGGCAACGGGAGCCGAGTCTCGCGATTGCTATCTCAATATGTACGCGGAGAAGAAACCTGACAAGGTTGATCCTAATGAGCAGAGGCTCTCCAAATGGTTGAATTGTGCTTTGTCTAACGAGCCTTTGGCTGAGCCGTGCGTGATTGATCTCCTTGGGAATTTGTTCAATAAAGAAGTTTTGGTGCACGCTTTGTTGTCTAAGAGGTTGCCTAAACAATTCTCGTACATTAAGGGTTTGAAAGATATGGTGAATATTAAGCTTACGCCTGTTGCTGGTTCTGATGGTTCATCGCAAGATACGACTAGTGCACAGTTTCAGTGTCCGGTCTCAGGGCTTGAATTCAATGGAAAGTACAAGTTTTTCGCTTTGAGGGGATGTGGACATGTGATGAGCGCAAAGGCGTTGAAGGAAGTGAAGTCTTCTTCTTGTTTGGTGTGTCATGCGGATGTCAAAGATTCTGATAAGATTGTGATCAATGGTACTGAGGAGGAAGTAGATTTGTTGAGGGAGAGGATGGAGGAGGAGAAAGCTAAGCTGAGAGAGAAGAAAGGTGTCTCAAAGAAGAGTAAGAACGGTGCTGCTGTGGTGGCTGATACAGGTGCGAAGGTCGCAAAGAGACAGATAGAGGATGGGAATGTCAATGGCAATGGTATAACTGTGAAGAAATTCAAGGCGGCGGATAAAGTGCCGGTTAATGCTACTAAGGAAGTCTATGCTTCTCTCTTTACTTCCTCTAAGAAGAAGTCTGATTTCAGGGAGACTTACTCTTGCAGGTCACTTCCTCTCGGCAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

354

Amino Acids

38.6

Weight (kDa)

9.35

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 84 - 337 6.2e-83 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 1029
AccB1I GGYRCC 1 cut(s) 12
AccII CGCG 2 cut(s) 288, 311
AciI CCGC 4 cut(s) 311, 710, 941, 944
AclWI GGATC 5 cut(s) 62, 75, 184, 197, 329
AcsI RAATTY 3 cut(s) 424, 605, 932
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 4 cut(s) 308, 491, 620, 748
AfiI CCNNNNNNNGG 2 cut(s) 539, 596
AflIII ACRYGT 1 cut(s) 649
AgsI TTSAA 8 cut(s) 175, 367, 433, 505, 605, 610, 673, 937
AhlI ACTAGT 1 cut(s) 569
AluBI AGCT 5 cut(s) 169, 205, 526, 797, 802
AluI AGCT 5 cut(s) 169, 205, 526, 797, 802
Alw21I GWGCWC 2 cut(s) 453, 577
Alw26I GTCTC 5 cut(s) 288, 598, 826, 877, 1019
Alw44I GTGCAC 2 cut(s) 449, 573
AlwI GGATC 5 cut(s) 62, 75, 184, 197, 329
ApaLI GTGCAC 2 cut(s) 449, 573
ApeKI GCWGC 2 cut(s) 124, 845
ApoI RAATTY 3 cut(s) 424, 605, 932
Asp700I GAANNNNTTC 2 cut(s) 428, 932
AspLEI GCGC 3 cut(s) 15, 76, 662
AsuHPI GGTGA 1 cut(s) 526
BaeGI GKGCMC 2 cut(s) 453, 577
BamHI GGATCC 2 cut(s) 67, 189
BanI GGYRCC 1 cut(s) 12
BarI GAAGNNNNNNTAC 2 cut(s) 189, 221
BbsI GAAGAC 1 cut(s) 678
Bbv12I GWGCWC 2 cut(s) 453, 577
BbvI GCAGC 2 cut(s) 136, 832
BccI CCATC 5 cut(s) 155, 254, 542, 775, 890
BceAI ACGGC 1 cut(s) 385
BciVI GTATCC 1 cut(s) 226
BclI TGATCA 1 cut(s) 738
BcoDI GTCTC 5 cut(s) 288, 598, 826, 877, 1019
BcuI ACTAGT 1 cut(s) 569
BfaI CTAG 2 cut(s) 158, 570
BfoI RGCGCY 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 1029
BfuI GTATCC 1 cut(s) 226
BglII AGATCT 1 cut(s) 18
BisI GCNGC 3 cut(s) 125, 846, 942
