MD15G1155300.v1.1

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013180
Physical Location & Seq
Reverse (-)
11558278 .. 11562185
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1155300.v1.1.491

Sequence Viewer

Length: 2322 bp
ATGATAAGGTGTTTGCTTTTCATTTCCTTGTTGATCCACTCGTCTTTAGCTGCAAAACCTCCAAATGCAGGACAGAATGCAGCACCTCAGGCTCAGGGGCCTCCCCCGAAAAAATGGCAGACTTTAACCGGTCAACAGCCTCTTGTAATAGCGCGTGGCGGGCTTTCAGGGCTGTTCCCGGAAGGCAGCATCCCCGCGAGCGATATGGCAAAGACTCTTAGCTTGCCGGGCTCCGCCTTCTTCTGCAATCTTCAGTTATCGAAAGACAACGTGGGAATATGCCTGTCGAACATTCTGCTTGATAATACAACAACTATATCAATGTTCTTCCCTAAGGGCCAGAATACTTACAATGTGAACGGGAAGGATATTCGCGGCTGGTTTGCGTTGGATTTCACAGCTGATGAGTTGTTAGCCAACGTGTCGCTGACTCAAAACATTTATTCGCGACCAAGTCAGTTCGATGGTATGATGCCAATCTCTACTGTCGAAGATGTTTTGGGAACTAAACCGAATAAATTCTGGTTGAACGTGCCGTACGATTCATTTTACAACGAGCACAAGATCAGCATGGTATCATTTGTCGAGAAGGCAGTCAAATTCGCACGGATACCTTACATCTCATCTCCCGAGATCGGATTCTTGAAAGCCATTCGTGGAAAGGTGAACAAAATGAAGACAAAGCTCATCTTCCAGTTCCTTTACCCCGAAGAAACAGAACCGACAACCAAGGAAAAGTACAGCGCACTGGTGAAGCAGCTCGGAATGATCAAGTCAGTTGCAGCTGGAATCCTTGTCCCGAAAGAATACATATGGCCTGTAAACAAAAACAATTACTTGGAGGCTGCCACGACGCTTGTGAATGATGCTCACAAAGAAGGGCTAGAAGTGCATGCTTCCGGTTTTGCCAATGACGTTATCCTCCCTTATAACTACAGTTTCGATCCCACATCTGAGTACCTGCAGTTTGTCGATAACTCTCAGTTTTCGGTTGATGGTGTGCTTACAGATTTCCCTCCTACAGCATCAGAAGCCATCGCGTGCTTTACGCAGGACAAGAACGGTACGAAGCCTAAGAAAGGATTTCCTTTGATCATAACTAGAAATGGAGCTAGTGGAATCTATCCCGGCAGCACAGACCTTGCTTACAAGCAGGCAGTAGAAGATGGGGCTGACATTATCGATTGCTCGGTTCAAATGTCAAGCGATGGAGTAGCCTTCTGCTTAGACACTATAGACCTCAACTCGGGCACTAACGCGTTGAGCCAATTCATGACCAAGAGTGATACTATCCCCGAACTTCAAGCAGAAGCCGGAGTCTTCTCCTTCCAGCTCTCGTGGAGCGATATTCAGAGCTTGAAACCACAAATACAGAGCCCCTACGGAACCAAAGAAAAAATAGTCAGAAACCCCGCTAACAAGGACGCGGGCAAACTGGTGACCCTCAAAGAATTCCTGGAGTTTGCAAAGAAAAATGCAGCTACTGGAATTTTGATCAACATTGAGAATGCTGCATACCTAGCATCAAAGGCAGGCCTAGACATTGTGGGAACTGTATCCTCTGCCTTGAGCAATGCCACACTCGACAAGCAATCCACTCAGCAAGTGTTGATCAAATCGGATGACACCTCCGTGCTGTCCAAGTTTAAAGACTCAAAAAACTACAAACGAGTGTTCAACATCAAGGAGAAGATAAGCGATGCGCCCAAGGAGTCTGTAGCAGAGATCAAGAAGTACGCAGATGCAGTAATCGTCACCAAGGATTCGATCGTTAAGATCACTGATTACTTCATTTCTGGCACGACCAACGTTGTCAAGGAAATGAAGGCTGCAAACATCTCCGTGTATGCCCACTGGATGAAGAACGAGTTTGTCAGTCTCATGTTTGATTATTTCTCGGACCCCATGACGGAGATTGCTTCATACGCTCAAGGGTTCCAAGTTGATGGCATTGTCACCGATTTCCCCGGAACTGCCAGCAAGTATATGAGATCCCCGTGCACTGATATGGACCCCAAGGCCAACGTCCCATATGCAATCTTGCCAGTTGAACCAGGTTCACTGTCGAGCCTGATCGCCCCCGAGGCATTGTCTCCGGCACAAGCACCAAGCCAGGCGCTTCAAACCTCGAAGGTGGTTGACCCACCACTCCCGGAAGTGGTGAAGAAGAAGGACTCCAGCTCCGGCCCCAGCTCCGACTTGGGGAAATCCAAATCACCACCTGCTCCTTCACCTTCTGATGAAAAGTCAAGTGCTTTTGCAAACGGGGCCAGTTGGTGTGCTTCTCTAGTTGCCATGATGGTGCTTTTGCAGGCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0016020 GO:0031224 GO:0031225 GO:0044425 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GDPD

