Rmu_ssc0000355.1_g000028

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000355.1
OG0014164
Physical Location & Seq
Reverse (-)
116364 .. 117509
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000355.1_g000028.1.cds

Sequence Viewer

Length: 1146 bp
atgcattccaagccccaaactttccaaatcttcctcgaatccccacaactccaaatcccaacccaaaccctaaccctatccccaattcaaaccccaactctccaccacctcaagctttctcttttctcccaaacccttccctccctctacttcaccctcaatggaaagcccttattcgactccaccccgcttctcagttccgaaatttctcccctttcgactctaattttgcaattcagagctcccggcggaggcggcgatggcggcgccaccggggccgagtcaagggattgctatctcaacatgtacgccgagaaaaagcctgataaggtcgacccgtacgagcagagactctccaagtttctgaactgcgcgctttccaatgagccattgaaggatccttgcgttgtggatttgcttgggaatgtgttcaataaggagcctctggttgaggccttgttggggaagaaggtgccgaaagctttcgggcacattaagggtttaagggacatggttcctgtgcattttacttcgattaacttgcccgaatcggagtctcaggccacggccaccgggccgaggtttcagtgccccattacggggctggagtttaatgggaagtctaagtttctggcgttgaggagttgcgggcatgtgttgagtgcaaaggcattgaaggaggtgaagtcctcggcttgccttgtgtgtcatgtggggttttcggaggaggataagattgtgataaatgggaatgaggaggaggtcgaggcgctgagggagaggatggagtctgagaaggcgaaatcgagggggaagaaggtgaagaagacgaaaaatggggagggggttgggaatgcggaggaggagggtgtggagggttcgaggttgagtggtacgaagcatggcattgatgtgaagggtgtggagaaggcttcggcgaaggtggaggggaatggaaaggttgggaatggtggggagaatgtgaagggtgtgagtaatggtcctgcggtgaagaagtttaaggcggtggatgcagccccagctaatgctactaaggaagtgtatgcttcgattttcacttcgtcgagcaagaagaagtttaaggagacatattcttgtagatctctcccacttggtagaaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

381

Amino Acids

41.1

Weight (kDa)