BlpI GCTNAGC 2 cut(s) 396, 798
BlsI GCNGC 3 cut(s) 126, 847, 943
BmiI GGNNCC 4 cut(s) 14, 69, 191, 277
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 1016, 1048
Bpu1102I GCTNAGC 2 cut(s) 396, 798
BsaBI GATNNNNATC 2 cut(s) 294, 737
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 2 cut(s) 244, 418
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 596
Bse118I RCCGGY 1 cut(s) 955
Bse3DI GCAATG 1 cut(s) 919
Bse8I GATNNNNATC 2 cut(s) 294, 737
BseDI CCNNGG 2 cut(s) 244, 418
BseGI GGATG 4 cut(s) 647, 718, 786, 901
BseJI GATNNNNATC 2 cut(s) 294, 737
BseLI CCNNNNNNNGG 2 cut(s) 539, 596
BseMI GCAATG 1 cut(s) 919
BseMII CTCAG 4 cut(s) 387, 609, 741, 794
BseRI GAGGAG 4 cut(s) 270, 767, 800, 803
BseSI GKGCMC 2 cut(s) 453, 577
BseXI GCAGC 2 cut(s) 136, 832
Bsh1236I CGCG 2 cut(s) 288, 311
BshNI GGYRCC 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 453, 577
BsiSI CCGG 3 cut(s) 10, 590, 956
BslI CCNNNNNNNGG 2 cut(s) 539, 596
BsmAI GTCTC 5 cut(s) 288, 598, 826, 877, 1019
Bso31I GGTCTC 1 cut(s) 598
Bsp1286I GDGCHC 2 cut(s) 453, 577
Bsp143I GATC 6 cut(s) 18, 67, 189, 334, 412, 738
Bsp1720I GCTNAGC 2 cut(s) 396, 798
Bsp68I TCGCGA 1 cut(s) 288
BspACI CCGC 4 cut(s) 311, 710, 941, 944
BspCNI CTCAG 4 cut(s) 388, 608, 742, 795
BspFNI CGCG 2 cut(s) 288, 311
BspLI GGNNCC 4 cut(s) 14, 69, 191, 277
BspMI ACCTGC 1 cut(s) 1029
BspPI GGATC 5 cut(s) 62, 75, 184, 197, 329
BspT107I GGYRCC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 919
BsrFI RCCGGY 1 cut(s) 955
BssAI RCCGGY 1 cut(s) 955
BssECI CCNNGG 2 cut(s) 244, 418
BssMI GATC 6 cut(s) 18, 67, 189, 334, 412, 738
BssNI GRCGYC 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 4 cut(s) 41, 579, 842, 925
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 145, 453
BstDEI CTNAG 8 cut(s) 396, 465, 595, 750, 798, 803, 969, 1002
BstDSI CCRYGG 1 cut(s) 244
BstF5I GGATG 4 cut(s) 647, 718, 786, 901
BstFNI CGCG 2 cut(s) 288, 311
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 3 cut(s) 15, 76, 662
BstKTI GATC 6 cut(s) 21, 70, 192, 337, 415, 741
BstMAI GTCTC 5 cut(s) 288, 598, 826, 877, 1019
BstMBI GATC 6 cut(s) 18, 67, 189, 334, 412, 738
BstMWI GCNNNNNNNGC 4 cut(s) 10, 275, 854, 875
BstNSI RCATGY 1 cut(s) 653
BstSLI GKGCMC 2 cut(s) 453, 577
BstUI CGCG 2 cut(s) 288, 311
BstV1I GCAGC 2 cut(s) 136, 832
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 3 cut(s) 18, 67, 189
BstYI RGATCY 3 cut(s) 18, 67, 189
BsuI GTATCC 1 cut(s) 226
BtgI CCRYGG 1 cut(s) 244
