MD02G1010300.v1.1

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013180
Physical Location & Seq
Reverse (-)
675664 .. 679615
3952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1010300.v1.1.491

Sequence Viewer

Length: 2340 bp
ATGATCAGGTGTTTGCTTTTCGTTTCCCTGTTGATCCACTCGTCTTTGGCAGCAAAACCACCAAGTGAAGGACAGAAAGCAGCACCTCAGGCTCAGGGGCCTCCCTCGAAAAAATGGCAGACTTTAACCGGTCAACAGCCTCTTGTTATAGCGCGCGGCGGGCTTTCAGGGCTGTTCCCGGAAGCCAGCACCTACGCGAATAATATGGCAAAGACCCTGAGTTTGCAGGGCTCTGCCTTCTTCTGCAATCTGCAGTTAACGAAGGATAGCGTCGGAATATGCCTGTCGAACATTCTGCTTGATAATACAACAACTATATCTATGTTCTACCCTAAGGGCCAAAAAACTTACAATGTGAACGGGAAGGATATTCGCGGCTGGTTTGCGTTGGATTTCACAGCTGATCAGGTGTTAAACAATGTGTCACTGACTCAAAACATTTATTCGCGACCAAGTCAGTTCGATGGTATTTTGCCAATCGCTACTGTCGAAGATGCTTTGGGAGCTAAGCCAGATAAATTTTGGTTGAATGTGCCGTATGATGCATTTTACAACGAACACAAGATCAGCATGGCATCATTTGTCGAAAAGGCAGCCAAATTTGCACCTATACCTTACATCTCATCTCCTGAGATCGGATTCTTGAAAGCCATCGGTGGAAAAGTGAACAAAATGAGGACAAAGCTCATCTTCCAGTTCCTTGACCCCGAAGAAACAGAACCGACAACCAAGCAAAAGTACAGTGCGCTGGTGAAGCAGCTCGGAATGATCAAGTCAGTTGCAGCCGGAATCCTTGTACCAAAAGAATACATATGGCCTGTAAACAAAAACAATTACGTGGAGAAAGCCACGACACTCGTGAATGATGCTCACAAAGAAGGCCTAGAAGTGCATGCTTCCGGTTTTGCCAATGATGTTATTCTCCCTTATAACTACAGTTTTGATCCCACAGCTGAGTACCTGCAGTTTGTCGATAACTCTCAGTTTTCTGTTGATGGTGTGCTGACCGATTTCCCTCCTACAGCATCAGAAGCAATCGCGTGCTTTACGCAGAACAAGAACGAAACCAAGCCTAAGACAGGATTTCCTTTGATCATAACTAGAAATGGAGCTAGTGGAATCTATCCCGGCTGCACAGACCTTGCTTACAAGCAGGCAGTAGAGGATGGGGCTGACATAATCGATTGCTCGGTTCAAATGTCAAGTGATGGTGTTGCCTTCTGCTTAGACACTCCAGACCTCAACTCGGGCACTAACGCATTGAGCCAATTCATGACCAAGAGTGAGACCATCCCCGAACTTCAAGCAGAAGCCGGAGTCTTCTCCTTCCAACTCTCGTGGAGCGATATTAAGAGCTTGAAACCACAAATGCAGAGCCCCTACGGAACCAAGGAAAAAATAGTCAGAAATCCGGCTAACAAGGACGCGGGCAAACTGGTGACCCTCAATGAATTCCTGGAGTTTGCAAAGAAAAATGCAGTTACTGGAATTTTGATCAACATTGAGTATGCTGCATACCTAGCATCAAAGGCAGGACTAGACGTTGTGGGAACTGTATCCTCTGCCTTGAGCAATGCCACATTCGACAAGCAATCCACTCAGCAAGTGTTGATCGAGTCGGATGACACTTCCGTGCTGGCCAAGTTTAAAGACGCGAAAAACTACAAGCGAGTGCTCAACATCAAGGAGAAGATAAGCGAAGCAACCAAGGAATCTGTGGCGGAGATCAAGAAGTACGCAGATGCAGTAACCATCACCAAGGATTCGATCGTCAAGATCACCGATTACTTCATTTCTGGCACGACCAACGTCGTCAAGGAAATGAAGGCCGCGAACATCACTGTGTACGCCCACTGGCTGAGGAACGAGTTCGTCAGTCTCATGTTCGATTATTTCTCAGACCCCATCACTGAGATTGCTTCATACGCTCAAGGGTTCAAGGTTGATGGCATTGTCACCGATTTCCCCGGAACTGCCAGCAAATATATGAGATCCCCATGCAGTGATACGGACCCCAAGGCCAACGTCCCGTATCCAATCTTGCCAGTTGAACCAGGTGCACTGTCGGGTCTGATCGGCCCCGAGGCATTGTCTCCGGCGGAAGCCCCGAGCCCGGCTCTTCAGGTCTCGAATGTGGTTGACCCACCGCTCCCCGGAGTGGCGAAGAAGAACGACTCCAGCTCCAGCCCCGGCTCCGGCTCCAACTCGGGTTCATCTGATGCAGGCTCCAAATCTCCACCTGCTCCTGCACCTTCTGATGAAAAGTCAAGTGCTTTTGCGAATGGGGCCAGTTGCTGTGCTTCTCTAGTTGCTGTAACGGTGCTTTTGCTGGCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0016020 GO:0031224 GO:0031225 GO:0044425 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GDPD

