FvH4_7g29094

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009886
Physical Location & Seq
Forward (+)
21515658 .. 21518384
2727 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29094.t1

Sequence Viewer

Length: 1239 bp
ATGGACGCCAACAAGCACGCCATAGCGGTAGCCGCAGCTACTGCCGCCGTGGCCGAGGCAGCTCTCGCGGCGGCTCATGCGGCGGCGGAGGTTGTCAGGCTCACTAACGGCGTTGGGACTTCCGGGAACAACGTTAGCTTACCTGTTAACGTCCGCCGTCACCGCAACCTCGCCGCCGTTAAGATACAGTCAGCTTTCCGGAGATACCTGGCAAGGAGGGCTTTAAGGGCACTGAAAGCACTGGTGAAACTTCAAGCATTGGTGAGAGGCCACATAGTAAGGAAACAAACGGCAGACATGCTCAAGCGCATGCAGACACTGGTTCGACTCCAGGACCGTGCACGGGCAACTCGCTCACTCATGTCAGAGTCAGTACATTCTAGCAGCAAATCCTCCCTGTCTTACAATCCTCTCCCAGAAAGTCCTGACAAAATTGGATACCAGCAACGTGTTTATAGTAGCAAATTTGATGGGCCCTCTTTGCTGAAGAGGTGTGGTTCAAATTCAAAGGTGAGAAATACCACCAGTCTGGACAGAAGACGTCTGGCCTCATCAGGATGGTTAGATCGGTGGATGGAAGAAAGTGTATGGAACAACAGCCGTGATGCTTCATCACTGAGATATGTGCAAGCAGATAGTGAGAAGGTTGACAAAATCCTTGAAGTAGATACTTGGAAACCTCACTTGGGCTCCCAACGAAATGCCAGAGCTTTTCAAACCGCACAGCATGTTATGGTTTCGGATAATAATAGTCCATTGTTTATGACATTTGATTCCCCGTCCAAGCGCACAACAAAAGAACCAAGTTTGATTCCAAGTCAATCGTCCATGGATTACTACTCATCAAACTCTTTGAAATATCCTATAGGAAAAGATGAAGTAGTGGCTTCAAGAACTGCAGAGAACAGCCCACAAGCATTTTCTGCCTCATCGAGACCTGGAAGTAGGAGTGCTAGAAGAGCCCACTTCTCGCCAACAAGGAGCGAGTACTCGTGGGGTTTCTTTAGCAGCTATGCCGGTTACCCTAGCTACATGGCTAACACGGAATCATCCCGGGCTAAGGTTCGGTCACAGAGTGCTCCAAGACAAAGGCTTGAATTTGAGAAATATAGTTTGACAAAAAAATCCATCCAAGAGTTTTGTGAAGCAGGCACATGTTCAGAAACAGGTTTAGCTCAAGATGCTGATTTCAGGACTAAAGCCTTTCTGAGCTCTAGCCACTCAAACAGGATCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

413

Amino Acids

45.82

Weight (kDa)

10.32

Isoelectric Point (pI)

