FvH4_5g30480

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010480
Physical Location & Seq
Reverse (-)
21336021 .. 21341021
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30480.t1

Sequence Viewer

Length: 1332 bp
ATGGAGGAGGCGCCAAAATCCAGTCTGGCGCTACTCGGCGACTCCGAACAGCAGCGAAAGCCACAAGCGCCGAAGCGGTTCGTCCGGAGCCAAATCCCGGACTCCATACTCCACGACCCTTCTCTCAACGCCGCCATAAGCCTCCTCCCCTCCAACTACCACTTCGAGGTCCACAAATGCGTCTGGCGCGTCCGCTCCACGCACGCCTCGCGCGTCGCCCTCCAGCTCCCGGAGGGCCTCCTCATGTACTCCCTCGTCCTCTCCGACATCCTCTCCACCTTCGGCGGCGCCTCCCAGTGCTTCGTCCTCGGCGACGCCACGTACGGCGCGTGCTGCGTCGACGACCTCGCCGCCAAGGCCCTCAACGCCGACCTCCTCATCCACTTCGGCCACAGCTGCCTCGTCCCCCTCCACGTCACCTCCATCCCCTGCCTCTACGTCTTCGTCGAAATCTCTATCGACGTCGGCCGCTTAATCCAGACCGTCCATCTCAACCTCCAAAACCCTATCCCTAAAACCCTAGTCCTCGCCGGAACAATCCAGTTCGCTTCGGCGATCCGAGCCGCCAAACCGGAGCTGGAGGCCCGGGGGTTCAAGGTCTTAGTCCCACAGTCGAAGCCGTTGTCCGCCGGCGAGGTTTTGGGCTGTACCGCCCCGAAAATCTCGCGCGGTGAGGACGGTGACGCTGTACTGGTCTTCGTCGCCGACGGCCGGTTTCATCTGGAAGCTTTTATGATAGCTAATCCGGGGGTTAAGGCGTTTCGGTACGATCCGTATATGGGGAAGCTGTTTCTGGAGGAATATGATCACAAGGGGATGAGGGAGTCGAGGAAGAGTGCAGTTTTGAGAGCCAAAGAGGCTTCGAATTGGGGCATTGTGCTCGGGACCTTAGGGAGGCAAGGGAATCCGAGGATTCTTCAGAGATTGGAGAAGAAGATGAGGGAGAAGGGGTTTAATTATACTGTGGTTTTGATGTCGGAGATTAGTCCTGCAAGAATTGCTTTGTTTGAGGACTCTGTGGATGCTTGGATACAGATTGCGTGTCCTCGGCTGTCCATTGATTGGGGGGAAGCGTTTACGAAACCCCTTTTGAACCCTTTCGAGACTGACATTGCTCTCGGTTTTGTTCCTGGTTGGTGGGAGAGGGATAAGAAAAGTGATTCAGGTTGCTGTGCTTGTGGTAATGGCAATGGGACTACTGAAGATCTTGTGGGGGATTATCCTATGGATTACTATGCTGCGGATGGAGGGGAGTGGAACTCCTCTTATGTCAACAAGCCTACACGTCCTGTTCGCAGAGATTCTGCATTGGCTAGTGCTAAAAGTGAATAG

Protein Analysis

444

Amino Acids

48.66

Weight (kDa)

6.57

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 76 - 380 2.4e-106 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AccB1I GGYRCC 2 cut(s) 10, 287
AccB7I CCANNNNNTGG 1 cut(s) 1062
AccBSI CCGCTC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 339
AccII CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
AccIII TCCGGA 1 cut(s) 84
AclWI GGATC 2 cut(s) 550, 764
AcoI YGGCCR 3 cut(s) 388, 466, 709
AcuI CTGAAG 2 cut(s) 902, 1221
AcyI GRCGYC 4 cut(s) 11, 288, 315, 462
AfaI GTAC 5 cut(s) 248, 323, 649, 690, 767
AfiI CCNNNNNNNGG 7 cut(s) 97, 166, 229, 711, 779, 894, 1062
AflIII ACRYGT 1 cut(s) 1283
AgsI TTSAA 2 cut(s) 595, 1093
AjiI CACGTC 2 cut(s) 415, 1286
AjnI CCWGG 1 cut(s) 1129
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 6 cut(s) 226, 396, 577, 728, 740, 787
