Rw7G031440

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0010480
Physical Location & Seq
Reverse (-)
45051003 .. 45055626
4624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G031440.1

Sequence Viewer

Length: 1341 bp
ATGGAGGAGGCGCAAAAGCCAAGTCTCGCTCTTCTCGGCGACTCAGAACAGCGAAAGCCACAAGCGCCGAAGCGGTTCGTCCGGAGCCAAATCCCGGACTCAATACTCCACGACCCGGCTCTCAACGCCGCCGTAAGCCTCCTCCCCTCCAACTACCACTTCGAAGTCCACAAATGCGTCTGGCGCGTCCGCTCCACGAACGCCTCGCGCGTCGCTCTCCAGCTGCCGGAGGGCCTCCTGATGTACTCCCTCGTCCTCTCCGATATTCTCTCCACCTTCGGAGGCGCCTCCCAGTGCTTCGTTCTCGGCGACGCCACTTACGGCGCGTGCTGCGTCGACGACCTCGCCGCCAAGGCCCTCAACGCCGACCTCCTCATTCACTTCGGCCACAGCTGCCTCGTCCCCCTCCACGTCACCTCCATCCCCTGCCTCTACGTCTTCGTCGAAATCTCCATCGACGTCGGCCGGTTAATCCAGACCGTCCATCTCAACCTCCAAAACCCTCTCCCTAAAACCCTAGTCCTCGCCGGAACAATCCAGTTCGCTTCGGCGATTCGAGCGGCCAAGCCCGAGCTTGAAGCCCGGGGTTTCAAGGTCTTGGTCCCACAGTCTAAGCCCTTGTCAGCCGGCGAGGTTTTGGGCTGTACAGCCCCGAAAATCTCACGAAACCACGGCGGTGAGGACGGCGGTGACACTGTACTGGTATTCGTGGCCGACGGCCGGTTTCATCTGGAAGCTTTTATGATAGCAAATCCAGGGGTTAAGGCGTTTCGGTACGATCCTTATATGGGGAAGCTGTTTCTGGAGGAATATGATCACAAAGGGATGAGGGAGTCGAGAAAGAGTGCCGTGTTGAGAGCCAAAGAGGCCTCCAATTGGGGCATTGTGCTTGGGACTTTAGGAAGGCAAGGGAATCCGAGGATTCTACAGAGATTGGAGAAGAAGATGAGGGAGAAGGGGTTTGATTATACTGTGGTGTTAATGTCGGAGATTAGTCCGGCGAGAATTGCTTTGTTTGAGGACTCTGTGGATGCTTGGATACAGATTGCTTGTCCTCGCTTGTCAATTGATTGGGGGGATGCATTTACGAAGCCCCTTTTGAATCCTTTCGAGACTGACATTGCTCTCGGCTTCATTCCTGGTTGGTGGGAGAGGGACAAGAAAAGTGATTCGGGGTGCTGTGCTTGTGGTAATGGCAATGGGACTGCGGAAGACCTTGTAGGGGACTATCCCATGGATTACTATGCTGCAGATGGAGGGGAGTGGAACTCCTCTTATGTCAACAAGCCGACACGCCCTGTTCGCAGGGATTCCGCATTGGCAACTGCCAACATTGAGTAG

Protein Analysis

446

Amino Acids

48.91

Weight (kDa)

6.23

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 75 - 383 1.7e-105 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 462
AccB1I GGYRCC 1 cut(s) 284
AccBSI CCGCTC 2 cut(s) 192, 560
AccI GTMKAC 1 cut(s) 336
AccII CGCG 4 cut(s) 186, 208, 210, 326
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 9 cut(s) 73, 129, 190, 348, 560, 675, 687, 1208, 1314
AclWI GGATC 1 cut(s) 773
AcoI YGGCCR 5 cut(s) 385, 463, 561, 711, 718
AcyI GRCGYC 3 cut(s) 285, 312, 459
AfaI GTAC 4 cut(s) 245, 646, 699, 776
AfiI CCNNNNNNNGG 7 cut(s) 94, 115, 226, 720, 788, 876, 1222
AgsI TTSAA 3 cut(s) 578, 592, 1102
AjiI CACGTC 1 cut(s) 412
