AT5G62070

IQ-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009886
Physical Location & Seq
Reverse (-)
24929859 .. 24932686
2828 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62070.1

Sequence Viewer

Length: 1212 bp
ATGGGCTTTTTCGGGAGACTGTTCGGGAGTAAAAAAAAGTCTGATAAAGCTGCTTCGTCGAGAGATAAACGGAGGTGGAGCTTCACCACCAGATCTTCAAATTCCAGCAAGAGAGCTCCGGCGGTGACGTCGGCTTCGGTGGTTGAGCAAAATGGTTTGGATGCTGACAAACATGCGATAGCTGTGGCTGCTGCGACAGCCGCGGTGGCGGAAGCAGCTCTTACTGCTGCTCATGCGGCAGCTGAAGTCGTGAGACTAACAAGCGGAAACGGCGGGAGAAACGTCGGTGGCGGTGGAAACTCGTCGGTGTTTCAAATAGGAAGAAGTAACCGTCGGTGGGCTCAGGAGAATATCGCGGCGATGAAGATTCAATCCGCTTTTCGTGGTTATCTGGCGAGGAGAGCATTACGAGCACTAAAGGCATTAGTGAAGCTTCAAGCATTAGTGAGAGGACACATAGTGAGAAAACAAACTGCAGATATGCTTAGAAGGATGCAGACTCTTGTTCGTCTTCAATCTCAAGCTCGTGCTCGAGCCTCTCGTTCTTCTCACTCCTCTGCTTCTTTCCACTCCTCCACCGCTCTTTTGTTCCCATCTTCCTCGTCTTCTCCACGTTCTCTTCACACGCGCTGCGTTTCAAACGCTGAAGTCAGCTCTCTTGACCACCGTGGAGGCTCTAAGCGGTTAGATTGGCAGGCGGAGGAAAGCGAAAATGGAGACAAGATCCTAGAAGTGGATACTTGGAAGCCTCATTATCATCCCAAACCGTTACGTTCAGAGAGAAACAATGAGTCTCCGAGGAAACGACAACAATCTTTGTTGGGTCCGAGAAGTACAGAGAATAGTCCTCAAGTTGGTTCTAGTGGGTCAAGAAGAAGAACTCCTTTTACGCCGACGTCAAGAAGCGAGTATTCTTGGGGATGTAATAACTATTACTACTCGGGTTATCACCCGAATTACATGGCTAACACTGAGTCTTATAAAGCTAAAGTTCGGTCACAAAGTGCGCCGAAACAGAGAGTTGAGGTCTCTAATGAGACCAGTGGCTACAAGAGATCTGTTCAGGGACAGTATTACTACTACACAGCGGTAGAAGAAGAGAGTTTGGATGTTGGAAGCGCTGGTTACTACGGAGGAGGAGGAGGCGATTCTGATCGATTGAATCGGAACCAAAGTGCGAAATCGAGGATGCATTCTTCGTTTCTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

403

Amino Acids

44.27

Weight (kDa)

10.48

Isoelectric Point (pI)

