Rroxscaffold_2G00154850

glycerophosphoryl diester phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013180
Physical Location & Seq
Forward (+)
91331519 .. 91335753
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154850.1

Sequence Viewer

Length: 2436 bp
ATGATGAGATGTTTGCTTCTCATTTCCCTGTTGATCCATTCAGCATTGGCCCAGGATGTAGGGCCGGAGGAGCCTCCTCCATTAGGCCAGAACGGAGGGCCAGATGAGCCGCCTCAAGGGAAGAAATGGCTGACATTAAATGGTAAAGAGCCCCTGATTGTAGCTCGTGGAGGGCTATCGGGGCTCTTTCCAGAATCGAGCAATTATGCTAATAGTATGGCCAAGTCCCTGAGTTTGATAGGCACAGCTCTCTACTGCAATCTACAATTAACGAAAGATAGCGTGGGATTATGCCTGTCAAACATTCTGCTTGATAATACAACAACAATATCTACGGTACACCCTAATGGCGAAAAAACTTACAATGTTAATGGGAAGGAAGTTCATGGGTGGTTCGCTTTAGATTTCACAGCAGATCAGCTGTCAAAGTTGACATTGACTCAAAACATCTTTTCCAGACCGGATCAATTTGACGATGCTTTGCCTATATCGACGGTTGAGGATGTTTTAAATAGCAAACCTGACCAGTTTTGGTTGCATGTGCCGTACGATGCATTTTATAACGAACACAAAATCAGTCCAGCGGCATTTGTACAAAGGCAAATGAAAGTTGCAGACATCAATTTCCTTTCATCTCCTGAGATTGGTTTCTTGAAAACCATCGGTGGAAAAGTGGACAAATTGAAGACCAAGCTCATCTTCGAGTTCCTAGGCCCCGATGAGATTGAACCCACAACTAAACAAAAGTACGGTGAATTGCTGAAGAATCTTGGAATGATCAAGTCACTAGCAGCTGGGATCCTTGTTCCAAAAGAATTCATATACCCTATTACCCCAAATAATTATTTGGATGCTCCCACTACTCTGGTGGCAGATGCTCACAAAGAAGGGGTGGAAGTATATGCTAAAGGCTTCGCAAACGATCAAAATATCCCTTACAACTATAGTATGGATGCAACAGCAGAAATCCAACAGTTCGTAGATAATTCTGATCAATTTTCTGTTGATGGGGTGCTTACAGATTTCCCACCTACGGCATCAGAAGCCATTGCATGCTTTTCGCATAACAAGGATGCCAAGCCTTACAAAGGAAGTCCGTTGATCATAAGTAGAAATGGAGCGAGTGGAAACTATCCTGGTTGCACAGACCTTGCTTATCAGAGAGCAGTAGACGATGGCGCTGACATAATCGATTGCTCGGTCCAAATGTCAAGTGATGGAGTTGCCTTCTGCTTAGATTCCATAGACCTCAACTCAGGCACCAATGCATTGACTCAATTTATGACCAAGAGTGAGACTATCCCTGAACTTCAACCTGAAGCTGGAGTCTTCTCGTTCCAGCTCACATGGGAGGAGATTCAGTCCTTGAAACCCCAACTGCAAAGTCCCCACGGACCCAAGGGAGACTTGTATAGAAACCCAGCTAACAAGGATGCAGGCAAACTGGTGACCCTTAATGAATTCCTAGAGTTCGCCAAGAAAAAGGCAACTACTGGAATTTTGATCGACATTGAGAATGCGCCATACCTAGCATCAAAGGCCGGTCTGGACATTGTTGGTACCGTAATTTCTGCCTTGAAAAATGCCTCACTCGACAAGCAATCGACTCAACAAGTCATGATCAAATCAAATGACACTTCAGTCCTGTCCAAATTCGCTGATGAGAAAACATACAAAAGAGTGTTCAGCATTAAGGACCAAGTAGGTTCTGCACCAAAGCAATCGATTGACGAGATCAAGAAGTTTGCAGATGCAGTAACCGCCGGCAAAACTTCGGTTATTCTGTCCAACTCTTTCTTCATCTCGGGAGAAACTGGCATCATCAAGGAATTGAAGGCTGCAAATCTTTCAGTGTACATCCATTACCTGAAAAACGAATTCGTCAGTCTCATGTTTGATTATCTCTCAGACCCCATTACCGAAATAGCTACATTTGCTCAAGGATTCCATGTTGATGGAATTGTGACTGATTTCCCAGGAACTGCTAGCAAATATATGAAATGTGCATGCACTGATCTTAACCCTCAGGCCAATATCCCATATCCGATTTTGCCAATTGAACCCGGTTCTCTCCTCAGCTTGCTACCCCCTGAGAAAGTGGCACCAGCAGCAGCTCCCCTCCCAGCTCTCGAAGCTAGTAGCATAGTTGACCCACCACTTCCTCCAGTGGCACCTAAAAAGGAGACCAAGCCCGACTCAGACTCCGCGTCCGGTGACAAGCCTGCTCATGCTCCTGCACCGACCGGAAAATCAAGCGCATTGGCAAATGCAGCAAACTGGGATTTGCCTTCTGTCAGAAAAGAGAGTAAGGCAGCGCCGCCTTCCGTGGCTGCAACCACGGTCGTTGGCCGTGAAAGGACAGAAACGTCATTTCACCAACAAGAACAACCGGGTCGCCCCGCCAAGAAAACCGGTTCACCTCTCCCCACTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0016020 GO:0031224 GO:0031225 GO:0044425 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GDPD

Protein Analysis

811

Amino Acids

87.55

Weight (kDa)

5.2

Isoelectric Point (pI)

39.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDPD PF03009 373 - 661 1.5e-25 Glycerophosphoryl diester phosphodiesterase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58050 AT5G58170
fragaria_vesca FvH4_1g01240
malus_domestica MD02G1010300.v1.1 MD15G1155300.v1.1
prunus_persica Prupe.7G259800_v2.0.a1 Prupe.7G259800_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0086011
rosa_laevigata RLG00000015734
rosa_multiflora Rmu_ssc0000355.1_g000023
rosa_roxburghii Rroxscaffold_2G00154850
rosa_rugosa Rorug01G0465000 Rorug01G0465100
rosa_samantha Rh2AG014300 Rh2BG014800 Rh2CG015400 Rh2DG016700
rosa_wichuraiana Rw2G001270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
AasI GACNNNNNNGTC 2 cut(s) 1640, 2365
Acc65I GGTACC 1 cut(s) 1559
AccB1I GGYRCC 4 cut(s) 1259, 1559, 2101, 2170
AccI GTMKAC 1 cut(s) 1170
AccII CGCG 1 cut(s) 2207
AciI CCGC 6 cut(s) 110, 584, 1761, 2205, 2318, 2400
AclWI GGATC 4 cut(s) 28, 471, 793, 806
AcoI YGGCCR 2 cut(s) 219, 2347
AcsI RAATTY 5 cut(s) 815, 1460, 1497, 1652, 1877
AcuI CTGAAG 3 cut(s) 782, 1338, 1623
AfaI GTAC 6 cut(s) 339, 548, 594, 749, 1561, 1856
AfiI CCNNNNNNNGG 6 cut(s) 83, 116, 644, 1033, 1088, 1322
AgeI ACCGGT 1 cut(s) 2411
AgsI TTSAA 8 cut(s) 655, 685, 728, 1313, 1369, 1579, 1834, 2060
AhdI GACNNNNNGTC 1 cut(s) 2206
AjnI CCWGG 3 cut(s) 51, 1135, 1975
AjuI GAANNNNNNNTTGG 4 cut(s) 683, 715, 1781, 1813
Alw26I GTCTC 4 cut(s) 1289, 1398, 1892, 2177
