Rw2G001210

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014164
Physical Location & Seq
Forward (+)
1028358 .. 1029945
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001210.1

Sequence Viewer

Length: 1143 bp
ATGCATTCCAAGCCCCAAACTTTCCAAATCTTCCTCGAATCCCCACAACTCCAAATCCCAACCCAAACCCTAACCCTATCCCCAATTCAAACCCCAACTCTCCACCACCTCAAGCTTTCTCTTTTCTCCCAAACCCTTCCCTCCCTCTACTTCACCCTCAATGGAAAGCCCTTATTCGACTCCACCCCTCTCAGTTCCGAAATTTCTCCCCTTTCGACCCTAATTTTGCAATTCAGAGCTCCCGGCGGAGGCGGCGATGGCGGCGCCACCGGGGCCGAGTCGAGGGATTGCTATCTCAACATGTACGCCGAGAAAAAGCCTGATAAGGTCGACCCGTACGAGCAGAGACTCTCCAAGTTTCTGAACTGCGCGCTTTCCAATGAGCCATTGAAGGATCCTTGCGTTGTGGATTTGCTCGGGAATGTGTTCAATAAGGAGCCTCTGGTTGAGGCCTTGTTGGGGAAGAAGGTGCCGAAAGCTTTCGGGCACATTAAGGGTTTAAGGGACATGGTTCCTGTGCATTTTACTTCGATTAACTTGCCCGAATCGGAGTCTCAGGCTACGGCCACCGGACCGAGGTTTCAGTGCCCCATTACGGGGCTGGAGTTTAATGGGAAGTCTAAGTTTCTGGCGTTGAGGAGTTGCGGGCATGTGTTGAGTGCAAAGGCATTGAAGGAGGTGAAGTCCTCGGCTTGCCTTGTGTGTCATGTGGGGTTTTCGGAGGAGGATAAGATTGTGATAAATGGGAATGAGGAGGAGGTCGCGGCGCTGAGGGAGAGGATGGAGTCTGAGAAGGCGAAATCGAGGGGGAAGAAGGTGAAGAAGACAAAAAATGGGGAGGCGGTTGGGAATGCGGAGGAGGAGAGTGTGGAGGGTTCGAGGTTGAGTGGTACGAAGCATGGCATTGATGTGAAGGGTGTGGAGAAGGCTTCGGTGAAGGTGGAGGGGAATGGAAAGGTTGGGAATGGTGGGGAGAATGTGAAGGGTGTGAGTAATGGTCCTGCAGTGAAGAAGTTTAAGGCGGTGGATGCAGCCCCAGCTAATGCTACTAAGGAAGTGTATGCTTCGATTTTCACTTCGTCGAGCAAGAAGAAGTTTAAGGAGACATATTCTTGTAGATCTCTCCCACTTGGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

380

Amino Acids

41.01

Weight (kDa)

