RchiOBHm_Chr7g0225101

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0010480
Physical Location & Seq
Reverse (-)
47526136 .. 47530138
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20159

Sequence Viewer

Length: 786 bp
ATGGAGGAGGCGCAAAAACCTAGTCTCGCGCTTCTCGGCGACTCAGAACAGCGAAAGCCACAAGCACCGAAGCGGTTCGTCCGGAGCCAAATCCCCGACTCAATACTCCACGACCCGGCACTCAACGCCGCCATAAGCCTCCTCCCCTCCAACTACCACTTCGAAGTCCACAAATGCGTCTGGCGCGTCCGCTCGCGAACGCCTCATGCTGCTTCTCTCGGCGACGCCACTTACGGCGCGTGCTGCGTCGACGACCTCGCCGCCAAGGCCCTCAACGCCGACCTCCTCATTCACTTCGGCCACAGCTGCCTCGTCCCCCTCCACGTCACCTCCATCCCCTGCCTCTACGTCTTCGTCGAAATCTCAATCGACGTCGGCCGGTTAATCCAGACCGTCCATCTCAACCTCCAAAACCCTCTCCCTAAAACCCTAGTCCTCGCCGGAACAATCCAGTTCGCTTCGGCGATTCGGGCGGCGAAGCCCGAGCTTGAAGCCCGGGGTTTCAAGGTCTTGGTCCCACAGTCTAAGCCCTTGTCCGCCGGCGAGGTTTTGGGGTGTACGGCCCCGAAAATCTCACGAAACCACGGCGGTGAGGACGGTGGAGACACTGTTCTGGTATTCGTGGCCGACGGCCGGTTTCATCTGGAAGCTTTTATGATAGCAAATCCAGGGGTTAAGGCGTTTCGGTATGATCCTTATATGGGGAAGCTGTTTTTGGAGGAATATGATCACAAAGGGATGAGGGAGTCGAGAAAGAGCGCCGTGTTGAGAGCCAAAGAAAAGTGA

Protein Analysis

261

Amino Acids

28.61

Weight (kDa)

