AT5G58050

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013180
Physical Location & Seq
Reverse (-)
23494249 .. 23497601
3353 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58050.1

Sequence Viewer

Length: 2262 bp
ATGCTGAGGTTTTTCATTCTTTTCTCCCTTTTCTTACACTCTTCTGTTGCGGCACCCAAGACTCCTGCTGCTGCCGCAGCTGTTCCTGCCAAGAAATGGCTTACCCTTAACGGACAAGAGCCTGCCGTTGTGGCCAGAGGGGGCTTCTCGGGTCTTTTCCCTGAGTCAAGCATCTCTGCAAACGACTTGGCTATTGGCACCAGCTCGCCTGGCTTCACAATGTTGTGCAACCTTCAGATGACTAAGGACGGTGTGGGCCTTTGCTTGTCAGATATCAGACTTGACAATGCAACCACCATCTCCTCGGTCTTCCCCAAAGCCCAGAAGACCTACAAGGTCAACGGTCAGGACCTCAAGGGCTGGTTTGTCATTGATTACGATGCCGACACCATATTCAACAAAGTCACTCTGGTCCAGAACATCTTCTCTCGGCCTAGCATCTTCGACGGCCAAATGTCGGTTTCTGCAGTGGAGGATGTCCTTGGAACCAAGCCTCCCAAGTTCTGGTTGAGCGTTCAGTACGATGCCTTCTACATGGAACACAAGCTGAGCCCAGCCGAGTACCTGAGAAGCTTGCGATTCCGCGGCATTAATGTCATCTCATCTCCAGAGATTGGTTTCTTGAAGAGCATCGGGATGGACGCGGGCAGGGCCAAGACAAAGCTCATATTTGAGTTCAAAGACCCCGAGGCAGTTGAGCCCACCACCAACAAGAAGTACAGCGAGATTCAGCAGAACCTCGCAGCCATCAAGGCCTTTGCTTCAGGCGTTCTTGTGCCCAAAGACTACATTTGGCCCATTGACTCAGCTAAGTACCTTAAGCCCGCCACCACTTTTGTGGCTGATGCTCACAAAGCCGGTCTAGAGGTCTATGCTTCAGGGTTTGCCAACGACTTGCGCACCAGCTTTAACTACAGCTATGATCCCTCTGCTGAGTATCTCCAGTTTGTGGACAATGGCCAGTTCTCTGTTGATGGTGTCATCACCGATTTCCCGCCAACAGCATCACAATCAATCACTTGCTTTTCTCACCAAAACGGCAATCTCCCCAAAGCAGGGCATGCATTGGTTATAACTCACAATGGAGCAAGCGGAGACTATCCAGGCTGCACTGATCTGGCTTACCAGAAAGCAATCGACGATGGAGCAGACATAATTGACTGCTCTGTTCAGATGTCCAAAGACGGAATCGCTTTCTGCCATGATGCTGCAGACCTCTCAGCAAGTACCACCGCCAGGACTACTTTCATGTCCCGAGCCACCAGCGTTCCTGAGATCCAGCCTACCAACGGAATCTTCTCTTTTGATCTTACATGGGCCGAGATCCAGTCTGTGAAGCCACAAATAGAAAACCCCTTCACTGCCACGGGCTTCCAGAGAAACCCCGCAAACAAGAACGCCGGGAAGTTCACGACTCTTGCGGACTTCCTTGAGCTCGGCAAAGCAAAGGCAGTCACCGGAGTTTTAATCAACATTCAGAATGCTGCTTATTTAGCCTCGAAGAAAGGCTTAGGAGTCGTAGACGTAGTCAAGTCTGCTCTTACAAATTCCACACTCGACAAGCAATCAACCCAGAAGGTTTTGATTCAGTCGGATGACAGTTCAGTTCTATCTAGTTTCGAGGCTGTCCCTCCTTACACAAGAGTCTTGAGTATCGACAAGGAGATCGGAGATGCTCCTAAGACATCCATCGAAGAAATCAAGAAGCATGCAGATGCTGTCAATCTCTTGAGAACTTCCCTCATCACCGTCTCCCAAAGCTTTGCCACGGGGAAAACTAATGTGGTAGAGGAAATGCACAAAGCGAATATCTCTGTATACGTCTCGGTGCTAAGAAATGAGTACATCGCCATAGCGTTCGACTACTTCTCTGATCCTACGATAGAGCTTGCAACATTCATTGCAGGGAGAGGCGTTGATGGAGTCATCACGGAGTTCCCTGCCACCGCTACCAGATACCTAAGGAGTCCATGTTCAGATTTGAACAAAGACCAGCCCTATGCCATCTTACCTGCAGATGCTGGAGCTCTACTCACCGTGGCAGACAAGGAAGCACAACTACCAGCTATTCCCCCAAACCCACCTCTTGATGCCAAAGACGTGATTGATCCGCCTCTGCCTCCAGTTGCAAAGTTGGCCTCCAATGGAACTGAAGGTGGTCCGCCACAAACTCCTCCTCGTTCAGGCACCGTTGCCATTGCTGCTAATCTCAGCCTTTCCTTGCTGGCAATGATGGCTTTGGGACTCCTTTATACAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0016020 GO:0031224 GO:0031225 GO:0044425 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GDPD