Protein Analysis

780

Amino Acids

83.88

Weight (kDa)

5.51

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 84 - 339 3.8e-06 Glycerophosphoryl diester phosphodiesterase family
GDPD PF03009 370 - 658 8e-23 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58050 AT5G58170
fragaria_vesca FvH4_1g01240
malus_domestica MD02G1010300.v1.1 MD15G1155300.v1.1
prunus_persica Prupe.7G259800_v2.0.a1 Prupe.7G259800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086011
rosa_laevigata RLG00000015734
rosa_multiflora Rmu_ssc0000355.1_g000023
rosa_roxburghii Rroxscaffold_2G00154850
rosa_rugosa Rorug01G0465000 Rorug01G0465100
rosa_samantha Rh2AG014300 Rh2BG014800 Rh2CG015400 Rh2DG016700
rosa_wichuraiana Rw2G001270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AarI CACCTGC 1 cut(s) 2248
Acc36I ACCTGC 2 cut(s) 969, 2248
AccBSI CCGCTC 1 cut(s) 2149
AccII CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
AciI CCGC 8 cut(s) 156, 159, 375, 1427, 1721, 1830, 2099, 2147
AclWI GGATC 3 cut(s) 28, 938, 1986
AcoI YGGCCR 1 cut(s) 1638
AcsI RAATTY 4 cut(s) 518, 599, 1451, 1488
AcuI CTGAAG 1 cut(s) 2105
AdeI CACNNNGTG 1 cut(s) 65
AfaI GTAC 5 cut(s) 740, 798, 959, 1736, 1847
AfiI CCNNNNNNNGG 8 cut(s) 68, 635, 1079, 1246, 2113, 2153, 2158, 2195
AgeI ACCGGT 1 cut(s) 128
AgsI TTSAA 7 cut(s) 529, 646, 1196, 1304, 1360, 1939, 2051
AjnI CCWGG 2 cut(s) 1455, 2053
AleI CACNNNNGTG 1 cut(s) 1631
AluBI AGCT 8 cut(s) 401, 506, 685, 760, 953, 1112, 1356, 2181
AluI AGCT 8 cut(s) 401, 506, 685, 760, 953, 1112, 1356, 2181
Alw21I GWGCWC 2 cut(s) 1677, 2062
Alw26I GTCTC 4 cut(s) 1280, 1883, 2097, 2131
Alw44I GTGCAC 1 cut(s) 2058
AlwI GGATC 3 cut(s) 28, 938, 1986
AlwNI CAGNNNCTG 2 cut(s) 1484, 2295
Ama87I CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
AoxI GGCC 9 cut(s) 98, 337, 815, 880, 1638, 1827, 2019, 2077, 2286
ApaLI GTGCAC 1 cut(s) 2058
ApeKI GCWGC 7 cut(s) 50, 80, 593, 757, 782, 1131, 1511
ApoI RAATTY 4 cut(s) 518, 599, 1451, 1488
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AsiGI ACCGGT 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 1868
AspLEI GCGC 3 cut(s) 154, 156, 748
AspS9I GGNCC 5 cut(s) 98, 337, 2011, 2078, 2286
AsuC2I CCSGG 6 cut(s) 179, 1128, 1968, 2114, 2154, 2190
AsuHPI GGTGA 5 cut(s) 763, 1450, 1747, 1771, 1948
AvaI CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
AvaII GGWCC 1 cut(s) 2011
AxyI CCTNAGG 2 cut(s) 87, 333
BaeGI GKGCMC 2 cut(s) 1253, 2062
BalI TGGCCA 1 cut(s) 1640