8.7

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 72 - 260 1.4e-57 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 375
AccBSI CCGCTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 249
AccII CGCG 4 cut(s) 29, 186, 196, 239
AccIII TCCGGA 1 cut(s) 81
AciI CCGC 7 cut(s) 73, 129, 190, 261, 473, 537, 588
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 4 cut(s) 298, 376, 624, 631
AcyI GRCGYC 2 cut(s) 225, 372
AfaI GTAC 1 cut(s) 559
AfiI CCNNNNNNNGG 3 cut(s) 115, 633, 701
AgsI TTSAA 2 cut(s) 491, 505
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 667
AjuI GAANNNNNNNTTGG 4 cut(s) 143, 175, 698, 730
AleI CACNNNNGTG 1 cut(s) 588
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
AluBI AGCT 4 cut(s) 306, 487, 650, 709
AluI AGCT 4 cut(s) 306, 487, 650, 709
Alw26I GTCTC 2 cut(s) 29, 597
AlwI GGATC 1 cut(s) 686
Ama87I CYCGRG 2 cut(s) 482, 495
Aor13HI TCCGGA 1 cut(s) 81
AoxI GGCC 6 cut(s) 267, 298, 376, 561, 624, 631
ApeKI GCWGC 3 cut(s) 209, 243, 306
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 5 cut(s) 13, 31, 186, 239, 761
AspS9I GGNCC 3 cut(s) 268, 514, 562
AsuC2I CCSGG 3 cut(s) 116, 496, 497
AsuHPI GGTGA 2 cut(s) 319, 602
AsuII TTCGAA 1 cut(s) 162
AvaI CYCGRG 2 cut(s) 482, 495
AvaII GGWCC 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 343
BbvI GCAGC 3 cut(s) 196, 230, 293
BccI CCATC 2 cut(s) 341, 405
BceAI ACGGC 5 cut(s) 250, 576, 601, 646, 746
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 1 cut(s) 727
BcnI CCSGG 3 cut(s) 116, 496, 497
BcoDI GTCTC 2 cut(s) 29, 597
BfaI CTAG 2 cut(s) 21, 431
BfoI RGCGCY 1 cut(s) 762
BglI GCCNNNNNGGC 1 cut(s) 266
BisI GCNGC 6 cut(s) 129, 210, 244, 261, 307, 474
BlsI GCNGC 6 cut(s) 130, 211, 245, 262, 308, 475
Bme1390I CCNGG 4 cut(s) 116, 496, 497, 669
Bme18I GGWCC 1 cut(s) 514
BmeT110I CYCGRG 2 cut(s) 482, 495
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 268, 514, 562
BmiI GGNNCC 3 cut(s) 86, 516, 564
BmrFI CCNGG 4 cut(s) 116, 496, 497, 669
BpiI GAAGAC 1 cut(s) 343
Bpu14I TTCGAA 1 cut(s) 162
BpuMI CCSGG 3 cut(s) 116, 496, 497
BsaHI GRCGYC 2 cut(s) 225, 372
BsaJI CCNNGG 5 cut(s) 264, 495, 496, 583, 668
BsaWI WCCGGW 1 cut(s) 81
BsaXI ACNNNNNCTCC 4 cut(s) 314, 344, 594, 624
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 633, 701
Bse118I RCCGGY 3 cut(s) 378, 539, 633
Bse1I ACTGG 1 cut(s) 451
BseAI TCCGGA 1 cut(s) 81
BseBI CCWGG 1 cut(s) 669
BseDI CCNNGG 5 cut(s) 264, 495, 496, 583, 668
BseGI GGATG 2 cut(s) 333, 744
BseLI CCNNNNNNNGG 3 cut(s) 115, 633, 701
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 451
BseRI GAGGAG 3 cut(s) 20, 131, 275
BseX3I CGGCCG 2 cut(s) 376, 631
BseXI GCAGC 3 cut(s) 196, 230, 293
Bsh1236I CGCG 4 cut(s) 29, 186, 196, 239
Bsh1285I CGRYCG 2 cut(s) 379, 634
BshFI GGCC 6 cut(s) 269, 300, 378, 563, 626, 633
BsiEI CGRYCG 2 cut(s) 379, 634
BsiHKCI CYCGRG 2 cut(s) 482, 495
BsiSI CCGG 7 cut(s) 82, 116, 379, 441, 496, 540, 634
BslFI GGGAC 2 cut(s) 299, 500
BslI CCNNNNNNNGG 3 cut(s) 115, 633, 701
BsmAI GTCTC 2 cut(s) 29, 597
BsmFI GGGAC 2 cut(s) 299, 500
BsnI GGCC 6 cut(s) 269, 300, 378, 563, 626, 633
BsoBI CYCGRG 2 cut(s) 482, 495
Bsp119I TTCGAA 1 cut(s) 162
Bsp13I TCCGGA 1 cut(s) 81
Bsp143I GATC 2 cut(s) 691, 727
Bsp68I TCGCGA 1 cut(s) 196
BspACI CCGC 7 cut(s) 73, 129, 190, 261, 473, 537, 588