Protein Analysis

753

Amino Acids

80.9

Weight (kDa)

5.47

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 360 - 648 2e-26 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58050 AT5G58170
fragaria_vesca FvH4_1g01240
malus_domestica MD02G1010300.v1.1 MD15G1155300.v1.1
prunus_persica Prupe.7G259800_v2.0.a1 Prupe.7G259800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086011
rosa_laevigata RLG00000015734
rosa_multiflora Rmu_ssc0000355.1_g000023
rosa_roxburghii Rroxscaffold_2G00154850
rosa_rugosa Rorug01G0465000 Rorug01G0465100
rosa_samantha Rh2AG014300 Rh2BG014800 Rh2CG015400 Rh2DG016700
rosa_wichuraiana Rw2G001270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1073
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 2020
AccB1I GGYRCC 3 cut(s) 52, 197, 2186
AccB7I CCANNNNNTGG 4 cut(s) 96, 504, 614, 949
AccI GTMKAC 2 cut(s) 1521, 1818
AccII CGCG 2 cut(s) 585, 644
AclWI GGATC 5 cut(s) 917, 1270, 1318, 1868, 2102
AcoI YGGCCR 3 cut(s) 132, 448, 958
AcsI RAATTY 1 cut(s) 1546
AcuI CTGAAG 4 cut(s) 218, 747, 861, 2172
AfaI GTAC 6 cut(s) 521, 563, 719, 815, 1228, 1844
AflII CTTAAG 1 cut(s) 818
AgsI TTSAA 4 cut(s) 397, 625, 679, 1984
AjiI CACGTC 1 cut(s) 2101
AjnI CCWGG 3 cut(s) 208, 1102, 1235
AjuI GAANNNNNNNTTGG 2 cut(s) 1280, 1312
AleI CACNNNNGTG 1 cut(s) 836
AloI GAACNNNNNNTCC 4 cut(s) 478, 510, 1957, 1989
Alw21I GWGCWC 2 cut(s) 1437, 2029
Alw26I GTCTC 3 cut(s) 1089, 1756, 1828
AlwI GGATC 5 cut(s) 917, 1270, 1318, 1868, 2102
AlwNI CAGNNNCTG 2 cut(s) 1718, 2021
Ama87I CYCGRG 3 cut(s) 148, 686, 1254
ApeKI GCWGC 8 cut(s) 68, 71, 77, 743, 1107, 1208, 1484, 2201
ApoI RAATTY 1 cut(s) 1546
AseI ATTAAT 1 cut(s) 591
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 900
AspS9I GGNCC 7 cut(s) 256, 349, 412, 651, 795, 1317, 2159
AsuC2I CCSGG 1 cut(s) 1402
AsuHPI GGTGA 5 cut(s) 976, 1022, 1447, 1738, 2026
AvaI CYCGRG 3 cut(s) 148, 686, 1254
AvaII GGWCC 3 cut(s) 349, 412, 2159
AxyI CCTNAGG 1 cut(s) 1961
BaeGI GKGCMC 1 cut(s) 780
BalI TGGCCA 2 cut(s) 134, 960
BanI GGYRCC 3 cut(s) 52, 197, 2186
BanII GRGCYC 4 cut(s) 554, 702, 1437, 2029
BarI GAAGNNNNNNTAC 4 cut(s) 512, 544, 2043, 2075