BanII GRGCYC 3 cut(s) 233, 1379, 2114
BauI CACGAG 2 cut(s) 857, 1336
BbsI GAAGAC 1 cut(s) 1312
Bbv12I GWGCWC 2 cut(s) 1677, 2062
BbvCI CCTCAGC 1 cut(s) 1859
BbvI GCAGC 7 cut(s) 62, 92, 605, 769, 794, 1118, 1498
BccI CCATC 9 cut(s) 458, 659, 989, 1160, 1202, 1298, 1760, 1913, 1940
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 2 cut(s) 1457, 2055
BciVI GTATCC 2 cut(s) 1567, 2043
BclI TGATCA 5 cut(s) 3, 403, 768, 1092, 1494
BcnI CCSGG 6 cut(s) 179, 1128, 1968, 2114, 2154, 2190
BcoDI GTCTC 4 cut(s) 1280, 1883, 2097, 2131
BfaI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2306
BfmI CTRYAG 4 cut(s) 251, 934, 962, 1020
BfuAI ACCTGC 2 cut(s) 969, 2248
BfuI GTATCC 2 cut(s) 1567, 2043
BlpI GCTNAGC 1 cut(s) 507
Bme1390I CCNGG 8 cut(s) 179, 1128, 1457, 1968, 2055, 2114, 2154, 2190
Bme18I GGWCC 1 cut(s) 2011
BmeT110I CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
BmgT120I GGNCC 5 cut(s) 98, 337, 2011, 2078, 2286
BmiI GGNNCC 8 cut(s) 99, 1387, 2013, 2080, 2194, 2200, 2227, 2287
BmrFI CCNGG 8 cut(s) 179, 1128, 1457, 1968, 2055, 2114, 2154, 2190
BmsI GCATC 8 cut(s) 484, 532, 584, 856, 1034, 1532, 1732, 2209
BoxI GACNNNNGTC 1 cut(s) 1808
BpiI GAAGAC 1 cut(s) 1312
BplI GAGNNNNNCTC 2 cut(s) 2101, 2133
BpmI CTGGAG 4 cut(s) 1218, 1478, 2161, 2167
Bpu10I CCTNAGC 2 cut(s) 93, 1859
Bpu1102I GCTNAGC 1 cut(s) 507
BpuEI CTTGAG 2 cut(s) 1588, 1914
BpuMI CCSGG 6 cut(s) 179, 1128, 1968, 2114, 2154, 2190
Bsa29I ATCGAT 1 cut(s) 1182
BsaAI YACGTR 1 cut(s) 838
BsaI GGTCTC 2 cut(s) 1280, 2131
BsaJI CCNNGG 8 cut(s) 1389, 1707, 1758, 1966, 2016, 2082, 2152, 2188
BsaWI WCCGGW 2 cut(s) 128, 899
BsaXI ACNNNNNCTCC 2 cut(s) 2165, 2195
Bsc4I CCNNNNNNNGG 8 cut(s) 68, 635, 1079, 1246, 2113, 2153, 2158, 2195
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 6 cut(s) 694, 1440, 1489, 1859, 2045, 2289
Bse21I CCTNAGG 2 cut(s) 87, 333
Bse3DI GCAATG 1 cut(s) 1579
BseBI CCWGG 2 cut(s) 1457, 2055
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 8 cut(s) 1389, 1707, 1758, 1966, 2016, 2082, 2152, 2188
BseGI GGATG 3 cut(s) 1171, 1290, 1627
BseLI CCNNNNNNNGG 8 cut(s) 68, 635, 1079, 1246, 2113, 2153, 2158, 2195
BseMI GCAATG 1 cut(s) 1579
BseNI ACTGG 6 cut(s) 694, 1440, 1489, 1859, 2045, 2289
BsePI GCGCGC 1 cut(s) 152
BseSI GKGCMC 2 cut(s) 1253, 2062
BseXI GCAGC 7 cut(s) 62, 92, 605, 769, 794, 1118, 1498
BsgI GTGCAG 2 cut(s) 1117, 2232
Bsh1236I CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
Bsh1285I CGRYCG 1 cut(s) 1770
BshFI GGCC 9 cut(s) 100, 339, 817, 882, 1640, 1829, 2021, 2079, 2288
BshTI ACCGGT 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 1182
