Rh2DG016000

Protein RTF2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014164
Physical Location & Seq
Forward (+)
927300 .. 928142
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG016000.1

Sequence Viewer

Length: 843 bp
ATGTACGCCGAGAAAAAGCCTGATAAGGTCGACCCGTACGAGCAGAGACTCTCCAAGTTTCTGAACTGCGCGCTTTCCAATGAGCCATTGAAGGATCCTTGCGTTGTGGATTTGCTTGGGAATGTGTTCAATAAGGAGCCTCTGGTTGAGGCCTTGTTGGGGAAGAAGGTGCCGAAAGCTTTCGGGCACATTAAGGGTTTAAGGGACATGGTTCCTGTGCATTTTACTTCGATTAACTTGCCCGAATCGGAGTCTCAGGCCACGGCCACCGGACCGAGGTTTCAGTGCCCCATTACGGGGCTGGAGTTTAATGGGAAGTCTAAGTTTCTGGCGTTGAGGAGTTGCGGGCATGTGTTGAGTGCAAAGGCATTGAAGGAGGTGAAGTCCTCGGCTTGCCTTGTGTGTCATGTGGGGTTTTCTGAGGAGGATAAGATTGTGATAAATGGGAATGAGGAGGAGGTCGCGGCGCTGAGGGAGAGGATGGAGTCTGAGAAGGCGAAATCGAGGGGGAAGAAGGTGAAGAAGACGAAAAATGGGGAGGGGGTTGGGAATGCGGAGGAGGAGGGTGTGGAGGTTTCGAGGTTGAGTGGTACGAAGCATGGCATTGATGTGAAGGGTGTGGAGAAGGCTTCGGCGAAGGTGGAGGGGAATGGAAAGGTTGGGAATGGTGGGGAGAATGTGAAGGGTGTGAGTAATGGTCCTGCGGTCAAGAAGTTTAAGGCGGTGGATGCAGCCCCAGCTAATGCTACTAAGGAAGTGTATGCTTCGATTTTCACTTCGTCGAGCAAGAAGAAGTTTAAGGAGACATATTCTTGTAGATCTCTCCCACTTGGTAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rtf2

Protein Analysis

280

Amino Acids

30.15

Weight (kDa)

9.27

Isoelectric Point (pI)