9.13

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 83 - 365 2.7e-75 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 263, 469
AccI GTMKAC 1 cut(s) 330
AccII CGCG 2 cut(s) 371, 764
AciI CCGC 8 cut(s) 246, 252, 261, 645, 764, 842, 854, 1022
AclWI GGATC 2 cut(s) 389, 402
AcoI YGGCCR 1 cut(s) 564
AcsI RAATTY 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 3 cut(s) 305, 338, 892
AfiI CCNNNNNNNGG 7 cut(s) 248, 282, 459, 576, 595, 596, 597
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 4 cut(s) 89, 391, 430, 673
AluBI AGCT 4 cut(s) 115, 239, 479, 1040
AluI AGCT 4 cut(s) 115, 239, 479, 1040
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 3 cut(s) 340, 558, 1097
AlwI GGATC 2 cut(s) 389, 402
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 3 cut(s) 273, 450, 564
ApeKI GCWGC 1 cut(s) 1031
ApoI RAATTY 1 cut(s) 201
ArsI GACNNNNNNTTYG 2 cut(s) 208, 240
Asp700I GAANNNNTTC 2 cut(s) 425, 479
AspLEI GCGC 4 cut(s) 266, 371, 373, 769
AspS9I GGNCC 3 cut(s) 273, 572, 998
AsuC2I CCSGG 2 cut(s) 243, 271
AsuHPI GGTGA 4 cut(s) 145, 691, 829, 946
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 2 cut(s) 572, 998
BaeGI GKGCMC 2 cut(s) 489, 590
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 2 cut(s) 263, 469
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 830
Bbv12I GWGCWC 1 cut(s) 241
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 1 cut(s) 1043
BccI CCATC 2 cut(s) 251, 775
BceAI ACGGC 1 cut(s) 579
BcgI CGANNNNNNTGC 2 cut(s) 520, 554
BcnI CCSGG 2 cut(s) 243, 271
BcoDI GTCTC 3 cut(s) 340, 558, 1097
BfmI CTRYAG 1 cut(s) 1002
BfoI RGCGCY 2 cut(s) 267, 770
BglI GCCNNNNNGGC 1 cut(s) 272
BglII AGATCT 1 cut(s) 1118
BisI GCNGC 4 cut(s) 253, 262, 765, 1032
BlsI GCNGC 4 cut(s) 254, 263, 766, 1033
Bme1390I CCNGG 2 cut(s) 243, 271
Bme18I GGWCC 2 cut(s) 572, 998
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 3 cut(s) 273, 572, 998
BmiI GGNNCC 6 cut(s) 265, 274, 396, 438, 471, 513
BmrFI CCNGG 2 cut(s) 243, 271
BmsI GCATC 1 cut(s) 1018
BpiI GAAGAC 1 cut(s) 830
BpmI CTGGAG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 243, 271
BsaBI GATNNNNATC 1 cut(s) 291
BsaHI GRCGYC 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 270, 575, 687
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 2 cut(s) 668, 698
Bsc4I CCNNNNNNNGG 7 cut(s) 248, 282, 459, 576, 595, 596, 597
Bse8I GATNNNNATC 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 270, 575, 687
BseGI GGATG 2 cut(s) 786, 1033
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 7 cut(s) 248, 282, 459, 576, 595, 596, 597
BseMII CTCAG 4 cut(s) 205, 569, 761, 780
BsePI GCGCGC 1 cut(s) 369
BseRI GAGGAG 6 cut(s) 652, 737, 767, 770, 872, 875
BseSI GKGCMC 2 cut(s) 489, 590
BseXI GCAGC 1 cut(s) 1043
BseYI CCCAGC 1 cut(s) 1036
Bsh1236I CGCG 2 cut(s) 371, 764
BshFI GGCC 3 cut(s) 275, 452, 566
BshNI GGYRCC 2 cut(s) 263, 469
BsiHKAI GWGCWC 1 cut(s) 241
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 243, 270, 570
BsiWI CGTACG 1 cut(s) 336
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 7 cut(s) 248, 282, 459, 576, 595, 596, 597
BsmAI GTCTC 3 cut(s) 340, 558, 1097
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 2 cut(s) 4, 856
BsnI GGCC 3 cut(s) 275, 452, 566
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 3 cut(s) 241, 489, 590
Bsp143I GATC 2 cut(s) 394, 1118
BspACI CCGC 8 cut(s) 246, 252, 261, 645, 764, 842, 854, 1022