9.06

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 266, 472
AccI GTMKAC 1 cut(s) 333
AccII CGCG 1 cut(s) 374
AciI CCGC 8 cut(s) 188, 249, 255, 264, 648, 857, 1007, 1025
AclWI GGATC 2 cut(s) 392, 405
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 3 cut(s) 308, 341, 895
AfiI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 89, 394, 433, 676
AluBI AGCT 4 cut(s) 115, 242, 482, 1043
AluI AGCT 4 cut(s) 115, 242, 482, 1043
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 343, 561, 1100
AlwI GGATC 2 cut(s) 392, 405
AoxI GGCC 5 cut(s) 276, 453, 561, 567, 575
ApeKI GCWGC 1 cut(s) 1034
ApoI RAATTY 1 cut(s) 204
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 2 cut(s) 428, 482
AspLEI GCGC 4 cut(s) 269, 374, 376, 772
AspS9I GGNCC 3 cut(s) 276, 575, 1001
AsuC2I CCSGG 3 cut(s) 246, 274, 574
AsuHPI GGTGA 4 cut(s) 145, 694, 832, 1021
AvaII GGWCC 1 cut(s) 1001
BaeGI GKGCMC 2 cut(s) 492, 593
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 2 cut(s) 266, 472
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 833
Bbv12I GWGCWC 1 cut(s) 244
BbvCI CCTCAGC 1 cut(s) 773
BbvI GCAGC 1 cut(s) 1046
BccI CCATC 2 cut(s) 254, 778
BceAI ACGGC 1 cut(s) 582
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BcnI CCSGG 3 cut(s) 246, 274, 574
BcoDI GTCTC 3 cut(s) 343, 561, 1100
BfoI RGCGCY 2 cut(s) 270, 773
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 1121
BisI GCNGC 3 cut(s) 256, 265, 1035
BlsI GCNGC 3 cut(s) 257, 266, 1036
Bme1390I CCNGG 3 cut(s) 246, 274, 574
Bme18I GGWCC 1 cut(s) 1001
BmgT120I GGNCC 3 cut(s) 276, 575, 1001
BmiI GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
BmrFI CCNGG 3 cut(s) 246, 274, 574
BmsI GCATC 1 cut(s) 1021
BpiI GAAGAC 1 cut(s) 833
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 3 cut(s) 246, 274, 574
BsaBI GATNNNNATC 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 273, 564, 578, 690
BsaXI ACNNNNNCTCC 2 cut(s) 671, 701
Bsc4I CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
Bse8I GATNNNNATC 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 273, 564, 578, 690
BseGI GGATG 2 cut(s) 789, 1036
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
BseMII CTCAG 4 cut(s) 208, 572, 764, 783
BsePI GCGCGC 1 cut(s) 372
BseRI GAGGAG 6 cut(s) 655, 740, 770, 773, 875, 878
BseSI GKGCMC 2 cut(s) 492, 593
BseXI GCAGC 1 cut(s) 1046
BseYI CCCAGC 1 cut(s) 1039
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 5 cut(s) 278, 455, 563, 569, 577
BshNI GGYRCC 2 cut(s) 266, 472
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 3 cut(s) 246, 273, 573
BsiWI CGTACG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
BsmAI GTCTC 3 cut(s) 343, 561, 1100
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 2 cut(s) 4, 859
BsnI GGCC 5 cut(s) 278, 455, 563, 569, 577
Bsp1286I GDGCHC 3 cut(s) 244, 492, 593
Bsp143I GATC 2 cut(s) 397, 1121
BspACI CCGC 8 cut(s) 188, 249, 255, 264, 648, 857, 1007, 1025
BspANI GGCC 5 cut(s) 278, 455, 563, 569, 577
BspCNI CTCAG 4 cut(s) 207, 571, 765, 784
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
BspPI GGATC 2 cut(s) 392, 405
BspT107I GGYRCC 2 cut(s) 266, 472
BssECI CCNNGG 4 cut(s) 273, 564, 578, 690
BssHII GCGCGC 1 cut(s) 372
BssMI GATC 2 cut(s) 397, 1121
BssNI GRCGYC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 267
BstC8I GCNNGC 3 cut(s) 374, 650, 697
BstDEI CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1053
BstDSI CCRYGG 1 cut(s) 564
BstF5I GGATG 2 cut(s) 789, 1036
BstFNI CGCG 1 cut(s) 374
BstH2I RGCGCY 2 cut(s) 270, 773
BstHHI GCGC 4 cut(s) 269, 374, 376, 772
BstKTI GATC 2 cut(s) 400, 1124
BstMAI GTCTC 3 cut(s) 343, 561, 1100
BstMBI GATC 2 cut(s) 397, 1121
BstMWI GCNNNNNNNGC 7 cut(s) 10, 255, 261, 264, 275, 1031, 1040
BstNSI RCATGY 2 cut(s) 307, 656
BstSCI CCNGG 3 cut(s) 244, 272, 572
BstSLI GKGCMC 2 cut(s) 492, 593
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 1046
BstV2I GAAGAC 1 cut(s) 833
BstX2I RGATCY 2 cut(s) 397, 1121
BstYI RGATCY 2 cut(s) 397, 1121
BsuRI GGCC 5 cut(s) 278, 455, 563, 569, 577
BtgI CCRYGG 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 789, 1036
BtsIMutI CAGTG 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 374, 650, 697
CfoI GCGC 4 cut(s) 269, 374, 376, 772
Cfr13I GGNCC 3 cut(s) 276, 575, 1001
Csp6I GTAC 3 cut(s) 307, 340, 894
CviAII CATG 5 cut(s) 304, 511, 653, 710, 902
CviQI GTAC 3 cut(s) 307, 340, 894
DdeI CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1053
DinI GGCGCC 1 cut(s) 268
DpnI GATC 2 cut(s) 399, 1123
DpnII GATC 2 cut(s) 397, 1121
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 242