BbsI GAAGAC 2 cut(s) 301, 332
Bbv12I GWGCWC 2 cut(s) 1437, 2029
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 8 cut(s) 55, 58, 89, 755, 1094, 1195, 1471, 2188
BccI CCATC 9 cut(s) 305, 631, 755, 968, 1136, 1697, 1913, 2012, 2227
BceAI ACGGC 3 cut(s) 110, 463, 1054
BciT130I CCWGG 3 cut(s) 210, 1104, 1237
BcnI CCSGG 1 cut(s) 1402
BcoDI GTCTC 3 cut(s) 1089, 1756, 1828
BfaI CTAG 3 cut(s) 435, 863, 1614
BfmI CTRYAG 4 cut(s) 465, 913, 1209, 2013
BfrI CTTAAG 1 cut(s) 818
BfuAI ACCTGC 1 cut(s) 2020
BglI GCCNNNNNGGC 2 cut(s) 131, 752
BlpI GCTNAGC 1 cut(s) 548
Bme1390I CCNGG 4 cut(s) 210, 1104, 1237, 1402
Bme18I GGWCC 3 cut(s) 349, 412, 2159
BmeT110I CYCGRG 3 cut(s) 148, 686, 1254
BmgBI CACGTC 1 cut(s) 2101
BmgT120I GGNCC 7 cut(s) 256, 349, 412, 651, 795, 1317, 2159
BmiI GGNNCC 4 cut(s) 54, 199, 487, 2188
BmrFI CCNGG 4 cut(s) 210, 1104, 1237, 1402
BpiI GAAGAC 2 cut(s) 301, 332
BplI GAGNNNNNCTC 2 cut(s) 2016, 2048
BpmI CTGGAG 4 cut(s) 591, 926, 2043, 2106
Bpu10I CCTNAGC 2 cut(s) 5, 1510
Bpu1102I GCTNAGC 1 cut(s) 548
BpuEI CTTGAG 4 cut(s) 338, 1451, 1669, 1750
BpuMI CCSGG 1 cut(s) 1402
BsaJI CCNNGG 7 cut(s) 303, 481, 583, 687, 1365, 1767, 2037
BsaWI WCCGGW 1 cut(s) 1457
BsaXI ACNNNNNCTCC 4 cut(s) 478, 508, 1957, 1987
Bse118I RCCGGY 1 cut(s) 857
Bse1I ACTGG 4 cut(s) 943, 961, 1327, 2123
Bse21I CCTNAGG 1 cut(s) 1961
Bse3DI GCAATG 3 cut(s) 1899, 2196, 2235
BseBI CCWGG 3 cut(s) 210, 1104, 1237
BseDI CCNNGG 7 cut(s) 303, 481, 583, 687, 1365, 1767, 2037
BseGI GGATG 4 cut(s) 481, 642, 1600, 1685
BseMI GCAATG 3 cut(s) 1899, 2196, 2235
BseMII CTCAG 8 cut(s) 153, 539, 557, 819, 924, 1233, 1263, 2224
BseNI ACTGG 4 cut(s) 943, 961, 1327, 2123
BseRI GAGGAG 3 cut(s) 292, 2163, 2166
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 8 cut(s) 55, 58, 89, 755, 1094, 1195, 1471, 2188
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 1 cut(s) 1093
Bsh1236I CGCG 2 cut(s) 585, 644
BshNI GGYRCC 3 cut(s) 52, 197, 2186
BsiHKAI GWGCWC 2 cut(s) 1437, 2029
BsiHKCI CYCGRG 3 cut(s) 148, 686, 1254
BsiSI CCGG 3 cut(s) 858, 1401, 1458
BslFI GGGAC 3 cut(s) 1237, 1613, 2256
BsmAI GTCTC 3 cut(s) 1089, 1756, 1828
BsmBI CGTCTC 2 cut(s) 1756, 1828
BsmFI GGGAC 3 cut(s) 1237, 1613, 2256
BsmI GAATGC 1 cut(s) 1486
BsoBI CYCGRG 3 cut(s) 148, 686, 1254
Bsp1286I GDGCHC 5 cut(s) 554, 702, 780, 1437, 2029
Bsp143I GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
