AT5G62030

Diphthamide synthesis DPH2 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010480
Physical Location & Seq
Forward (+)
24918479 .. 24920955
2477 bp
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UTR
Exon/CDS
Intron
AT5G62030.1

Sequence Viewer

Length: 1362 bp
ATGGAGCTTTCTGATCCAAACAATGGCAAGACCAACAAACCAAAGCAACCTCCAAAACGTTTCATCAAGAACCAAATCCCTGATTCAATCCTCGGTGATGCATCCCTCAACGCTGCTATCTCCATCTTACCTTCAATTTACCATTTTGAAGTCCATAAATGTGTGTGGCGTATCAAAACATCAAACGCTAAACGCATAGCTCTGCAGCTTCCAGAGGGTCTTCTTATGTACGCTCTTACACTCTCTGATATCTTCACATCTTTTGCTGGAGCTTCTCACTGTTTTGTACTCGGTGATGTTACTTATGGAGCTTGTTGTGTTGATGATTTCTCTGCTTGTGCGCTTGGTGCTGACTTGCTTATTCACTATGGACATAGTTGTCTTGTCCCTATCGACTCTACAAAAATCCCTTGCCTTTATGTTTTTGTTGAGATCCAGATAGATGTCAAATGCTTGTTGAACACTATACATCTGAATCTCGCGAGTGATGTTAAGAACATCATTCTTGCAGGCACGATTCAGTTCACTTCGGCGATTAGAGCTGTGAAACCAGAGTTGGAGAAGCAAGGGTTCAATGTTTTGATCCCTCAGTCAAAGCCTCTATCTGCTGGTGAAGTCCTTGGCTGTACAGCTCCCAAGGTGAAGACGGTTGAGGATTGTAAAGACCAAGTACTGGTCTTTGTAGCTGATGGGAGATTTCATCTAGAAGCATTCATGATAGCCAATCCAAAAATCAAGGCGTTTAGGTATGATCCATATCTTGGCAAGCTGTTTTTAGAGGAGTATGATCATAAGGGAATGAGGGAGACGAGAATGAGAGCGATTGCACGGGCCAGGGAGGCTAAAACTTGGGGGATCGTGTTGGGGACATTGGGAAGGCAAGGAAATCCTAAGATTCTTGAGAGATTAGAGAAGAAAATGATGGAGAAAGGGATAGATAGCACAGTTGTTTTAATGTCGGAACTTAGTCCCACTAGAGTGGCATTGTTTGAAGACTCGGTGGATGCTTGGGTCCAAATAGCATGCCCAAGACTTTCTATTGATTGGGGTGAAGCGTTTCTTAAGCCGCTTCTGACGACTTTTGAGGCTGAGATTGCTCTAGGATTCATCCGCGGCTGGTGGGAGAATGATTCTTCTAGTAGAGTAAATTCTTCATCTGGCTGTTGCAAAGAGGACAAAGAAACAAGCTGTGCGTGTAAAAATGAGAACGTGAAAGATGACAAGAAAGACAATGATGGGGCACTCGATGGAGACTATCCAATGGATTACTATGCACAAGAAGGTGGAGAATGGAACTCTTCATATCTCAAGAAGTCATCTCGTCCCATTCGAAGAAACCCTTTACCTTCTTCTGTAGTTTAG

Protein Analysis

453

Amino Acids

50.34

Weight (kDa)