AlwI GGATC 4 cut(s) 28, 471, 793, 806
AlwNI CAGNNNCTG 1 cut(s) 1982
Ama87I CYCGRG 1 cut(s) 1804
AoxI GGCC 9 cut(s) 48, 62, 85, 98, 219, 712, 1539, 2028, 2347
ApeKI GCWGC 7 cut(s) 791, 1838, 2108, 2111, 2270, 2312, 2330
ApoI RAATTY 5 cut(s) 815, 1460, 1497, 1652, 1877
AsiGI ACCGGT 1 cut(s) 2411
Asp718I GGTACC 1 cut(s) 1559
AspA2I CCTAGG 1 cut(s) 709
AspLEI GCGC 4 cut(s) 1181, 1522, 2258, 2317
AspS9I GGNCC 7 cut(s) 49, 62, 98, 713, 1201, 1394, 1696
AsuC2I CCSGG 2 cut(s) 2064, 2391
AsuHPI GGTGA 5 cut(s) 764, 1459, 2225, 2366, 2409
AsuNHI GCTAGC 1 cut(s) 1985
AvaI CYCGRG 1 cut(s) 1804
AvaII GGWCC 3 cut(s) 1201, 1394, 1696
AvrII CCTAGG 1 cut(s) 709
AxyI CCTNAGG 1 cut(s) 2025
BalI TGGCCA 1 cut(s) 221
BamHI GGATCC 1 cut(s) 798
BanI GGYRCC 4 cut(s) 1259, 1559, 2101, 2170
BanII GRGCYC 2 cut(s) 153, 186
BauI CACGAG 1 cut(s) 165
BbsI GAAGAC 2 cut(s) 692, 1321
BbvCI CCTCAGC 1 cut(s) 2075
BbvI GCAGC 7 cut(s) 803, 1825, 2120, 2123, 2282, 2317, 2324
BccI CCATC 5 cut(s) 668, 1001, 1169, 1211, 1949
BceAI ACGGC 3 cut(s) 529, 1050, 2334
BcgI CGANNNNNNTGC 2 cut(s) 1041, 1075
BciT130I CCWGG 3 cut(s) 53, 1137, 1977
BclI TGATCA 4 cut(s) 777, 991, 1101, 1620
BcnI CCSGG 2 cut(s) 2064, 2391
BcoDI GTCTC 4 cut(s) 1289, 1398, 1892, 2177
BfaI CTAG 6 cut(s) 710, 788, 1466, 1529, 1986, 2136
BfmI CTRYAG 1 cut(s) 943
BfoI RGCGCY 2 cut(s) 1182, 2318
BlnI CCTAGG 1 cut(s) 709
Bme1390I CCNGG 5 cut(s) 53, 1137, 1977, 2064, 2391
Bme18I GGWCC 3 cut(s) 1201, 1394, 1696
BmeRI GACNNNNNGTC 1 cut(s) 2206
BmeT110I CYCGRG 1 cut(s) 1804
BmgT120I GGNCC 7 cut(s) 49, 62, 98, 713, 1201, 1394, 1696
BmiI GGNNCC 8 cut(s) 72, 715, 800, 1261, 1396, 1561, 2103, 2172
BmrFI CCNGG 5 cut(s) 53, 1137, 1977, 2064, 2391
BmrI ACTGGG 1 cut(s) 2287
BmtI GCTAGC 1 cut(s) 1989
BmuI ACTGGG 1 cut(s) 2287
BpiI GAAGAC 2 cut(s) 692, 1321
BpmI CTGGAG 2 cut(s) 1344, 2148
Bpu10I CCTNAGC 1 cut(s) 2075
BpuEI CTTGAG 2 cut(s) 99, 1923
BpuMI CCSGG 2 cut(s) 2064, 2391
Bsa29I ATCGAT 2 cut(s) 1191, 1724
BsaI GGTCTC 1 cut(s) 2177
BsaJI CCNNGG 7 cut(s) 51, 709, 1390, 1398, 1975, 2325, 2337
BsaWI WCCGGW 4 cut(s) 460, 2210, 2243, 2411
BsaXI ACNNNNNCTCC 4 cut(s) 1346, 1376, 2186, 2216
Bsc4I CCNNNNNNNGG 6 cut(s) 83, 116, 644, 1033, 1088, 1322