Bsp1720I GCTNAGC 1 cut(s) 548
BspCNI CTCAG 8 cut(s) 154, 540, 558, 818, 925, 1232, 1264, 2223
BspFNI CGCG 2 cut(s) 585, 644
BspLI GGNNCC 4 cut(s) 54, 199, 487, 2188
BspMAI CTGCAG 3 cut(s) 469, 1213, 2017
BspMI ACCTGC 1 cut(s) 2020
BspPI GGATC 5 cut(s) 917, 1270, 1318, 1868, 2102
BspQI GCTCTTC 1 cut(s) 620
BspT107I GGYRCC 3 cut(s) 52, 197, 2186
BspTI CTTAAG 1 cut(s) 818
BsrDI GCAATG 3 cut(s) 1899, 2196, 2235
BsrFI RCCGGY 1 cut(s) 857
BsrI ACTGG 4 cut(s) 943, 961, 1327, 2123
BssAI RCCGGY 1 cut(s) 857
BssECI CCNNGG 7 cut(s) 303, 481, 583, 687, 1365, 1767, 2037
BssMI GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
BssNAI GTATAC 1 cut(s) 1819
BssT1I CCWWGG 1 cut(s) 481
Bst1107I GTATAC 1 cut(s) 1819
Bst2UI CCWGG 3 cut(s) 210, 1104, 1237
Bst4CI ACNGT 6 cut(s) 251, 344, 1601, 1750, 2038, 2191
Bst6I CTCTTC 2 cut(s) 46, 620
BstAFI CTTAAG 1 cut(s) 818
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstDSI CCRYGG 4 cut(s) 583, 1365, 1767, 2037
BstENI CCTNNNNNAGG 1 cut(s) 2181
BstF5I GGATG 4 cut(s) 481, 642, 1600, 1685
BstFNI CGCG 2 cut(s) 585, 644
BstHHI GCGC 1 cut(s) 900
BstKTI GATC 8 cut(s) 925, 1117, 1278, 1309, 1326, 1668, 1876, 2110
BstMAI GTCTC 3 cut(s) 1089, 1756, 1828
BstMBI GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
BstNI CCWGG 3 cut(s) 210, 1104, 1237
BstNSI RCATGY 2 cut(s) 1064, 1712
BstSCI CCNGG 4 cut(s) 208, 1102, 1235, 1400
BstSFI CTRYAG 4 cut(s) 465, 913, 1209, 2013
BstSLI GKGCMC 1 cut(s) 780
BstUI CGCG 2 cut(s) 585, 644
BstV1I GCAGC 8 cut(s) 55, 58, 89, 755, 1094, 1195, 1471, 2188
BstV2I GAAGAC 2 cut(s) 301, 332
BstX2I RGATCY 2 cut(s) 1275, 1323
BstXI CCANNNNNNTGG 1 cut(s) 838
BstYI RGATCY 2 cut(s) 1275, 1323
BstZ17I GTATAC 1 cut(s) 1819
Bsu36I CCTNAGG 1 cut(s) 1961
BtgI CCRYGG 4 cut(s) 583, 1365, 1767, 2037
BtgZI GCGATG 1 cut(s) 1831
BtrI CACGTC 1 cut(s) 2101
BtsCI GGATG 4 cut(s) 481, 642, 1600, 1685
BtsI GCAGTG 2 cut(s) 474, 1359
BtsIMutI CAGTG 3 cut(s) 474, 1110, 1359
BveI ACCTGC 1 cut(s) 2020
CaiI CAGNNNCTG 2 cut(s) 1718, 2021
CfoI GCGC 1 cut(s) 900
Cfr10I RCCGGY 1 cut(s) 857
Cfr13I GGNCC 7 cut(s) 256, 349, 412, 651, 795, 1317, 2159
Cfr42I CCGCGG 1 cut(s) 586
CseI GACGC 1 cut(s) 650
Csp6I GTAC 6 cut(s) 520, 562, 718, 814, 1227, 1843
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 7 cut(s) 535, 1061, 1202, 1249, 1314, 1709, 1971
CviQI GTAC 6 cut(s) 520, 562, 718, 814, 1227, 1843