Protein Analysis

774

Amino Acids

83.91

Weight (kDa)

5.96

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 54 - 339 3.3e-08 Glycerophosphoryl diester phosphodiesterase family
GDPD PF03009 370 - 658 4.1e-22 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58050 AT5G58170
fragaria_vesca FvH4_1g01240
malus_domestica MD02G1010300.v1.1 MD15G1155300.v1.1
prunus_persica Prupe.7G259800_v2.0.a1 Prupe.7G259800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086011
rosa_laevigata RLG00000015734
rosa_multiflora Rmu_ssc0000355.1_g000023
rosa_roxburghii Rroxscaffold_2G00154850
rosa_rugosa Rorug01G0465000 Rorug01G0465100
rosa_samantha Rh2AG014300 Rh2BG014800 Rh2CG015400 Rh2DG016700
rosa_wichuraiana Rw2G001270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
AarI CACCTGC 1 cut(s) 2230
Acc36I ACCTGC 2 cut(s) 969, 2230
AccII CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
AciI CCGC 6 cut(s) 159, 195, 234, 375, 1413, 1427
AclI AACGTT 1 cut(s) 1809
AclWI GGATC 3 cut(s) 28, 938, 1986
AcsI RAATTY 4 cut(s) 518, 599, 1451, 1488
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 5 cut(s) 539, 740, 959, 1066, 1736
AfiI CCNNNNNNNGG 7 cut(s) 68, 635, 1079, 1246, 1909, 2158, 2202
AflIII ACRYGT 2 cut(s) 420, 1257
AgeI ACCGGT 1 cut(s) 128
AgsI TTSAA 8 cut(s) 529, 646, 1196, 1304, 1360, 1678, 2051, 2123
AjnI CCWGG 3 cut(s) 1455, 2053, 2112
AleI CACNNNNGTG 1 cut(s) 1631
Alw21I GWGCWC 2 cut(s) 561, 2003
Alw26I GTCTC 2 cut(s) 1883, 2097
Alw44I GTGCAC 1 cut(s) 1999
AlwI GGATC 3 cut(s) 28, 938, 1986
AlwNI CAGNNNCTG 1 cut(s) 1484
Ama87I CYCGRG 3 cut(s) 629, 1246, 2081
AoxI GGCC 7 cut(s) 98, 337, 815, 1534, 2019, 2185, 2268
ApaLI GTGCAC 1 cut(s) 1999
ApoI RAATTY 4 cut(s) 518, 599, 1451, 1488
AsiGI ACCGGT 1 cut(s) 128
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 4 cut(s) 154, 746, 1705, 2119
AspS9I GGNCC 6 cut(s) 98, 337, 1900, 2011, 2186, 2268
AsuC2I CCSGG 5 cut(s) 179, 228, 1128, 1968, 2153
AsuHPI GGTGA 8 cut(s) 676, 763, 1450, 1747, 1948, 2173, 2208, 2223
AvaI CYCGRG 3 cut(s) 629, 1246, 2081