30.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rtf2 PF04641 4 - 265 9.9e-69 Rtf2 RING-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014164)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58020
fragaria_vesca FvH4_1g01150
malus_domestica MD15G1155100.v1.1
prunus_persica Prupe.7G260500_v2.0.a1
pyrus_communis pycom15g13860
rosa_chinensis RchiOBHm_Chr2g0085941
rosa_laevigata RLG00000015728
rosa_multiflora Rmu_sc0001748.1_g000001 Rmu_ssc0000355.1_g000028
rosa_roxburghii Rroxscaffold_2G00154910
rosa_rugosa Rorug01G0464500 Rorug01G0464500
rosa_samantha Rh2AG013500 Rh2BG014200 Rh2CG014700 Rh2DG016000
rosa_wichuraiana Rw2G001210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 169
AccI GTMKAC 1 cut(s) 30
AccII CGCG 2 cut(s) 71, 464
AciI CCGC 5 cut(s) 345, 464, 554, 704, 722
AclWI GGATC 2 cut(s) 89, 102
AcoI YGGCCR 1 cut(s) 264
AfaI GTAC 3 cut(s) 5, 38, 592
AfiI CCNNNNNNNGG 5 cut(s) 159, 276, 295, 296, 297
AgsI TTSAA 3 cut(s) 91, 130, 373
AluBI AGCT 2 cut(s) 179, 740
AluI AGCT 2 cut(s) 179, 740
Alw26I GTCTC 3 cut(s) 40, 258, 797
AlwI GGATC 2 cut(s) 89, 102
AoxI GGCC 3 cut(s) 150, 258, 264
ApeKI GCWGC 1 cut(s) 731
Asp700I GAANNNNTTC 2 cut(s) 125, 179
AspLEI GCGC 3 cut(s) 71, 73, 469
AspS9I GGNCC 2 cut(s) 272, 698
AsuHPI GGTGA 2 cut(s) 391, 529
AvaII GGWCC 2 cut(s) 272, 698
BaeGI GKGCMC 2 cut(s) 189, 290
BamHI GGATCC 1 cut(s) 94
BanI GGYRCC 1 cut(s) 169
BbsI GAAGAC 1 cut(s) 530
BbvCI CCTCAGC 1 cut(s) 470
BbvI GCAGC 1 cut(s) 743
BccI CCATC 1 cut(s) 475
BceAI ACGGC 1 cut(s) 279
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BcoDI GTCTC 3 cut(s) 40, 258, 797
BfoI RGCGCY 1 cut(s) 470
BglII AGATCT 1 cut(s) 818
BisI GCNGC 2 cut(s) 465, 732
BlsI GCNGC 2 cut(s) 466, 733
Bme18I GGWCC 2 cut(s) 272, 698
BmgT120I GGNCC 2 cut(s) 272, 698
BmiI GGNNCC 4 cut(s) 96, 138, 171, 213
BmsI GCATC 1 cut(s) 718
BpiI GAAGAC 1 cut(s) 530
BpmI CTGGAG 1 cut(s) 323
Bpu10I CCTNAGC 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 261, 275, 387
BsaWI WCCGGW 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 276, 295, 296, 297
BseDI CCNNGG 3 cut(s) 261, 275, 387
BseGI GGATG 2 cut(s) 486, 733
BseLI CCNNNNNNNGG 5 cut(s) 159, 276, 295, 296, 297
BseMII CTCAG 4 cut(s) 269, 411, 461, 480
BsePI GCGCGC 1 cut(s) 69
BseRI GAGGAG 6 cut(s) 352, 437, 467, 470, 572, 575
BseSI GKGCMC 2 cut(s) 189, 290
BseXI GCAGC 1 cut(s) 743
BseYI CCCAGC 1 cut(s) 736
Bsh1236I CGCG 2 cut(s) 71, 464
BshFI GGCC 3 cut(s) 152, 260, 266
BshNI GGYRCC 1 cut(s) 169
BsiSI CCGG 1 cut(s) 270
BsiWI CGTACG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 5 cut(s) 159, 276, 295, 296, 297
BsmAI GTCTC 3 cut(s) 40, 258, 797
BsmFI GGGAC 1 cut(s) 218
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 152, 260, 266
Bsp1286I GDGCHC 2 cut(s) 189, 290
Bsp143I GATC 2 cut(s) 94, 818
BspACI CCGC 5 cut(s) 345, 464, 554, 704, 722
BspANI GGCC 3 cut(s) 152, 260, 266
BspCNI CTCAG 4 cut(s) 268, 412, 462, 481
BspFNI CGCG 2 cut(s) 71, 464
BspLI GGNNCC 4 cut(s) 96, 138, 171, 213
BspPI GGATC 2 cut(s) 89, 102
BspT107I GGYRCC 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 261, 275, 387
BssHII GCGCGC 1 cut(s) 69
BssMI GATC 2 cut(s) 94, 818
BstC8I GCNNGC 3 cut(s) 71, 347, 394
BstDEI CTNAG 6 cut(s) 255, 321, 420, 470, 489, 750
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 486, 733
BstFNI CGCG 2 cut(s) 71, 464
BstH2I RGCGCY 1 cut(s) 470
BstHHI GCGC 3 cut(s) 71, 73, 469
BstKTI GATC 2 cut(s) 97, 821
BstMAI GTCTC 3 cut(s) 40, 258, 797
BstMBI GATC 2 cut(s) 94, 818
BstMWI GCNNNNNNNGC 2 cut(s) 728, 737
BstNSI RCATGY 1 cut(s) 353
BstSLI GKGCMC 2 cut(s) 189, 290
BstUI CGCG 2 cut(s) 71, 464
BstV1I GCAGC 1 cut(s) 743
BstV2I GAAGAC 1 cut(s) 530
BstX2I RGATCY 2 cut(s) 94, 818
BstYI RGATCY 2 cut(s) 94, 818
BsuRI GGCC 3 cut(s) 152, 260, 266
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 2 cut(s) 486, 733
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 3 cut(s) 71, 347, 394
CfoI GCGC 3 cut(s) 71, 73, 469
