MD01G1197700.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009886
Physical Location & Seq
Forward (+)
29578375 .. 29582838
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1197700.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGGGCTTTTTCGGGCGACTCTTCGGCTGCAAACGCTTCAAAAACAACCCTTATCCGACCACCAAGCACACCAACAAGGAAAAGAGTCAAAAGACTACTACTTCTGGGTCTAACAAGCACAAGCTCGGTGCCTCCGCGTCAGCAGATGCTGCTCAAGGATGGGCGGACCTGGACGCCAACAAGCACGCGATAGCTGTAGCCGCCGCTACGGCAGCCGTCGCTGAGGCTGCTCTCGCGGCGGCTCATGCGGCGGCTGAGGTCGTTCGCCTCACTAATGGTGCTCCACCGGGGACCTCCTCGCAAGTTAGCCTGCCCGGGGCGGCGCCTTCAATCGTCCGACGACATCTCGCAGCCGTGAAGATACAGTCGGCTTTCCGGCGATACCTGGCAAGAAGGGCCCTGAGAGCACTCAAATCACTGGTGAAGCTTCAAGCTTTGGTGAGAGGCCGCATTGTGAGAAAGCAATCGGCAGACATGCTCAGGCGTATGCAGACACTGGTCCGAGTCCAAGCCCGAGCCCGTGCTAGTCAATCACTCATGCATTCTAGCAGTAAATCCTCCCTATCGGATCACCCGCCAGAAAGCCCTGACAAAACTGGATACCAGCACCGCGTTTATAGCGGCAAATTTGACGGGCCCTCAATACTGCAGAGATGCCGTTCAAATTCAAACGTAAGGGACGCCACAAATCTAGACAGAAGACGGCTGGCATCAGGTTGGTTAGACCAGTGGATGGAAGAAGGTACATGGGACAACCGCCGAGATGGTTCGCTGAGATATGAGCACTCAGATGATGTGAAGGTTGACAAAATTCTTGAAGTAGATACTTGGAAGCCTCACTTGGGTTCCCAACGTAGACCCCCAGCCTTTCAAACAGCACATCGCGTTGTGTCTTCAGATCATTACAACCCAAACTTTATGACATATGACTCTCCATCAAAACGCTCGACAAAAGGATCAAACCTCAATCCAAGTCAAGCTTCTATCAACGCTGTTTCAATGCGATATCTGAAATATCCCTTGGCAACGGATGAAGCAGCTTTTAGGACCGCCCCGAACAGCCCACAAGCATTTTCTGCCTCCTGTAGACCTGGAAGCAGTGGTAGAAGAGGTCATCACTTCACGCCAGCAAGGAGTGAGTGCTCGTGGGGCTTCTTTAATGGGTATGCCAGTCACCCTAACTACATGGCTAACACGGAATCATCCCGAGCTAAGGTCAGGTCACAAAGTGCCCCTAGGCAAAGACTTGAGTTTGAGAAATATGGTTTGACCAAGAAGTCTGTTCCGGAGTCTTGGAATGCAGGAACTCATTCAGATACGAGCTTCGCTGAAGATGGTGACCTCCGAAATAAAGCCTTTTCCAGCTCCAGCCGCCTGAATAGAGTTGGAAGCTCCAATCTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

469

Amino Acids

51.6

Weight (kDa)

