MD15G1155100.v1.1

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014164
Physical Location & Seq
Forward (+)
11501915 .. 11503831
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1155100.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGCATCCCAAACTTCAAAATTTCCAAATCTTCCTGCAATCTCCCGATCTCCAAATCCCAATTCAAACCCTAACCCTATCCCCAATTCAGACCCCAACTCTGCGGCACCTCAAGCTCTCAGTTTTCTCCCATGTCCTACCATCGCTTTACTTCACCCTCAATGGTAAGCCCCTAAACGATTCCACTTCGCTTTTCGATTCCCAAATTACCCCTCTTTCTACTCTGATTTTACGAATTAGAGCGCTTGGCGGTGGCGGCGATGGCGGCGCGACAGGAGCGGAGTCTCGCGACTGCTATCTCAAGATGTACGCGGAGAAGAAGCCCGATAAGGTCGACCCGAACGAGCAGAGGCTTTCGAAGTGGCTCAATTGTGCGCTGTCGAACGAGCCATTGAAGGAGCCGTGCGTGATGGATTTTCTGGGGAACGTGTTTAACAAGGAAGCTCTTGTGGAGGCGTTGTTGGGGAAGAAGGTGCCCAAGGCGTTTGGGCATATAAAGGGGTTGAAGGATATGATAGGTATTCATCTTACCCCAATCACCGGGGCTGAATTCAATCGCCAATCCGTTGCCGGGCCACGGTTTCAGTGTCCCATTACAGGGGTCGAGTTCAACGGTAAGTGTAAGTTTGTGGGATTAAGAACTTGTGGGCATGTGTTGAGTTCAAAGGCTTTGAAGGAGATAAAGTCGTCAACTTGCCTTGTTTGCCATGCGGGATTTTCGGAGGCGGATAAGATTGTGATTAATGGGAATGAAGAGGAAATTGCGGAGTTGAGGGAGAGGATGGAGGCCGAGAAGGTGAAAGGGAGAGTGAAGAAGGTGAAGAAGACGAAAAACTGGAATGCTGGTGTGAATGGCGAGGAGGGTGTGGATTTGGAGGCCTCACGGTTGAGTGGTACCAAGCATGGTATTGATGCTAGGGCTGTGGAGAAGGCTTCAACAAAGTTAGAGGTAAATGTGAAGGTTGTGAATGGTGGGGCTGATGTGAAGGGTGCGAATAATGGTGGGGCGAAGAGGTTCAAGGCGGTGGATATTGCCCCTGCTAATGCTACTAAGGAACTGTATGCGTCCATCTTCACGTCATCAAGGAAGAAGGATTTCAAGGAAACATTTTCTTGCAGATCTCTCCCACTTGGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

381

Amino Acids

41.46

Weight (kDa)