Cfr13I GGNCC 2 cut(s) 272, 698
CpoI CGGWCCG 1 cut(s) 272
Csp6I GTAC 3 cut(s) 4, 37, 591
CspI CGGWCCG 1 cut(s) 272
CviAII CATG 4 cut(s) 208, 350, 407, 599
CviQI GTAC 3 cut(s) 4, 37, 591
DdeI CTNAG 6 cut(s) 255, 321, 420, 470, 489, 750
DpnI GATC 2 cut(s) 96, 820
DpnII GATC 2 cut(s) 94, 818
EaeI YGGCCR 1 cut(s) 264
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 272, 698
FaeI CATG 4 cut(s) 211, 353, 410, 602
FaiI YATR 6 cut(s) 209, 351, 408, 600, 762, 808
FaqI GGGAC 1 cut(s) 218
FatI CATG 4 cut(s) 207, 349, 406, 598
FauI CCCGC 1 cut(s) 338
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 2 cut(s) 465, 732
FokI GGATG 2 cut(s) 493, 740
Fsp4HI GCNGC 2 cut(s) 465, 732
GlaI GCGC 3 cut(s) 70, 72, 468
GluI GCNGC 2 cut(s) 465, 732
GsaI CCCAGC 1 cut(s) 740
GsuI CTGGAG 1 cut(s) 323
HaeII RGCGCY 1 cut(s) 470
HaeIII GGCC 3 cut(s) 152, 260, 266
HapII CCGG 1 cut(s) 270
HhaI GCGC 3 cut(s) 71, 73, 469
Hin1II CATG 4 cut(s) 211, 353, 410, 602
Hin6I GCGC 3 cut(s) 69, 71, 467
HinP1I GCGC 3 cut(s) 69, 71, 467
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 4 cut(s) 48, 245, 251, 485
HpaII CCGG 1 cut(s) 270
HphI GGTGA 2 cut(s) 391, 529
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 4 cut(s) 63, 250, 421, 490
Hpy188III TCNNGA 1 cut(s) 709
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 1 cut(s) 784
HpyAV CCTTC 9 cut(s) 85, 160, 367, 487, 508, 607, 619, 631, 676
HpyCH4V TGCA 3 cut(s) 220, 362, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 728, 737
HpyF3I CTNAG 6 cut(s) 255, 321, 420, 470, 489, 750
Hsp92II CATG 4 cut(s) 211, 353, 410, 602
HspAI GCGC 3 cut(s) 69, 71, 467
Kzo9I GATC 2 cut(s) 94, 818
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 9 cut(s) 33, 128, 228, 242, 283, 287, 314, 714, 750
Lsp1109I GCAGC 1 cut(s) 743
LweI GCATC 1 cut(s) 718
MalI GATC 2 cut(s) 96, 820
MboI GATC 2 cut(s) 94, 818
MboII GAAGA 5 cut(s) 175, 523, 532, 535, 802
MflI RGATCY 2 cut(s) 94, 818
MhlI GDGCHC 2 cut(s) 189, 290
MlyI GAGTC 3 cut(s) 42, 260, 494
MroXI GAANNNNTTC 2 cut(s) 125, 179
MseI TTAA 6 cut(s) 192, 200, 234, 309, 717, 798
MslI CAYNNNNRTG 1 cut(s) 608
MspI CCGG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 556
MvnI CGCG 2 cut(s) 71, 464
MwoI GCNNNNNNNGC 2 cut(s) 728, 737
NdeII GATC 2 cut(s) 94, 818
NlaIII CATG 4 cut(s) 211, 353, 410, 602
NlaIV GGNNCC 4 cut(s) 96, 138, 171, 213
NmeAIII GCCGAG 2 cut(s) 34, 368
NspI RCATGY 1 cut(s) 353
PauI GCGCGC 1 cut(s) 69
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 36
PctI GAATGC 1 cut(s) 556
PdmI GAANNNNTTC 2 cut(s) 125, 179
PfeI GAWTC 1 cut(s) 245
Pfl23II CGTACG 1 cut(s) 36
PkrI GCNGC 2 cut(s) 466, 733
PleI GAGTC 3 cut(s) 42, 259, 493
PpsI GAGTC 3 cut(s) 42, 259, 493
PspFI CCCAGC 1 cut(s) 736
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 4 cut(s) 96, 138, 171, 213
PspPI GGNCC 2 cut(s) 272, 698
PsuI RGATCY 2 cut(s) 94, 818
PteI GCGCGC 1 cut(s) 69
RsaI GTAC 3 cut(s) 5, 38, 592
RsaNI GTAC 3 cut(s) 4, 37, 591
RseI CAYNNNNRTG 1 cut(s) 608
Rsr2I CGGWCCG 1 cut(s) 272
RsrII CGGWCCG 1 cut(s) 272
SalI GTCGAC 1 cut(s) 29
SaqAI TTAA 6 cut(s) 192, 200, 234, 309, 717, 798
SatI GCNGC 2 cut(s) 465, 732
Sau3AI GATC 2 cut(s) 94, 818
Sau96I GGNCC 2 cut(s) 272, 698
SchI GAGTC 3 cut(s) 42, 260, 494
SduI GDGCHC 2 cut(s) 189, 290
SfaNI GCATC 1 cut(s) 718
SinI GGWCC 2 cut(s) 272, 698
SmiMI CAYNNNNRTG 1 cut(s) 608
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 5 cut(s) 345, 464, 554, 704, 722
StuI AGGCCT 1 cut(s) 152
TaqI TCGA 6 cut(s) 30, 230, 503, 578, 767, 782
TaqII GACCGA 1 cut(s) 289
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 6 cut(s) 192, 200, 234, 309, 717, 798
Tru9I TTAA 6 cut(s) 192, 200, 234, 309, 717, 798
TscAI CASTG 1 cut(s) 290
TseI GCWGC 1 cut(s) 731
TspRI CASTG 1 cut(s) 290
VpaK11BI GGWCC 2 cut(s) 272, 698
XceI RCATGY 1 cut(s) 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 30
XmnI GAANNNNTTC 2 cut(s) 125, 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.