Rroxscaffold_2G00154910

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014164
Physical Location & Seq
Reverse (-)
91358169 .. 91360874
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154910.1

Sequence Viewer

Length: 1137 bp
ATGCATTCCAAGCCCCAAACTTTCCAAATCTTCCTCGAATCCCCACAACTCGAAATCCCAACCCAAACCCTAACCCTATCCCCAATTCAAACCCCAACTCTCCACCACCTCAAGCTTTCTCTTTTCTCCCAAACCCTTCCCTCCCTCTACTTCACCCTCAATGGAAAGCCCTTACTCGACTCCACCCCGCTTCTCAGTTCCGAAATTTCCCCCCTTTCGACCCTAATTTTGCAATTCAGAGCTCCCGGCGGAGGCGGCGATGGCGGCGCCACCGGGGCCGAGTCGAGGGATTGCTATCTCAACATGTACGCCGAGAAAAAGCCTGATAAGGTCGACCCGTACGAGCAGAGACTCTCCAAGTTTCTGAACTGCGCGCTTTCCAATGAGCCATTGAAGGATCCTTGCGTTGTGGATTTGCTTGGGAATGTGTTCAACAAGGAGCCTCTAGTCGAGGCCTTGTTGGGGAAGAAGGTGCCGAAAGCTTTCGGGCACATTAAGGGTTTAAGGGACATGGTTCCTGTGCATTTTACTTCGATTAACTTGCCCGAATCGGAGTCTCAGGCCACGGCCACCGGACCGAGGTTTCAGTGCCCCATTACGGGGCTGGAGTTTAATGGGAAGTCTAAGTTTCTGGCGTTGAGGAGTTGCGGGCATGTGTTGAGTGCAAAGGCATTGAAGGAGGTGAAGTCCTCGGCTTGCCTTGTGTGTCATGTGGGGTTTTCGGAGGAGGATAAAATTGTGATAAATGGGAATGAGGAGGAGGTCGCGGCGCTGAGGGAGAGGATGGAGGCTGAGAAGGCGAAATCGAGGGGAAAGAAGGTGAAGAAGACGAAAAATGGGGAGGGGGTTGGGAATGCGGAGGAGGAGGGTTCGAGGTTGAGTGGTACGAAGCATGGCATTGATGTGAAGGGTGTGGAGAAGGCTTCGGCGAAGGTGGAGGGGAATGGGAAGGTTGGGAATGGTGGGGAGAATGTGAAGGGTGTGAGGAATGGTCCTGTGGTGAAGAAGTTTAAGGCGGTGGATGCAGCCCCAGCTAATGCTACTAAGGAAGTGTATGCTTCGATTTTCACTTCGTCAAGCAAGAAGAAGTTTAAGGAGACATATTCTTGTAGATCTCTCCCACTTGGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

378

Amino Acids

40.81

Weight (kDa)

9.19

Isoelectric Point (pI)