AvaII GGWCC 2 cut(s) 1900, 2011
AxyI CCTNAGG 2 cut(s) 87, 333
BaeGI GKGCMC 2 cut(s) 1253, 2003
BanII GRGCYC 2 cut(s) 233, 1379
BauI CACGAG 1 cut(s) 1336
BbsI GAAGAC 2 cut(s) 683, 1312
Bbv12I GWGCWC 2 cut(s) 561, 2003
BccI CCATC 7 cut(s) 458, 989, 1043, 1160, 1202, 1940, 2293
BceAI ACGGC 1 cut(s) 520
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 3 cut(s) 1457, 2055, 2114
BciVI GTATCC 2 cut(s) 603, 1567
BclI TGATCA 4 cut(s) 768, 1092, 1494, 1611
BcnI CCSGG 5 cut(s) 179, 228, 1128, 1968, 2153
BcoDI GTCTC 2 cut(s) 1883, 2097
BfaI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2288
BfmI CTRYAG 5 cut(s) 934, 962, 1020, 1233, 1716
BfoI RGCGCY 1 cut(s) 2120
BfuAI ACCTGC 2 cut(s) 969, 2230
BfuI GTATCC 2 cut(s) 603, 1567
BglI GCCNNNNNGGC 1 cut(s) 2084
Bme1390I CCNGG 8 cut(s) 179, 228, 1128, 1457, 1968, 2055, 2114, 2153
Bme18I GGWCC 2 cut(s) 1900, 2011
BmeT110I CYCGRG 3 cut(s) 629, 1246, 2081
BmgT120I GGNCC 6 cut(s) 98, 337, 1900, 2011, 2186, 2268
BmiI GGNNCC 8 cut(s) 99, 232, 1387, 1902, 1937, 2013, 2188, 2269
BmrFI CCNGG 8 cut(s) 179, 228, 1128, 1457, 1968, 2055, 2114, 2153
BmsI GCATC 7 cut(s) 198, 462, 856, 1034, 1532, 1690, 1732
BpiI GAAGAC 2 cut(s) 683, 1312
BpmI CTGGAG 2 cut(s) 1478, 2161
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 1588, 1914
BpuMI CCSGG 5 cut(s) 179, 228, 1128, 1968, 2153
Bsa29I ATCGAT 1 cut(s) 1182
BsaBI GATNNNNATC 1 cut(s) 476
BsaJI CCNNGG 6 cut(s) 729, 1707, 1758, 1966, 2016, 2082
BsaWI WCCGGW 2 cut(s) 128, 899
BsaXI ACNNNNNCTCC 2 cut(s) 2165, 2195
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 635, 1079, 1246, 1909, 2158, 2202
Bse118I RCCGGY 1 cut(s) 128
Bse1I ACTGG 7 cut(s) 694, 753, 1440, 1489, 1859, 2045, 2271
Bse21I CCTNAGG 2 cut(s) 87, 333
Bse3DI GCAATG 1 cut(s) 1579
Bse8I GATNNNNATC 1 cut(s) 476
BseBI CCWGG 3 cut(s) 1457, 2055, 2114
BseCI ATCGAT 1 cut(s) 1182
BseDI CCNNGG 6 cut(s) 729, 1707, 1758, 1966, 2016, 2082
BseGI GGATG 3 cut(s) 189, 1627, 1863
BseJI GATNNNNATC 1 cut(s) 476
BseLI CCNNNNNNNGG 7 cut(s) 68, 635, 1079, 1246, 1909, 2158, 2202
BseMI GCAATG 1 cut(s) 1579
BseMII CTCAG 5 cut(s) 101, 107, 945, 995, 1613
BseNI ACTGG 7 cut(s) 694, 753, 1440, 1489, 1859, 2045, 2271
BseSI GKGCMC 2 cut(s) 1253, 2003
BseYI CCCAGC 1 cut(s) 2189
Bsh1236I CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
Bsh1285I CGRYCG 1 cut(s) 1770
BshFI GGCC 7 cut(s) 100, 339, 817, 1536, 2021, 2187, 2270
