MD04G1037900.v1.1

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010480
Physical Location & Seq
Reverse (-)
4219525 .. 4223501
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1037900.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGGATCCCAGCCAGGAGTCTAGTCTGTTGCCTCTCCCTGATTCTCAGCAGACAAGGAAGCCGCATGCGCCACCAAAGCGGTTTGTGAGGAACCAAATTCCGGACTCAATCCTCCACGACTCAGCTCTGAATGCTGCAATAGCCCTCCTTCCCTCCAACTACAACTTTGAAGTCCACAAGTGCGTCTGGCGTGTGCGCTCCACCAATGCCTCACGGGTGGCCCTCCAACTCCCCGAGGGCCTCCTCATGTACTCTCTGGTCCTCTCTGACATCCTCTCCACCTTCGGCGGTGTCTCCCAGTGCTTTGTTCTCGGTGACGCAACCTACGGTGCGTGCTGTGTAGACGACCTTGCTGCCAAGGCACTCGATGCTGACCTCCTCATCCACTTTGGCCACAGCTGCCTTGTCCCCCTCCACGTCACCTCAATCCCCTGTCTCTACGTCTTCGTCGAAATCTCAATTGATGTCTCCCGATTAATCCAGACCGTCCATCTCAACCTCCAAAACTCTCACTGTAAAAACCTAGTGCTTGCTGGAACAATCCAATTCGCCTCCGCAATCCGAGCGGCCAAACCTGAGCTAGAAACCCATGGTTTCAAGGTCCTCATTCCACAGTCAAAACCCTTGTCTGCCGGTGAGGTTTTGGGTTGTACTGCCCCAAAAATTTCACGCAAAATAACCAAAGGCAACGAGGAGGAGGAAGAGGAGAGCGTAGTGGTGTTTGTGGCGGACGGGCGTTTTCATTTAGAAGCGATTATGATAGCAAATCCTGGGCTGAAGACGTTTCGGTACGACCCATATATGGGGAAACTGTTTTTGGAGGAATATGATCACAAGGGGATGAGGGAGTCGAGGAAGAGCGCAATTTTGAAAGCAAAGGAAGCCTCGAATTGGGGCGTTGTGTTGGGGACTTTAGGAAGGCAAGGCAACCCCAGGATTCTACAGAGACTGGAAAAGAAAATGAAGGACGAAGGGTTTTCTTATACCGTGTTTTTGATGTCAGAGATTAGTCCTCCAAGAATTGCTCTGTTTGAAGACTCAGTGGATGCTTGGATTCAGATTGCTTGTCCTCGCTTGTCCATTGACTGGGGTGATGCGTTTACAAAGCCCCTTTTGAATCCTTTTGAGGCTGACATTGCTCTTGGCTTCATTCCTCCTTGGTGGGACAAAAATAAGAAGAGTGATTCTGGTTGTTGTGGTTGTGCATGTGCTAATGGAAATCAGGTTGGGGAAGAAGATGTTGTTGGGGATTATCCCATGGACTATTATGCTTTGGATGGAGGGGAATGGAATTCCTCTTATGTCAACAAGCCGTCCCACCCTATTCGGAGGGATTCTGCATCGGCTACTACTGCAAACACTGGATAG

Protein Analysis

456

Amino Acids

50.11

Weight (kDa)