71.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IQ PF00612 119 - 137 5.5e-06 IQ calmodulin-binding motif
DUF4005 PF13178 281 - 399 1e-15 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 981
AatII GACGTC 2 cut(s) 131, 899
AccBSI CCGCTC 1 cut(s) 581
AccII CGCG 3 cut(s) 203, 356, 628
AclWI GGATC 1 cut(s) 718
AcsI RAATTY 1 cut(s) 100
AcuI CTGAAG 2 cut(s) 264, 666
AcyI GRCGYC 2 cut(s) 128, 896
AdeI CACNNNGTG 2 cut(s) 460, 1004
AfaI GTAC 1 cut(s) 835
AfeI AGCGCT 1 cut(s) 1120
AfiI CCNNNNNNNGG 3 cut(s) 337, 733, 854
AgsI TTSAA 7 cut(s) 99, 314, 371, 437, 515, 639, 1162
Alw21I GWGCWC 3 cut(s) 118, 415, 532
Alw26I GTCTC 6 cut(s) 10, 247, 711, 798, 1031, 1033
AlwI GGATC 1 cut(s) 718
Ama87I CYCGRG 2 cut(s) 531, 940
Aor51HI AGCGCT 1 cut(s) 1120
ApeKI GCWGC 7 cut(s) 50, 188, 191, 215, 227, 239, 630
ApoI RAATTY 1 cut(s) 100
ArsI GACNNNNNNTTYG 2 cut(s) 118, 150
AspLEI GCGC 3 cut(s) 630, 1009, 1121
AspS9I GGNCC 1 cut(s) 824
AsuHPI GGTGA 3 cut(s) 76, 136, 941
AvaI CYCGRG 2 cut(s) 531, 940
AvaII GGWCC 1 cut(s) 824
BanII GRGCYC 2 cut(s) 118, 343
BauI CACGAG 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 503, 597
Bbv12I GWGCWC 3 cut(s) 118, 415, 532
BbvI GCAGC 7 cut(s) 37, 175, 178, 214, 227, 251, 617
BccI CCATC 1 cut(s) 601
BceAI ACGGC 1 cut(s) 286
BciVI GTATCC 1 cut(s) 730
BcoDI GTCTC 6 cut(s) 10, 247, 711, 798, 1031, 1033
BfaI CTAG 2 cut(s) 728, 861
BfmI CTRYAG 1 cut(s) 474
BfoI RGCGCY 1 cut(s) 1122
BfuI GTATCC 1 cut(s) 730
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 2 cut(s) 92, 1055
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 2 cut(s) 531, 940
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 2 cut(s) 825, 1169
BmsI GCATC 3 cut(s) 151, 483, 1179
BpiI GAAGAC 2 cut(s) 503, 597
Bpu10I CCTNAGC 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 504, 834
Bsa29I ATCGAT 1 cut(s) 1156
BsaBI GATNNNNATC 1 cut(s) 1152
BsaHI GRCGYC 2 cut(s) 128, 896
BsaI GGTCTC 2 cut(s) 1031, 1033
BsaJI CCNNGG 3 cut(s) 201, 667, 797
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 733, 854
Bse1I ACTGG 1 cut(s) 1041
Bse8I GATNNNNATC 1 cut(s) 1152
BseCI ATCGAT 1 cut(s) 1156
BseDI CCNNGG 3 cut(s) 201, 667, 797
BseGI GGATG 6 cut(s) 166, 498, 757, 926, 1114, 1194
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 3 cut(s) 337, 733, 854
BseMII CTCAG 2 cut(s) 356, 963
BseNI ACTGG 1 cut(s) 1041
BseRI GAGGAG 6 cut(s) 412, 544, 562, 1149, 1152, 1155
BseXI GCAGC 7 cut(s) 37, 175, 178, 214, 227, 251, 617
Bsh1236I CGCG 3 cut(s) 203, 356, 628
BshVI ATCGAT 1 cut(s) 1156
BsiHKAI GWGCWC 3 cut(s) 118, 415, 532
BsiHKCI CYCGRG 2 cut(s) 531, 940
BsiSI CCGG 1 cut(s) 119
BslFI GGGAC 1 cut(s) 1080
BslI CCNNNNNNNGG 3 cut(s) 337, 733, 854
BsmAI GTCTC 6 cut(s) 10, 247, 711, 798, 1031, 1033
BsmFI GGGAC 1 cut(s) 1080
BsmI GAATGC 1 cut(s) 1192
Bso31I GGTCTC 2 cut(s) 1031, 1033
BsoBI CYCGRG 2 cut(s) 531, 940
Bsp1286I GDGCHC 4 cut(s) 118, 343, 415, 532
Bsp143I GATC 4 cut(s) 92, 723, 1055, 1153
BspCNI CTCAG 2 cut(s) 355, 964
BspDI ATCGAT 1 cut(s) 1156
BspFNI CGCG 3 cut(s) 203, 356, 628
BspLI GGNNCC 2 cut(s) 825, 1169
BspMAI CTGCAG 1 cut(s) 478
BspPI GGATC 1 cut(s) 718