AluI AGCT 6 cut(s) 226, 396, 577, 728, 740, 787
Alw21I GWGCWC 1 cut(s) 882
Alw26I GTCTC 1 cut(s) 1097
AlwI GGATC 2 cut(s) 550, 764
Ama87I CYCGRG 2 cut(s) 585, 881
Aor13HI TCCGGA 1 cut(s) 84
AoxI GGCC 6 cut(s) 235, 357, 388, 466, 582, 709
ApeKI GCWGC 4 cut(s) 52, 333, 396, 1238
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 2 cut(s) 77, 1097
AspLEI GCGC 8 cut(s) 13, 31, 70, 189, 213, 290, 329, 669
AspS9I GGNCC 5 cut(s) 169, 235, 358, 583, 885
AsuC2I CCSGG 5 cut(s) 98, 230, 586, 587, 747
AsuHPI GGTGA 3 cut(s) 409, 683, 692
AsuII TTCGAA 1 cut(s) 863
AvaI CYCGRG 2 cut(s) 585, 881
AvaII GGWCC 2 cut(s) 169, 885
AxyI CCTNAGG 1 cut(s) 889
BanI GGYRCC 2 cut(s) 10, 287
BbsI GAAGAC 2 cut(s) 433, 688
Bbv12I GWGCWC 1 cut(s) 882
BbvI GCAGC 4 cut(s) 64, 320, 383, 1225
BccI CCATC 3 cut(s) 431, 495, 1238
BceAI ACGGC 3 cut(s) 340, 604, 724
BcgI CGANNNNNNTGC 2 cut(s) 862, 896
BciT130I CCWGG 1 cut(s) 1131
BciVI GTATCC 1 cut(s) 1023
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 5 cut(s) 98, 230, 586, 587, 747
BcoDI GTCTC 1 cut(s) 1097
BfaI CTAG 2 cut(s) 521, 1314
BfoI RGCGCY 4 cut(s) 14, 32, 71, 291
BfuI GTATCC 1 cut(s) 1023
BglI GCCNNNNNGGC 2 cut(s) 356, 857
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 9 cut(s) 53, 132, 286, 334, 351, 397, 469, 564, 1239
BlsI GCNGC 9 cut(s) 54, 133, 287, 335, 352, 398, 470, 565, 1240
Bme1390I CCNGG 6 cut(s) 98, 230, 586, 587, 747, 1131
Bme18I GGWCC 2 cut(s) 169, 885
BmeT110I CYCGRG 2 cut(s) 585, 881
BmgBI CACGTC 2 cut(s) 415, 1286
BmgT120I GGNCC 5 cut(s) 169, 235, 358, 583, 885
BmiI GGNNCC 4 cut(s) 12, 89, 289, 886
BmrFI CCNGG 6 cut(s) 98, 230, 586, 587, 747, 1131
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 1012
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 2 cut(s) 433, 688
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
BpmI CTGGAG 3 cut(s) 206, 599, 815
Bpu14I TTCGAA 1 cut(s) 863
BpuMI CCSGG 5 cut(s) 98, 230, 586, 587, 747
BsaAI YACGTR 1 cut(s) 321
BsaHI GRCGYC 4 cut(s) 11, 288, 315, 462
BsaJI CCNNGG 7 cut(s) 307, 354, 585, 586, 746, 908, 1046
BsaWI WCCGGW 2 cut(s) 84, 571
BsaXI ACNNNNNCTCC 6 cut(s) 257, 287, 404, 434, 935, 965
Bsc4I CCNNNNNNNGG 7 cut(s) 97, 166, 229, 711, 779, 894, 1062
Bse118I RCCGGY 2 cut(s) 629, 711
Bse1I ACTGG 4 cut(s) 21, 295, 541, 696
Bse21I CCTNAGG 1 cut(s) 889
Bse3DI GCAATG 2 cut(s) 1110, 1195
BseAI TCCGGA 1 cut(s) 84
BseBI CCWGG 1 cut(s) 1131
BseDI CCNNGG 7 cut(s) 307, 354, 585, 586, 746, 908, 1046