AjnI CCWGG 2 cut(s) 754, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 143, 175
AleI CACNNNNGTG 1 cut(s) 675
AluBI AGCT 5 cut(s) 223, 393, 574, 737, 796
AluI AGCT 5 cut(s) 223, 393, 574, 737, 796
Alw26I GTCTC 2 cut(s) 29, 1106
AlwI GGATC 1 cut(s) 773
Ama87I CYCGRG 2 cut(s) 569, 582
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 8 cut(s) 232, 354, 385, 463, 561, 711, 718, 867
ApeKI GCWGC 4 cut(s) 223, 330, 393, 1247
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 2 cut(s) 74, 1106
AspLEI GCGC 6 cut(s) 13, 67, 186, 210, 287, 326
AspS9I GGNCC 3 cut(s) 232, 355, 601
AsuC2I CCSGG 4 cut(s) 95, 116, 583, 584
AsuHPI GGTGA 3 cut(s) 406, 689, 701
AsuII TTCGAA 1 cut(s) 162
AvaI CYCGRG 2 cut(s) 569, 582
AvaII GGWCC 1 cut(s) 601
BanI GGYRCC 1 cut(s) 284
BbsI GAAGAC 2 cut(s) 430, 1218
BbvI GCAGC 4 cut(s) 210, 317, 380, 1234
BccI CCATC 4 cut(s) 428, 461, 492, 1247
BceAI ACGGC 6 cut(s) 116, 337, 688, 700, 733, 833
BciT130I CCWGG 2 cut(s) 756, 1140
BciVI GTATCC 1 cut(s) 1032
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 4 cut(s) 95, 116, 583, 584
BcoDI GTCTC 2 cut(s) 29, 1106
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 2 cut(s) 926, 1248
BfoI RGCGCY 2 cut(s) 68, 288
BfuI GTATCC 1 cut(s) 1032
BglI GCCNNNNNGGC 2 cut(s) 353, 866
BisI GCNGC 7 cut(s) 129, 224, 331, 348, 394, 561, 1248
BlsI GCNGC 7 cut(s) 130, 225, 332, 349, 395, 562, 1249
Bme1390I CCNGG 6 cut(s) 95, 116, 583, 584, 756, 1140
Bme18I GGWCC 1 cut(s) 601
BmeT110I CYCGRG 2 cut(s) 569, 582
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 232, 355, 601
BmiI GGNNCC 3 cut(s) 86, 286, 603
BmrFI CCNGG 6 cut(s) 95, 116, 583, 584, 756, 1140
BmrI ACTGGG 1 cut(s) 286
BmsI GCATC 2 cut(s) 1021, 1069
BmuI ACTGGG 1 cut(s) 286
BpiI GAAGAC 2 cut(s) 430, 1218
BplI GAGNNNNNCTC 2 cut(s) 1253, 1285
BpmI CTGGAG 2 cut(s) 203, 824
Bpu14I TTCGAA 1 cut(s) 162
BpuMI CCSGG 4 cut(s) 95, 116, 583, 584
BsaHI GRCGYC 3 cut(s) 285, 312, 459
BsaJI CCNNGG 7 cut(s) 351, 582, 583, 670, 755, 917, 1233
BsaWI WCCGGW 1 cut(s) 81
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 944, 974
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 115, 226, 720, 788, 876, 1222
Bse118I RCCGGY 3 cut(s) 465, 626, 720
Bse1I ACTGG 3 cut(s) 292, 538, 705
Bse3DI GCAATG 2 cut(s) 1119, 1204
BseAI TCCGGA 1 cut(s) 81
BseBI CCWGG 2 cut(s) 756, 1140