BspANI GGCC 6 cut(s) 269, 300, 378, 563, 626, 633
BspCNI CTCAG 1 cut(s) 56
BspEI TCCGGA 1 cut(s) 81
BspFNI CGCG 4 cut(s) 29, 186, 196, 239
BspLI GGNNCC 3 cut(s) 86, 516, 564
BspPI GGATC 1 cut(s) 686
BspT104I TTCGAA 1 cut(s) 162
BsrBI CCGCTC 1 cut(s) 192
BsrFI RCCGGY 3 cut(s) 378, 539, 633
BsrI ACTGG 1 cut(s) 451
BssAI RCCGGY 3 cut(s) 378, 539, 633
BssECI CCNNGG 5 cut(s) 264, 495, 496, 583, 668
BssMI GATC 2 cut(s) 691, 727
BssNI GRCGYC 2 cut(s) 225, 372
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 4 cut(s) 394, 522, 599, 610
BstACI GRCGYC 2 cut(s) 225, 372
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 3 cut(s) 194, 241, 541
BstDEI CTNAG 2 cut(s) 43, 525
BstDSI CCRYGG 1 cut(s) 583
BstF5I GGATG 2 cut(s) 333, 744
BstFNI CGCG 4 cut(s) 29, 186, 196, 239
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 5 cut(s) 13, 31, 186, 239, 761
BstKTI GATC 2 cut(s) 694, 730
BstMAI GTCTC 2 cut(s) 29, 597
BstMBI GATC 2 cut(s) 691, 727
BstMCI CGRYCG 2 cut(s) 379, 634
BstMWI GCNNNNNNNGC 7 cut(s) 125, 183, 243, 266, 275, 306, 470
BstNI CCWGG 1 cut(s) 669
BstSCI CCNGG 4 cut(s) 114, 494, 495, 667
BstUI CGCG 4 cut(s) 29, 186, 196, 239
BstV1I GCAGC 3 cut(s) 196, 230, 293
BstV2I GAAGAC 1 cut(s) 343
BstZI CGGCCG 2 cut(s) 376, 631
BsuRI GGCC 6 cut(s) 269, 300, 378, 563, 626, 633
BtgI CCRYGG 1 cut(s) 583
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 2 cut(s) 333, 744
BtsIMutI CAGTG 1 cut(s) 606
BtuMI TCGCGA 1 cut(s) 196
Cac8I GCNNGC 3 cut(s) 194, 241, 541
CfoI GCGC 5 cut(s) 13, 31, 186, 239, 761
Cfr10I RCCGGY 3 cut(s) 378, 539, 633
Cfr13I GGNCC 3 cut(s) 268, 514, 562
Cfr9I CCCGGG 1 cut(s) 495
CseI GACGC 4 cut(s) 166, 175, 233, 235
Csp6I GTAC 1 cut(s) 558
CviAII CATG 1 cut(s) 206
CviQI GTAC 1 cut(s) 558
DdeI CTNAG 2 cut(s) 43, 525
DpnI GATC 2 cut(s) 693, 729
DpnII GATC 2 cut(s) 691, 727
EaeI YGGCCR 4 cut(s) 298, 376, 624, 631
EagI CGGCCG 2 cut(s) 376, 631
EciI GGCGGA 1 cut(s) 526
EclXI CGGCCG 2 cut(s) 376, 631
Eco130I CCWWGG 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 514
Eco52I CGGCCG 2 cut(s) 376, 631
Eco88I CYCGRG 2 cut(s) 482, 495
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 1 cut(s) 209
FaiI YATR 7 cut(s) 134, 207, 656, 690, 699, 701, 726
FaqI GGGAC 2 cut(s) 299, 500
FatI CATG 1 cut(s) 205
FbaI TGATCA 1 cut(s) 727
FblI GTMKAC 1 cut(s) 249
Fnu4HI GCNGC 6 cut(s) 129, 210, 244, 261, 307, 474
FokI GGATG 2 cut(s) 320, 751
Fsp4HI GCNGC 6 cut(s) 129, 210, 244, 261, 307, 474
FspBI CTAG 2 cut(s) 21, 431
GlaI GCGC 5 cut(s) 12, 30, 185, 238, 760
GluI GCNGC 6 cut(s) 129, 210, 244, 261, 307, 474
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 6 cut(s) 269, 300, 378, 563, 626, 633
HapII CCGG 7 cut(s) 82, 116, 379, 441, 496, 540, 634
HgaI GACGC 4 cut(s) 166, 175, 233, 235
HhaI GCGC 5 cut(s) 13, 31, 186, 239, 761
Hin1I GRCGYC 2 cut(s) 225, 372
Hin1II CATG 1 cut(s) 209
Hin6I GCGC 5 cut(s) 11, 29, 184, 237, 759
HinP1I GCGC 5 cut(s) 11, 29, 184, 237, 759
HincII GTYRAC 1 cut(s) 250
HindII GTYRAC 1 cut(s) 250
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 4 cut(s) 41, 98, 466, 746
HpaII CCGG 7 cut(s) 82, 116, 379, 441, 496, 540, 634
HphI GGTGA 2 cut(s) 319, 602
Hpy166II GTNNAC 3 cut(s) 169, 250, 558
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 6 cut(s) 82, 195, 388, 576, 644, 750