BseGI GGATG 6 cut(s) 267, 378, 423, 822, 1027, 1249
BseLI CCNNNNNNNGG 7 cut(s) 97, 166, 229, 711, 779, 894, 1062
BseMI GCAATG 2 cut(s) 1110, 1195
BseNI ACTGG 4 cut(s) 21, 295, 541, 696
BseRI GAGGAG 5 cut(s) 20, 134, 230, 365, 1252
BseX3I CGGCCG 2 cut(s) 466, 709
BseXI GCAGC 4 cut(s) 64, 320, 383, 1225
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
Bsh1285I CGRYCG 2 cut(s) 469, 712
BshFI GGCC 6 cut(s) 237, 359, 390, 468, 584, 711
BshNI GGYRCC 2 cut(s) 10, 287
BsiEI CGRYCG 2 cut(s) 469, 712
BsiHKAI GWGCWC 1 cut(s) 882
BsiHKCI CYCGRG 2 cut(s) 585, 881
BsiSI CCGG 9 cut(s) 85, 98, 230, 531, 572, 586, 630, 712, 746
BsiWI CGTACG 1 cut(s) 321
BslFI GGGAC 4 cut(s) 389, 590, 898, 1207
BslI CCNNNNNNNGG 7 cut(s) 97, 166, 229, 711, 779, 894, 1062
BsmAI GTCTC 1 cut(s) 1097
BsmFI GGGAC 4 cut(s) 389, 590, 898, 1207
BsnI GGCC 6 cut(s) 237, 359, 390, 468, 584, 711
BsoBI CYCGRG 2 cut(s) 585, 881
Bsp119I TTCGAA 1 cut(s) 863
Bsp1286I GDGCHC 1 cut(s) 882
Bsp13I TCCGGA 1 cut(s) 84
Bsp143I GATC 4 cut(s) 555, 769, 805, 1204
BspANI GGCC 6 cut(s) 237, 359, 390, 468, 584, 711
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
BspLI GGNNCC 4 cut(s) 12, 89, 289, 886
BspPI GGATC 2 cut(s) 550, 764
BspT104I TTCGAA 1 cut(s) 863
BspT107I GGYRCC 2 cut(s) 10, 287
BsrBI CCGCTC 1 cut(s) 195
BsrDI GCAATG 2 cut(s) 1110, 1195
BsrFI RCCGGY 2 cut(s) 629, 711
BsrI ACTGG 4 cut(s) 21, 295, 541, 696
BssAI RCCGGY 2 cut(s) 629, 711
BssECI CCNNGG 7 cut(s) 307, 354, 585, 586, 746, 908, 1046
BssMI GATC 4 cut(s) 555, 769, 805, 1204
BssNI GRCGYC 4 cut(s) 11, 288, 315, 462
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 1131
Bst4CI ACNGT 4 cut(s) 484, 612, 680, 964
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 4 cut(s) 11, 288, 315, 462
BstAPI GCANNNNNTGC 1 cut(s) 998
BstBAI YACGTR 1 cut(s) 321
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 204, 331, 631
BstDEI CTNAG 2 cut(s) 601, 889
BstENI CCTNNNNNAGG 1 cut(s) 892
BstF5I GGATG 6 cut(s) 267, 378, 423, 822, 1027, 1249
BstFNI CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
BstH2I RGCGCY 4 cut(s) 14, 32, 71, 291
BstHHI GCGC 8 cut(s) 13, 31, 70, 189, 213, 290, 329, 669
BstKTI GATC 4 cut(s) 558, 772, 808, 1207
BstMAI GTCTC 1 cut(s) 1097
BstMBI GATC 4 cut(s) 555, 769, 805, 1204
BstMCI CGRYCG 2 cut(s) 469, 712
BstNI CCWGG 1 cut(s) 1131
BstSCI CCNGG 6 cut(s) 96, 228, 584, 585, 745, 1129
BstUI CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