DpnI GATC 8 cut(s) 924, 1116, 1277, 1308, 1325, 1667, 1875, 2109
DpnII GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
EaeI YGGCCR 3 cut(s) 132, 448, 958
Eam1104I CTCTTC 2 cut(s) 46, 620
EarI CTCTTC 2 cut(s) 46, 620
EciI GGCGGA 2 cut(s) 2100, 2151
Ecl136II GAGCTC 2 cut(s) 1435, 2027
Eco130I CCWWGG 1 cut(s) 481
Eco147I AGGCCT 1 cut(s) 755
Eco24I GRGCYC 4 cut(s) 554, 702, 1437, 2029
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 3 cut(s) 349, 412, 2159
Eco53kI GAGCTC 2 cut(s) 1435, 2027
Eco57I CTGAAG 4 cut(s) 218, 747, 861, 2172
Eco81I CCTNAGG 1 cut(s) 1961
Eco88I CYCGRG 3 cut(s) 148, 686, 1254
EcoICRI GAGCTC 2 cut(s) 1435, 2027
EcoNI CCTNNNNNAGG 1 cut(s) 2181
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 208, 1102, 1235
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 4 cut(s) 554, 702, 1437, 2029
ErhI CCWWGG 1 cut(s) 481
Esp3I CGTCTC 2 cut(s) 1756, 1828
FaeI CATG 7 cut(s) 538, 1064, 1205, 1252, 1317, 1712, 1974
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 3 cut(s) 1237, 1613, 2256
FatI CATG 7 cut(s) 534, 1060, 1201, 1248, 1313, 1708, 1970
FauI CCCGC 4 cut(s) 637, 832, 1002, 1393
FblI GTMKAC 2 cut(s) 1521, 1818
FokI GGATG 4 cut(s) 488, 649, 1607, 1672
FriOI GRGCYC 4 cut(s) 554, 702, 1437, 2029
FspBI CTAG 3 cut(s) 435, 863, 1614
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 1 cut(s) 899
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 4 cut(s) 591, 926, 2043, 2106
HapII CCGG 3 cut(s) 858, 1401, 1458
HgaI GACGC 1 cut(s) 650
HhaI GCGC 1 cut(s) 900
Hin1II CATG 7 cut(s) 538, 1064, 1205, 1252, 1317, 1712, 1974
Hin6I GCGC 1 cut(s) 898
HinP1I GCGC 1 cut(s) 898
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 2 cut(s) 571, 1759
HpaII CCGG 3 cut(s) 858, 1401, 1458
HphI GGTGA 5 cut(s) 976, 1022, 1447, 1738, 2026
Hpy166II GTNNAC 5 cut(s) 340, 952, 1410, 1522, 1819
Hpy188I TCNGA 9 cut(s) 237, 271, 278, 1173, 1479, 1594, 1670, 1873, 1978
Hpy8I GTNNAC 5 cut(s) 340, 952, 1410, 1522, 1819
Hpy99I CGWCG 2 cut(s) 449, 1142
HpyAV CCTTC 5 cut(s) 242, 538, 1366, 1570, 2147
HpyCH4III ACNGT 6 cut(s) 251, 344, 1601, 1750, 2038, 2191
HpyCH4IV ACGT 3 cut(s) 1524, 1821, 2100
HpySE526I ACGT 3 cut(s) 1524, 1821, 2100
Hsp92II CATG 7 cut(s) 538, 1064, 1205, 1252, 1317, 1712, 1974
HspAI GCGC 1 cut(s) 898
KspI CCGCGG 1 cut(s) 586
Kzo9I GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 4 cut(s) 1085, 1145, 1681, 2024