60.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 315 - 366 8.6e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 544
AccII CGCG 1 cut(s) 68
AccIII TCCGGA 1 cut(s) 198
AclI AACGTT 1 cut(s) 132
AclWI GGATC 2 cut(s) 1225, 1238
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 3 cut(s) 464, 502, 1097
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 2 cut(s) 6, 541
AdeI CACNNNGTG 1 cut(s) 1076
AfaI GTAC 2 cut(s) 375, 989
AfiI CCNNNNNNNGG 3 cut(s) 343, 686, 1060
AflIII ACRYGT 2 cut(s) 448, 1154
AgsI TTSAA 8 cut(s) 254, 501, 507, 662, 716, 856, 891, 1097
AjnI CCWGG 3 cut(s) 207, 330, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 668, 700
AluBI AGCT 9 cut(s) 38, 62, 138, 194, 710, 1011, 1029, 1175, 1212
AluI AGCT 9 cut(s) 38, 62, 138, 194, 710, 1011, 1029, 1175, 1212
Alw21I GWGCWC 3 cut(s) 343, 1081, 1214
Alw26I GTCTC 1 cut(s) 928
Alw44I GTGCAC 1 cut(s) 339
AlwI GGATC 2 cut(s) 1225, 1238
AlwNI CAGNNNCTG 2 cut(s) 41, 319
Ama87I CYCGRG 1 cut(s) 1053
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 4 cut(s) 51, 268, 473, 546
ApaI GGGCCC 1 cut(s) 477
ApaLI GTGCAC 1 cut(s) 339
ApeKI GCWGC 4 cut(s) 35, 59, 384, 1008
ApoI RAATTY 3 cut(s) 464, 502, 1097
AspLEI GCGC 2 cut(s) 309, 789
AspS9I GGNCC 3 cut(s) 334, 473, 474
AsuC2I CCSGG 3 cut(s) 124, 1054, 1055
AsuHPI GGTGA 4 cut(s) 152, 256, 274, 523
AvaI CYCGRG 1 cut(s) 1053
AvaII GGWCC 1 cut(s) 334
BaeGI GKGCMC 3 cut(s) 232, 343, 477
BamHI GGATCC 1 cut(s) 1230
BanII GRGCYC 4 cut(s) 477, 692, 964, 1214
BauI CACGAG 1 cut(s) 991
BbsI GAAGAC 1 cut(s) 544
Bbv12I GWGCWC 3 cut(s) 343, 1081, 1214
BbvI GCAGC 4 cut(s) 47, 71, 396, 1020
BccI CCATC 4 cut(s) 464, 552, 568, 1136
BceAI ACGGC 6 cut(s) 32, 124, 141, 161, 306, 585
BciT130I CCWGG 3 cut(s) 209, 332, 939
BciVI GTATCC 1 cut(s) 431
BcnI CCSGG 3 cut(s) 124, 1054, 1055
BcoDI GTCTC 1 cut(s) 928
BfaI CTAG 4 cut(s) 381, 954, 1026, 1215
BfmI CTRYAG 2 cut(s) 864, 897
BfuI GTATCC 1 cut(s) 431
BglI GCCNNNNNGGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 989
Bme1390I CCNGG 6 cut(s) 124, 209, 332, 939, 1054, 1055
Bme18I GGWCC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 1053
BmgT120I GGNCC 3 cut(s) 334, 473, 474
BmiI GGNNCC 3 cut(s) 475, 691, 1232
BmrFI CCNGG 6 cut(s) 124, 209, 332, 939, 1054, 1055
BmsI GCATC 2 cut(s) 595, 1171
BpiI GAAGAC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 314
Bpu10I CCTNAGC 1 cut(s) 1059
BpuEI CTTGAG 2 cut(s) 287, 1161
BpuMI CCSGG 3 cut(s) 124, 1054, 1055
BsaHI GRCGYC 2 cut(s) 6, 541
BsaI GGTCTC 1 cut(s) 928
BsaJI CCNNGG 4 cut(s) 48, 54, 828, 1053
BsaWI WCCGGW 2 cut(s) 198, 1233
BsaXI ACNNNNNCTCC 2 cut(s) 674, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 686, 1060
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 3 cut(s) 246, 324, 525
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 3 cut(s) 209, 332, 939
BseDI CCNNGG 4 cut(s) 48, 54, 828, 1053