10.5

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 370 - 434 4.5e-12 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1276
AccB1I GGYRCC 2 cut(s) 128, 322
AccB7I CCANNNNNTGG 1 cut(s) 733
AccI GTMKAC 2 cut(s) 856, 1087
AccII CGCG 5 cut(s) 137, 188, 236, 612, 885
AccIII TCCGGA 1 cut(s) 1285
AclWI GGATC 2 cut(s) 576, 964
AcsI RAATTY 3 cut(s) 626, 664, 810
AcuI CTGAAG 2 cut(s) 879, 1350
AcyI GRCGYC 3 cut(s) 174, 323, 681
AdeI CACNNNGTG 1 cut(s) 1229
AfaI GTAC 1 cut(s) 745
AfiI CCNNNNNNNGG 5 cut(s) 316, 733, 842, 1213, 1401
AgsI TTSAA 8 cut(s) 40, 330, 431, 663, 669, 818, 872, 999
AjnI CCWGG 3 cut(s) 168, 384, 1090
AjuI GAANNNNNNNTTGG 6 cut(s) 824, 856, 964, 996, 1004, 1036
Alw21I GWGCWC 4 cut(s) 283, 409, 786, 1145
AlwI GGATC 2 cut(s) 576, 964
AlwNI CAGNNNCTG 2 cut(s) 149, 496
Ama87I CYCGRG 3 cut(s) 314, 513, 1206
Aor13HI TCCGGA 1 cut(s) 1285
AoxI GGCC 3 cut(s) 396, 445, 635
ApaI GGGCCC 2 cut(s) 400, 639
ApeKI GCWGC 6 cut(s) 27, 149, 212, 227, 350, 1037
ApoI RAATTY 3 cut(s) 626, 664, 810
Asp700I GAANNNNTTC 1 cut(s) 1309
AspA2I CCTAGG 1 cut(s) 1235
AspLEI GCGC 1 cut(s) 325
AspS9I GGNCC 8 cut(s) 166, 291, 396, 397, 499, 635, 636, 1047
AsuC2I CCSGG 3 cut(s) 288, 315, 316
AsuHPI GGTGA 5 cut(s) 433, 451, 563, 1166, 1349
AvaI CYCGRG 3 cut(s) 314, 513, 1206
AvaII GGWCC 4 cut(s) 166, 291, 499, 1047
AvrII CCTAGG 1 cut(s) 1235
BaeGI GKGCMC 3 cut(s) 400, 639, 1234
BanI GGYRCC 2 cut(s) 128, 322
BanII GRGCYC 3 cut(s) 400, 520, 639
BauI CACGAG 1 cut(s) 1144
BbsI GAAGAC 2 cut(s) 706, 885
Bbv12I GWGCWC 4 cut(s) 283, 409, 786, 1145
BbvCI CCTCAGC 2 cut(s) 222, 255
BbvI GCAGC 6 cut(s) 14, 136, 214, 224, 362, 1049
BccI CCATC 5 cut(s) 153, 727, 758, 943, 1328
BceAI ACGGC 5 cut(s) 200, 225, 338, 642, 719
BciT130I CCWGG 3 cut(s) 170, 386, 1092
BciVI GTATCC 1 cut(s) 593
BcnI CCSGG 3 cut(s) 288, 315, 316
BfaI CTAG 4 cut(s) 525, 546, 692, 1236
BfmI CTRYAG 3 cut(s) 195, 647, 1084
BfoI RGCGCY 1 cut(s) 326
BfuI GTATCC 1 cut(s) 593
BglI GCCNNNNNGGC 1 cut(s) 209
BlnI CCTAGG 1 cut(s) 1235
Bme1390I CCNGG 6 cut(s) 170, 288, 315, 316, 386, 1092
Bme18I GGWCC 4 cut(s) 166, 291, 499, 1047
BmeT110I CYCGRG 3 cut(s) 314, 513, 1206
BmgT120I GGNCC 8 cut(s) 166, 291, 396, 397, 499, 635, 636, 1047
BmiI GGNNCC 6 cut(s) 130, 292, 324, 398, 637, 847
BmrFI CCNGG 6 cut(s) 170, 288, 315, 316, 386, 1092
BmsI GCATC 3 cut(s) 136, 644, 719
BoxI GACNNNNGTC 1 cut(s) 497
BpiI GAAGAC 2 cut(s) 706, 885
BpmI CTGGAG 1 cut(s) 1351
Bpu10I CCTNAGC 4 cut(s) 222, 255, 479, 1212
BpuEI CTTGAG 2 cut(s) 138, 1268
BpuMI CCSGG 3 cut(s) 288, 315, 316
BsaHI GRCGYC 3 cut(s) 174, 323, 681
BsaJI CCNNGG 5 cut(s) 287, 314, 315, 1020, 1235
BsaWI WCCGGW 1 cut(s) 1285
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 733, 842, 1213, 1401
Bse1I ACTGG 5 cut(s) 423, 501, 601, 727, 1170
BseAI TCCGGA 1 cut(s) 1285
BseBI CCWGG 3 cut(s) 170, 386, 1092
BseDI CCNNGG 5 cut(s) 287, 314, 315, 1020, 1235
BseGI GGATG 4 cut(s) 164, 738, 1036, 1202
BseLI CCNNNNNNNGG 5 cut(s) 316, 733, 842, 1213, 1401
BseMII CTCAG 6 cut(s) 213, 246, 392, 493, 764, 801