Lsp1109I GCAGC 8 cut(s) 55, 58, 89, 755, 1094, 1195, 1471, 2188
MaeI CTAG 3 cut(s) 435, 863, 1614
MaeII ACGT 3 cut(s) 1524, 1821, 2100
MaeIII GTNAC 2 cut(s) 403, 1453
MalI GATC 8 cut(s) 924, 1116, 1277, 1308, 1325, 1667, 1875, 2109
MboI GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
MboII GAAGA 9 cut(s) 33, 301, 337, 415, 433, 637, 1288, 1513, 1706
MflI RGATCY 2 cut(s) 1275, 1323
MhlI GDGCHC 5 cut(s) 554, 702, 780, 1437, 2029
MlsI TGGCCA 2 cut(s) 134, 960
MluCI AATT 2 cut(s) 1155, 1546
MluNI TGGCCA 2 cut(s) 134, 960
MlyI GAGTC 9 cut(s) 55, 173, 797, 1408, 1524, 1653, 1932, 1975, 2238
MmeI TCCRAC 1 cut(s) 1572
Mox20I TGGCCA 2 cut(s) 134, 960
Mph1103I ATGCAT 1 cut(s) 1066
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 2 cut(s) 134, 960
MseI TTAA 5 cut(s) 108, 591, 819, 909, 1466
MslI CAYNNNNRTG 3 cut(s) 635, 836, 1713
Msp20I TGGCCA 2 cut(s) 134, 960
MspA1I CMGCKG 2 cut(s) 80, 585
MspCI CTTAAG 1 cut(s) 818
MspI CCGG 3 cut(s) 858, 1401, 1458
MspR9I CCNGG 4 cut(s) 210, 1104, 1237, 1402
Mva1269I GAATGC 1 cut(s) 1486
MvaI CCWGG 3 cut(s) 210, 1104, 1237
MvnI CGCG 2 cut(s) 585, 644
NciI CCSGG 1 cut(s) 1402
NdeII GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
NlaIII CATG 7 cut(s) 538, 1064, 1205, 1252, 1317, 1712, 1974
NlaIV GGNNCC 4 cut(s) 54, 199, 487, 2188
NmeAIII GCCGAG 4 cut(s) 409, 583, 1345, 1416
NmuCI GTSAC 2 cut(s) 403, 1453
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 2 cut(s) 1064, 1712
OliI CACNNNNGTG 1 cut(s) 836
PaeI GCATGC 2 cut(s) 1064, 1712
PceI AGGCCT 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 620
PctI GAATGC 1 cut(s) 1486
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 5 cut(s) 579, 727, 1188, 1293, 1585
PflFI GACNNNGTC 1 cut(s) 1526
PflMI CCANNNNNTGG 4 cut(s) 96, 504, 614, 949
PleI GAGTC 9 cut(s) 55, 172, 797, 1408, 1523, 1652, 1931, 1974, 2238
PpsI GAGTC 9 cut(s) 55, 172, 797, 1408, 1523, 1652, 1931, 1974, 2238
PpuMI RGGWCCY 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 591
PsiI TTATAA 1 cut(s) 1073
Psp124BI GAGCTC 2 cut(s) 1437, 2029
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 3 cut(s) 208, 1102, 1235
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 208, 1102, 1235
PspN4I GGNNCC 4 cut(s) 54, 199, 487, 2188
PspPI GGNCC 7 cut(s) 256, 349, 412, 651, 795, 1317, 2159
PspPPI RGGWCCY 1 cut(s) 349
PstI CTGCAG 3 cut(s) 469, 1213, 2017
PstNI CAGNNNCTG 2 cut(s) 1718, 2021
PsuI RGATCY 2 cut(s) 1275, 1323