6.72

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 70 - 374 5.3e-111 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 378
AccB7I CCANNNNNTGG 3 cut(s) 23, 673, 761
AccII CGCG 2 cut(s) 482, 1113
AciI CCGC 3 cut(s) 1067, 1111, 1113
AclI AACGTT 1 cut(s) 58
AclWI GGATC 5 cut(s) 8, 427, 577, 746, 863
AcsI RAATTY 1 cut(s) 1147
AfaI GTAC 4 cut(s) 230, 288, 628, 672
AfiI CCNNNNNNNGG 3 cut(s) 23, 673, 761
AflII CTTAAG 1 cut(s) 1061
AgsI TTSAA 6 cut(s) 87, 135, 149, 460, 574, 992
AjnI CCWGG 1 cut(s) 833
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AleI CACNNNNGTG 1 cut(s) 977
Alw26I GTCTC 2 cut(s) 800, 1245
AlwI GGATC 5 cut(s) 8, 427, 577, 746, 863
AoxI GGCC 1 cut(s) 831
ApeKI GCWGC 2 cut(s) 113, 205
ApoI RAATTY 1 cut(s) 1147
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 831, 1012
AsuHPI GGTGA 5 cut(s) 107, 305, 623, 652, 1061
AsuII TTCGAA 1 cut(s) 1330
AvaII GGWCC 1 cut(s) 1012
BaeGI GKGCMC 1 cut(s) 1243
BbsI GAAGAC 3 cut(s) 212, 650, 999
BbvI GCAGC 2 cut(s) 100, 217
BccI CCATC 5 cut(s) 131, 683, 916, 1229, 1241
BciT130I CCWGG 1 cut(s) 835
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 800, 1245
BfaI CTAG 4 cut(s) 704, 975, 1100, 1137
BfmI CTRYAG 2 cut(s) 203, 1353
BfrI CTTAAG 1 cut(s) 1061
BglI GCCNNNNNGGC 1 cut(s) 839
BisI GCNGC 4 cut(s) 114, 206, 1067, 1114
BlsI GCNGC 4 cut(s) 115, 207, 1068, 1115
BmcAI AGTACT 1 cut(s) 672
Bme1390I CCNGG 1 cut(s) 835
Bme18I GGWCC 1 cut(s) 1012
BmgT120I GGNCC 2 cut(s) 831, 1012
BmiI GGNNCC 1 cut(s) 1013
BmrFI CCNGG 1 cut(s) 835
BmsI GCATC 3 cut(s) 88, 110, 994
BpiI GAAGAC 3 cut(s) 212, 650, 999
BpmI CTGGAG 1 cut(s) 288
Bpu14I TTCGAA 1 cut(s) 1330
BpuEI CTTGAG 2 cut(s) 920, 1292
BsaBI GATNNNNATC 1 cut(s) 756
BsaJI CCNNGG 5 cut(s) 91, 619, 636, 834, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 673, 761
Bse1I ACTGG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 756
BseBI CCWGG 1 cut(s) 835
BseDI CCNNGG 5 cut(s) 91, 619, 636, 834, 1111
BseGI GGATG 3 cut(s) 101, 1009, 1107
BseJI GATNNNNATC 1 cut(s) 756
BseLI CCNNNNNNNGG 3 cut(s) 23, 673, 761
BseMII CTCAG 2 cut(s) 602, 1080
BseNI ACTGG 1 cut(s) 678
BseRI GAGGAG 1 cut(s) 794
BseSI GKGCMC 1 cut(s) 1243
BseXI GCAGC 2 cut(s) 100, 217
Bsh1236I CGCG 2 cut(s) 482, 1113
BshFI GGCC 1 cut(s) 833
BslFI GGGAC 4 cut(s) 371, 880, 954, 1308
BslI CCNNNNNNNGG 3 cut(s) 23, 673, 761
BsmAI GTCTC 2 cut(s) 800, 1245
BsmBI CGTCTC 1 cut(s) 800
BsmFI GGGAC 4 cut(s) 371, 880, 954, 1308
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 1 cut(s) 833
Bsp119I TTCGAA 1 cut(s) 1330
Bsp1286I GDGCHC 1 cut(s) 1243
Bsp1407I TGTACA 1 cut(s) 626
Bsp143I GATC 6 cut(s) 13, 432, 582, 751, 787, 855
Bsp68I TCGCGA 1 cut(s) 482
BspACI CCGC 3 cut(s) 1067, 1111, 1113
BspANI GGCC 1 cut(s) 833
BspCNI CTCAG 2 cut(s) 601, 1081
BspFNI CGCG 2 cut(s) 482, 1113
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 1 cut(s) 1013
BspMAI CTGCAG 1 cut(s) 207
BspPI GGATC 5 cut(s) 8, 427, 577, 746, 863
BspT104I TTCGAA 1 cut(s) 1330
BspTI CTTAAG 1 cut(s) 1061
BsrGI TGTACA 1 cut(s) 626
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 5 cut(s) 91, 619, 636, 834, 1111
BssMI GATC 6 cut(s) 13, 432, 582, 751, 787, 855
BssT1I CCWWGG 2 cut(s) 619, 636
Bst2UI CCWGG 1 cut(s) 835