BseGI GGATG 4 cut(s) 563, 579, 1049, 1128
BseLI CCNNNNNNNGG 3 cut(s) 343, 686, 1060
BseMII CTCAG 2 cut(s) 608, 1199
BseNI ACTGG 3 cut(s) 246, 324, 525
BseSI GKGCMC 3 cut(s) 232, 343, 477
BseXI GCAGC 4 cut(s) 47, 71, 396, 1020
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 4 cut(s) 53, 270, 475, 548
BsiHKAI GWGCWC 3 cut(s) 343, 1081, 1214
BsiHKCI CYCGRG 1 cut(s) 1053
BsiSI CCGG 5 cut(s) 123, 199, 1017, 1054, 1234
BslFI GGGAC 1 cut(s) 130
BslI CCNNNNNNNGG 3 cut(s) 343, 686, 1060
BsmAI GTCTC 1 cut(s) 928
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 4 cut(s) 53, 270, 475, 548
Bso31I GGTCTC 1 cut(s) 928
BsoBI CYCGRG 1 cut(s) 1053
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 7 cut(s) 232, 343, 477, 692, 964, 1081, 1214
Bsp13I TCCGGA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 565, 1230
Bsp19I CCATGG 1 cut(s) 828
BspANI GGCC 4 cut(s) 53, 270, 475, 548
BspCNI CTCAG 2 cut(s) 609, 1200
BspEI TCCGGA 1 cut(s) 198
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 475, 691, 1232
BspMAI CTGCAG 1 cut(s) 901
BspPI GGATC 2 cut(s) 1225, 1238
BspQI GCTCTTC 1 cut(s) 952
BspTNI GGTCTC 1 cut(s) 928
BsrFI RCCGGY 1 cut(s) 1016
BsrI ACTGG 3 cut(s) 246, 324, 525
BssAI RCCGGY 1 cut(s) 1016
BssECI CCNNGG 4 cut(s) 48, 54, 828, 1053
BssMI GATC 2 cut(s) 565, 1230
BssNI GRCGYC 2 cut(s) 6, 541
BssSI CACGAG 1 cut(s) 991
BssT1I CCWWGG 1 cut(s) 828
Bst2BI CACGAG 1 cut(s) 991
Bst2UI CCWGG 3 cut(s) 209, 332, 939
Bst4CI ACNGT 2 cut(s) 189, 338
Bst6I CTCTTC 2 cut(s) 482, 952
BstACI GRCGYC 2 cut(s) 6, 541
BstAPI GCANNNNNTGC 2 cut(s) 41, 923
BstC8I GCNNGC 4 cut(s) 18, 311, 630, 1150
BstDEI CTNAG 3 cut(s) 617, 1059, 1208
BstDSI CCRYGG 2 cut(s) 48, 828
BstEII GGTNACC 1 cut(s) 1019
BstF5I GGATG 4 cut(s) 563, 579, 1049, 1128
BstFNI CGCG 1 cut(s) 68
BstHHI GCGC 2 cut(s) 309, 789
BstKTI GATC 2 cut(s) 568, 1233
BstMAI GTCTC 1 cut(s) 928
BstMBI GATC 2 cut(s) 565, 1230
BstNI CCWGG 3 cut(s) 209, 332, 939
BstNSI RCATGY 4 cut(s) 301, 313, 731, 1158
BstPI GGTNACC 1 cut(s) 1019
BstSCI CCNGG 6 cut(s) 122, 207, 330, 937, 1052, 1053
BstSFI CTRYAG 2 cut(s) 864, 897
BstSLI GKGCMC 3 cut(s) 232, 343, 477
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 4 cut(s) 47, 71, 396, 1020
BstV2I GAAGAC 1 cut(s) 544
BstX2I RGATCY 1 cut(s) 1230
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 1 cut(s) 1230
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 4 cut(s) 53, 270, 475, 548
BtgI CCRYGG 2 cut(s) 48, 828
BtsCI GGATG 4 cut(s) 563, 579, 1049, 1128
BtsIMutI CAGTG 4 cut(s) 230, 239, 317, 614
Cac8I GCNNGC 4 cut(s) 18, 311, 630, 1150
CaiI CAGNNNCTG 2 cut(s) 41, 319
CfoI GCGC 2 cut(s) 309, 789
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 3 cut(s) 334, 473, 474
Cfr9I CCCGGG 1 cut(s) 1053
CseI GACGC 1 cut(s) 14
Csp6I GTAC 2 cut(s) 374, 988
CviAII CATG 8 cut(s) 77, 298, 310, 361, 728, 829, 1033, 1155
CviQI GTAC 2 cut(s) 374, 988