BsiEI CGRYCG 1 cut(s) 1770
BsiHKAI GWGCWC 2 cut(s) 1677, 2062
BsiHKCI CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
BslFI GGGAC 1 cut(s) 2012
BslI CCNNNNNNNGG 8 cut(s) 68, 635, 1079, 1246, 2113, 2153, 2158, 2195
BsmAI GTCTC 4 cut(s) 1280, 1883, 2097, 2131
BsmFI GGGAC 1 cut(s) 2012
BsnI GGCC 9 cut(s) 100, 339, 817, 882, 1640, 1829, 2021, 2079, 2288
Bso31I GGTCTC 2 cut(s) 1280, 2131
BsoBI CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
Bsp1286I GDGCHC 6 cut(s) 233, 1253, 1379, 1677, 2062, 2114
Bsp1720I GCTNAGC 1 cut(s) 507
Bsp68I TCGCGA 1 cut(s) 448
BspACI CCGC 8 cut(s) 156, 159, 375, 1427, 1721, 1830, 2099, 2147
BspANI GGCC 9 cut(s) 100, 339, 817, 882, 1640, 1829, 2021, 2079, 2288
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
BspHI TCATGA 1 cut(s) 1272
BspLI GGNNCC 8 cut(s) 99, 1387, 2013, 2080, 2194, 2200, 2227, 2287
BspMAI CTGCAG 2 cut(s) 255, 966
BspMI ACCTGC 2 cut(s) 969, 2248
BspPI GGATC 3 cut(s) 28, 938, 1986
BspQI GCTCTTC 1 cut(s) 2124
BspTNI GGTCTC 2 cut(s) 1280, 2131
BsrBI CCGCTC 1 cut(s) 2149
BsrDI GCAATG 1 cut(s) 1579
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 6 cut(s) 694, 1440, 1489, 1859, 2045, 2289
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 8 cut(s) 1389, 1707, 1758, 1966, 2016, 2082, 2152, 2188
BssHII GCGCGC 1 cut(s) 152
BssSI CACGAG 2 cut(s) 857, 1336
BssT1I CCWWGG 4 cut(s) 1389, 1707, 1758, 2016
Bst2BI CACGAG 2 cut(s) 857, 1336
Bst2UI CCWGG 2 cut(s) 1457, 2055
Bst4CI ACNGT 7 cut(s) 487, 743, 938, 1555, 1843, 2064, 2320
Bst6I CTCTTC 1 cut(s) 2124
BstBAI YACGTR 1 cut(s) 838
BstEII GGTNACC 1 cut(s) 1438
BstENI CCTNNNNNAGG 1 cut(s) 1077
BstF5I GGATG 3 cut(s) 1171, 1290, 1627
BstFNI CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
BstHHI GCGC 3 cut(s) 154, 156, 748
BstMAI GTCTC 4 cut(s) 1280, 1883, 2097, 2131
BstMCI CGRYCG 1 cut(s) 1770
BstNI CCWGG 2 cut(s) 1457, 2055
BstNSI RCATGY 1 cut(s) 896
BstPAI GACNNNNGTC 1 cut(s) 1808
BstPI GGTNACC 1 cut(s) 1438
BstSCI CCNGG 8 cut(s) 177, 1126, 1455, 1966, 2053, 2112, 2152, 2188
BstSFI CTRYAG 4 cut(s) 251, 934, 962, 1020
BstSLI GKGCMC 2 cut(s) 1253, 2062
BstUI CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
BstV1I GCAGC 7 cut(s) 62, 92, 605, 769, 794, 1118, 1498
BstV2I GAAGAC 1 cut(s) 1312
BstX2I RGATCY 1 cut(s) 1991
BstYI RGATCY 1 cut(s) 1991
Bsu15I ATCGAT 1 cut(s) 1182
Bsu36I CCTNAGG 2 cut(s) 87, 333
BsuI GTATCC 2 cut(s) 1567, 2043
BsuRI GGCC 9 cut(s) 100, 339, 817, 882, 1640, 1829, 2021, 2079, 2288
BsuTUI ATCGAT 1 cut(s) 1182
BtsCI GGATG 3 cut(s) 1171, 1290, 1627
BtsI GCAGTG 1 cut(s) 2008