9.39

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 84 - 364 4.3e-74 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 893
AccB1I GGYRCC 3 cut(s) 105, 472, 893
AccBSI CCGCTC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 333
AccII CGCG 3 cut(s) 269, 288, 311
AcsI RAATTY 2 cut(s) 19, 548
AfaI GTAC 2 cut(s) 308, 895
AfeI AGCGCT 1 cut(s) 243
AfiI CCNNNNNNNGG 5 cut(s) 539, 570, 576, 596, 597
AflIII ACRYGT 1 cut(s) 426
AjiI CACGTC 1 cut(s) 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AluBI AGCT 2 cut(s) 115, 443
AluI AGCT 2 cut(s) 115, 443
Alw26I GTCTC 1 cut(s) 288
Aor51HI AGCGCT 1 cut(s) 243
AoxI GGCC 3 cut(s) 572, 786, 876
ApoI RAATTY 2 cut(s) 19, 548
AseI ATTAAT 1 cut(s) 741
Asp700I GAANNNNTTC 2 cut(s) 1013, 1094
Asp718I GGTACC 1 cut(s) 893
AspLEI GCGC 3 cut(s) 244, 269, 376
AspS9I GGNCC 1 cut(s) 572
AsuC2I CCSGG 2 cut(s) 541, 571
AsuHPI GGTGA 4 cut(s) 145, 529, 808, 829
AsuII TTCGAA 1 cut(s) 356
BaeGI GKGCMC 1 cut(s) 477
BanI GGYRCC 3 cut(s) 105, 472, 893
BbsI GAAGAC 1 cut(s) 830
BccI CCATC 5 cut(s) 148, 254, 403, 775, 1076
BceAI ACGGC 1 cut(s) 385
BcnI CCSGG 2 cut(s) 541, 571
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 245
BglII AGATCT 1 cut(s) 1118
BisI GCNGC 3 cut(s) 104, 256, 265
BlsI GCNGC 3 cut(s) 105, 257, 266
Bme1390I CCNGG 2 cut(s) 541, 571
BmgBI CACGTC 1 cut(s) 1077
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 4 cut(s) 107, 399, 474, 895
BmrFI CCNGG 2 cut(s) 541, 571
BmsI GCATC 2 cut(s) 13, 901
BpiI GAAGAC 1 cut(s) 830
Bpu14I TTCGAA 1 cut(s) 356
BpuEI CTTGAG 2 cut(s) 95, 284
BpuMI CCSGG 2 cut(s) 541, 571
BsaJI CCNNGG 3 cut(s) 477, 540, 575
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 668, 698
Bsc4I CCNNNNNNNGG 5 cut(s) 539, 570, 576, 596, 597
Bse1I ACTGG 1 cut(s) 839
BseDI CCNNGG 3 cut(s) 477, 540, 575
BseGI GGATG 2 cut(s) 4, 786
BseLI CCNNNNNNNGG 5 cut(s) 539, 570, 576, 596, 597
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 1 cut(s) 839
BseRI GAGGAG 1 cut(s) 872
BseSI GKGCMC 1 cut(s) 477
Bsh1236I CGCG 3 cut(s) 269, 288, 311
BshFI GGCC 3 cut(s) 574, 788, 878
BshNI GGYRCC 3 cut(s) 105, 472, 893
BsiSI CCGG 2 cut(s) 540, 570
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 5 cut(s) 539, 570, 576, 596, 597
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 3 cut(s) 574, 788, 878
Bsp119I TTCGAA 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 2 cut(s) 46, 1118
Bsp68I TCGCGA 1 cut(s) 288
BspANI GGCC 3 cut(s) 574, 788, 878
BspCNI CTCAG 1 cut(s) 131
BspFNI CGCG 3 cut(s) 269, 288, 311
BspLI GGNNCC 4 cut(s) 107, 399, 474, 895
BspT104I TTCGAA 1 cut(s) 356
BspT107I GGYRCC 3 cut(s) 105, 472, 893
BsrBI CCGCTC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 839