BseDI CCNNGG 7 cut(s) 351, 582, 583, 670, 755, 917, 1233
BseGI GGATG 4 cut(s) 420, 831, 1036, 1084
BseLI CCNNNNNNNGG 7 cut(s) 94, 115, 226, 720, 788, 876, 1222
BseMI GCAATG 2 cut(s) 1119, 1204
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 3 cut(s) 292, 538, 705
BseRI GAGGAG 4 cut(s) 20, 131, 362, 1261
BseX3I CGGCCG 2 cut(s) 463, 718
BseXI GCAGC 4 cut(s) 210, 317, 380, 1234
Bsh1236I CGCG 4 cut(s) 186, 208, 210, 326
Bsh1285I CGRYCG 2 cut(s) 466, 721
BshFI GGCC 8 cut(s) 234, 356, 387, 465, 563, 713, 720, 869
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 2 cut(s) 466, 721
BsiHKCI CYCGRG 2 cut(s) 569, 582
BslFI GGGAC 6 cut(s) 386, 587, 907, 1169, 1216, 1238
BslI CCNNNNNNNGG 7 cut(s) 94, 115, 226, 720, 788, 876, 1222
BsmAI GTCTC 2 cut(s) 29, 1106
BsmFI GGGAC 6 cut(s) 386, 587, 907, 1169, 1216, 1238
BsnI GGCC 8 cut(s) 234, 356, 387, 465, 563, 713, 720, 869
BsoBI CYCGRG 2 cut(s) 569, 582
Bsp119I TTCGAA 1 cut(s) 162
Bsp13I TCCGGA 1 cut(s) 81
Bsp1407I TGTACA 1 cut(s) 644
Bsp143I GATC 2 cut(s) 778, 814
Bsp19I CCATGG 1 cut(s) 1233
BspACI CCGC 9 cut(s) 73, 129, 190, 348, 560, 675, 687, 1208, 1314
BspANI GGCC 8 cut(s) 234, 356, 387, 465, 563, 713, 720, 869
BspCNI CTCAG 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 81
BspFNI CGCG 4 cut(s) 186, 208, 210, 326
BspLI GGNNCC 3 cut(s) 86, 286, 603
BspMAI CTGCAG 1 cut(s) 1252
BspPI GGATC 1 cut(s) 773
BspQI GCTCTTC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 284
BsrBI CCGCTC 2 cut(s) 192, 560
BsrDI GCAATG 2 cut(s) 1119, 1204
BsrFI RCCGGY 3 cut(s) 465, 626, 720
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 3 cut(s) 292, 538, 705
BssAI RCCGGY 3 cut(s) 465, 626, 720
BssECI CCNNGG 7 cut(s) 351, 582, 583, 670, 755, 917, 1233
BssMI GATC 2 cut(s) 778, 814
BssNI GRCGYC 3 cut(s) 285, 312, 459
BssT1I CCWWGG 2 cut(s) 351, 1233
Bst2UI CCWGG 2 cut(s) 756, 1140
Bst4CI ACNGT 4 cut(s) 481, 609, 697, 973
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 3 cut(s) 285, 312, 459
BstAUI TGTACA 1 cut(s) 644
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 328, 628
BstDEI CTNAG 2 cut(s) 43, 612
BstDSI CCRYGG 2 cut(s) 670, 1233
BstF5I GGATG 4 cut(s) 420, 831, 1036, 1084
BstFNI CGCG 4 cut(s) 186, 208, 210, 326
BstH2I RGCGCY 2 cut(s) 68, 288
BstHHI GCGC 6 cut(s) 13, 67, 186, 210, 287, 326
BstKTI GATC 2 cut(s) 781, 817