BtsIMutI CAGTG 7 cut(s) 425, 748, 1839, 1852, 1908, 2008, 2060
BtuMI TCGCGA 1 cut(s) 448
BveI ACCTGC 2 cut(s) 969, 2248
CaiI CAGNNNCTG 2 cut(s) 1484, 2295
CciI TCATGA 1 cut(s) 1272
CfoI GCGC 3 cut(s) 154, 156, 748
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 5 cut(s) 98, 337, 2011, 2078, 2286
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 3 cut(s) 259, 1433, 1661
CsiI ACCWGGT 1 cut(s) 2053
Csp6I GTAC 5 cut(s) 739, 797, 958, 1735, 1846
CspAI ACCGGT 1 cut(s) 128
CspCI CAANNNNNGTGG 2 cut(s) 1319, 1354
CviAII CATG 5 cut(s) 571, 893, 1273, 1882, 1998
CviQI GTAC 5 cut(s) 739, 797, 958, 1735, 1846
DraI TTTAAA 1 cut(s) 1648
DraIII CACNNNGTG 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 1638
Eam1104I CTCTTC 1 cut(s) 2124
EarI CTCTTC 1 cut(s) 2124
EciI GGCGGA 2 cut(s) 1736, 2114
Eco130I CCWWGG 4 cut(s) 1389, 1707, 1758, 2016
Eco147I AGGCCT 1 cut(s) 882
Eco24I GRGCYC 3 cut(s) 233, 1379, 2114
Eco31I GGTCTC 2 cut(s) 1280, 2131
Eco47I GGWCC 1 cut(s) 2011
Eco57I CTGAAG 1 cut(s) 2105
Eco81I CCTNAGG 2 cut(s) 87, 333
Eco88I CYCGRG 4 cut(s) 1246, 2081, 2107, 2206
Eco91I GGTNACC 1 cut(s) 1438
EcoNI CCTNNNNNAGG 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 1438
EcoRI GAATTC 1 cut(s) 1451
EcoRII CCWGG 2 cut(s) 1455, 2053
EcoT14I CCWWGG 4 cut(s) 1389, 1707, 1758, 2016
EcoT22I ATGCAT 1 cut(s) 547
EcoT38I GRGCYC 3 cut(s) 233, 1379, 2114
ErhI CCWWGG 4 cut(s) 1389, 1707, 1758, 2016
FaeI CATG 5 cut(s) 574, 896, 1276, 1885, 2001
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 1 cut(s) 2012
FatI CATG 5 cut(s) 570, 892, 1272, 1881, 1997
FauI CCCGC 2 cut(s) 152, 1420
FauNDI CATATG 1 cut(s) 812
FbaI TGATCA 5 cut(s) 3, 403, 768, 1092, 1494
FokI GGATG 3 cut(s) 1178, 1277, 1634
FriOI GRGCYC 3 cut(s) 233, 1379, 2114
FspBI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2306
GlaI GCGC 3 cut(s) 153, 155, 747
GsuI CTGGAG 4 cut(s) 1218, 1478, 2161, 2167
HaeIII GGCC 9 cut(s) 100, 339, 817, 882, 1640, 1829, 2021, 2079, 2288
HgaI GACGC 3 cut(s) 259, 1433, 1661
HhaI GCGC 3 cut(s) 154, 156, 748
Hin1II CATG 5 cut(s) 574, 896, 1276, 1885, 2001
Hin6I GCGC 3 cut(s) 152, 154, 746
HinP1I GCGC 3 cut(s) 152, 154, 746
HincII GTYRAC 3 cut(s) 134, 258, 2140
HindII GTYRAC 3 cut(s) 134, 258, 2140
HinfI GANTC 9 cut(s) 430, 639, 789, 1119, 1317, 1616, 1712, 1763, 2174
HpaI GTTAAC 1 cut(s) 258
HphI GGTGA 5 cut(s) 763, 1450, 1747, 1771, 1948
Hpy166II GTNNAC 8 cut(s) 134, 258, 358, 667, 823, 1846, 2060, 2140
Hpy188III TCNNGA 9 cut(s) 447, 629, 643, 859, 1235, 1273, 1729, 1774, 2128
Hpy8I GTNNAC 8 cut(s) 134, 258, 358, 667, 823, 1846, 2060, 2140
Hpy99I CGWCG 2 cut(s) 275, 1814