BssECI CCNNGG 3 cut(s) 477, 540, 575
BssMI GATC 2 cut(s) 46, 1118
BssT1I CCWWGG 1 cut(s) 477
Bst4CI ACNGT 4 cut(s) 579, 614, 885, 1059
Bst6I CTCTTC 2 cut(s) 747, 1004
BstBI TTCGAA 1 cut(s) 356
BstDEI CTNAG 2 cut(s) 118, 1050
BstDSI CCRYGG 1 cut(s) 575
BstF5I GGATG 2 cut(s) 4, 786
BstFNI CGCG 3 cut(s) 269, 288, 311
BstH2I RGCGCY 1 cut(s) 245
BstHHI GCGC 3 cut(s) 244, 269, 376
BstKTI GATC 2 cut(s) 49, 1121
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 2 cut(s) 46, 1118
BstMWI GCNNNNNNNGC 6 cut(s) 112, 255, 261, 264, 275, 702
BstNSI RCATGY 1 cut(s) 653
BstSCI CCNGG 2 cut(s) 539, 569
BstSLI GKGCMC 1 cut(s) 477
BstUI CGCG 3 cut(s) 269, 288, 311
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 1 cut(s) 1118
BstYI RGATCY 1 cut(s) 1118
BsuRI GGCC 3 cut(s) 574, 788, 878
BtgI CCRYGG 1 cut(s) 575
BtgZI GCGATG 2 cut(s) 126, 273
BtrI CACGTC 1 cut(s) 1077
BtsCI GGATG 2 cut(s) 4, 786
BtsIMutI CAGTG 1 cut(s) 590
BtuMI TCGCGA 1 cut(s) 288
CfoI GCGC 3 cut(s) 244, 269, 376
Cfr13I GGNCC 1 cut(s) 572
CseI GACGC 1 cut(s) 1053
Csp6I GTAC 2 cut(s) 307, 894
CviAII CATG 4 cut(s) 131, 650, 707, 902
CviQI GTAC 2 cut(s) 307, 894
DdeI CTNAG 2 cut(s) 118, 1050
DpnI GATC 2 cut(s) 48, 1120
DpnII GATC 2 cut(s) 46, 1118
Eam1104I CTCTTC 2 cut(s) 747, 1004
EarI CTCTTC 2 cut(s) 747, 1004
EciI GGCGGA 1 cut(s) 740
Eco130I CCWWGG 1 cut(s) 477
Eco147I AGGCCT 1 cut(s) 878
Eco47III AGCGCT 1 cut(s) 243
EcoRI GAATTC 1 cut(s) 548
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 4 cut(s) 134, 653, 710, 905
FaiI YATR 8 cut(s) 132, 492, 494, 512, 651, 708, 903, 1062
FaqI GGGAC 1 cut(s) 573
FatI CATG 4 cut(s) 130, 649, 706, 901
FauI CCCGC 1 cut(s) 703
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 3 cut(s) 104, 256, 265
FokI GGATG 1 cut(s) 793
Fsp4HI GCNGC 3 cut(s) 104, 256, 265
FspBI CTAG 1 cut(s) 915
GlaI GCGC 3 cut(s) 243, 268, 375
GluI GCNGC 3 cut(s) 104, 256, 265
HaeII RGCGCY 1 cut(s) 245
HaeIII GGCC 3 cut(s) 574, 788, 878
HapII CCGG 2 cut(s) 540, 570
HgaI GACGC 1 cut(s) 1053
HhaI GCGC 3 cut(s) 244, 269, 376
Hin1II CATG 4 cut(s) 134, 653, 710, 905
Hin6I GCGC 3 cut(s) 242, 267, 374
HinP1I GCGC 3 cut(s) 242, 267, 374
HincII GTYRAC 2 cut(s) 334, 690
HindII GTYRAC 2 cut(s) 334, 690
HinfI GANTC 3 cut(s) 179, 197, 281
HpaII CCGG 2 cut(s) 540, 570
HphI GGTGA 4 cut(s) 145, 529, 808, 829
Hpy166II GTNNAC 2 cut(s) 334, 690
Hpy188I TCNGA 3 cut(s) 90, 225, 721
Hpy188III TCNNGA 3 cut(s) 44, 287, 301
Hpy8I GTNNAC 2 cut(s) 334, 690
HpyCH4III ACNGT 4 cut(s) 579, 614, 885, 1059
HpyCH4IV ACGT 2 cut(s) 426, 1076
HpyCH4V TGCA 3 cut(s) 4, 37, 1116
HpyF10VI GCNNNNNNNGC 6 cut(s) 112, 255, 261, 264, 275, 702
HpyF3I CTNAG 2 cut(s) 118, 1050
HpySE526I ACGT 2 cut(s) 426, 1076
Hsp92II CATG 4 cut(s) 134, 653, 710, 905