BshTI ACCGGT 1 cut(s) 128
BshVI ATCGAT 1 cut(s) 1182
BsiEI CGRYCG 1 cut(s) 1770
BsiHKAI GWGCWC 2 cut(s) 561, 2003
BsiHKCI CYCGRG 3 cut(s) 629, 1246, 2081
BsiWI CGTACG 1 cut(s) 537
BslFI GGGAC 2 cut(s) 782, 2012
BslI CCNNNNNNNGG 7 cut(s) 68, 635, 1079, 1246, 1909, 2158, 2202
BsmAI GTCTC 2 cut(s) 1883, 2097
BsmFI GGGAC 2 cut(s) 782, 2012
BsmI GAATGC 2 cut(s) 82, 1513
BsnI GGCC 7 cut(s) 100, 339, 817, 1536, 2021, 2187, 2270
BsoBI CYCGRG 3 cut(s) 629, 1246, 2081
Bsp1286I GDGCHC 5 cut(s) 233, 561, 1253, 1379, 2003
Bsp68I TCGCGA 1 cut(s) 448
BspACI CCGC 6 cut(s) 159, 195, 234, 375, 1413, 1427
BspANI GGCC 7 cut(s) 100, 339, 817, 1536, 2021, 2187, 2270
BspCNI CTCAG 5 cut(s) 100, 106, 946, 994, 1612
BspDI ATCGAT 1 cut(s) 1182
BspFNI CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
BspHI TCATGA 1 cut(s) 1272
BspLI GGNNCC 8 cut(s) 99, 232, 1387, 1902, 1937, 2013, 2188, 2269
BspMAI CTGCAG 1 cut(s) 966
BspMI ACCTGC 2 cut(s) 969, 2230
BspPI GGATC 3 cut(s) 28, 938, 1986
BsrDI GCAATG 1 cut(s) 1579
BsrFI RCCGGY 1 cut(s) 128
BsrI ACTGG 7 cut(s) 694, 753, 1440, 1489, 1859, 2045, 2271
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 6 cut(s) 729, 1707, 1758, 1966, 2016, 2082
BssSI CACGAG 1 cut(s) 1336
BssT1I CCWWGG 4 cut(s) 729, 1707, 1758, 2016
Bst2BI CACGAG 1 cut(s) 1336
Bst2UI CCWGG 3 cut(s) 1457, 2055, 2114
Bst4CI ACNGT 5 cut(s) 487, 938, 1064, 1555, 2064
BstDEI CTNAG 9 cut(s) 87, 93, 218, 333, 954, 981, 1074, 1225, 1599
BstEII GGTNACC 1 cut(s) 1438
BstENI CCTNNNNNAGG 1 cut(s) 1077
BstF5I GGATG 3 cut(s) 189, 1627, 1863
BstFNI CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
BstH2I RGCGCY 1 cut(s) 2120
BstHHI GCGC 4 cut(s) 154, 746, 1705, 2119
BstMAI GTCTC 2 cut(s) 1883, 2097
BstMCI CGRYCG 1 cut(s) 1770
BstNI CCWGG 3 cut(s) 1457, 2055, 2114
BstNSI RCATGY 1 cut(s) 896
BstPI GGTNACC 1 cut(s) 1438
BstSCI CCNGG 8 cut(s) 177, 226, 1126, 1455, 1966, 2053, 2112, 2151
BstSFI CTRYAG 5 cut(s) 934, 962, 1020, 1233, 1716
BstSLI GKGCMC 2 cut(s) 1253, 2003
BstUI CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
BstV2I GAAGAC 2 cut(s) 683, 1312
BstX2I RGATCY 1 cut(s) 1991
BstXI CCANNNNNNTGG 1 cut(s) 1946
BstYI RGATCY 1 cut(s) 1991
Bsu15I ATCGAT 1 cut(s) 1182
Bsu36I CCTNAGG 2 cut(s) 87, 333
BsuI GTATCC 2 cut(s) 603, 1567
BsuRI GGCC 7 cut(s) 100, 339, 817, 1536, 2021, 2187, 2270
BsuTUI ATCGAT 1 cut(s) 1182
BtgZI GCGATG 3 cut(s) 1021, 1221, 1713