37.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 84 - 363 3.7e-74 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 266, 472
AccI GTMKAC 1 cut(s) 333
AccII CGCG 2 cut(s) 374, 767
AciI CCGC 8 cut(s) 188, 249, 255, 264, 648, 767, 857, 1016
AclWI GGATC 2 cut(s) 392, 405
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 204
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 3 cut(s) 308, 341, 886
AfiI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 4 cut(s) 89, 394, 433, 676
AluBI AGCT 4 cut(s) 115, 242, 482, 1034
AluI AGCT 4 cut(s) 115, 242, 482, 1034
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 343, 561, 1091
AlwI GGATC 2 cut(s) 392, 405
AoxI GGCC 4 cut(s) 276, 453, 561, 567
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 1 cut(s) 204
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
Asp700I GAANNNNTTC 2 cut(s) 428, 482
AspLEI GCGC 4 cut(s) 269, 374, 376, 772
AspS9I GGNCC 3 cut(s) 276, 575, 992
AsuC2I CCSGG 2 cut(s) 246, 274
AsuHPI GGTGA 4 cut(s) 145, 694, 832, 1012
AvaII GGWCC 2 cut(s) 575, 992
BaeGI GKGCMC 2 cut(s) 492, 593
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 2 cut(s) 266, 472
BanII GRGCYC 1 cut(s) 244
BbsI GAAGAC 1 cut(s) 833
Bbv12I GWGCWC 1 cut(s) 244
BbvCI CCTCAGC 1 cut(s) 773
BbvI GCAGC 1 cut(s) 1037
BccI CCATC 2 cut(s) 254, 778
BceAI ACGGC 1 cut(s) 582
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BcnI CCSGG 2 cut(s) 246, 274
BcoDI GTCTC 3 cut(s) 343, 561, 1091
BfaI CTAG 1 cut(s) 446
BfoI RGCGCY 2 cut(s) 270, 773
BglI GCCNNNNNGGC 1 cut(s) 275
BglII AGATCT 1 cut(s) 1112
BisI GCNGC 4 cut(s) 256, 265, 768, 1026
BlsI GCNGC 4 cut(s) 257, 266, 769, 1027
Bme1390I CCNGG 2 cut(s) 246, 274
Bme18I GGWCC 2 cut(s) 575, 992
BmgT120I GGNCC 3 cut(s) 276, 575, 992
BmiI GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
BmrFI CCNGG 2 cut(s) 246, 274
BmsI GCATC 1 cut(s) 1012
BpiI GAAGAC 1 cut(s) 833
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 246, 274
BsaBI GATNNNNATC 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 273, 564, 578, 690
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 671, 701
Bsc4I CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
Bse8I GATNNNNATC 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 273, 564, 578, 690
BseGI GGATG 2 cut(s) 789, 1027
BseJI GATNNNNATC 1 cut(s) 294
BseLI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
BseMII CTCAG 4 cut(s) 208, 572, 764, 783
BsePI GCGCGC 1 cut(s) 372
BseRI GAGGAG 6 cut(s) 655, 740, 770, 773, 875, 878
BseSI GKGCMC 2 cut(s) 492, 593
BseXI GCAGC 1 cut(s) 1037
BseYI CCCAGC 1 cut(s) 1030
Bsh1236I CGCG 2 cut(s) 374, 767
BshFI GGCC 4 cut(s) 278, 455, 563, 569
BshNI GGYRCC 2 cut(s) 266, 472
BsiHKAI GWGCWC 1 cut(s) 244
BsiSI CCGG 3 cut(s) 246, 273, 573
BsiWI CGTACG 1 cut(s) 339
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 7 cut(s) 251, 285, 462, 579, 598, 599, 600
BsmAI GTCTC 3 cut(s) 343, 561, 1091
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 2 cut(s) 4, 859
BsnI GGCC 4 cut(s) 278, 455, 563, 569
Bsp1286I GDGCHC 3 cut(s) 244, 492, 593
Bsp143I GATC 2 cut(s) 397, 1112
BspACI CCGC 8 cut(s) 188, 249, 255, 264, 648, 767, 857, 1016
BspANI GGCC 4 cut(s) 278, 455, 563, 569
BspCNI CTCAG 4 cut(s) 207, 571, 765, 784
BspFNI CGCG 2 cut(s) 374, 767