HspAI GCGC 3 cut(s) 242, 267, 374
KpnI GGTACC 1 cut(s) 897
Kzo9I GATC 2 cut(s) 46, 1118
LmnI GCTCC 2 cut(s) 275, 397
LpnPI CCDG 9 cut(s) 47, 258, 404, 553, 582, 583, 820, 828, 1050
LweI GCATC 2 cut(s) 13, 901
MaeI CTAG 1 cut(s) 915
MaeII ACGT 2 cut(s) 426, 1076
MalI GATC 2 cut(s) 48, 1120
MbiI CCGCTC 1 cut(s) 278
MboI GATC 2 cut(s) 46, 1118
MfeI CAATTG 1 cut(s) 367
MflI RGATCY 1 cut(s) 1118
MhlI GDGCHC 1 cut(s) 477
MluCI AATT 8 cut(s) 19, 60, 84, 204, 234, 367, 548, 759
MlyI GAGTC 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 1013, 1094
MseI TTAA 3 cut(s) 432, 635, 741
MspI CCGG 2 cut(s) 540, 570
MspR9I CCNGG 2 cut(s) 541, 571
MunI CAATTG 1 cut(s) 367
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 3 cut(s) 269, 288, 311
MwoI GCNNNNNNNGC 6 cut(s) 112, 255, 261, 264, 275, 702
NciI CCSGG 2 cut(s) 541, 571
NdeII GATC 2 cut(s) 46, 1118
NlaIII CATG 4 cut(s) 134, 653, 710, 905
NlaIV GGNNCC 4 cut(s) 107, 399, 474, 895
NmeAIII GCCGAG 1 cut(s) 814
NruI TCGCGA 1 cut(s) 288
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 653
NspV TTCGAA 1 cut(s) 356
PceI AGGCCT 1 cut(s) 878
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 844
PdmI GAANNNNTTC 2 cut(s) 1013, 1094
PfeI GAWTC 2 cut(s) 179, 197
PkrI GCNGC 3 cut(s) 105, 257, 266
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 741
PspN4I GGNNCC 4 cut(s) 107, 399, 474, 895
PspPI GGNCC 1 cut(s) 572
PsuI RGATCY 1 cut(s) 1118
RruI TCGCGA 1 cut(s) 288
RsaI GTAC 2 cut(s) 308, 895
RsaNI GTAC 2 cut(s) 307, 894
SalI GTCGAC 1 cut(s) 332
SaqAI TTAA 3 cut(s) 432, 635, 741
SatI GCNGC 3 cut(s) 104, 256, 265
Sau3AI GATC 2 cut(s) 46, 1118
Sau96I GGNCC 1 cut(s) 572
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 541, 571
SduI GDGCHC 1 cut(s) 477
SfaNI GCATC 2 cut(s) 13, 901
SfuI TTCGAA 1 cut(s) 356
SmlI CTYRAG 2 cut(s) 110, 299
SmoI CTYRAG 2 cut(s) 110, 299
Sse9I AATT 8 cut(s) 19, 60, 84, 204, 234, 367, 548, 759
SseBI AGGCCT 1 cut(s) 878
SspMI CTAG 1 cut(s) 915
StuI AGGCCT 1 cut(s) 878
StyD4I CCNGG 2 cut(s) 539, 569
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 4 cut(s) 579, 614, 885, 1059
TaiI ACGT 2 cut(s) 429, 1079
TaqI TCGA 5 cut(s) 195, 333, 356, 380, 603
TasI AATT 8 cut(s) 19, 60, 84, 204, 234, 367, 548, 759
TauI GCSGC 3 cut(s) 106, 258, 267
TfiI GAWTC 2 cut(s) 179, 197
Tru1I TTAA 3 cut(s) 432, 635, 741
Tru9I TTAA 3 cut(s) 432, 635, 741
TscAI CASTG 1 cut(s) 590
TspDTI ATGAA 2 cut(s) 512, 765
TspGWI ACGGA 1 cut(s) 553
TspRI CASTG 1 cut(s) 590
VspI ATTAAT 1 cut(s) 741
XapI RAATTY 2 cut(s) 19, 548
XceI RCATGY 1 cut(s) 653
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 2 cut(s) 1013, 1094
XspI CTAG 1 cut(s) 915
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.