BstMAI GTCTC 2 cut(s) 29, 1106
BstMBI GATC 2 cut(s) 778, 814
BstMCI CGRYCG 2 cut(s) 466, 721
BstNI CCWGG 2 cut(s) 756, 1140
BstSCI CCNGG 6 cut(s) 93, 114, 581, 582, 754, 1138
BstSFI CTRYAG 2 cut(s) 926, 1248
BstUI CGCG 4 cut(s) 186, 208, 210, 326
BstV1I GCAGC 4 cut(s) 210, 317, 380, 1234
BstV2I GAAGAC 2 cut(s) 430, 1218
BstZI CGGCCG 2 cut(s) 463, 718
BsuI GTATCC 1 cut(s) 1032
BsuRI GGCC 8 cut(s) 234, 356, 387, 465, 563, 713, 720, 869
BtgI CCRYGG 2 cut(s) 670, 1233
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 4 cut(s) 420, 831, 1036, 1084
BtsIMutI CAGTG 2 cut(s) 299, 693
Cac8I GCNNGC 2 cut(s) 328, 628
CfoI GCGC 6 cut(s) 13, 67, 186, 210, 287, 326
Cfr10I RCCGGY 3 cut(s) 465, 626, 720
Cfr13I GGNCC 3 cut(s) 232, 355, 601
Cfr9I CCCGGG 1 cut(s) 582
CseI GACGC 5 cut(s) 166, 175, 199, 320, 322
Csp6I GTAC 4 cut(s) 244, 645, 698, 775
CviAII CATG 1 cut(s) 1234
CviQI GTAC 4 cut(s) 244, 645, 698, 775
DdeI CTNAG 2 cut(s) 43, 612
DinI GGCGCC 1 cut(s) 286
DpnI GATC 2 cut(s) 780, 816
DpnII GATC 2 cut(s) 778, 814
EaeI YGGCCR 5 cut(s) 385, 463, 561, 711, 718
EagI CGGCCG 2 cut(s) 463, 718
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EclXI CGGCCG 2 cut(s) 463, 718
Eco130I CCWWGG 2 cut(s) 351, 1233
Eco147I AGGCCT 1 cut(s) 869
Eco47I GGWCC 1 cut(s) 601
Eco52I CGGCCG 2 cut(s) 463, 718
Eco88I CYCGRG 2 cut(s) 569, 582
EcoO109I RGGNCCY 2 cut(s) 232, 355
EcoRII CCWGG 2 cut(s) 754, 1138
EcoT14I CCWWGG 2 cut(s) 351, 1233
EcoT22I ATGCAT 1 cut(s) 1084
EgeI GGCGCC 1 cut(s) 286
EheI GGCGCC 1 cut(s) 286
ErhI CCWWGG 2 cut(s) 351, 1233
FaeI CATG 1 cut(s) 1237
FaiI YATR 8 cut(s) 743, 786, 788, 813, 969, 1235, 1245, 1278
FaqI GGGAC 6 cut(s) 386, 587, 907, 1169, 1216, 1238
FatI CATG 1 cut(s) 1233
FbaI TGATCA 1 cut(s) 814
FblI GTMKAC 1 cut(s) 336
Fnu4HI GCNGC 7 cut(s) 129, 224, 331, 348, 394, 561, 1248
FokI GGATG 4 cut(s) 407, 838, 1043, 1091
Fsp4HI GCNGC 7 cut(s) 129, 224, 331, 348, 394, 561, 1248
FspBI CTAG 1 cut(s) 518
GlaI GCGC 6 cut(s) 12, 66, 185, 209, 286, 325
GluI GCNGC 7 cut(s) 129, 224, 331, 348, 394, 561, 1248
GsuI CTGGAG 2 cut(s) 203, 824
HaeII RGCGCY 2 cut(s) 68, 288
HaeIII GGCC 8 cut(s) 234, 356, 387, 465, 563, 713, 720, 869
HgaI GACGC 5 cut(s) 166, 175, 199, 320, 322
HhaI GCGC 6 cut(s) 13, 67, 186, 210, 287, 326
Hin1I GRCGYC 3 cut(s) 285, 312, 459
Hin1II CATG 1 cut(s) 1237