HpyAV CCTTC 9 cut(s) 62, 247, 256, 358, 872, 1228, 1336, 1819, 2262
HpyCH4III ACNGT 7 cut(s) 487, 743, 938, 1555, 1843, 2064, 2320
HpyCH4IV ACGT 4 cut(s) 837, 1542, 1809, 2025
HpySE526I ACGT 4 cut(s) 837, 1542, 1809, 2025
Hsp92II CATG 5 cut(s) 574, 896, 1276, 1885, 2001
HspAI GCGC 3 cut(s) 152, 154, 746
Ksp22I TGATCA 5 cut(s) 3, 403, 768, 1092, 1494
KspAI GTTAAC 1 cut(s) 258
LguI GCTCTTC 1 cut(s) 2124
LmnI GCTCC 9 cut(s) 503, 1109, 1341, 2154, 2186, 2198, 2204, 2231, 2248
Lsp1109I GCAGC 7 cut(s) 62, 92, 605, 769, 794, 1118, 1498
LweI GCATC 8 cut(s) 484, 532, 584, 856, 1034, 1532, 1732, 2209
MabI ACCWGGT 1 cut(s) 2053
MaeI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2306
MaeII ACGT 4 cut(s) 837, 1542, 1809, 2025
MaeIII GTNAC 6 cut(s) 423, 1438, 1480, 1747, 1954, 2314
MbiI CCGCTC 1 cut(s) 2149
MboII GAAGA 9 cut(s) 232, 503, 682, 722, 1312, 1702, 2111, 2176, 2179
MflI RGATCY 1 cut(s) 1991
MhlI GDGCHC 6 cut(s) 233, 1253, 1379, 1677, 2062, 2114
MlsI TGGCCA 1 cut(s) 1640
MluCI AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2335
MluNI TGGCCA 1 cut(s) 1640
MlyI GAGTC 4 cut(s) 424, 1326, 1625, 2168
MmeI TCCRAC 5 cut(s) 253, 369, 1354, 1599, 2226
Mox20I TGGCCA 1 cut(s) 1640
Mph1103I ATGCAT 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 1868
MscI TGGCCA 1 cut(s) 1640
MseI TTAA 6 cut(s) 125, 257, 413, 1350, 1647, 2338
MslI CAYNNNNRTG 2 cut(s) 1631, 1841
Msp20I TGGCCA 1 cut(s) 1640
MspA1I CMGCKG 2 cut(s) 401, 953
MspR9I CCNGG 8 cut(s) 179, 1128, 1457, 1968, 2055, 2114, 2154, 2190
MvaI CCWGG 2 cut(s) 1457, 2055
MvnI CGCG 9 cut(s) 154, 156, 197, 375, 448, 1040, 1427, 1655, 1832
NciI CCSGG 6 cut(s) 179, 1128, 1968, 2114, 2154, 2190
NdeI CATATG 1 cut(s) 812
NlaIII CATG 5 cut(s) 574, 896, 1276, 1885, 2001
NlaIV GGNNCC 8 cut(s) 99, 1387, 2013, 2080, 2194, 2200, 2227, 2287
NmuCI GTSAC 3 cut(s) 423, 1438, 1954
NruI TCGCGA 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 547
NspI RCATGY 1 cut(s) 896
OliI CACNNNNGTG 1 cut(s) 1631
PaeI GCATGC 1 cut(s) 896
PagI TCATGA 1 cut(s) 1272
PaqCI CACCTGC 1 cut(s) 2248
PauI GCGCGC 1 cut(s) 152
PceI AGGCCT 1 cut(s) 882
PciSI GCTCTTC 1 cut(s) 2124
PcsI WCGNNNNNNNCGW 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 1868
PfeI GAWTC 5 cut(s) 639, 789, 1119, 1712, 1763
PflFI GACNNNGTC 1 cut(s) 453
PfoI TCCNGGA 2 cut(s) 177, 1455
PinAI ACCGGT 1 cut(s) 128
Ple19I CGATCG 1 cut(s) 1770
PleI GAGTC 4 cut(s) 424, 1325, 1624, 2168
PpsI GAGTC 4 cut(s) 424, 1325, 1624, 2168
Ppu21I YACGTR 1 cut(s) 838
PshAI GACNNNNGTC 1 cut(s) 1808
PsiI TTATAA 1 cut(s) 930
Psp6I CCWGG 2 cut(s) 1455, 2053
PspEI GGTNACC 1 cut(s) 1438