BspTNI GGTCTC 2 cut(s) 1031, 1033
BsrBI CCGCTC 1 cut(s) 581
BsrI ACTGG 1 cut(s) 1041
BssECI CCNNGG 3 cut(s) 201, 667, 797
BssMI GATC 4 cut(s) 92, 723, 1055, 1153
BssNI GRCGYC 2 cut(s) 128, 896
BssSI CACGAG 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 525
Bst4CI ACNGT 5 cut(s) 21, 332, 668, 768, 1071
Bst6I CTCTTC 2 cut(s) 624, 1092
BstACI GRCGYC 2 cut(s) 128, 896
BstC8I GCNNGC 1 cut(s) 696
BstDEI CTNAG 4 cut(s) 342, 485, 678, 972
BstDSI CCRYGG 2 cut(s) 201, 667
BstF5I GGATG 6 cut(s) 166, 498, 757, 926, 1114, 1194
BstFNI CGCG 3 cut(s) 203, 356, 628
BstH2I RGCGCY 1 cut(s) 1122
BstHHI GCGC 3 cut(s) 630, 1009, 1121
BstKTI GATC 4 cut(s) 95, 726, 1058, 1156
BstMAI GTCTC 6 cut(s) 10, 247, 711, 798, 1031, 1033
BstMBI GATC 4 cut(s) 92, 723, 1055, 1153
BstNSI RCATGY 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 474
BstUI CGCG 3 cut(s) 203, 356, 628
BstV1I GCAGC 7 cut(s) 37, 175, 178, 214, 227, 251, 617
BstV2I GAAGAC 2 cut(s) 503, 597
BstX2I RGATCY 3 cut(s) 92, 723, 1055
BstYI RGATCY 3 cut(s) 92, 723, 1055
Bsu15I ATCGAT 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 730
BsuTUI ATCGAT 1 cut(s) 1156
BtgI CCRYGG 2 cut(s) 201, 667
BtgZI GCGATG 1 cut(s) 374
BtsCI GGATG 6 cut(s) 166, 498, 757, 926, 1114, 1194
BtsIMutI CAGTG 2 cut(s) 969, 1048
Cac8I GCNNGC 1 cut(s) 696
CfoI GCGC 3 cut(s) 630, 1009, 1121
Cfr13I GGNCC 1 cut(s) 824
Cfr42I CCGCGG 1 cut(s) 204
ClaI ATCGAT 1 cut(s) 1156
Csp6I GTAC 1 cut(s) 834
CviAII CATG 3 cut(s) 173, 233, 961
CviQI GTAC 1 cut(s) 834
DdeI CTNAG 4 cut(s) 342, 485, 678, 972
DpnI GATC 4 cut(s) 94, 725, 1057, 1155
DpnII GATC 4 cut(s) 92, 723, 1055, 1153
DraIII CACNNNGTG 2 cut(s) 460, 1004
Eam1104I CTCTTC 2 cut(s) 624, 1092
EarI CTCTTC 2 cut(s) 624, 1092
EciI GGCGGA 2 cut(s) 224, 713
Ecl136II GAGCTC 1 cut(s) 116
Eco24I GRGCYC 2 cut(s) 118, 343
Eco31I GGTCTC 2 cut(s) 1031, 1033
Eco47I GGWCC 1 cut(s) 824
Eco47III AGCGCT 1 cut(s) 1120
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 2 cut(s) 264, 666
Eco88I CYCGRG 2 cut(s) 531, 940
EcoICRI GAGCTC 1 cut(s) 116
EcoT22I ATGCAT 1 cut(s) 1194
EcoT38I GRGCYC 2 cut(s) 118, 343
FaeI CATG 3 cut(s) 176, 236, 964
FaiI YATR 6 cut(s) 174, 234, 458, 482, 962, 981
FalI AAGNNNNNCTT 4 cut(s) 204, 236, 868, 900
FaqI GGGAC 1 cut(s) 1080
FatI CATG 3 cut(s) 172, 232, 960
FauI CCCGC 1 cut(s) 266
FokI GGATG 6 cut(s) 173, 505, 744, 933, 1121, 1201
FriOI GRGCYC 2 cut(s) 118, 343
FspBI CTAG 2 cut(s) 728, 861
GlaI GCGC 3 cut(s) 629, 1008, 1120
HaeII RGCGCY 1 cut(s) 1122
HapII CCGG 1 cut(s) 119
HhaI GCGC 3 cut(s) 630, 1009, 1121
Hin1I GRCGYC 2 cut(s) 128, 896
Hin1II CATG 3 cut(s) 176, 236, 964
Hin6I GCGC 3 cut(s) 628, 1007, 1119
HinP1I GCGC 3 cut(s) 628, 1007, 1119
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 6 cut(s) 367, 499, 791, 974, 1148, 1162
HpaII CCGG 1 cut(s) 119
HphI GGTGA 3 cut(s) 76, 136, 941
Hpy188I TCNGA 6 cut(s) 43, 778, 798, 828, 1153, 1167
Hpy188III TCNNGA 8 cut(s) 13, 25, 60, 250, 344, 659, 870, 900
Hpy99I CGWCG 6 cut(s) 61, 133, 287, 307, 336, 898
HpyAV CCTTC 1 cut(s) 483
HpyCH4III ACNGT 5 cut(s) 21, 332, 668, 768, 1071
HpyCH4IV ACGT 5 cut(s) 128, 282, 613, 772, 896