5.87

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 77 - 388 3.1e-106 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1086
AccBSI CCGCTC 1 cut(s) 566
AccI GTMKAC 1 cut(s) 342
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 6 cut(s) 62, 79, 288, 555, 566, 728
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 391, 567
AcsI RAATTY 3 cut(s) 96, 663, 1291
AcuI CTGAAG 1 cut(s) 797
AfaI GTAC 3 cut(s) 251, 652, 791
AfiI CCNNNNNNNGG 5 cut(s) 100, 802, 803, 891, 1086
AgsI TTSAA 5 cut(s) 170, 598, 871, 1034, 1117
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 3 cut(s) 12, 769, 932
AjuI GAANNNNNNNTTGG 4 cut(s) 800, 832, 1227, 1259
AluBI AGCT 3 cut(s) 125, 399, 580
AluI AGCT 3 cut(s) 125, 399, 580
Alw26I GTCTC 4 cut(s) 298, 440, 472, 940
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 949
Ama87I CYCGRG 1 cut(s) 233
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 4 cut(s) 219, 238, 391, 567
ApeKI GCWGC 3 cut(s) 134, 353, 399
ApoI RAATTY 3 cut(s) 96, 663, 1291
ArsI GACNNNNNNTTYG 2 cut(s) 611, 643
AseI ATTAAT 1 cut(s) 476
AspLEI GCGC 3 cut(s) 70, 198, 863
AspS9I GGNCC 4 cut(s) 220, 238, 259, 601
AsuHPI GGTGA 4 cut(s) 326, 412, 647, 1103
AvaI CYCGRG 1 cut(s) 233
AvaII GGWCC 2 cut(s) 259, 601
BalI TGGCCA 1 cut(s) 393
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 3 cut(s) 436, 785, 1041
BbvI GCAGC 3 cut(s) 121, 340, 386
BccI CCATC 2 cut(s) 498, 1271
BceAI ACGGC 1 cut(s) 1297
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 3 cut(s) 14, 771, 934
BclI TGATCA 1 cut(s) 829
BcoDI GTCTC 4 cut(s) 298, 440, 472, 940
BfaI CTAG 3 cut(s) 21, 524, 581
BfmI CTRYAG 1 cut(s) 941
BisI GCNGC 5 cut(s) 62, 135, 354, 400, 567
BlsI GCNGC 5 cut(s) 63, 136, 355, 401, 568
Bme1390I CCNGG 3 cut(s) 14, 771, 934
Bme18I GGWCC 2 cut(s) 259, 601
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 4 cut(s) 220, 238, 259, 601
BmiI GGNNCC 2 cut(s) 6, 92
BmrFI CCNGG 3 cut(s) 14, 771, 934
BmrI ACTGGG 2 cut(s) 292, 1096
BmsI GCATC 4 cut(s) 358, 1036, 1084, 1349
BmuI ACTGGG 2 cut(s) 292, 1096
BpiI GAAGAC 3 cut(s) 436, 785, 1041
Bpu10I CCTNAGC 1 cut(s) 576
BsaJI CCNNGG 7 cut(s) 234, 357, 589, 770, 932, 1157, 1257
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 5 cut(s) 100, 802, 803, 891, 1086
Bse118I RCCGGY 1 cut(s) 632
Bse1I ACTGG 4 cut(s) 298, 954, 1091, 1366
Bse3DI GCAATG 1 cut(s) 1134
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 3 cut(s) 14, 771, 934
BseDI CCNNGG 7 cut(s) 234, 357, 589, 770, 932, 1157, 1257
BseGI GGATG 5 cut(s) 270, 381, 846, 1051, 1282
BseLI CCNNNNNNNGG 5 cut(s) 100, 802, 803, 891, 1086
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 4 cut(s) 59, 135, 567, 1053
BseNI ACTGG 4 cut(s) 298, 954, 1091, 1366
BseRI GAGGAG 5 cut(s) 233, 368, 707, 710, 719
BseXI GCAGC 3 cut(s) 121, 340, 386
BseYI CCCAGC 1 cut(s) 8
BshFI GGCC 4 cut(s) 221, 240, 393, 569
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 101, 633
BslFI GGGAC 4 cut(s) 392, 922, 1178, 1300
BslI CCNNNNNNNGG 5 cut(s) 100, 802, 803, 891, 1086
BsmAI GTCTC 4 cut(s) 298, 440, 472, 940
BsmFI GGGAC 4 cut(s) 392, 922, 1178, 1300
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 4 cut(s) 221, 240, 393, 569
BsoBI CYCGRG 1 cut(s) 233
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 2 cut(s) 4, 829
Bsp19I CCATGG 2 cut(s) 589, 1257
BspACI CCGC 6 cut(s) 62, 79, 288, 555, 566, 728
BspANI GGCC 4 cut(s) 221, 240, 393, 569
BspCNI CTCAG 4 cut(s) 58, 134, 568, 1052
BspEI TCCGGA 1 cut(s) 100