PspGI CCWGG 2 cut(s) 1455, 2053
PspN4I GGNNCC 8 cut(s) 99, 1387, 2013, 2080, 2194, 2200, 2227, 2287
PspPI GGNCC 5 cut(s) 98, 337, 2011, 2078, 2286
PstI CTGCAG 2 cut(s) 255, 966
PstNI CAGNNNCTG 2 cut(s) 1484, 2295
PsuI RGATCY 1 cut(s) 1991
PsyI GACNNNGTC 1 cut(s) 453
PteI GCGCGC 1 cut(s) 152
PvuI CGATCG 1 cut(s) 1770
PvuII CAGCTG 2 cut(s) 401, 953
RruI TCGCGA 1 cut(s) 448
RsaI GTAC 5 cut(s) 740, 798, 959, 1736, 1847
RsaNI GTAC 5 cut(s) 739, 797, 958, 1735, 1846
RseI CAYNNNNRTG 2 cut(s) 1631, 1841
SapI GCTCTTC 1 cut(s) 2124
SaqAI TTAA 6 cut(s) 125, 257, 413, 1350, 1647, 2338
Sau96I GGNCC 5 cut(s) 98, 337, 2011, 2078, 2286
SchI GAGTC 4 cut(s) 424, 1326, 1625, 2168
ScrFI CCNGG 8 cut(s) 179, 1128, 1457, 1968, 2055, 2114, 2154, 2190
SduI GDGCHC 6 cut(s) 233, 1253, 1379, 1677, 2062, 2114
SexAI ACCWGGT 1 cut(s) 2053
SfaNI GCATC 8 cut(s) 484, 532, 584, 856, 1034, 1532, 1732, 2209
SfcI CTRYAG 4 cut(s) 251, 934, 962, 1020
SinI GGWCC 1 cut(s) 2011
SmiMI CAYNNNNRTG 2 cut(s) 1631, 1841
SmlI CTYRAG 2 cut(s) 1567, 1929
SmoI CTYRAG 2 cut(s) 1567, 1929
SphI GCATGC 1 cut(s) 896
Sse9I AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2335
SseBI AGGCCT 1 cut(s) 882
SsiI CCGC 8 cut(s) 156, 159, 375, 1427, 1721, 1830, 2099, 2147
SspMI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2306
StuI AGGCCT 1 cut(s) 882
StyD4I CCNGG 8 cut(s) 177, 1126, 1455, 1966, 2053, 2112, 2152, 2188
StyI CCWWGG 4 cut(s) 1389, 1707, 1758, 2016
TaaI ACNGT 7 cut(s) 487, 743, 938, 1555, 1843, 2064, 2320
TaiI ACGT 4 cut(s) 840, 1545, 1812, 2028
TaqII GACCGA 1 cut(s) 1022
TasI AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2335
TatI WGTACW 1 cut(s) 738
TauI GCSGC 3 cut(s) 159, 378, 1832
TfiI GAWTC 5 cut(s) 639, 789, 1119, 1712, 1763
Tru1I TTAA 6 cut(s) 125, 257, 413, 1350, 1647, 2338
Tru9I TTAA 6 cut(s) 125, 257, 413, 1350, 1647, 2338
TscAI CASTG 7 cut(s) 432, 748, 1846, 1859, 1915, 2008, 2067
TseFI GTSAC 3 cut(s) 423, 1438, 1954
TseI GCWGC 7 cut(s) 50, 80, 593, 757, 782, 1131, 1511
Tsp45I GTSAC 3 cut(s) 423, 1438, 1954
TspDTI ATGAA 7 cut(s) 1261, 1464, 1780, 1838, 1911, 2202, 2274
TspGWI ACGGA 3 cut(s) 1398, 1621, 2024
TspRI CASTG 7 cut(s) 432, 748, 1846, 1859, 1915, 2008, 2067
Tth111I GACNNNGTC 1 cut(s) 453
VneI GTGCAC 1 cut(s) 2058
VpaK11BI GGWCC 1 cut(s) 2011
XagI CCTNNNNNAGG 1 cut(s) 1077
XapI RAATTY 4 cut(s) 518, 599, 1451, 1488
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 2 cut(s) 519, 1714
XmnI GAANNNNTTC 1 cut(s) 1868
XspI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2306
Zsp2I ATGCAT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.