Bse118I RCCGGY 3 cut(s) 1541, 1763, 2411
Bse1I ACTGG 6 cut(s) 526, 1449, 1498, 1819, 2165, 2282
Bse21I CCTNAGG 1 cut(s) 2025
Bse3DI GCAATG 1 cut(s) 1047
BseBI CCWGG 3 cut(s) 53, 1137, 1977
BseCI ATCGAT 2 cut(s) 1191, 1724
BseDI CCNNGG 7 cut(s) 51, 709, 1390, 1398, 1975, 2325, 2337
BseGI GGATG 7 cut(s) 61, 508, 856, 958, 1078, 1438, 1857
BseLI CCNNNNNNNGG 6 cut(s) 83, 116, 644, 1033, 1088, 1322
BseMI GCAATG 1 cut(s) 1047
BseMII CTCAG 8 cut(s) 221, 630, 1269, 1920, 2039, 2082, 2089, 2211
BseNI ACTGG 6 cut(s) 526, 1449, 1498, 1819, 2165, 2282
BseRI GAGGAG 4 cut(s) 66, 83, 1367, 2063
BseXI GCAGC 7 cut(s) 803, 1825, 2120, 2123, 2282, 2317, 2324
BseYI CCCAGC 3 cut(s) 794, 1420, 2122
BsgI GTGCAG 2 cut(s) 1695, 2220
Bsh1236I CGCG 1 cut(s) 2207
Bsh1285I CGRYCG 2 cut(s) 2244, 2343
BshFI GGCC 9 cut(s) 50, 64, 87, 100, 221, 714, 1541, 2030, 2349
BshNI GGYRCC 4 cut(s) 1259, 1559, 2101, 2170
BshTI ACCGGT 1 cut(s) 2411
BshVI ATCGAT 2 cut(s) 1191, 1724
BsiEI CGRYCG 2 cut(s) 2244, 2343
BsiHKCI CYCGRG 1 cut(s) 1804
BsiSI CCGG 9 cut(s) 65, 461, 1542, 1764, 2064, 2211, 2244, 2390, 2412
BsiWI CGTACG 1 cut(s) 546
BslFI GGGAC 2 cut(s) 211, 1371
BslI CCNNNNNNNGG 6 cut(s) 83, 116, 644, 1033, 1088, 1322
BsmAI GTCTC 4 cut(s) 1289, 1398, 1892, 2177
BsmFI GGGAC 2 cut(s) 211, 1371
BsmI GAATGC 1 cut(s) 1522
BsnI GGCC 9 cut(s) 50, 64, 87, 100, 221, 714, 1541, 2030, 2349
Bso31I GGTCTC 1 cut(s) 2177
BsoBI CYCGRG 1 cut(s) 1804
Bsp1286I GDGCHC 2 cut(s) 153, 186
Bsp1407I TGTACA 2 cut(s) 592, 1854
BspACI CCGC 6 cut(s) 110, 584, 1761, 2205, 2318, 2400
BspANI GGCC 9 cut(s) 50, 64, 87, 100, 221, 714, 1541, 2030, 2349
BspCNI CTCAG 8 cut(s) 222, 631, 1268, 1919, 2038, 2083, 2088, 2210
BspDI ATCGAT 2 cut(s) 1191, 1724
BspFNI CGCG 1 cut(s) 2207
BspHI TCATGA 1 cut(s) 1617
BspLI GGNNCC 8 cut(s) 72, 715, 800, 1261, 1396, 1561, 2103, 2172
BspOI GCTAGC 1 cut(s) 1989
BspPI GGATC 4 cut(s) 28, 471, 793, 806
BspT107I GGYRCC 4 cut(s) 1259, 1559, 2101, 2170
BspTNI GGTCTC 1 cut(s) 2177
BsrDI GCAATG 1 cut(s) 1047
BsrFI RCCGGY 3 cut(s) 1541, 1763, 2411
BsrGI TGTACA 2 cut(s) 592, 1854
BsrI ACTGG 6 cut(s) 526, 1449, 1498, 1819, 2165, 2282
BssAI RCCGGY 3 cut(s) 1541, 1763, 2411