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 4 cut(s) 647, 718, 786, 901
BtsIMutI CAGTG 1 cut(s) 590
BtuMI TCGCGA 1 cut(s) 288
BveI ACCTGC 1 cut(s) 1029
Cac8I GCNNGC 2 cut(s) 145, 453
CfoI GCGC 3 cut(s) 15, 76, 662
Cfr10I RCCGGY 1 cut(s) 955
Csp6I GTAC 4 cut(s) 307, 490, 619, 747
CviAII CATG 3 cut(s) 220, 650, 707
CviQI GTAC 4 cut(s) 307, 490, 619, 747
DdeI CTNAG 8 cut(s) 396, 465, 595, 750, 798, 803, 969, 1002
DinI GGCGCC 1 cut(s) 14
DpnI GATC 6 cut(s) 20, 69, 191, 336, 414, 740
DpnII GATC 6 cut(s) 18, 67, 189, 334, 412, 738
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
EciI GGCGGA 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 598
EcoRI GAATTC 1 cut(s) 605
EcoT14I CCWWGG 1 cut(s) 418
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 3 cut(s) 223, 653, 710
FaiI YATR 9 cut(s) 6, 131, 221, 305, 512, 651, 708, 920, 981
FatI CATG 3 cut(s) 219, 649, 706
FbaI TGATCA 1 cut(s) 738
Fnu4HI GCNGC 3 cut(s) 125, 846, 942
FokI GGATG 4 cut(s) 654, 725, 793, 908
Fsp4HI GCNGC 3 cut(s) 125, 846, 942
FspBI CTAG 2 cut(s) 158, 570
FspI TGCGCA 1 cut(s) 75
GlaI GCGC 3 cut(s) 14, 75, 661
GluI GCNGC 3 cut(s) 125, 846, 942
HaeII RGCGCY 1 cut(s) 16
HapII CCGG 3 cut(s) 10, 590, 956
HhaI GCGC 3 cut(s) 15, 76, 662
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 3 cut(s) 223, 653, 710
Hin6I GCGC 3 cut(s) 13, 74, 660
HinP1I GCGC 3 cut(s) 13, 74, 660
HindIII AAGCTT 3 cut(s) 167, 203, 524
HinfI GANTC 3 cut(s) 116, 281, 722
HpaII CCGG 3 cut(s) 10, 590, 956
HphI GGTGA 1 cut(s) 526
Hpy166II GTNNAC 3 cut(s) 451, 575, 647
Hpy188I TCNGA 3 cut(s) 547, 727, 1015
Hpy188III TCNNGA 3 cut(s) 35, 158, 287
Hpy8I GTNNAC 3 cut(s) 451, 575, 647
Hpy99I CGWCG 2 cut(s) 56, 124
HpyAV CCTTC 3 cut(s) 219, 667, 865
HpyCH4III ACNGT 4 cut(s) 41, 579, 842, 925
HpyCH4V TGCA 5 cut(s) 4, 61, 451, 575, 1038
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 275, 854, 875
HpyF3I CTNAG 8 cut(s) 396, 465, 595, 750, 798, 803, 969, 1002
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 3 cut(s) 223, 653, 710
HspAI GCGC 3 cut(s) 13, 74, 660
KasI GGCGCC 1 cut(s) 12
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 6 cut(s) 18, 67, 189, 334, 412, 738
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 2 cut(s) 136, 832
MaeI CTAG 2 cut(s) 158, 570
MaeIII GTNAC 1 cut(s) 1041
MalI GATC 6 cut(s) 20, 69, 191, 336, 414, 740
MboI GATC 6 cut(s) 18, 67, 189, 334, 412, 738
MboII GAAGA 8 cut(s) 13, 328, 678, 681, 823, 841, 940, 1018