BstV1I GCAGC 4 cut(s) 64, 320, 383, 1225
BstV2I GAAGAC 2 cut(s) 433, 688
BstX2I RGATCY 1 cut(s) 1204
BstYI RGATCY 1 cut(s) 1204
BstZI CGGCCG 2 cut(s) 466, 709
Bsu36I CCTNAGG 1 cut(s) 889
BsuI GTATCC 1 cut(s) 1023
BsuRI GGCC 6 cut(s) 237, 359, 390, 468, 584, 711
BtrI CACGTC 2 cut(s) 415, 1286
BtsCI GGATG 6 cut(s) 267, 378, 423, 822, 1027, 1249
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 3 cut(s) 204, 331, 631
CfoI GCGC 8 cut(s) 13, 31, 70, 189, 213, 290, 329, 669
Cfr10I RCCGGY 2 cut(s) 629, 711
Cfr13I GGNCC 5 cut(s) 169, 235, 358, 583, 885
Cfr9I CCCGGG 1 cut(s) 585
CseI GACGC 6 cut(s) 169, 178, 202, 323, 325, 692
Csp6I GTAC 5 cut(s) 247, 322, 648, 689, 766
CviAII CATG 1 cut(s) 244
CviQI GTAC 5 cut(s) 247, 322, 648, 689, 766
DdeI CTNAG 2 cut(s) 601, 889
DinI GGCGCC 2 cut(s) 12, 289
DpnI GATC 4 cut(s) 557, 771, 807, 1206
DpnII GATC 4 cut(s) 555, 769, 805, 1204
EaeI YGGCCR 3 cut(s) 388, 466, 709
EagI CGGCCG 2 cut(s) 466, 709
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
EciI GGCGGA 1 cut(s) 616
EclXI CGGCCG 2 cut(s) 466, 709
Eco130I CCWWGG 1 cut(s) 354
Eco47I GGWCC 2 cut(s) 169, 885
Eco52I CGGCCG 2 cut(s) 466, 709
Eco57I CTGAAG 2 cut(s) 902, 1221
Eco81I CCTNAGG 1 cut(s) 889
Eco88I CYCGRG 2 cut(s) 585, 881
EcoNI CCTNNNNNAGG 1 cut(s) 892
EcoO109I RGGNCCY 3 cut(s) 235, 358, 885
EcoRII CCWGG 1 cut(s) 1129
EcoT14I CCWWGG 1 cut(s) 354
EgeI GGCGCC 2 cut(s) 12, 289
EheI GGCGCC 2 cut(s) 12, 289
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 247
FalI AAGNNNNNCTT 2 cut(s) 985, 1017
FaqI GGGAC 4 cut(s) 389, 590, 898, 1207
FatI CATG 1 cut(s) 243
FbaI TGATCA 1 cut(s) 805
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 9 cut(s) 53, 132, 286, 334, 351, 397, 469, 564, 1239
FokI GGATG 6 cut(s) 254, 365, 410, 829, 1034, 1256
Fsp4HI GCNGC 9 cut(s) 53, 132, 286, 334, 351, 397, 469, 564, 1239
FspBI CTAG 2 cut(s) 521, 1314
GlaI GCGC 8 cut(s) 12, 30, 69, 188, 212, 289, 328, 668
GluI GCNGC 9 cut(s) 53, 132, 286, 334, 351, 397, 469, 564, 1239
GsuI CTGGAG 3 cut(s) 206, 599, 815
HaeII RGCGCY 4 cut(s) 14, 32, 71, 291
HaeIII GGCC 6 cut(s) 237, 359, 390, 468, 584, 711
HapII CCGG 9 cut(s) 85, 98, 230, 531, 572, 586, 630, 712, 746
HgaI GACGC 6 cut(s) 169, 178, 202, 323, 325, 692
HhaI GCGC 8 cut(s) 13, 31, 70, 189, 213, 290, 329, 669
Hin1I GRCGYC 4 cut(s) 11, 288, 315, 462
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 8 cut(s) 11, 29, 68, 187, 211, 288, 327, 667