BspLI GGNNCC 2 cut(s) 6, 92
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 851
BsrBI CCGCTC 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 632
BsrI ACTGG 4 cut(s) 298, 954, 1091, 1366
BssAI RCCGGY 1 cut(s) 632
BssECI CCNNGG 7 cut(s) 234, 357, 589, 770, 932, 1157, 1257
BssMI GATC 2 cut(s) 4, 829
BssT1I CCWWGG 4 cut(s) 357, 589, 1157, 1257
Bst2UI CCWGG 3 cut(s) 14, 771, 934
Bst4CI ACNGT 6 cut(s) 329, 487, 515, 615, 813, 988
Bst6I CTCTTC 3 cut(s) 696, 851, 1172
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 66, 334, 531
BstDEI CTNAG 4 cut(s) 45, 121, 576, 1039
BstDSI CCRYGG 2 cut(s) 589, 1257
BstF5I GGATG 5 cut(s) 270, 381, 846, 1051, 1282
BstHHI GCGC 3 cut(s) 70, 198, 863
BstKTI GATC 2 cut(s) 7, 832
BstMAI GTCTC 4 cut(s) 298, 440, 472, 940
BstMBI GATC 2 cut(s) 4, 829
BstNI CCWGG 3 cut(s) 14, 771, 934
BstNSI RCATGY 2 cut(s) 68, 1209
BstSCI CCNGG 3 cut(s) 12, 769, 932
BstSFI CTRYAG 1 cut(s) 941
BstV1I GCAGC 3 cut(s) 121, 340, 386
BstV2I GAAGAC 3 cut(s) 436, 785, 1041
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 4 cut(s) 221, 240, 393, 569
BtgI CCRYGG 2 cut(s) 589, 1257
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 5 cut(s) 270, 381, 846, 1051, 1282
BtsIMutI CAGTG 4 cut(s) 305, 511, 1047, 1359
Cac8I GCNNGC 3 cut(s) 66, 334, 531
CaiI CAGNNNCTG 1 cut(s) 949
CfoI GCGC 3 cut(s) 70, 198, 863
Cfr10I RCCGGY 1 cut(s) 632
Cfr13I GGNCC 4 cut(s) 220, 238, 259, 601
CseI GACGC 2 cut(s) 172, 326
Csp6I GTAC 3 cut(s) 250, 651, 790
CviAII CATG 5 cut(s) 65, 247, 590, 1206, 1258
CviQI GTAC 3 cut(s) 250, 651, 790
DdeI CTNAG 4 cut(s) 45, 121, 576, 1039
DpnI GATC 2 cut(s) 6, 831
DpnII GATC 2 cut(s) 4, 829
EaeI YGGCCR 2 cut(s) 391, 567
Eam1104I CTCTTC 3 cut(s) 696, 851, 1172
EarI CTCTTC 3 cut(s) 696, 851, 1172
EciI GGCGGA 1 cut(s) 743
Eco130I CCWWGG 4 cut(s) 357, 589, 1157, 1257
Eco47I GGWCC 2 cut(s) 259, 601
Eco57I CTGAAG 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 233
EcoO109I RGGNCCY 2 cut(s) 238, 601
EcoRI GAATTC 1 cut(s) 1291
EcoRII CCWGG 3 cut(s) 12, 769, 932
EcoT14I CCWWGG 4 cut(s) 357, 589, 1157, 1257
ErhI CCWWGG 4 cut(s) 357, 589, 1157, 1257
FaeI CATG 5 cut(s) 68, 250, 593, 1209, 1261
FaqI GGGAC 4 cut(s) 392, 922, 1178, 1300
FatI CATG 5 cut(s) 64, 246, 589, 1205, 1257
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 5 cut(s) 62, 135, 354, 400, 567
FokI GGATG 5 cut(s) 257, 368, 853, 1058, 1289
Fsp4HI GCNGC 5 cut(s) 62, 135, 354, 400, 567
FspBI CTAG 3 cut(s) 21, 524, 581
GlaI GCGC 3 cut(s) 69, 197, 862
GluI GCNGC 5 cut(s) 62, 135, 354, 400, 567
GsaI CCCAGC 1 cut(s) 12
HaeIII GGCC 4 cut(s) 221, 240, 393, 569
HapII CCGG 2 cut(s) 101, 633
HgaI GACGC 2 cut(s) 172, 326
HhaI GCGC 3 cut(s) 70, 198, 863
Hin1II CATG 5 cut(s) 68, 250, 593, 1209, 1261
Hin6I GCGC 3 cut(s) 68, 196, 861
HinP1I GCGC 3 cut(s) 68, 196, 861
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HpaII CCGG 2 cut(s) 101, 633
HphI GGTGA 4 cut(s) 326, 412, 647, 1103
Hpy166II GTNNAC 4 cut(s) 175, 343, 1101, 1306
Hpy188I TCNGA 6 cut(s) 129, 268, 563, 1003, 1059, 1329
Hpy188III TCNNGA 3 cut(s) 101, 471, 481
Hpy8I GTNNAC 4 cut(s) 175, 343, 1101, 1306
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 5 cut(s) 158, 292, 912, 958, 965
HpyCH4III ACNGT 6 cut(s) 329, 487, 515, 615, 813, 988
HpyCH4IV ACGT 3 cut(s) 417, 441, 782
HpyCH4V TGCA 4 cut(s) 137, 1205, 1340, 1355
HpyF3I CTNAG 4 cut(s) 45, 121, 576, 1039
HpySE526I ACGT 3 cut(s) 417, 441, 782
Hsp92II CATG 5 cut(s) 68, 250, 593, 1209, 1261