BssECI CCNNGG 7 cut(s) 51, 709, 1390, 1398, 1975, 2325, 2337
BssSI CACGAG 1 cut(s) 165
BssT1I CCWWGG 2 cut(s) 709, 1398
Bst2BI CACGAG 1 cut(s) 165
Bst2UI CCWGG 3 cut(s) 53, 1137, 1977
Bst4CI ACNGT 7 cut(s) 337, 496, 752, 975, 1564, 2341, 2431
BstAUI TGTACA 2 cut(s) 592, 1854
BstC8I GCNNGC 7 cut(s) 1054, 1438, 1765, 1987, 2008, 2081, 2223
BstDEI CTNAG 9 cut(s) 230, 639, 1234, 1255, 1906, 2025, 2075, 2091, 2197
BstDSI CCRYGG 3 cut(s) 1390, 2325, 2337
BstEII GGTNACC 1 cut(s) 1447
BstENI CCTNNNNNAGG 2 cut(s) 81, 1086
BstF5I GGATG 7 cut(s) 61, 508, 856, 958, 1078, 1438, 1857
BstFNI CGCG 1 cut(s) 2207
BstH2I RGCGCY 2 cut(s) 1182, 2318
BstHHI GCGC 4 cut(s) 1181, 1522, 2258, 2317
BstMAI GTCTC 4 cut(s) 1289, 1398, 1892, 2177
BstMCI CGRYCG 2 cut(s) 2244, 2343
BstNI CCWGG 3 cut(s) 53, 1137, 1977
BstNSI RCATGY 3 cut(s) 542, 1056, 2010
BstPI GGTNACC 1 cut(s) 1447
BstSCI CCNGG 5 cut(s) 51, 1135, 1975, 2062, 2389
BstSFI CTRYAG 1 cut(s) 943
BstUI CGCG 1 cut(s) 2207
BstV1I GCAGC 7 cut(s) 803, 1825, 2120, 2123, 2282, 2317, 2324
BstV2I GAAGAC 2 cut(s) 692, 1321
BstX2I RGATCY 1 cut(s) 798
BstXI CCANNNNNNTGG 2 cut(s) 865, 1955
BstYI RGATCY 1 cut(s) 798
Bsu15I ATCGAT 2 cut(s) 1191, 1724
Bsu36I CCTNAGG 1 cut(s) 2025
BsuRI GGCC 9 cut(s) 50, 64, 87, 100, 221, 714, 1541, 2030, 2349
BsuTUI ATCGAT 2 cut(s) 1191, 1724
BtgI CCRYGG 3 cut(s) 1390, 2325, 2337
BtsCI GGATG 7 cut(s) 61, 508, 856, 958, 1078, 1438, 1857
BtsIMutI CAGTG 4 cut(s) 1857, 2010, 2172, 2427
Cac8I GCNNGC 7 cut(s) 1054, 1438, 1765, 1987, 2008, 2081, 2223
CaiI CAGNNNCTG 1 cut(s) 1982
CciI TCATGA 1 cut(s) 1617
CfoI GCGC 4 cut(s) 1181, 1522, 2258, 2317
Cfr10I RCCGGY 3 cut(s) 1541, 1763, 2411
Cfr13I GGNCC 7 cut(s) 49, 62, 98, 713, 1201, 1394, 1696
ClaI ATCGAT 2 cut(s) 1191, 1724
CseI GACGC 1 cut(s) 2196
Csp6I GTAC 6 cut(s) 338, 547, 593, 748, 1560, 1855
CspAI ACCGGT 1 cut(s) 2411
CspCI CAANNNNNGTGG 2 cut(s) 2326, 2361
CviAII CATG 9 cut(s) 386, 539, 1053, 1347, 1618, 1891, 1949, 2007, 2228
CviQI GTAC 6 cut(s) 338, 547, 593, 748, 1560, 1855
DdeI CTNAG 9 cut(s) 230, 639, 1234, 1255, 1906, 2025, 2075, 2091, 2197
DraI TTTAAA 1 cut(s) 510
DrdI GACNNNNNNGTC 2 cut(s) 1640, 2365
DriI GACNNNNNGTC 1 cut(s) 2206
DseDI GACNNNNNNGTC 2 cut(s) 1640, 2365