Hpy8I GTNNAC 3 cut(s) 169, 250, 558
Hpy99I CGWCG 7 cut(s) 227, 251, 254, 359, 374, 377, 632
HpyCH4III ACNGT 4 cut(s) 394, 522, 599, 610
HpyCH4IV ACGT 3 cut(s) 324, 348, 372
HpyF10VI GCNNNNNNNGC 7 cut(s) 125, 183, 243, 266, 275, 306, 470
HpyF3I CTNAG 2 cut(s) 43, 525
HpySE526I ACGT 3 cut(s) 324, 348, 372
Hsp92I GRCGYC 2 cut(s) 225, 372
Hsp92II CATG 1 cut(s) 209
HspAI GCGC 5 cut(s) 11, 29, 184, 237, 759
Kpn2I TCCGGA 1 cut(s) 81
KroI GCCGGC 1 cut(s) 539
KroNI GCCGGC 1 cut(s) 541
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 2 cut(s) 691, 727
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 3 cut(s) 196, 230, 293
MaeI CTAG 2 cut(s) 21, 431
MaeII ACGT 3 cut(s) 324, 348, 372
MaeIII GTNAC 1 cut(s) 325
MalI GATC 2 cut(s) 693, 729
MbiI CCGCTC 1 cut(s) 192
MboI GATC 2 cut(s) 691, 727
MboII GAAGA 1 cut(s) 343
MlyI GAGTC 3 cut(s) 35, 92, 755
MmeI TCCRAC 1 cut(s) 174
MreI CGCCGGCG 1 cut(s) 539
MroI TCCGGA 1 cut(s) 81
MroNI GCCGGC 1 cut(s) 539
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 2 cut(s) 383, 675
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 7 cut(s) 82, 116, 379, 441, 496, 540, 634
MspR9I CCNGG 4 cut(s) 116, 496, 497, 669
MvaI CCWGG 1 cut(s) 669
MvnI CGCG 4 cut(s) 29, 186, 196, 239
MwoI GCNNNNNNNGC 7 cut(s) 125, 183, 243, 266, 275, 306, 470
NaeI GCCGGC 1 cut(s) 541
NciI CCSGG 3 cut(s) 116, 496, 497
NdeII GATC 2 cut(s) 691, 727
NgoMIV GCCGGC 1 cut(s) 539
NlaIII CATG 1 cut(s) 209
NlaIV GGNNCC 3 cut(s) 86, 516, 564
NmeAIII GCCGAG 2 cut(s) 15, 198
NmuCI GTSAC 1 cut(s) 325
NruI TCGCGA 1 cut(s) 196
NspV TTCGAA 1 cut(s) 162
OliI CACNNNNGTG 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PdiI GCCGGC 1 cut(s) 541
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 1 cut(s) 466
PkrI GCNGC 6 cut(s) 130, 211, 245, 262, 308, 475
PleI GAGTC 3 cut(s) 35, 92, 754
PpsI GAGTC 3 cut(s) 35, 92, 754
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspN4I GGNNCC 3 cut(s) 86, 516, 564
PspPI GGNCC 3 cut(s) 268, 514, 562
PvuII CAGCTG 1 cut(s) 306
RruI TCGCGA 1 cut(s) 196
RsaI GTAC 1 cut(s) 559
RsaNI GTAC 1 cut(s) 558
RseI CAYNNNNRTG 1 cut(s) 588
SalI GTCGAC 1 cut(s) 248
SaqAI TTAA 2 cut(s) 383, 675
SatI GCNGC 6 cut(s) 129, 210, 244, 261, 307, 474
Sau3AI GATC 2 cut(s) 691, 727
Sau96I GGNCC 3 cut(s) 268, 514, 562
SchI GAGTC 3 cut(s) 35, 92, 755
ScrFI CCNGG 4 cut(s) 116, 496, 497, 669
SfuI TTCGAA 1 cut(s) 162
SgrAI CRCCGGYG 1 cut(s) 539
SgrDI CGTCGACG 1 cut(s) 248
SinI GGWCC 1 cut(s) 514
SmaI CCCGGG 1 cut(s) 497
SmiMI CAYNNNNRTG 1 cut(s) 588
SsiI CCGC 7 cut(s) 73, 129, 190, 261, 473, 537, 588
SspMI CTAG 2 cut(s) 21, 431
StyD4I CCNGG 4 cut(s) 114, 494, 495, 667
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 4 cut(s) 394, 522, 599, 610
TaiI ACGT 3 cut(s) 327, 351, 375
TaqI TCGA 5 cut(s) 162, 249, 357, 369, 749
TauI GCSGC 3 cut(s) 131, 263, 476
TfiI GAWTC 1 cut(s) 466
Tru1I TTAA 2 cut(s) 383, 675
Tru9I TTAA 2 cut(s) 383, 675
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 3 cut(s) 209, 243, 306
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 629
TspMI CCCGGG 1 cut(s) 495
TspRI CASTG 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 514
XmaI CCCGGG 1 cut(s) 495
XmiI GTMKAC 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 21, 431
ZraI GACGTC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.