HinP1I GCGC 8 cut(s) 11, 29, 68, 187, 211, 288, 327, 667
HincII GTYRAC 2 cut(s) 340, 1273
HindII GTYRAC 2 cut(s) 340, 1273
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 8 cut(s) 41, 101, 824, 904, 913, 1013, 1160, 1301
HpaII CCGG 9 cut(s) 85, 98, 230, 531, 572, 586, 630, 712, 746
HphI GGTGA 3 cut(s) 409, 683, 692
Hpy166II GTNNAC 4 cut(s) 172, 340, 1077, 1273
Hpy188I TCNGA 6 cut(s) 46, 265, 560, 909, 921, 979
Hpy188III TCNNGA 6 cut(s) 85, 478, 722, 794, 883, 1102
Hpy8I GTNNAC 4 cut(s) 172, 340, 1077, 1273
Hpy99I CGWCG 9 cut(s) 218, 317, 341, 344, 449, 464, 467, 704, 710
HpyAV CCTTC 3 cut(s) 129, 289, 940
HpyCH4III ACNGT 4 cut(s) 484, 612, 680, 964
HpyCH4IV ACGT 5 cut(s) 320, 414, 438, 462, 1285
HpyCH4V TGCA 3 cut(s) 839, 992, 1307
HpyF3I CTNAG 2 cut(s) 601, 889
HpySE526I ACGT 5 cut(s) 320, 414, 438, 462, 1285
Hsp92I GRCGYC 4 cut(s) 11, 288, 315, 462
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 8 cut(s) 11, 29, 68, 187, 211, 288, 327, 667
KasI GGCGCC 2 cut(s) 10, 287
Kpn2I TCCGGA 1 cut(s) 84
KroI GCCGGC 1 cut(s) 629
KroNI GCCGGC 1 cut(s) 631
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 4 cut(s) 555, 769, 805, 1204
LmnI GCTCC 4 cut(s) 87, 200, 231, 574
Lsp1109I GCAGC 4 cut(s) 64, 320, 383, 1225
LweI GCATC 1 cut(s) 1012
MaeI CTAG 2 cut(s) 521, 1314
MaeII ACGT 5 cut(s) 320, 414, 438, 462, 1285
MaeIII GTNAC 2 cut(s) 415, 680
MalI GATC 4 cut(s) 557, 771, 807, 1206
MbiI CCGCTC 1 cut(s) 195
MboI GATC 4 cut(s) 555, 769, 805, 1204
MboII GAAGA 7 cut(s) 433, 688, 844, 908, 943, 946, 1214
MflI RGATCY 1 cut(s) 1204
MhlI GDGCHC 1 cut(s) 882
MluCI AATT 3 cut(s) 865, 955, 996
Mly113I GGCGCC 2 cut(s) 11, 288
MlyI GAGTC 4 cut(s) 35, 95, 833, 1007
MmeI TCCRAC 3 cut(s) 177, 288, 957
MreI CGCCGGCG 1 cut(s) 629
MroI TCCGGA 1 cut(s) 84
MroNI GCCGGC 1 cut(s) 629
MroXI GAANNNNTTC 2 cut(s) 77, 1097
MseI TTAA 3 cut(s) 473, 753, 954
MspA1I CMGCKG 1 cut(s) 396
MspI CCGG 9 cut(s) 85, 98, 230, 531, 572, 586, 630, 712, 746
MspR9I CCNGG 6 cut(s) 98, 230, 586, 587, 747, 1131
MvaI CCWGG 1 cut(s) 1131
MvnI CGCG 6 cut(s) 189, 211, 213, 329, 667, 669
NaeI GCCGGC 1 cut(s) 631
NarI GGCGCC 2 cut(s) 11, 288
NciI CCSGG 5 cut(s) 98, 230, 586, 587, 747
NdeII GATC 4 cut(s) 555, 769, 805, 1204
NgoMIV GCCGGC 1 cut(s) 629
NlaIII CATG 1 cut(s) 247
NlaIV GGNNCC 4 cut(s) 12, 89, 289, 886
NmeAIII GCCGAG 3 cut(s) 15, 288, 1027
NmuCI GTSAC 2 cut(s) 415, 680
NspV TTCGAA 1 cut(s) 863