EaeI YGGCCR 2 cut(s) 219, 2347
Eam1105I GACNNNNNGTC 1 cut(s) 2206
Eco130I CCWWGG 2 cut(s) 709, 1398
Eco24I GRGCYC 2 cut(s) 153, 186
Eco31I GGTCTC 1 cut(s) 2177
Eco47I GGWCC 3 cut(s) 1201, 1394, 1696
Eco57I CTGAAG 3 cut(s) 782, 1338, 1623
Eco81I CCTNAGG 1 cut(s) 2025
Eco88I CYCGRG 1 cut(s) 1804
Eco91I GGTNACC 1 cut(s) 1447
EcoNI CCTNNNNNAGG 2 cut(s) 81, 1086
EcoO109I RGGNCCY 1 cut(s) 713
EcoO65I GGTNACC 1 cut(s) 1447
EcoRI GAATTC 3 cut(s) 815, 1460, 1877
EcoRII CCWGG 3 cut(s) 51, 1135, 1975
EcoT14I CCWWGG 2 cut(s) 709, 1398
EcoT22I ATGCAT 2 cut(s) 556, 1270
EcoT38I GRGCYC 2 cut(s) 153, 186
ErhI CCWWGG 2 cut(s) 709, 1398
FaeI CATG 9 cut(s) 389, 542, 1056, 1350, 1621, 1894, 1952, 2010, 2231
FalI AAGNNNNNCTT 4 cut(s) 683, 715, 1391, 1423
FaqI GGGAC 2 cut(s) 211, 1371
FatI CATG 9 cut(s) 385, 538, 1052, 1346, 1617, 1890, 1948, 2006, 2227
FauI CCCGC 1 cut(s) 2407
FbaI TGATCA 4 cut(s) 777, 991, 1101, 1620
FblI GTMKAC 1 cut(s) 1170
FokI GGATG 7 cut(s) 68, 515, 863, 965, 1085, 1445, 1844
FriOI GRGCYC 2 cut(s) 153, 186
FspBI CTAG 6 cut(s) 710, 788, 1466, 1529, 1986, 2136
GlaI GCGC 4 cut(s) 1180, 1521, 2257, 2316
GsaI CCCAGC 3 cut(s) 798, 1424, 2126
GsuI CTGGAG 2 cut(s) 1344, 2148
HaeII RGCGCY 2 cut(s) 1182, 2318
HaeIII GGCC 9 cut(s) 50, 64, 87, 100, 221, 714, 1541, 2030, 2349
HapII CCGG 9 cut(s) 65, 461, 1542, 1764, 2064, 2211, 2244, 2390, 2412
HgaI GACGC 1 cut(s) 2196
HhaI GCGC 4 cut(s) 1181, 1522, 2258, 2317
Hin1II CATG 9 cut(s) 389, 542, 1056, 1350, 1621, 1894, 1952, 2010, 2231
Hin6I GCGC 4 cut(s) 1179, 1520, 2256, 2315
HinP1I GCGC 4 cut(s) 1179, 1520, 2256, 2315
HincII GTYRAC 2 cut(s) 432, 2149
HindII GTYRAC 2 cut(s) 432, 2149
HpaII CCGG 9 cut(s) 65, 461, 1542, 1764, 2064, 2211, 2244, 2390, 2412
HphI GGTGA 5 cut(s) 764, 1459, 2225, 2366, 2409
Hpy166II GTNNAC 7 cut(s) 340, 432, 676, 1171, 1855, 2149, 2417
Hpy188I TCNGA 8 cut(s) 991, 1042, 1161, 1909, 2046, 2200, 2297, 2435
Hpy188III TCNNGA 9 cut(s) 191, 456, 638, 652, 1547, 1618, 1738, 1806, 2129
Hpy8I GTNNAC 7 cut(s) 340, 432, 676, 1171, 1855, 2149, 2417
Hpy99I CGWCG 1 cut(s) 496
HpyAV CCTTC 6 cut(s) 370, 881, 1237, 1828, 2298, 2331
HpyCH4III ACNGT 7 cut(s) 337, 496, 752, 975, 1564, 2341, 2431