BtsCI GGATG 3 cut(s) 189, 1627, 1863
BtsIMutI CAGTG 5 cut(s) 746, 1779, 1852, 2001, 2060
BtuMI TCGCGA 1 cut(s) 448
BveI ACCTGC 2 cut(s) 969, 2230
CaiI CAGNNNCTG 1 cut(s) 1484
CciI TCATGA 1 cut(s) 1272
CfoI GCGC 4 cut(s) 154, 746, 1705, 2119
Cfr10I RCCGGY 1 cut(s) 128
Cfr13I GGNCC 6 cut(s) 98, 337, 1900, 2011, 2186, 2268
ClaI ATCGAT 1 cut(s) 1182
CseI GACGC 2 cut(s) 862, 1433
CsiI ACCWGGT 1 cut(s) 2053
Csp6I GTAC 5 cut(s) 538, 739, 958, 1065, 1735
CspAI ACCGGT 1 cut(s) 128
CspCI CAANNNNNGTGG 2 cut(s) 838, 873
CviAII CATG 6 cut(s) 571, 893, 1273, 1882, 1906, 2296
CviQI GTAC 5 cut(s) 538, 739, 958, 1065, 1735
DdeI CTNAG 9 cut(s) 87, 93, 218, 333, 954, 981, 1074, 1225, 1599
DraI TTTAAA 1 cut(s) 1648
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 4 cut(s) 729, 1707, 1758, 2016
Eco147I AGGCCT 1 cut(s) 1536
Eco24I GRGCYC 2 cut(s) 233, 1379
Eco47I GGWCC 2 cut(s) 1900, 2011
Eco57I CTGAAG 1 cut(s) 236
Eco81I CCTNAGG 2 cut(s) 87, 333
Eco88I CYCGRG 3 cut(s) 629, 1246, 2081
Eco91I GGTNACC 1 cut(s) 1438
EcoNI CCTNNNNNAGG 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 98
EcoO65I GGTNACC 1 cut(s) 1438
EcoRI GAATTC 1 cut(s) 1451
EcoRII CCWGG 3 cut(s) 1455, 2053, 2112
EcoT14I CCWWGG 4 cut(s) 729, 1707, 1758, 2016
EcoT38I GRGCYC 2 cut(s) 233, 1379
ErhI CCWWGG 4 cut(s) 729, 1707, 1758, 2016
FaeI CATG 6 cut(s) 574, 896, 1276, 1885, 1909, 2299
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 2 cut(s) 782, 2012
FatI CATG 6 cut(s) 570, 892, 1272, 1881, 1905, 2295
FauI CCCGC 4 cut(s) 152, 202, 1420, 1420
FauNDI CATATG 2 cut(s) 812, 2032
FbaI TGATCA 4 cut(s) 768, 1092, 1494, 1611
FokI GGATG 3 cut(s) 176, 1634, 1870
FriOI GRGCYC 2 cut(s) 233, 1379
FspBI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2288
GlaI GCGC 4 cut(s) 153, 745, 1704, 2118
GsaI CCCAGC 1 cut(s) 2193
GsuI CTGGAG 2 cut(s) 1478, 2161
HaeII RGCGCY 1 cut(s) 2120
HaeIII GGCC 7 cut(s) 100, 339, 817, 1536, 2021, 2187, 2270
HgaI GACGC 2 cut(s) 862, 1433
HhaI GCGC 4 cut(s) 154, 746, 1705, 2119
Hin1II CATG 6 cut(s) 574, 896, 1276, 1885, 1909, 2299
Hin6I GCGC 4 cut(s) 152, 744, 1703, 2117
HinP1I GCGC 4 cut(s) 152, 744, 1703, 2117
HincII GTYRAC 2 cut(s) 134, 2140
HindII GTYRAC 2 cut(s) 134, 2140
HphI GGTGA 8 cut(s) 676, 763, 1450, 1747, 1948, 2173, 2208, 2223
Hpy166II GTNNAC 7 cut(s) 134, 358, 667, 823, 2001, 2060, 2140
Hpy188III TCNNGA 7 cut(s) 447, 586, 629, 643, 799, 1273, 1729