Bst4CI ACNGT 3 cut(s) 281, 649, 946
Bst6I CTCTTC 1 cut(s) 1303
BstAFI CTTAAG 1 cut(s) 1061
BstAUI TGTACA 1 cut(s) 626
BstBI TTCGAA 1 cut(s) 1330
BstC8I GCNNGC 3 cut(s) 511, 767, 1024
BstDEI CTNAG 4 cut(s) 588, 891, 965, 1089
BstDSI CCRYGG 1 cut(s) 1111
BstF5I GGATG 3 cut(s) 101, 1009, 1107
BstFNI CGCG 2 cut(s) 482, 1113
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 6 cut(s) 16, 435, 585, 754, 790, 858
BstMAI GTCTC 2 cut(s) 800, 1245
BstMBI GATC 6 cut(s) 13, 432, 582, 751, 787, 855
BstMWI GCNNNNNNNGC 4 cut(s) 347, 539, 839, 1094
BstNI CCWGG 1 cut(s) 835
BstNSI RCATGY 1 cut(s) 1026
BstSCI CCNGG 1 cut(s) 833
BstSFI CTRYAG 2 cut(s) 203, 1353
BstSLI GKGCMC 1 cut(s) 1243
BstUI CGCG 2 cut(s) 482, 1113
BstV1I GCAGC 2 cut(s) 100, 217
BstV2I GAAGAC 3 cut(s) 212, 650, 999
BstX2I RGATCY 1 cut(s) 432
BstXI CCANNNNNNTGG 1 cut(s) 979
BstYI RGATCY 1 cut(s) 432
BsuRI GGCC 1 cut(s) 833
BtgI CCRYGG 1 cut(s) 1111
BtsCI GGATG 3 cut(s) 101, 1009, 1107
BtsIMutI CAGTG 1 cut(s) 277
BtuMI TCGCGA 1 cut(s) 482
Cac8I GCNNGC 3 cut(s) 511, 767, 1024
CciI TCATGA 1 cut(s) 714
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 831, 1012
Cfr42I CCGCGG 1 cut(s) 1114
Csp6I GTAC 4 cut(s) 229, 287, 627, 671
CviAII CATG 2 cut(s) 715, 1023
CviQI GTAC 4 cut(s) 229, 287, 627, 671
DdeI CTNAG 4 cut(s) 588, 891, 965, 1089
DpnI GATC 6 cut(s) 15, 434, 584, 753, 789, 857
DpnII GATC 6 cut(s) 13, 432, 582, 751, 787, 855
DrdI GACNNNNNNGTC 1 cut(s) 378
DseDI GACNNNNNNGTC 1 cut(s) 378
Eam1104I CTCTTC 1 cut(s) 1303
EarI CTCTTC 1 cut(s) 1303
Eco130I CCWWGG 2 cut(s) 619, 636
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 1012
EcoRII CCWGG 1 cut(s) 833
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 2 cut(s) 619, 636
EcoT22I ATGCAT 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 619, 636
Esp3I CGTCTC 1 cut(s) 800
FaeI CATG 2 cut(s) 718, 1026
FalI AAGNNNNNCTT 4 cut(s) 1044, 1076, 1324, 1356
FaqI GGGAC 4 cut(s) 371, 880, 954, 1308
FatI CATG 2 cut(s) 714, 1022
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 114, 206, 1067, 1114
FokI GGATG 3 cut(s) 88, 1016, 1094
Fsp4HI GCNGC 4 cut(s) 114, 206, 1067, 1114
FspBI CTAG 4 cut(s) 704, 975, 1100, 1137
GlaI GCGC 1 cut(s) 342
GluI GCNGC 4 cut(s) 114, 206, 1067, 1114
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 1 cut(s) 833
HhaI GCGC 1 cut(s) 343
Hin1II CATG 2 cut(s) 718, 1026
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HinfI GANTC 8 cut(s) 83, 395, 475, 517, 895, 995, 1104, 1130
HphI GGTGA 5 cut(s) 107, 305, 623, 652, 1061
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 5 cut(s) 13, 247, 474, 961, 1074
Hpy188III TCNNGA 8 cut(s) 67, 212, 436, 481, 704, 715, 899, 1309
Hpy8I GTNNAC 1 cut(s) 525
HpyAV CCTTC 4 cut(s) 141, 870, 1274, 1356
HpyCH4III ACNGT 3 cut(s) 281, 649, 946
HpyCH4IV ACGT 2 cut(s) 58, 1209
HpyCH4V TGCA 6 cut(s) 101, 205, 509, 827, 1167, 1274
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 539, 839, 1094
HpyF3I CTNAG 4 cut(s) 588, 891, 965, 1089
HpySE526I ACGT 2 cut(s) 58, 1209
Hsp92II CATG 2 cut(s) 718, 1026
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 787
KspI CCGCGG 1 cut(s) 1114
Kzo9I GATC 6 cut(s) 13, 432, 582, 751, 787, 855
LmnI GCTCC 4 cut(s) 4, 269, 308, 637