HpyCH4IV ACGT 1 cut(s) 2366
HpyF3I CTNAG 9 cut(s) 230, 639, 1234, 1255, 1906, 2025, 2075, 2091, 2197
HpySE526I ACGT 1 cut(s) 2366
Hsp92II CATG 9 cut(s) 389, 542, 1056, 1350, 1621, 1894, 1952, 2010, 2231
HspAI GCGC 4 cut(s) 1179, 1520, 2256, 2315
KpnI GGTACC 1 cut(s) 1563
KroI GCCGGC 1 cut(s) 1763
KroNI GCCGGC 1 cut(s) 1765
Ksp22I TGATCA 4 cut(s) 777, 991, 1101, 1620
LmnI GCTCC 5 cut(s) 70, 859, 1118, 2119, 2236
Lsp1109I GCAGC 7 cut(s) 803, 1825, 2120, 2123, 2282, 2317, 2324
MaeI CTAG 6 cut(s) 710, 788, 1466, 1529, 1986, 2136
MaeII ACGT 1 cut(s) 2366
MaeIII GTNAC 5 cut(s) 783, 1447, 1756, 1963, 2213
MboII GAAGA 6 cut(s) 133, 691, 697, 775, 1321, 1789
MfeI CAATTG 1 cut(s) 2055
MflI RGATCY 1 cut(s) 798
MhlI GDGCHC 2 cut(s) 153, 186
MlsI TGGCCA 1 cut(s) 221
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 6 cut(s) 433, 1267, 1335, 1600, 2189, 2195
MmeI TCCRAC 2 cut(s) 994, 1812
Mox20I TGGCCA 1 cut(s) 221
Mph1103I ATGCAT 2 cut(s) 556, 1270
MroNI GCCGGC 1 cut(s) 1763
MscI TGGCCA 1 cut(s) 221
MseI TTAA 7 cut(s) 137, 269, 369, 509, 1455, 1692, 2019
MslI CAYNNNNRTG 2 cut(s) 345, 1953
Msp20I TGGCCA 1 cut(s) 221
MspA1I CMGCKG 3 cut(s) 421, 584, 794
MspI CCGG 9 cut(s) 65, 461, 1542, 1764, 2064, 2211, 2244, 2390, 2412
MspR9I CCNGG 5 cut(s) 53, 1137, 1977, 2064, 2391
MunI CAATTG 1 cut(s) 2055
Mva1269I GAATGC 1 cut(s) 1522
MvaI CCWGG 3 cut(s) 53, 1137, 1977
MvnI CGCG 1 cut(s) 2207
NaeI GCCGGC 1 cut(s) 1765
NciI CCSGG 2 cut(s) 2064, 2391
NgoMIV GCCGGC 1 cut(s) 1763
NheI GCTAGC 1 cut(s) 1985
NlaIII CATG 9 cut(s) 389, 542, 1056, 1350, 1621, 1894, 1952, 2010, 2231
NlaIV GGNNCC 8 cut(s) 72, 715, 800, 1261, 1396, 1561, 2103, 2172
NmuCI GTSAC 4 cut(s) 783, 1447, 1963, 2213
NsiI ATGCAT 2 cut(s) 556, 1270
NspI RCATGY 3 cut(s) 542, 1056, 2010
PaeI GCATGC 2 cut(s) 1056, 2010
PagI TCATGA 1 cut(s) 1617
PctI GAATGC 1 cut(s) 1522
PdiI GCCGGC 1 cut(s) 1765
PfeI GAWTC 5 cut(s) 194, 766, 1238, 1357, 1944
Pfl23II CGTACG 1 cut(s) 546
PinAI ACCGGT 1 cut(s) 2411
PleI GAGTC 6 cut(s) 433, 1267, 1334, 1600, 2189, 2195
PpsI GAGTC 6 cut(s) 433, 1267, 1334, 1600, 2189, 2195
PsiI TTATAA 1 cut(s) 561
Psp6I CCWGG 3 cut(s) 51, 1135, 1975
PspEI GGTNACC 1 cut(s) 1447
PspFI CCCAGC 3 cut(s) 794, 1420, 2122