Hpy8I GTNNAC 7 cut(s) 134, 358, 667, 823, 2001, 2060, 2140
Hpy99I CGWCG 1 cut(s) 856
HpyCH4III ACNGT 5 cut(s) 487, 938, 1064, 1555, 2064
HpyCH4IV ACGT 6 cut(s) 270, 420, 531, 915, 1809, 2025
HpyF3I CTNAG 9 cut(s) 87, 93, 218, 333, 954, 981, 1074, 1225, 1599
HpySE526I ACGT 6 cut(s) 270, 420, 531, 915, 1809, 2025
Hsp92II CATG 6 cut(s) 574, 896, 1276, 1885, 1909, 2299
HspAI GCGC 4 cut(s) 152, 744, 1703, 2117
Ksp22I TGATCA 4 cut(s) 768, 1092, 1494, 1611
LmnI GCTCC 6 cut(s) 236, 1109, 1341, 2186, 2198, 2230
LweI GCATC 7 cut(s) 198, 462, 856, 1034, 1532, 1690, 1732
MabI ACCWGGT 1 cut(s) 2053
MaeI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2288
MaeII ACGT 6 cut(s) 270, 420, 531, 915, 1809, 2025
MaeIII GTNAC 3 cut(s) 1438, 1753, 1954
MflI RGATCY 1 cut(s) 1991
MhlI GDGCHC 5 cut(s) 233, 561, 1253, 1379, 2003
MluCI AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2317
MluI ACGCGT 1 cut(s) 1257
MlyI GAGTC 6 cut(s) 208, 424, 1326, 1646, 1721, 2168
MmeI TCCRAC 2 cut(s) 369, 2220
MroXI GAANNNNTTC 1 cut(s) 518
MseI TTAA 4 cut(s) 125, 1647, 1773, 2320
MslI CAYNNNNRTG 4 cut(s) 1631, 1841, 2006, 2300
MspA1I CMGCKG 2 cut(s) 401, 785
MspR9I CCNGG 8 cut(s) 179, 228, 1128, 1457, 1968, 2055, 2114, 2153
Mva1269I GAATGC 2 cut(s) 82, 1513
MvaI CCWGG 3 cut(s) 1457, 2055, 2114
MvnI CGCG 7 cut(s) 154, 197, 375, 448, 1040, 1259, 1427
NciI CCSGG 5 cut(s) 179, 228, 1128, 1968, 2153
NdeI CATATG 2 cut(s) 812, 2032
NlaIII CATG 6 cut(s) 574, 896, 1276, 1885, 1909, 2299
NlaIV GGNNCC 8 cut(s) 99, 232, 1387, 1902, 1937, 2013, 2188, 2269
NmuCI GTSAC 3 cut(s) 1438, 1753, 1954
NruI TCGCGA 1 cut(s) 448
NspI RCATGY 1 cut(s) 896
OliI CACNNNNGTG 1 cut(s) 1631
PaeI GCATGC 1 cut(s) 896
PagI TCATGA 1 cut(s) 1272
PaqCI CACCTGC 1 cut(s) 2230
PceI AGGCCT 1 cut(s) 1536
PcsI WCGNNNNNNNCGW 1 cut(s) 537
PctI GAATGC 2 cut(s) 82, 1513
PdmI GAANNNNTTC 1 cut(s) 518
PfeI GAWTC 5 cut(s) 542, 639, 789, 1119, 1763
Pfl23II CGTACG 1 cut(s) 537
PflFI GACNNNGTC 1 cut(s) 453
PfoI TCCNGGA 3 cut(s) 177, 1455, 2151
PinAI ACCGGT 1 cut(s) 128
Ple19I CGATCG 1 cut(s) 1770
PleI GAGTC 6 cut(s) 208, 424, 1325, 1646, 1720, 2168
PpsI GAGTC 6 cut(s) 208, 424, 1325, 1646, 1720, 2168
PsiI TTATAA 1 cut(s) 930
Psp1406I AACGTT 1 cut(s) 1809
Psp6I CCWGG 3 cut(s) 1455, 2053, 2112
PspEI GGTNACC 1 cut(s) 1438
PspFI CCCAGC 1 cut(s) 2189
PspGI CCWGG 3 cut(s) 1455, 2053, 2112
PspLI CGTACG 1 cut(s) 537