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 1 cut(s) 1001
Eco53kI GAGCTC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
FaeI CATG 5 cut(s) 307, 514, 656, 713, 905
FaiI YATR 7 cut(s) 305, 512, 654, 711, 903, 1065, 1111
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 303, 510, 652, 709, 901
FauI CCCGC 2 cut(s) 195, 641
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 256, 265, 1035
FokI GGATG 2 cut(s) 796, 1043
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 3 cut(s) 256, 265, 1035
GlaI GCGC 4 cut(s) 268, 373, 375, 771
GluI GCNGC 3 cut(s) 256, 265, 1035
GsaI CCCAGC 1 cut(s) 1043
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 2 cut(s) 270, 773
HaeIII GGCC 5 cut(s) 278, 455, 563, 569, 577
HapII CCGG 3 cut(s) 246, 273, 573
HhaI GCGC 4 cut(s) 269, 374, 376, 772
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 307, 514, 656, 713, 905
Hin6I GCGC 4 cut(s) 267, 372, 374, 770
HinP1I GCGC 4 cut(s) 267, 372, 374, 770
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 2 cut(s) 113, 480
HinfI GANTC 8 cut(s) 38, 179, 220, 281, 351, 548, 554, 788
HpaII CCGG 3 cut(s) 246, 273, 573
HphI GGTGA 4 cut(s) 145, 694, 832, 1021
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 6 cut(s) 202, 239, 366, 553, 724, 793
Hpy8I GTNNAC 1 cut(s) 334
Hpy99I CGWCG 1 cut(s) 1087
HpyCH4V TGCA 5 cut(s) 4, 232, 523, 665, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 255, 261, 264, 275, 1031, 1040
HpyF3I CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1053
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 307, 514, 656, 713, 905
HspAI GCGC 4 cut(s) 267, 372, 374, 770
KasI GGCGCC 1 cut(s) 266
Kzo9I GATC 2 cut(s) 397, 1121
LmnI GCTCC 2 cut(s) 247, 439
Lsp1109I GCAGC 1 cut(s) 1046
LweI GCATC 1 cut(s) 1021
MalI GATC 2 cut(s) 399, 1123
MboI GATC 2 cut(s) 397, 1121
MboII GAAGA 7 cut(s) 22, 478, 826, 835, 838, 1024, 1105
MflI RGATCY 2 cut(s) 397, 1121
MhlI GDGCHC 3 cut(s) 244, 492, 593
MluCI AATT 4 cut(s) 84, 204, 225, 233
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 6 cut(s) 173, 214, 290, 345, 563, 797
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 428, 482
MseI TTAA 6 cut(s) 495, 503, 537, 612, 1020, 1101
MslI CAYNNNNRTG 1 cut(s) 911
MspI CCGG 3 cut(s) 246, 273, 573
MspR9I CCNGG 3 cut(s) 246, 274, 574
Mva1269I GAATGC 2 cut(s) 4, 859
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 7 cut(s) 10, 255, 261, 264, 275, 1031, 1040
NarI GGCGCC 1 cut(s) 267
NciI CCSGG 3 cut(s) 246, 274, 574
NdeII GATC 2 cut(s) 397, 1121
NlaIII CATG 5 cut(s) 307, 514, 656, 713, 905
NlaIV GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
NmeAIII GCCGAG 4 cut(s) 304, 337, 603, 671
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 307, 656
PauI GCGCGC 1 cut(s) 372
PceI AGGCCT 1 cut(s) 455
PciI ACATGT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 2 cut(s) 4, 859
PdmI GAANNNNTTC 2 cut(s) 428, 482
PfeI GAWTC 2 cut(s) 38, 548
Pfl23II CGTACG 1 cut(s) 339
PkrI GCNGC 3 cut(s) 257, 266, 1036
PleI GAGTC 6 cut(s) 173, 214, 289, 345, 562, 796
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 6 cut(s) 173, 214, 289, 345, 562, 796
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 1039
PspLI CGTACG 1 cut(s) 339
PspN4I GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
PspPI GGNCC 3 cut(s) 276, 575, 1001
PsuI RGATCY 2 cut(s) 397, 1121
PteI GCGCGC 1 cut(s) 372
RsaI GTAC 3 cut(s) 308, 341, 895
RsaNI GTAC 3 cut(s) 307, 340, 894
RseI CAYNNNNRTG 1 cut(s) 911
SacI GAGCTC 1 cut(s) 244
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 6 cut(s) 495, 503, 537, 612, 1020, 1101
SatI GCNGC 3 cut(s) 256, 265, 1035
Sau3AI GATC 2 cut(s) 397, 1121
Sau96I GGNCC 3 cut(s) 276, 575, 1001
SchI GAGTC 6 cut(s) 173, 214, 290, 345, 563, 797
ScrFI CCNGG 3 cut(s) 246, 274, 574
SduI GDGCHC 3 cut(s) 244, 492, 593
SfaNI GCATC 1 cut(s) 1021
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 4 cut(s) 84, 204, 225, 233
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 8 cut(s) 188, 249, 255, 264, 648, 857, 1007, 1025
SspDI GGCGCC 1 cut(s) 266
SstI GAGCTC 1 cut(s) 244
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 244, 272, 572
TasI AATT 4 cut(s) 84, 204, 225, 233
TauI GCSGC 2 cut(s) 258, 267
TfiI GAWTC 2 cut(s) 38, 548
Tru1I TTAA 6 cut(s) 495, 503, 537, 612, 1020, 1101
Tru9I TTAA 6 cut(s) 495, 503, 537, 612, 1020, 1101
TscAI CASTG 1 cut(s) 593
TseI GCWGC 1 cut(s) 1034
TspRI CASTG 1 cut(s) 593
VpaK11BI GGWCC 1 cut(s) 1001
XapI RAATTY 1 cut(s) 204
XceI RCATGY 2 cut(s) 307, 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 428, 482
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.