BspANI GGCC 3 cut(s) 275, 452, 566
BspCNI CTCAG 4 cut(s) 204, 568, 762, 781
BspFNI CGCG 2 cut(s) 371, 764
BspLI GGNNCC 6 cut(s) 265, 274, 396, 438, 471, 513
BspMAI CTGCAG 1 cut(s) 1006
BspPI GGATC 2 cut(s) 389, 402
BspT107I GGYRCC 2 cut(s) 263, 469
BssECI CCNNGG 3 cut(s) 270, 575, 687
BssHII GCGCGC 1 cut(s) 369
BssMI GATC 2 cut(s) 394, 1118
BssNI GRCGYC 1 cut(s) 264
BstACI GRCGYC 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 371, 647, 694
BstDEI CTNAG 6 cut(s) 191, 555, 621, 770, 789, 1050
BstF5I GGATG 2 cut(s) 786, 1033
BstFNI CGCG 2 cut(s) 371, 764
BstH2I RGCGCY 2 cut(s) 267, 770
BstHHI GCGC 4 cut(s) 266, 371, 373, 769
BstKTI GATC 2 cut(s) 397, 1121
BstMAI GTCTC 3 cut(s) 340, 558, 1097
BstMBI GATC 2 cut(s) 394, 1118
BstMWI GCNNNNNNNGC 7 cut(s) 10, 252, 258, 261, 272, 1028, 1037
BstNSI RCATGY 2 cut(s) 304, 653
BstSCI CCNGG 2 cut(s) 241, 269
BstSFI CTRYAG 1 cut(s) 1002
BstSLI GKGCMC 2 cut(s) 489, 590
BstUI CGCG 2 cut(s) 371, 764
BstV1I GCAGC 1 cut(s) 1043
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 2 cut(s) 394, 1118
BstYI RGATCY 2 cut(s) 394, 1118
BsuRI GGCC 3 cut(s) 275, 452, 566
BtgZI GCGATG 1 cut(s) 270
BtsCI GGATG 2 cut(s) 786, 1033
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 2 cut(s) 590, 1011
Cac8I GCNNGC 3 cut(s) 371, 647, 694
CfoI GCGC 4 cut(s) 266, 371, 373, 769
Cfr13I GGNCC 3 cut(s) 273, 572, 998
CpoI CGGWCCG 1 cut(s) 572
Csp6I GTAC 3 cut(s) 304, 337, 891
CspI CGGWCCG 1 cut(s) 572
CviAII CATG 5 cut(s) 301, 508, 650, 707, 899
CviQI GTAC 3 cut(s) 304, 337, 891
DdeI CTNAG 6 cut(s) 191, 555, 621, 770, 789, 1050
DinI GGCGCC 1 cut(s) 265
DpnI GATC 2 cut(s) 396, 1120
DpnII GATC 2 cut(s) 394, 1118
EaeI YGGCCR 1 cut(s) 564
EciI GGCGGA 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 572, 998
Eco53kI GAGCTC 1 cut(s) 239
Eco88I CYCGRG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 239
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 241
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
FaeI CATG 5 cut(s) 304, 511, 653, 710, 902
FaiI YATR 7 cut(s) 302, 509, 651, 708, 900, 1062, 1108
FaqI GGGAC 1 cut(s) 518
FatI CATG 5 cut(s) 300, 507, 649, 706, 898
FauI CCCGC 1 cut(s) 638
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 4 cut(s) 253, 262, 765, 1032
FokI GGATG 2 cut(s) 793, 1040
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 4 cut(s) 253, 262, 765, 1032
GlaI GCGC 4 cut(s) 265, 370, 372, 768
GluI GCNGC 4 cut(s) 253, 262, 765, 1032
GsaI CCCAGC 1 cut(s) 1040
GsuI CTGGAG 1 cut(s) 623
HaeII RGCGCY 2 cut(s) 267, 770
HaeIII GGCC 3 cut(s) 275, 452, 566
HapII CCGG 3 cut(s) 243, 270, 570
HhaI GCGC 4 cut(s) 266, 371, 373, 769
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 5 cut(s) 304, 511, 653, 710, 902
Hin6I GCGC 4 cut(s) 264, 369, 371, 767
HinP1I GCGC 4 cut(s) 264, 369, 371, 767
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 2 cut(s) 113, 477
HinfI GANTC 7 cut(s) 38, 179, 278, 348, 545, 551, 785
HpaII CCGG 3 cut(s) 243, 270, 570
HphI GGTGA 4 cut(s) 145, 691, 829, 946
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 6 cut(s) 199, 236, 363, 550, 721, 790
Hpy188III TCNNGA 1 cut(s) 418
Hpy8I GTNNAC 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 1084
HpyCH4V TGCA 6 cut(s) 4, 229, 520, 662, 1004, 1031
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 252, 258, 261, 272, 1028, 1037