PspN4I GGNNCC 8 cut(s) 99, 232, 1387, 1902, 1937, 2013, 2188, 2269
PspPI GGNCC 6 cut(s) 98, 337, 1900, 2011, 2186, 2268
PsrI GAACNNNNNNTAC 2 cut(s) 521, 553
PstI CTGCAG 1 cut(s) 966
PstNI CAGNNNCTG 1 cut(s) 1484
PsuI RGATCY 1 cut(s) 1991
PsyI GACNNNGTC 1 cut(s) 453
PvuI CGATCG 1 cut(s) 1770
PvuII CAGCTG 2 cut(s) 401, 785
RruI TCGCGA 1 cut(s) 448
RsaI GTAC 5 cut(s) 539, 740, 959, 1066, 1736
RsaNI GTAC 5 cut(s) 538, 739, 958, 1065, 1735
RseI CAYNNNNRTG 4 cut(s) 1631, 1841, 2006, 2300
SaqAI TTAA 4 cut(s) 125, 1647, 1773, 2320
Sau96I GGNCC 6 cut(s) 98, 337, 1900, 2011, 2186, 2268
SchI GAGTC 6 cut(s) 208, 424, 1326, 1646, 1721, 2168
ScrFI CCNGG 8 cut(s) 179, 228, 1128, 1457, 1968, 2055, 2114, 2153
SduI GDGCHC 5 cut(s) 233, 561, 1253, 1379, 2003
SexAI ACCWGGT 1 cut(s) 2053
SfaNI GCATC 7 cut(s) 198, 462, 856, 1034, 1532, 1690, 1732
SfcI CTRYAG 5 cut(s) 934, 962, 1020, 1233, 1716
SinI GGWCC 2 cut(s) 1900, 2011
SmiMI CAYNNNNRTG 4 cut(s) 1631, 1841, 2006, 2300
SmlI CTYRAG 2 cut(s) 1567, 1929
SmoI CTYRAG 2 cut(s) 1567, 1929
SphI GCATGC 1 cut(s) 896
Sse9I AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2317
SseBI AGGCCT 1 cut(s) 1536
SsiI CCGC 6 cut(s) 159, 195, 234, 375, 1413, 1427
SspMI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2288
StuI AGGCCT 1 cut(s) 1536
StyD4I CCNGG 8 cut(s) 177, 226, 1126, 1455, 1966, 2053, 2112, 2151
StyI CCWWGG 4 cut(s) 729, 1707, 1758, 2016
TaaI ACNGT 5 cut(s) 487, 938, 1064, 1555, 2064
TaiI ACGT 6 cut(s) 273, 423, 534, 918, 1812, 2028
TasI AATT 7 cut(s) 518, 599, 832, 1268, 1451, 1488, 2317
TatI WGTACW 1 cut(s) 738
TauI GCSGC 1 cut(s) 378
TfiI GAWTC 5 cut(s) 542, 639, 789, 1119, 1763
Tru1I TTAA 4 cut(s) 125, 1647, 1773, 2320
Tru9I TTAA 4 cut(s) 125, 1647, 1773, 2320
TscAI CASTG 5 cut(s) 753, 1786, 1859, 2008, 2067
TseFI GTSAC 3 cut(s) 1438, 1753, 1954
Tsp45I GTSAC 3 cut(s) 1438, 1753, 1954
TspDTI ATGAA 9 cut(s) 10, 534, 689, 1261, 1780, 1838, 1874, 1911, 2256
TspGWI ACGGA 5 cut(s) 622, 1398, 1621, 1831, 1925
TspRI CASTG 5 cut(s) 753, 1786, 1859, 2008, 2067
Tth111I GACNNNGTC 1 cut(s) 453
VneI GTGCAC 1 cut(s) 1999
VpaK11BI GGWCC 2 cut(s) 1900, 2011
XagI CCTNNNNNAGG 1 cut(s) 1077
XapI RAATTY 4 cut(s) 518, 599, 1451, 1488
XceI RCATGY 1 cut(s) 896
XcmI CCANNNNNNNNNTGG 1 cut(s) 2197
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 6 cut(s) 884, 1101, 1113, 1520, 1538, 2288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.