MfeI CAATTG 1 cut(s) 62
MflI RGATCY 3 cut(s) 18, 67, 189
MhlI GDGCHC 2 cut(s) 453, 577
MluCI AATT 7 cut(s) 62, 367, 424, 482, 605, 932, 1060
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 428, 932
MseI TTAA 5 cut(s) 27, 86, 495, 522, 960
MslI CAYNNNNRTG 1 cut(s) 711
MspI CCGG 3 cut(s) 10, 590, 956
MunI CAATTG 1 cut(s) 62
MvnI CGCG 2 cut(s) 288, 311
MwoI GCNNNNNNNGC 4 cut(s) 10, 275, 854, 875
NarI GGCGCC 1 cut(s) 13
NdeII GATC 6 cut(s) 18, 67, 189, 334, 412, 738
NlaIII CATG 3 cut(s) 223, 653, 710
NlaIV GGNNCC 4 cut(s) 14, 69, 191, 277
NmeAIII GCCGAG 2 cut(s) 304, 1032
NmuCI GTSAC 1 cut(s) 1041
NruI TCGCGA 1 cut(s) 288
NsbI TGCGCA 1 cut(s) 75
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 653
PciI ACATGT 1 cut(s) 649
PcsI WCGNNNNNNNCGW 2 cut(s) 146, 563
PdmI GAANNNNTTC 2 cut(s) 428, 932
PfeI GAWTC 2 cut(s) 116, 722
PkrI GCNGC 3 cut(s) 126, 847, 943
PleI GAGTC 1 cut(s) 289
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 289
PscI ACATGT 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 14, 69, 191, 277
PsuI RGATCY 3 cut(s) 18, 67, 189
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 4 cut(s) 308, 491, 620, 748
RsaNI GTAC 4 cut(s) 307, 490, 619, 747
RseI CAYNNNNRTG 1 cut(s) 711
SaqAI TTAA 5 cut(s) 27, 86, 495, 522, 960
SatI GCNGC 3 cut(s) 125, 846, 942
Sau3AI GATC 6 cut(s) 18, 67, 189, 334, 412, 738
SchI GAGTC 1 cut(s) 290
SduI GDGCHC 2 cut(s) 453, 577
SfoI GGCGCC 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 711
SpeI ACTAGT 1 cut(s) 569
Sse9I AATT 7 cut(s) 62, 367, 424, 482, 605, 932, 1060
SsiI CCGC 4 cut(s) 311, 710, 941, 944
SspDI GGCGCC 1 cut(s) 12
SspI AATATT 1 cut(s) 520
SspMI CTAG 2 cut(s) 158, 570
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 4 cut(s) 41, 579, 842, 925
TaqI TCGA 3 cut(s) 114, 149, 200
TasI AATT 7 cut(s) 62, 367, 424, 482, 605, 932, 1060
TatI WGTACW 1 cut(s) 618
TauI GCSGC 1 cut(s) 944
TfiI GAWTC 2 cut(s) 116, 722
Tru1I TTAA 5 cut(s) 27, 86, 495, 522, 960
Tru9I TTAA 5 cut(s) 27, 86, 495, 522, 960
TscAI CASTG 1 cut(s) 590
TseFI GTSAC 1 cut(s) 1041
TseI GCWGC 2 cut(s) 124, 845
Tsp45I GTSAC 1 cut(s) 1041
TspDTI ATGAA 1 cut(s) 543
TspGWI ACGGA 2 cut(s) 202, 233
TspRI CASTG 1 cut(s) 590
VneI GTGCAC 2 cut(s) 449, 573
XapI RAATTY 3 cut(s) 424, 605, 932
XbaI TCTAGA 1 cut(s) 157
XceI RCATGY 1 cut(s) 653
XmnI GAANNNNTTC 2 cut(s) 428, 932
XspI CTAG 2 cut(s) 158, 570
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.