DdeI CTNAG 3 cut(s) 617, 1059, 1208
DpnI GATC 2 cut(s) 567, 1232
DpnII GATC 2 cut(s) 565, 1230
DraIII CACNNNGTG 1 cut(s) 1076
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 482, 952
EarI CTCTTC 2 cut(s) 482, 952
EciI GGCGGA 2 cut(s) 101, 143
Ecl136II GAGCTC 1 cut(s) 1212
Eco130I CCWWGG 1 cut(s) 828
Eco24I GRGCYC 4 cut(s) 477, 692, 964, 1214
Eco31I GGTCTC 1 cut(s) 928
Eco47I GGWCC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 1212
Eco57I CTGAAG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 1053
Eco91I GGTNACC 1 cut(s) 1019
EcoICRI GAGCTC 1 cut(s) 1212
EcoO109I RGGNCCY 1 cut(s) 474
EcoO65I GGTNACC 1 cut(s) 1019
EcoRII CCWGG 3 cut(s) 207, 330, 937
EcoT14I CCWWGG 1 cut(s) 828
EcoT38I GRGCYC 4 cut(s) 477, 692, 964, 1214
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 8 cut(s) 80, 301, 313, 364, 731, 832, 1036, 1158
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FaqI GGGAC 1 cut(s) 130
FatI CATG 8 cut(s) 76, 297, 309, 360, 727, 828, 1032, 1154
FokI GGATG 4 cut(s) 570, 586, 1036, 1115
FriOI GRGCYC 4 cut(s) 477, 692, 964, 1214
FspBI CTAG 4 cut(s) 381, 954, 1026, 1215
GlaI GCGC 2 cut(s) 308, 788
GsuI CTGGAG 1 cut(s) 314
HaeIII GGCC 4 cut(s) 53, 270, 475, 548
HapII CCGG 5 cut(s) 123, 199, 1017, 1054, 1234
HgaI GACGC 1 cut(s) 14
HhaI GCGC 2 cut(s) 309, 789
Hin1I GRCGYC 2 cut(s) 6, 541
Hin1II CATG 8 cut(s) 80, 301, 313, 364, 731, 832, 1036, 1158
Hin6I GCGC 2 cut(s) 307, 787
HinP1I GCGC 2 cut(s) 307, 787
HincII GTYRAC 2 cut(s) 148, 649
HindII GTYRAC 2 cut(s) 148, 649
HinfI GANTC 5 cut(s) 327, 368, 773, 811, 1046
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 5 cut(s) 123, 199, 1017, 1054, 1234
HphI GGTGA 4 cut(s) 152, 256, 274, 523
Hpy166II GTNNAC 3 cut(s) 148, 341, 649
Hpy188I TCNGA 4 cut(s) 367, 742, 1162, 1209
Hpy188III TCNNGA 8 cut(s) 199, 425, 530, 555, 891, 933, 1178, 1192
Hpy8I GTNNAC 3 cut(s) 148, 341, 649
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 2 cut(s) 189, 338
HpyCH4IV ACGT 4 cut(s) 132, 150, 448, 541
HpyCH4V TGCA 4 cut(s) 313, 341, 628, 899
HpyF3I CTNAG 3 cut(s) 617, 1059, 1208
HpySE526I ACGT 4 cut(s) 132, 150, 448, 541
Hsp92I GRCGYC 2 cut(s) 6, 541
Hsp92II CATG 8 cut(s) 80, 301, 313, 364, 731, 832, 1036, 1158
HspAI GCGC 2 cut(s) 307, 787
Kpn2I TCCGGA 1 cut(s) 198
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 2 cut(s) 565, 1230
LguI GCTCTTC 1 cut(s) 952
LmnI GCTCC 3 cut(s) 695, 981, 1084
Lsp1109I GCAGC 4 cut(s) 47, 71, 396, 1020
LweI GCATC 2 cut(s) 595, 1171
MaeI CTAG 4 cut(s) 381, 954, 1026, 1215
MaeII ACGT 4 cut(s) 132, 150, 448, 541
MaeIII GTNAC 3 cut(s) 158, 1019, 1068
MalI GATC 2 cut(s) 567, 1232
MboI GATC 2 cut(s) 565, 1230
MboII GAAGA 4 cut(s) 499, 549, 590, 969
MflI RGATCY 1 cut(s) 1230
MhlI GDGCHC 7 cut(s) 232, 343, 477, 692, 964, 1081, 1214
MluCI AATT 4 cut(s) 432, 464, 502, 1097
MlyI GAGTC 2 cut(s) 321, 377
MroI TCCGGA 1 cut(s) 198