PspGI CCWGG 3 cut(s) 51, 1135, 1975
PspLI CGTACG 1 cut(s) 546
PspN4I GGNNCC 8 cut(s) 72, 715, 800, 1261, 1396, 1561, 2103, 2172
PspPI GGNCC 7 cut(s) 49, 62, 98, 713, 1201, 1394, 1696
PstNI CAGNNNCTG 1 cut(s) 1982
PsuI RGATCY 1 cut(s) 798
PvuII CAGCTG 2 cut(s) 421, 794
RsaI GTAC 6 cut(s) 339, 548, 594, 749, 1561, 1856
RsaNI GTAC 6 cut(s) 338, 547, 593, 748, 1560, 1855
RseI CAYNNNNRTG 2 cut(s) 345, 1953
SaqAI TTAA 7 cut(s) 137, 269, 369, 509, 1455, 1692, 2019
Sau96I GGNCC 7 cut(s) 49, 62, 98, 713, 1201, 1394, 1696
SchI GAGTC 6 cut(s) 433, 1267, 1335, 1600, 2189, 2195
ScrFI CCNGG 5 cut(s) 53, 1137, 1977, 2064, 2391
SduI GDGCHC 2 cut(s) 153, 186
SfcI CTRYAG 1 cut(s) 943
SinI GGWCC 3 cut(s) 1201, 1394, 1696
SmiMI CAYNNNNRTG 2 cut(s) 345, 1953
SmlI CTYRAG 2 cut(s) 114, 1938
SmoI CTYRAG 2 cut(s) 114, 1938
SphI GCATGC 2 cut(s) 1056, 2010
SsiI CCGC 6 cut(s) 110, 584, 1761, 2205, 2318, 2400
SspMI CTAG 6 cut(s) 710, 788, 1466, 1529, 1986, 2136
StyD4I CCNGG 5 cut(s) 51, 1135, 1975, 2062, 2389
StyI CCWWGG 2 cut(s) 709, 1398
TaaI ACNGT 7 cut(s) 337, 496, 752, 975, 1564, 2341, 2431
TaiI ACGT 1 cut(s) 2369
TaqI TCGA 9 cut(s) 197, 491, 702, 1191, 1506, 1593, 1604, 1724, 2130
TaqII GACCGA 1 cut(s) 1189
TatI WGTACW 2 cut(s) 592, 1854
TauI GCSGC 3 cut(s) 112, 587, 2320
TfiI GAWTC 5 cut(s) 194, 766, 1238, 1357, 1944
Tru1I TTAA 7 cut(s) 137, 269, 369, 509, 1455, 1692, 2019
Tru9I TTAA 7 cut(s) 137, 269, 369, 509, 1455, 1692, 2019
TscAI CASTG 4 cut(s) 1857, 2017, 2172, 2434
TseFI GTSAC 4 cut(s) 783, 1447, 1963, 2213
TseI GCWGC 7 cut(s) 791, 1838, 2108, 2111, 2270, 2312, 2330
Tsp45I GTSAC 4 cut(s) 783, 1447, 1963, 2213
TspDTI ATGAA 7 cut(s) 374, 620, 621, 808, 1473, 1789, 2012
TspGWI ACGGA 4 cut(s) 108, 1086, 1407, 2314
TspRI CASTG 4 cut(s) 1857, 2017, 2172, 2434
VpaK11BI GGWCC 3 cut(s) 1201, 1394, 1696
XagI CCTNNNNNAGG 2 cut(s) 81, 1086
XapI RAATTY 5 cut(s) 815, 1460, 1497, 1652, 1877
XceI RCATGY 3 cut(s) 542, 1056, 2010
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XmaJI CCTAGG 1 cut(s) 709
XmiI GTMKAC 1 cut(s) 1170
XspI CTAG 6 cut(s) 710, 788, 1466, 1529, 1986, 2136
Zsp2I ATGCAT 2 cut(s) 556, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.