HpyF3I CTNAG 6 cut(s) 191, 555, 621, 770, 789, 1050
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 5 cut(s) 304, 511, 653, 710, 902
HspAI GCGC 4 cut(s) 264, 369, 371, 767
KasI GGCGCC 1 cut(s) 263
Kzo9I GATC 2 cut(s) 394, 1118
LmnI GCTCC 2 cut(s) 244, 436
Lsp1109I GCAGC 1 cut(s) 1043
LweI GCATC 1 cut(s) 1018
MalI GATC 2 cut(s) 396, 1120
MboI GATC 2 cut(s) 394, 1118
MboII GAAGA 7 cut(s) 22, 475, 823, 832, 835, 1021, 1102
MflI RGATCY 2 cut(s) 394, 1118
MhlI GDGCHC 3 cut(s) 241, 489, 590
MluCI AATT 4 cut(s) 84, 201, 222, 230
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 5 cut(s) 173, 287, 342, 560, 794
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 425, 479
MseI TTAA 6 cut(s) 492, 500, 534, 609, 1017, 1098
MslI CAYNNNNRTG 1 cut(s) 908
MspI CCGG 3 cut(s) 243, 270, 570
MspR9I CCNGG 2 cut(s) 243, 271
Mva1269I GAATGC 2 cut(s) 4, 856
MvnI CGCG 2 cut(s) 371, 764
MwoI GCNNNNNNNGC 7 cut(s) 10, 252, 258, 261, 272, 1028, 1037
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 243, 271
NdeII GATC 2 cut(s) 394, 1118
NlaIII CATG 5 cut(s) 304, 511, 653, 710, 902
NlaIV GGNNCC 6 cut(s) 265, 274, 396, 438, 471, 513
NmeAIII GCCGAG 3 cut(s) 301, 334, 668
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 304, 653
PauI GCGCGC 1 cut(s) 369
PceI AGGCCT 1 cut(s) 452
PciI ACATGT 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 2 cut(s) 4, 856
PdmI GAANNNNTTC 2 cut(s) 425, 479
PfeI GAWTC 2 cut(s) 38, 545
Pfl23II CGTACG 1 cut(s) 336
PkrI GCNGC 4 cut(s) 254, 263, 766, 1033
PleI GAGTC 5 cut(s) 173, 286, 342, 559, 793
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 5 cut(s) 173, 286, 342, 559, 793
PscI ACATGT 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 241
PspFI CCCAGC 1 cut(s) 1036
PspLI CGTACG 1 cut(s) 336
PspN4I GGNNCC 6 cut(s) 265, 274, 396, 438, 471, 513
PspPI GGNCC 3 cut(s) 273, 572, 998
PstI CTGCAG 1 cut(s) 1006
PsuI RGATCY 2 cut(s) 394, 1118
PteI GCGCGC 1 cut(s) 369
RsaI GTAC 3 cut(s) 305, 338, 892
RsaNI GTAC 3 cut(s) 304, 337, 891
RseI CAYNNNNRTG 1 cut(s) 908
Rsr2I CGGWCCG 1 cut(s) 572
RsrII CGGWCCG 1 cut(s) 572
SacI GAGCTC 1 cut(s) 241
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 6 cut(s) 492, 500, 534, 609, 1017, 1098
SatI GCNGC 4 cut(s) 253, 262, 765, 1032
Sau3AI GATC 2 cut(s) 394, 1118
Sau96I GGNCC 3 cut(s) 273, 572, 998
SchI GAGTC 5 cut(s) 173, 287, 342, 560, 794
ScrFI CCNGG 2 cut(s) 243, 271
SduI GDGCHC 3 cut(s) 241, 489, 590
SfaNI GCATC 1 cut(s) 1018
SfcI CTRYAG 1 cut(s) 1002
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 2 cut(s) 572, 998
SmiMI CAYNNNNRTG 1 cut(s) 908
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 84, 201, 222, 230
SseBI AGGCCT 1 cut(s) 452
SsiI CCGC 8 cut(s) 246, 252, 261, 645, 764, 842, 854, 1022
SspDI GGCGCC 1 cut(s) 263
SstI GAGCTC 1 cut(s) 241
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 2 cut(s) 241, 269
TaqII GACCGA 1 cut(s) 589
TasI AATT 4 cut(s) 84, 201, 222, 230
TauI GCSGC 3 cut(s) 255, 264, 767
TfiI GAWTC 2 cut(s) 38, 545
Tru1I TTAA 6 cut(s) 492, 500, 534, 609, 1017, 1098
Tru9I TTAA 6 cut(s) 492, 500, 534, 609, 1017, 1098
TscAI CASTG 2 cut(s) 590, 1011
TseI GCWGC 1 cut(s) 1031
TspRI CASTG 2 cut(s) 590, 1011
VpaK11BI GGWCC 2 cut(s) 572, 998
XapI RAATTY 1 cut(s) 201
XceI RCATGY 2 cut(s) 304, 653
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 2 cut(s) 425, 479
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.