Hin6I GCGC 6 cut(s) 11, 65, 184, 208, 285, 324
HinP1I GCGC 6 cut(s) 11, 65, 184, 208, 285, 324
HincII GTYRAC 2 cut(s) 337, 1282
HindII GTYRAC 2 cut(s) 337, 1282
HindIII AAGCTT 1 cut(s) 735
HphI GGTGA 3 cut(s) 406, 689, 701
Hpy166II GTNNAC 3 cut(s) 169, 337, 1282
Hpy188I TCNGA 5 cut(s) 46, 262, 281, 918, 988
Hpy188III TCNNGA 8 cut(s) 82, 238, 475, 663, 731, 803, 837, 1111
Hpy8I GTNNAC 3 cut(s) 169, 337, 1282
Hpy99I CGWCG 8 cut(s) 215, 314, 338, 341, 446, 461, 464, 719
HpyAV CCTTC 3 cut(s) 286, 897, 949
HpyCH4III ACNGT 4 cut(s) 481, 609, 697, 973
HpyCH4IV ACGT 3 cut(s) 411, 435, 459
HpyCH4V TGCA 2 cut(s) 1082, 1250
HpyF3I CTNAG 2 cut(s) 43, 612
HpySE526I ACGT 3 cut(s) 411, 435, 459
Hsp92I GRCGYC 3 cut(s) 285, 312, 459
Hsp92II CATG 1 cut(s) 1237
HspAI GCGC 6 cut(s) 11, 65, 184, 208, 285, 324
KasI GGCGCC 1 cut(s) 284
Kpn2I TCCGGA 1 cut(s) 81
KroI GCCGGC 1 cut(s) 626
KroNI GCCGGC 1 cut(s) 628
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 2 cut(s) 778, 814
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 84, 197
Lsp1109I GCAGC 4 cut(s) 210, 317, 380, 1234
LweI GCATC 2 cut(s) 1021, 1069
MaeI CTAG 1 cut(s) 518
MaeII ACGT 3 cut(s) 411, 435, 459
MaeIII GTNAC 2 cut(s) 412, 689
MalI GATC 2 cut(s) 780, 816
MbiI CCGCTC 2 cut(s) 192, 560
MboI GATC 2 cut(s) 778, 814
MboII GAAGA 5 cut(s) 23, 430, 952, 955, 1223
MfeI CAATTG 2 cut(s) 874, 1065
MluCI AATT 3 cut(s) 874, 1005, 1065
Mly113I GGCGCC 1 cut(s) 285
MlyI GAGTC 4 cut(s) 35, 92, 842, 1016
MmeI TCCRAC 2 cut(s) 174, 966
Mph1103I ATGCAT 1 cut(s) 1084
MroI TCCGGA 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 626
MroXI GAANNNNTTC 2 cut(s) 74, 1106
MseI TTAA 3 cut(s) 470, 762, 980
MslI CAYNNNNRTG 1 cut(s) 675
MspA1I CMGCKG 2 cut(s) 223, 393
MspR9I CCNGG 6 cut(s) 95, 116, 583, 584, 756, 1140
MunI CAATTG 2 cut(s) 874, 1065
MvaI CCWGG 2 cut(s) 756, 1140
MvnI CGCG 4 cut(s) 186, 208, 210, 326
NaeI GCCGGC 1 cut(s) 628
NarI GGCGCC 1 cut(s) 285
NciI CCSGG 4 cut(s) 95, 116, 583, 584
NcoI CCATGG 1 cut(s) 1233
NdeII GATC 2 cut(s) 778, 814
NgoMIV GCCGGC 1 cut(s) 626
NlaIII CATG 1 cut(s) 1237
NlaIV GGNNCC 3 cut(s) 86, 286, 603
NmeAIII GCCGAG 3 cut(s) 15, 285, 1107
NmuCI GTSAC 2 cut(s) 412, 689
NsiI ATGCAT 1 cut(s) 1084
NspV TTCGAA 1 cut(s) 162
OliI CACNNNNGTG 1 cut(s) 675
PceI AGGCCT 1 cut(s) 869