HpyCH4V TGCA 3 cut(s) 476, 496, 1192
HpyF3I CTNAG 4 cut(s) 342, 485, 678, 972
HpySE526I ACGT 5 cut(s) 128, 282, 613, 772, 896
Hsp92I GRCGYC 2 cut(s) 128, 896
Hsp92II CATG 3 cut(s) 176, 236, 964
HspAI GCGC 3 cut(s) 628, 1007, 1119
KspI CCGCGG 1 cut(s) 204
Kzo9I GATC 4 cut(s) 92, 723, 1055, 1153
LmnI GCTCC 2 cut(s) 78, 121
LpnPI CCDG 9 cut(s) 103, 118, 132, 329, 377, 680, 1049, 1054, 1107
Lsp1109I GCAGC 7 cut(s) 37, 175, 178, 214, 227, 251, 617
LweI GCATC 3 cut(s) 151, 483, 1179
MaeI CTAG 2 cut(s) 728, 861
MaeII ACGT 5 cut(s) 128, 282, 613, 772, 896
MaeIII GTNAC 5 cut(s) 124, 326, 768, 996, 1124
MalI GATC 4 cut(s) 94, 725, 1057, 1155
MbiI CCGCTC 1 cut(s) 581
MboI GATC 4 cut(s) 92, 723, 1055, 1153
MflI RGATCY 3 cut(s) 92, 723, 1055
MhlI GDGCHC 4 cut(s) 118, 343, 415, 532
MluCI AATT 2 cut(s) 100, 955
MlyI GAGTC 3 cut(s) 493, 800, 983
MmeI TCCRAC 1 cut(s) 1093
Mph1103I ATGCAT 1 cut(s) 1194
MspA1I CMGCKG 3 cut(s) 203, 242, 1088
MspI CCGG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 1192
MvnI CGCG 3 cut(s) 203, 356, 628
NdeII GATC 4 cut(s) 92, 723, 1055, 1153
NlaIII CATG 3 cut(s) 176, 236, 964
NlaIV GGNNCC 2 cut(s) 825, 1169
NmuCI GTSAC 2 cut(s) 124, 996
NsiI ATGCAT 1 cut(s) 1194
NspI RCATGY 1 cut(s) 176
PaeR7I CTCGAG 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 538
PctI GAATGC 1 cut(s) 1192
PfeI GAWTC 3 cut(s) 367, 1148, 1162
PleI GAGTC 3 cut(s) 493, 799, 982
PpsI GAGTC 3 cut(s) 493, 799, 982
PsiI TTATAA 1 cut(s) 981
Psp124BI GAGCTC 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 825, 1169
PspPI GGNCC 1 cut(s) 824
PspXI VCTCGAGB 1 cut(s) 531
PsrI GAACNNNNNNTAC 2 cut(s) 871, 903
PstI CTGCAG 1 cut(s) 478
PsuI RGATCY 3 cut(s) 92, 723, 1055
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 1 cut(s) 835
RsaNI GTAC 1 cut(s) 834
SacI GAGCTC 1 cut(s) 118
SacII CCGCGG 1 cut(s) 204
Sau3AI GATC 4 cut(s) 92, 723, 1055, 1153
Sau96I GGNCC 1 cut(s) 824
SchI GAGTC 3 cut(s) 493, 800, 983
SduI GDGCHC 4 cut(s) 118, 343, 415, 532
SfaNI GCATC 3 cut(s) 151, 483, 1179
SfcI CTRYAG 1 cut(s) 474
Sfr274I CTCGAG 1 cut(s) 531
Sfr303I CCGCGG 1 cut(s) 204
SgrBI CCGCGG 1 cut(s) 204
SinI GGWCC 1 cut(s) 824
SlaI CTCGAG 1 cut(s) 531
SmlI CTYRAG 3 cut(s) 519, 531, 849
SmoI CTYRAG 3 cut(s) 519, 531, 849
Sse9I AATT 2 cut(s) 100, 955
SspMI CTAG 2 cut(s) 728, 861
SstI GAGCTC 1 cut(s) 118
TaaI ACNGT 5 cut(s) 21, 332, 668, 768, 1071
TaiI ACGT 5 cut(s) 131, 285, 616, 775, 899
TaqI TCGA 4 cut(s) 59, 532, 1156, 1184
TaqII GACCGA 1 cut(s) 984
TasI AATT 2 cut(s) 100, 955
TatI WGTACW 1 cut(s) 833
TauI GCSGC 3 cut(s) 203, 239, 359
TfiI GAWTC 3 cut(s) 367, 1148, 1162
TscAI CASTG 2 cut(s) 976, 1048
TseFI GTSAC 2 cut(s) 124, 996
TseI GCWGC 7 cut(s) 50, 188, 191, 215, 227, 239, 630
Tsp45I GTSAC 2 cut(s) 124, 996
TspDTI ATGAA 1 cut(s) 377
TspGWI ACGGA 2 cut(s) 85, 1146
TspRI CASTG 2 cut(s) 976, 1048
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 1 cut(s) 100
XceI RCATGY 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 531
XspI CTAG 2 cut(s) 728, 861
ZraI GACGTC 2 cut(s) 129, 897
Zsp2I ATGCAT 1 cut(s) 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.