BspLI GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
BspPI GGATC 2 cut(s) 392, 405
BspT107I GGYRCC 2 cut(s) 266, 472
BssECI CCNNGG 4 cut(s) 273, 564, 578, 690
BssHII GCGCGC 1 cut(s) 372
BssMI GATC 2 cut(s) 397, 1112
BssNI GRCGYC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 267
BstC8I GCNNGC 3 cut(s) 374, 650, 697
BstDEI CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1044
BstDSI CCRYGG 1 cut(s) 564
BstF5I GGATG 2 cut(s) 789, 1027
BstFNI CGCG 2 cut(s) 374, 767
BstH2I RGCGCY 2 cut(s) 270, 773
BstHHI GCGC 4 cut(s) 269, 374, 376, 772
BstKTI GATC 2 cut(s) 400, 1115
BstMAI GTCTC 3 cut(s) 343, 561, 1091
BstMBI GATC 2 cut(s) 397, 1112
BstMWI GCNNNNNNNGC 8 cut(s) 10, 255, 261, 264, 275, 797, 1022, 1031
BstNSI RCATGY 2 cut(s) 307, 656
BstSCI CCNGG 2 cut(s) 244, 272
BstSLI GKGCMC 2 cut(s) 492, 593
BstUI CGCG 2 cut(s) 374, 767
BstV1I GCAGC 1 cut(s) 1037
BstV2I GAAGAC 1 cut(s) 833
BstX2I RGATCY 2 cut(s) 397, 1112
BstYI RGATCY 2 cut(s) 397, 1112
BsuRI GGCC 4 cut(s) 278, 455, 563, 569
BtgI CCRYGG 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 789, 1027
BtsIMutI CAGTG 1 cut(s) 593
Cac8I GCNNGC 3 cut(s) 374, 650, 697
CfoI GCGC 4 cut(s) 269, 374, 376, 772
Cfr13I GGNCC 3 cut(s) 276, 575, 992
CpoI CGGWCCG 1 cut(s) 575
Csp6I GTAC 3 cut(s) 307, 340, 885
CspI CGGWCCG 1 cut(s) 575
CviAII CATG 5 cut(s) 304, 511, 653, 710, 893
CviQI GTAC 3 cut(s) 307, 340, 885
DdeI CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1044
DinI GGCGCC 1 cut(s) 268
DpnI GATC 2 cut(s) 399, 1114
DpnII GATC 2 cut(s) 397, 1112
EaeI YGGCCR 1 cut(s) 567
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 242
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 575, 992
Eco53kI GAGCTC 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 244
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
FaeI CATG 5 cut(s) 307, 514, 656, 713, 896
FaiI YATR 7 cut(s) 305, 512, 654, 711, 894, 1056, 1102
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 303, 510, 652, 709, 892
FauI CCCGC 2 cut(s) 195, 641
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 4 cut(s) 256, 265, 768, 1026
FokI GGATG 2 cut(s) 796, 1034
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 4 cut(s) 256, 265, 768, 1026
FspBI CTAG 1 cut(s) 446
GlaI GCGC 4 cut(s) 268, 373, 375, 771
GluI GCNGC 4 cut(s) 256, 265, 768, 1026
GsaI CCCAGC 1 cut(s) 1034
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 2 cut(s) 270, 773
HaeIII GGCC 4 cut(s) 278, 455, 563, 569
HapII CCGG 3 cut(s) 246, 273, 573
HhaI GCGC 4 cut(s) 269, 374, 376, 772
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 307, 514, 656, 713, 896
Hin6I GCGC 4 cut(s) 267, 372, 374, 770
HinP1I GCGC 4 cut(s) 267, 372, 374, 770
HincII GTYRAC 1 cut(s) 334
HindII GTYRAC 1 cut(s) 334
HindIII AAGCTT 2 cut(s) 113, 480
HinfI GANTC 6 cut(s) 38, 179, 281, 351, 548, 554
HpaII CCGG 3 cut(s) 246, 273, 573
HphI GGTGA 4 cut(s) 145, 694, 832, 1012
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 5 cut(s) 202, 239, 366, 553, 724
Hpy8I GTNNAC 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 4, 232, 523, 665, 1025
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 255, 261, 264, 275, 797, 1022, 1031
HpyF3I CTNAG 6 cut(s) 194, 558, 624, 773, 792, 1044
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 307, 514, 656, 713, 896