PsyI GACNNNGTC 1 cut(s) 1526
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 6 cut(s) 521, 563, 719, 815, 1228, 1844
RsaNI GTAC 6 cut(s) 520, 562, 718, 814, 1227, 1843
RseI CAYNNNNRTG 3 cut(s) 635, 836, 1713
SacI GAGCTC 2 cut(s) 1437, 2029
SacII CCGCGG 1 cut(s) 586
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 5 cut(s) 108, 591, 819, 909, 1466
Sau3AI GATC 8 cut(s) 922, 1114, 1275, 1306, 1323, 1665, 1873, 2107
Sau96I GGNCC 7 cut(s) 256, 349, 412, 651, 795, 1317, 2159
SchI GAGTC 9 cut(s) 55, 173, 797, 1408, 1524, 1653, 1932, 1975, 2238
ScrFI CCNGG 4 cut(s) 210, 1104, 1237, 1402
SduI GDGCHC 5 cut(s) 554, 702, 780, 1437, 2029
SfcI CTRYAG 4 cut(s) 465, 913, 1209, 2013
Sfr303I CCGCGG 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 586
SinI GGWCC 3 cut(s) 349, 412, 2159
SmiMI CAYNNNNRTG 3 cut(s) 635, 836, 1713
SmlI CTYRAG 5 cut(s) 353, 818, 1430, 1648, 1729
SmoI CTYRAG 5 cut(s) 353, 818, 1430, 1648, 1729
SphI GCATGC 2 cut(s) 1064, 1712
Sse9I AATT 2 cut(s) 1155, 1546
SseBI AGGCCT 1 cut(s) 755
SspMI CTAG 3 cut(s) 435, 863, 1614
SstI GAGCTC 2 cut(s) 1437, 2029
StuI AGGCCT 1 cut(s) 755
StyD4I CCNGG 4 cut(s) 208, 1102, 1235, 1400
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 6 cut(s) 251, 344, 1601, 1750, 2038, 2191
TaiI ACGT 3 cut(s) 1527, 1824, 2103
TaqI TCGA 8 cut(s) 444, 1137, 1499, 1557, 1620, 1656, 1692, 1860
TaqII GACCGA 1 cut(s) 295
TasI AATT 2 cut(s) 1155, 1546
TatI WGTACW 2 cut(s) 717, 1842
TauI GCSGC 3 cut(s) 53, 77, 588
TfiI GAWTC 5 cut(s) 579, 727, 1188, 1293, 1585
Tru1I TTAA 5 cut(s) 108, 591, 819, 909, 1466
Tru9I TTAA 5 cut(s) 108, 591, 819, 909, 1466
TscAI CASTG 3 cut(s) 474, 1117, 1366
TseFI GTSAC 2 cut(s) 403, 1453
TseI GCWGC 8 cut(s) 68, 71, 77, 743, 1107, 1208, 1484, 2201
Tsp45I GTSAC 2 cut(s) 403, 1453
TspDTI ATGAA 3 cut(s) 4, 1237, 1888
TspGWI ACGGA 4 cut(s) 126, 1200, 1305, 1946
TspRI CASTG 3 cut(s) 474, 1117, 1366
Tth111I GACNNNGTC 1 cut(s) 1526
Van91I CCANNNNNTGG 4 cut(s) 96, 504, 614, 949
Vha464I CTTAAG 1 cut(s) 818
VpaK11BI GGWCC 3 cut(s) 349, 412, 2159
VspI ATTAAT 1 cut(s) 591
XagI CCTNNNNNAGG 1 cut(s) 2181
XapI RAATTY 1 cut(s) 1546
XbaI TCTAGA 1 cut(s) 862
XceI RCATGY 2 cut(s) 1064, 1712
XcmI CCANNNNNNNNNTGG 1 cut(s) 835
XmiI GTMKAC 2 cut(s) 1521, 1818
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 3 cut(s) 435, 863, 1614
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.