MseI TTAA 3 cut(s) 147, 180, 224
MslI CAYNNNNRTG 1 cut(s) 556
MspI CCGG 5 cut(s) 123, 199, 1017, 1054, 1234
MspR9I CCNGG 6 cut(s) 124, 209, 332, 939, 1054, 1055
MvaI CCWGG 3 cut(s) 209, 332, 939
MvnI CGCG 1 cut(s) 68
NciI CCSGG 3 cut(s) 124, 1054, 1055
NcoI CCATGG 1 cut(s) 828
NdeII GATC 2 cut(s) 565, 1230
NlaIII CATG 8 cut(s) 80, 301, 313, 364, 731, 832, 1036, 1158
NlaIV GGNNCC 3 cut(s) 475, 691, 1232
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 2 cut(s) 158, 1068
NspI RCATGY 4 cut(s) 301, 313, 731, 1158
PaeI GCATGC 1 cut(s) 313
PciI ACATGT 1 cut(s) 1154
PciSI GCTCTTC 1 cut(s) 952
PfeI GAWTC 3 cut(s) 773, 811, 1046
PfoI TCCNGGA 2 cut(s) 122, 330
PleI GAGTC 2 cut(s) 321, 376
PpsI GAGTC 2 cut(s) 321, 376
PscI ACATGT 1 cut(s) 1154
Psp124BI GAGCTC 1 cut(s) 1214
Psp1406I AACGTT 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 207, 330, 937
PspEI GGTNACC 1 cut(s) 1019
PspGI CCWGG 3 cut(s) 207, 330, 937
PspN4I GGNNCC 3 cut(s) 475, 691, 1232
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 3 cut(s) 334, 473, 474
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 2 cut(s) 41, 319
PsuI RGATCY 1 cut(s) 1230
RsaI GTAC 2 cut(s) 375, 989
RsaNI GTAC 2 cut(s) 374, 988
RseI CAYNNNNRTG 1 cut(s) 556
SacI GAGCTC 1 cut(s) 1214
SapI GCTCTTC 1 cut(s) 952
SaqAI TTAA 3 cut(s) 147, 180, 224
Sau3AI GATC 2 cut(s) 565, 1230
Sau96I GGNCC 3 cut(s) 334, 473, 474
ScaI AGTACT 1 cut(s) 989
SchI GAGTC 2 cut(s) 321, 377
ScrFI CCNGG 6 cut(s) 124, 209, 332, 939, 1054, 1055
SduI GDGCHC 7 cut(s) 232, 343, 477, 692, 964, 1081, 1214
SfaNI GCATC 2 cut(s) 595, 1171
SfcI CTRYAG 2 cut(s) 864, 897
SinI GGWCC 1 cut(s) 334
SmaI CCCGGG 1 cut(s) 1055
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 2 cut(s) 302, 1176
SmoI CTYRAG 2 cut(s) 302, 1176
SphI GCATGC 1 cut(s) 313
Sse9I AATT 4 cut(s) 432, 464, 502, 1097
SspMI CTAG 4 cut(s) 381, 954, 1026, 1215
SstI GAGCTC 1 cut(s) 1214
StyD4I CCNGG 6 cut(s) 122, 207, 330, 937, 1052, 1053
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 2 cut(s) 189, 338
TaiI ACGT 4 cut(s) 135, 153, 451, 544
TaqI TCGA 2 cut(s) 325, 932
TaqII GACCGA 1 cut(s) 1056
TasI AATT 4 cut(s) 432, 464, 502, 1097
TatI WGTACW 2 cut(s) 373, 987
TauI GCSGC 7 cut(s) 35, 47, 71, 74, 83, 86, 176
TfiI GAWTC 3 cut(s) 773, 811, 1046
Tru1I TTAA 3 cut(s) 147, 180, 224
Tru9I TTAA 3 cut(s) 147, 180, 224
TscAI CASTG 4 cut(s) 237, 246, 324, 621
TseFI GTSAC 2 cut(s) 158, 1068
TseI GCWGC 4 cut(s) 35, 59, 384, 1008
Tsp45I GTSAC 2 cut(s) 158, 1068
TspDTI ATGAA 2 cut(s) 600, 891
TspGWI ACGGA 1 cut(s) 1058
TspMI CCCGGG 1 cut(s) 1053
TspRI CASTG 4 cut(s) 237, 246, 324, 621
VneI GTGCAC 1 cut(s) 339
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 3 cut(s) 464, 502, 1097
XceI RCATGY 4 cut(s) 301, 313, 731, 1158
XmaI CCCGGG 1 cut(s) 1053
XspI CTAG 4 cut(s) 381, 954, 1026, 1215
ZraI GACGTC 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.