HspAI GCGC 4 cut(s) 267, 372, 374, 770
KasI GGCGCC 1 cut(s) 266
Kzo9I GATC 2 cut(s) 397, 1112
LmnI GCTCC 2 cut(s) 247, 439
Lsp1109I GCAGC 1 cut(s) 1037
LweI GCATC 1 cut(s) 1012
MaeI CTAG 1 cut(s) 446
MalI GATC 2 cut(s) 399, 1114
MboI GATC 2 cut(s) 397, 1112
MboII GAAGA 6 cut(s) 22, 478, 835, 838, 1015, 1096
MflI RGATCY 2 cut(s) 397, 1112
MhlI GDGCHC 3 cut(s) 244, 492, 593
MluCI AATT 5 cut(s) 84, 204, 225, 233, 735
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 4 cut(s) 173, 290, 345, 563
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 428, 482
MseI TTAA 6 cut(s) 495, 503, 537, 612, 1011, 1092
MslI CAYNNNNRTG 1 cut(s) 902
MspI CCGG 3 cut(s) 246, 273, 573
MspR9I CCNGG 2 cut(s) 246, 274
Mva1269I GAATGC 2 cut(s) 4, 859
MvnI CGCG 2 cut(s) 374, 767
MwoI GCNNNNNNNGC 8 cut(s) 10, 255, 261, 264, 275, 797, 1022, 1031
NarI GGCGCC 1 cut(s) 267
NciI CCSGG 2 cut(s) 246, 274
NdeII GATC 2 cut(s) 397, 1112
NlaIII CATG 5 cut(s) 307, 514, 656, 713, 896
NlaIV GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
NmeAIII GCCGAG 3 cut(s) 304, 337, 671
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 307, 656
PauI GCGCGC 1 cut(s) 372
PceI AGGCCT 1 cut(s) 455
PciI ACATGT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 2 cut(s) 4, 859
PdmI GAANNNNTTC 2 cut(s) 428, 482
PfeI GAWTC 2 cut(s) 38, 548
Pfl23II CGTACG 1 cut(s) 339
PkrI GCNGC 4 cut(s) 257, 266, 769, 1027
PleI GAGTC 4 cut(s) 173, 289, 345, 562
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 4 cut(s) 173, 289, 345, 562
PscI ACATGT 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 244
PspFI CCCAGC 1 cut(s) 1030
PspLI CGTACG 1 cut(s) 339
PspN4I GGNNCC 6 cut(s) 268, 277, 399, 441, 474, 516
PspPI GGNCC 3 cut(s) 276, 575, 992
PsuI RGATCY 2 cut(s) 397, 1112
PteI GCGCGC 1 cut(s) 372
RsaI GTAC 3 cut(s) 308, 341, 886
RsaNI GTAC 3 cut(s) 307, 340, 885
RseI CAYNNNNRTG 1 cut(s) 902
Rsr2I CGGWCCG 1 cut(s) 575
RsrII CGGWCCG 1 cut(s) 575
SacI GAGCTC 1 cut(s) 244
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 6 cut(s) 495, 503, 537, 612, 1011, 1092
SatI GCNGC 4 cut(s) 256, 265, 768, 1026
Sau3AI GATC 2 cut(s) 397, 1112
Sau96I GGNCC 3 cut(s) 276, 575, 992
SchI GAGTC 4 cut(s) 173, 290, 345, 563
ScrFI CCNGG 2 cut(s) 246, 274
SduI GDGCHC 3 cut(s) 244, 492, 593
SfaNI GCATC 1 cut(s) 1012
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 2 cut(s) 575, 992
SmiMI CAYNNNNRTG 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 5 cut(s) 84, 204, 225, 233, 735
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 8 cut(s) 188, 249, 255, 264, 648, 767, 857, 1016
SspDI GGCGCC 1 cut(s) 266
SspMI CTAG 1 cut(s) 446
SstI GAGCTC 1 cut(s) 244
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 2 cut(s) 244, 272
TaqII GACCGA 1 cut(s) 592
TasI AATT 5 cut(s) 84, 204, 225, 233, 735
TauI GCSGC 3 cut(s) 258, 267, 770
TfiI GAWTC 2 cut(s) 38, 548
Tru1I TTAA 6 cut(s) 495, 503, 537, 612, 1011, 1092
Tru9I TTAA 6 cut(s) 495, 503, 537, 612, 1011, 1092
TscAI CASTG 1 cut(s) 593
TseI GCWGC 1 cut(s) 1025
TspRI CASTG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 575, 992
XapI RAATTY 1 cut(s) 204
XceI RCATGY 2 cut(s) 307, 656
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 428, 482
XspI CTAG 1 cut(s) 446
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.