Lsp1109I GCAGC 2 cut(s) 100, 217
LweI GCATC 3 cut(s) 88, 110, 994
MaeI CTAG 4 cut(s) 704, 975, 1100, 1137
MaeII ACGT 2 cut(s) 58, 1209
MaeIII GTNAC 1 cut(s) 298
MalI GATC 6 cut(s) 15, 434, 584, 753, 789, 857
MboI GATC 6 cut(s) 13, 432, 582, 751, 787, 855
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 1 cut(s) 1243
MluCI AATT 2 cut(s) 135, 1147
MlyI GAGTC 2 cut(s) 389, 989
MmeI TCCRAC 2 cut(s) 537, 939
Mph1103I ATGCAT 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 1056
MseI TTAA 3 cut(s) 492, 953, 1062
MslI CAYNNNNRTG 2 cut(s) 159, 977
MspA1I CMGCKG 1 cut(s) 1113
MspCI CTTAAG 1 cut(s) 1061
MspR9I CCNGG 1 cut(s) 835
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 1 cut(s) 835
MvnI CGCG 2 cut(s) 482, 1113
MwoI GCNNNNNNNGC 4 cut(s) 347, 539, 839, 1094
NdeII GATC 6 cut(s) 13, 432, 582, 751, 787, 855
NlaIII CATG 2 cut(s) 718, 1026
NlaIV GGNNCC 1 cut(s) 1013
NruI TCGCGA 1 cut(s) 482
NsiI ATGCAT 1 cut(s) 103
NspI RCATGY 1 cut(s) 1026
NspV TTCGAA 1 cut(s) 1330
OliI CACNNNNGTG 1 cut(s) 977
PaeI GCATGC 1 cut(s) 1026
PagI TCATGA 1 cut(s) 714
PcsI WCGNNNNNNNCGW 1 cut(s) 1327
PctI GAATGC 1 cut(s) 710
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 6 cut(s) 83, 475, 517, 895, 1104, 1130
PflMI CCANNNNNTGG 3 cut(s) 23, 673, 761
PkrI GCNGC 4 cut(s) 115, 207, 1068, 1115
PleI GAGTC 2 cut(s) 389, 989
PpsI GAGTC 2 cut(s) 389, 989
Psp1406I AACGTT 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 833
PspGI CCWGG 1 cut(s) 833
PspN4I GGNNCC 1 cut(s) 1013
PspPI GGNCC 2 cut(s) 831, 1012
PstI CTGCAG 1 cut(s) 207
PsuI RGATCY 1 cut(s) 432
RruI TCGCGA 1 cut(s) 482
RsaI GTAC 4 cut(s) 230, 288, 628, 672
RsaNI GTAC 4 cut(s) 229, 287, 627, 671
RseI CAYNNNNRTG 2 cut(s) 159, 977
SacII CCGCGG 1 cut(s) 1114
SaqAI TTAA 3 cut(s) 492, 953, 1062
SatI GCNGC 4 cut(s) 114, 206, 1067, 1114
Sau3AI GATC 6 cut(s) 13, 432, 582, 751, 787, 855
Sau96I GGNCC 2 cut(s) 831, 1012
ScaI AGTACT 1 cut(s) 672
SchI GAGTC 2 cut(s) 389, 989
ScrFI CCNGG 1 cut(s) 835
SduI GDGCHC 1 cut(s) 1243
SfaNI GCATC 3 cut(s) 88, 110, 994
SfcI CTRYAG 2 cut(s) 203, 1353
Sfr303I CCGCGG 1 cut(s) 1114
SfuI TTCGAA 1 cut(s) 1330
SgrBI CCGCGG 1 cut(s) 1114
SinI GGWCC 1 cut(s) 1012
SmiMI CAYNNNNRTG 2 cut(s) 159, 977
SmlI CTYRAG 3 cut(s) 899, 1061, 1307
SmoI CTYRAG 3 cut(s) 899, 1061, 1307
SphI GCATGC 1 cut(s) 1026
Sse9I AATT 2 cut(s) 135, 1147
SsiI CCGC 3 cut(s) 1067, 1111, 1113
SspMI CTAG 4 cut(s) 704, 975, 1100, 1137
StyD4I CCNGG 1 cut(s) 833
StyI CCWWGG 2 cut(s) 619, 636
TaaI ACNGT 3 cut(s) 281, 649, 946
TaiI ACGT 2 cut(s) 61, 1212
TaqI TCGA 3 cut(s) 393, 1245, 1330
TasI AATT 2 cut(s) 135, 1147
TatI WGTACW 3 cut(s) 286, 626, 670
TauI GCSGC 2 cut(s) 1069, 1116
TfiI GAWTC 6 cut(s) 83, 475, 517, 895, 1104, 1130
Tru1I TTAA 3 cut(s) 492, 953, 1062
Tru9I TTAA 3 cut(s) 492, 953, 1062
TscAI CASTG 1 cut(s) 284
TseI GCWGC 2 cut(s) 113, 205
TspDTI ATGAA 6 cut(s) 52, 689, 703, 1096, 1143, 1290
TspRI CASTG 1 cut(s) 284
Van91I CCANNNNNTGG 3 cut(s) 23, 673, 761
Vha464I CTTAAG 1 cut(s) 1061
VpaK11BI GGWCC 1 cut(s) 1012
XapI RAATTY 1 cut(s) 1147
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 4 cut(s) 704, 975, 1100, 1137
ZrmI AGTACT 1 cut(s) 672
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.