PcsI WCGNNNNNNNCGW 6 cut(s) 42, 210, 261, 309, 326, 444
PdiI GCCGGC 1 cut(s) 631
PdmI GAANNNNTTC 2 cut(s) 77, 1097
PfeI GAWTC 4 cut(s) 904, 913, 1160, 1301
Pfl23II CGTACG 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 1062
PfoI TCCNGGA 2 cut(s) 96, 228
PkrI GCNGC 9 cut(s) 54, 133, 287, 335, 352, 398, 470, 565, 1240
PleI GAGTC 4 cut(s) 35, 95, 832, 1007
PluTI GGCGCC 2 cut(s) 14, 291
PpsI GAGTC 4 cut(s) 35, 95, 832, 1007
Ppu21I YACGTR 1 cut(s) 321
PpuMI RGGWCCY 1 cut(s) 885
Psp5II RGGWCCY 1 cut(s) 885
Psp6I CCWGG 1 cut(s) 1129
PspGI CCWGG 1 cut(s) 1129
PspLI CGTACG 1 cut(s) 321
PspN4I GGNNCC 4 cut(s) 12, 89, 289, 886
PspPI GGNCC 5 cut(s) 169, 235, 358, 583, 885
PspPPI RGGWCCY 1 cut(s) 885
PsuI RGATCY 1 cut(s) 1204
PvuII CAGCTG 1 cut(s) 396
RsaI GTAC 5 cut(s) 248, 323, 649, 690, 767
RsaNI GTAC 5 cut(s) 247, 322, 648, 689, 766
SalI GTCGAC 1 cut(s) 338
SaqAI TTAA 3 cut(s) 473, 753, 954
SatI GCNGC 9 cut(s) 53, 132, 286, 334, 351, 397, 469, 564, 1239
Sau3AI GATC 4 cut(s) 555, 769, 805, 1204
Sau96I GGNCC 5 cut(s) 169, 235, 358, 583, 885
SchI GAGTC 4 cut(s) 35, 95, 833, 1007
ScrFI CCNGG 6 cut(s) 98, 230, 586, 587, 747, 1131
SduI GDGCHC 1 cut(s) 882
SfaNI GCATC 1 cut(s) 1012
SfoI GGCGCC 2 cut(s) 12, 289
SfuI TTCGAA 1 cut(s) 863
SgrAI CRCCGGYG 1 cut(s) 629
SgrDI CGTCGACG 1 cut(s) 338
SinI GGWCC 2 cut(s) 169, 885
SmaI CCCGGG 1 cut(s) 587
Sse9I AATT 3 cut(s) 865, 955, 996
SspDI GGCGCC 2 cut(s) 10, 287
SspMI CTAG 2 cut(s) 521, 1314
StyD4I CCNGG 6 cut(s) 96, 228, 584, 585, 745, 1129
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 4 cut(s) 484, 612, 680, 964
TaiI ACGT 5 cut(s) 323, 417, 441, 465, 1288
TaqI TCGA 8 cut(s) 165, 339, 447, 459, 614, 827, 863, 1101
TasI AATT 3 cut(s) 865, 955, 996
TatI WGTACW 2 cut(s) 246, 688
TauI GCSGC 5 cut(s) 134, 288, 353, 471, 566
TfiI GAWTC 4 cut(s) 904, 913, 1160, 1301
Tru1I TTAA 3 cut(s) 473, 753, 954
Tru9I TTAA 3 cut(s) 473, 753, 954
TscAI CASTG 1 cut(s) 302
TseFI GTSAC 2 cut(s) 415, 680
TseI GCWGC 4 cut(s) 52, 333, 396, 1238
Tsp45I GTSAC 2 cut(s) 415, 680
TspDTI ATGAA 1 cut(s) 707
TspGWI ACGGA 1 cut(s) 762
TspMI CCCGGG 1 cut(s) 585
TspRI CASTG 1 cut(s) 302
Van91I CCANNNNNTGG 1 cut(s) 1062
VpaK11BI GGWCC 2 cut(s) 169, 885
XagI CCTNNNNNAGG 1 cut(s) 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmaI CCCGGG 1 cut(s) 585
XmiI GTMKAC 1 cut(s) 339
XmnI GAANNNNTTC 2 cut(s) 77, 1097
XspI CTAG 2 cut(s) 521, 1314
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.