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 4 cut(s) 207, 258, 306, 441
PdiI GCCGGC 1 cut(s) 628
PdmI GAANNNNTTC 2 cut(s) 74, 1106
PfeI GAWTC 6 cut(s) 553, 913, 922, 1102, 1169, 1310
PfoI TCCNGGA 1 cut(s) 93
PkrI GCNGC 7 cut(s) 130, 225, 332, 349, 395, 562, 1249
PleI GAGTC 4 cut(s) 35, 92, 841, 1016
PluTI GGCGCC 1 cut(s) 288
PpsI GAGTC 4 cut(s) 35, 92, 841, 1016
Psp6I CCWGG 2 cut(s) 754, 1138
PspGI CCWGG 2 cut(s) 754, 1138
PspN4I GGNNCC 3 cut(s) 86, 286, 603
PspPI GGNCC 3 cut(s) 232, 355, 601
PstI CTGCAG 1 cut(s) 1252
PvuII CAGCTG 2 cut(s) 223, 393
RsaI GTAC 4 cut(s) 245, 646, 699, 776
RsaNI GTAC 4 cut(s) 244, 645, 698, 775
RseI CAYNNNNRTG 1 cut(s) 675
SalI GTCGAC 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 3 cut(s) 470, 762, 980
SatI GCNGC 7 cut(s) 129, 224, 331, 348, 394, 561, 1248
Sau3AI GATC 2 cut(s) 778, 814
Sau96I GGNCC 3 cut(s) 232, 355, 601
SchI GAGTC 4 cut(s) 35, 92, 842, 1016
ScrFI CCNGG 6 cut(s) 95, 116, 583, 584, 756, 1140
SfaNI GCATC 2 cut(s) 1021, 1069
SfcI CTRYAG 2 cut(s) 926, 1248
SfoI GGCGCC 1 cut(s) 286
SfuI TTCGAA 1 cut(s) 162
SgrDI CGTCGACG 1 cut(s) 335
SinI GGWCC 1 cut(s) 601
SmaI CCCGGG 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 675
Sse9I AATT 3 cut(s) 874, 1005, 1065
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 9 cut(s) 73, 129, 190, 348, 560, 675, 687, 1208, 1314
SspDI GGCGCC 1 cut(s) 284
SspMI CTAG 1 cut(s) 518
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 6 cut(s) 93, 114, 581, 582, 754, 1138
StyI CCWWGG 2 cut(s) 351, 1233
TaaI ACNGT 4 cut(s) 481, 609, 697, 973
TaiI ACGT 3 cut(s) 414, 438, 462
TaqI TCGA 7 cut(s) 162, 336, 444, 456, 556, 836, 1110
TasI AATT 3 cut(s) 874, 1005, 1065
TatI WGTACW 3 cut(s) 243, 644, 697
TauI GCSGC 3 cut(s) 131, 350, 563
TfiI GAWTC 6 cut(s) 553, 913, 922, 1102, 1169, 1310
Tru1I TTAA 3 cut(s) 470, 762, 980
Tru9I TTAA 3 cut(s) 470, 762, 980
TscAI CASTG 2 cut(s) 299, 700
TseFI GTSAC 2 cut(s) 412, 689
TseI GCWGC 4 cut(s) 223, 330, 393, 1247
Tsp45I GTSAC 2 cut(s) 412, 689
TspDTI ATGAA 2 cut(s) 716, 1123
TspMI CCCGGG 1 cut(s) 582
TspRI CASTG 2 cut(s) 299, 700
VpaK11BI GGWCC 1 cut(s) 601
XmaI CCCGGG 1 cut(s) 582
XmiI GTMKAC 1 cut(s) 336
XmnI GAANNNNTTC 2 cut(s) 74, 1106
XspI CTAG 1 cut(s) 518
ZraI GACGTC 1 cut(s) 460
Zsp2I ATGCAT 1 cut(s) 1084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.