HspAI GCGC 3 cut(s) 68, 196, 861
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 2 cut(s) 4, 829
LguI GCTCTTC 1 cut(s) 851
LmnI GCTCC 1 cut(s) 203
Lsp1109I GCAGC 3 cut(s) 121, 340, 386
LweI GCATC 4 cut(s) 358, 1036, 1084, 1349
MaeI CTAG 3 cut(s) 21, 524, 581
MaeII ACGT 3 cut(s) 417, 441, 782
MaeIII GTNAC 2 cut(s) 314, 418
MalI GATC 2 cut(s) 6, 831
MbiI CCGCTC 1 cut(s) 566
MboI GATC 2 cut(s) 4, 829
MboII GAAGA 8 cut(s) 436, 713, 790, 868, 1046, 1189, 1244, 1247
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 4
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 8 cut(s) 96, 459, 545, 663, 864, 889, 1020, 1291
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 5 cut(s) 26, 98, 113, 857, 1031
MmeI TCCRAC 2 cut(s) 180, 250
Mox20I TGGCCA 1 cut(s) 393
MroI TCCGGA 1 cut(s) 100
MscI TGGCCA 1 cut(s) 393
MseI TTAA 1 cut(s) 476
Msp20I TGGCCA 1 cut(s) 393
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 2 cut(s) 101, 633
MspR9I CCNGG 3 cut(s) 14, 771, 934
MunI CAATTG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 3 cut(s) 14, 771, 934
NcoI CCATGG 2 cut(s) 589, 1257
NdeII GATC 2 cut(s) 4, 829
NlaIII CATG 5 cut(s) 68, 250, 593, 1209, 1261
NlaIV GGNNCC 2 cut(s) 6, 92
NmuCI GTSAC 2 cut(s) 314, 418
NspI RCATGY 2 cut(s) 68, 1209
PaeI GCATGC 1 cut(s) 68
PciSI GCTCTTC 1 cut(s) 851
PcsI WCGNNNNNNNCGW 1 cut(s) 447
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 6 cut(s) 41, 937, 1054, 1117, 1184, 1334
PflMI CCANNNNNTGG 1 cut(s) 1086
PkrI GCNGC 5 cut(s) 63, 136, 355, 401, 568
PleI GAGTC 5 cut(s) 25, 98, 113, 856, 1031
PpsI GAGTC 5 cut(s) 25, 98, 113, 856, 1031
PpuMI RGGWCCY 1 cut(s) 601
PshBI ATTAAT 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 601
Psp6I CCWGG 3 cut(s) 12, 769, 932
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 3 cut(s) 12, 769, 932
PspN4I GGNNCC 2 cut(s) 6, 92
PspPI GGNCC 4 cut(s) 220, 238, 259, 601
PspPPI RGGWCCY 1 cut(s) 601
PstNI CAGNNNCTG 1 cut(s) 949
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 399
RsaI GTAC 3 cut(s) 251, 652, 791
RsaNI GTAC 3 cut(s) 250, 651, 790
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 5 cut(s) 62, 135, 354, 400, 567
Sau3AI GATC 2 cut(s) 4, 829
Sau96I GGNCC 4 cut(s) 220, 238, 259, 601
SchI GAGTC 5 cut(s) 26, 98, 113, 857, 1031
ScrFI CCNGG 3 cut(s) 14, 771, 934
SfaNI GCATC 4 cut(s) 358, 1036, 1084, 1349
SfcI CTRYAG 1 cut(s) 941
SinI GGWCC 2 cut(s) 259, 601
SphI GCATGC 1 cut(s) 68
Sse9I AATT 8 cut(s) 96, 459, 545, 663, 864, 889, 1020, 1291
SsiI CCGC 6 cut(s) 62, 79, 288, 555, 566, 728
SspMI CTAG 3 cut(s) 21, 524, 581
StyD4I CCNGG 3 cut(s) 12, 769, 932
StyI CCWWGG 4 cut(s) 357, 589, 1157, 1257
TaaI ACNGT 6 cut(s) 329, 487, 515, 615, 813, 988
TaiI ACGT 3 cut(s) 420, 444, 785
TaqI TCGA 4 cut(s) 366, 450, 851, 887
TasI AATT 8 cut(s) 96, 459, 545, 663, 864, 889, 1020, 1291
TatI WGTACW 2 cut(s) 249, 650
TauI GCSGC 2 cut(s) 64, 569
TfiI GAWTC 6 cut(s) 41, 937, 1054, 1117, 1184, 1334
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 4 cut(s) 305, 518, 1047, 1366
TseFI GTSAC 2 cut(s) 314, 418
TseI GCWGC 3 cut(s) 134, 353, 399
Tsp45I GTSAC 2 cut(s) 314, 418
TspDTI ATGAA 3 cut(s) 731, 977, 1138
TspRI CASTG 4 cut(s) 305, 518, 1047, 1366
Van91I CCANNNNNTGG 1 cut(s) 1086
VpaK11BI GGWCC 2 cut(s) 259, 601
VspI ATTAAT 1 cut(s) 476
XapI RAATTY 3 cut(s) 96, 663, 1291
XceI RCATGY 2 cut(s) 68, 1209
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 3 cut(s) 21, 524, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.