BseNI ACTGG 5 cut(s) 423, 501, 601, 727, 1170
BseRI GAGGAG 1 cut(s) 286
BseSI GKGCMC 3 cut(s) 400, 639, 1234
BseXI GCAGC 6 cut(s) 14, 136, 214, 224, 362, 1049
BseYI CCCAGC 1 cut(s) 862
Bsh1236I CGCG 5 cut(s) 137, 188, 236, 612, 885
BshFI GGCC 3 cut(s) 398, 447, 637
BshNI GGYRCC 2 cut(s) 128, 322
BsiHKAI GWGCWC 4 cut(s) 283, 409, 786, 1145
BsiHKCI CYCGRG 3 cut(s) 314, 513, 1206
BsiSI CCGG 4 cut(s) 287, 315, 376, 1286
BslFI GGGAC 3 cut(s) 304, 692, 764
BslI CCNNNNNNNGG 5 cut(s) 316, 733, 842, 1213, 1401
BsmFI GGGAC 3 cut(s) 304, 692, 764
BsmI GAATGC 2 cut(s) 541, 1303
BsnI GGCC 3 cut(s) 398, 447, 637
BsoBI CYCGRG 3 cut(s) 314, 513, 1206
Bsp120I GGGCCC 2 cut(s) 396, 635
Bsp1286I GDGCHC 8 cut(s) 283, 400, 409, 520, 639, 786, 1145, 1234
Bsp13I TCCGGA 1 cut(s) 1285
Bsp143I GATC 3 cut(s) 568, 898, 956
BspANI GGCC 3 cut(s) 398, 447, 637
BspCNI CTCAG 6 cut(s) 214, 247, 393, 492, 765, 800
BspEI TCCGGA 1 cut(s) 1285
BspFNI CGCG 5 cut(s) 137, 188, 236, 612, 885
BspLI GGNNCC 6 cut(s) 130, 292, 324, 398, 637, 847
BspMAI CTGCAG 1 cut(s) 651
BspPI GGATC 2 cut(s) 576, 964
BspT107I GGYRCC 2 cut(s) 128, 322
BsrI ACTGG 5 cut(s) 423, 501, 601, 727, 1170
BssECI CCNNGG 5 cut(s) 287, 314, 315, 1020, 1235
BssMI GATC 3 cut(s) 568, 898, 956
BssNI GRCGYC 3 cut(s) 174, 323, 681
BssSI CACGAG 1 cut(s) 1144
BssT1I CCWWGG 2 cut(s) 1020, 1235
Bst2BI CACGAG 1 cut(s) 1144
Bst2UI CCWGG 3 cut(s) 170, 386, 1092
Bst4CI ACNGT 1 cut(s) 366
Bst6I CTCTTC 2 cut(s) 26, 1102
BstACI GRCGYC 3 cut(s) 174, 323, 681
BstAPI GCANNNNNTGC 2 cut(s) 149, 1076
BstC8I GCNNGC 4 cut(s) 186, 311, 708, 1128
BstDEI CTNAG 7 cut(s) 222, 255, 401, 479, 773, 787, 1212
BstEII GGTNACC 1 cut(s) 1337
BstF5I GGATG 4 cut(s) 164, 738, 1036, 1202
BstFNI CGCG 5 cut(s) 137, 188, 236, 612, 885
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 3 cut(s) 571, 901, 959
BstMBI GATC 3 cut(s) 568, 898, 956
BstNI CCWGG 3 cut(s) 170, 386, 1092
BstNSI RCATGY 1 cut(s) 478
BstPAI GACNNNNGTC 1 cut(s) 497
BstPI GGTNACC 1 cut(s) 1337
BstSCI CCNGG 6 cut(s) 168, 286, 313, 314, 384, 1090
BstSFI CTRYAG 3 cut(s) 195, 647, 1084
BstSLI GKGCMC 3 cut(s) 400, 639, 1234
BstUI CGCG 5 cut(s) 137, 188, 236, 612, 885
BstV1I GCAGC 6 cut(s) 14, 136, 214, 224, 362, 1049
BstV2I GAAGAC 2 cut(s) 706, 885
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 3 cut(s) 398, 447, 637
BtgZI GCGATG 1 cut(s) 866
BtsCI GGATG 4 cut(s) 164, 738, 1036, 1202
BtsI GCAGTG 1 cut(s) 1105
BtsIMutI CAGTG 4 cut(s) 416, 494, 734, 1105
Cac8I GCNNGC 4 cut(s) 186, 311, 708, 1128
CaiI CAGNNNCTG 2 cut(s) 149, 496
CfoI GCGC 1 cut(s) 325
Cfr13I GGNCC 8 cut(s) 166, 291, 396, 397, 499, 635, 636, 1047
Cfr9I CCCGGG 1 cut(s) 314
CseI GACGC 3 cut(s) 126, 182, 689
Csp6I GTAC 1 cut(s) 744
CviAII CATG 5 cut(s) 245, 475, 538, 747, 1186
CviQI GTAC 1 cut(s) 744
DdeI CTNAG 7 cut(s) 222, 255, 401, 479, 773, 787, 1212
DinI GGCGCC 1 cut(s) 324
DpnI GATC 3 cut(s) 570, 900, 958
DpnII GATC 3 cut(s) 568, 898, 956
DraIII CACNNNGTG 1 cut(s) 1229
DrdI GACNNNNNNGTC 1 cut(s) 1276
DseDI GACNNNNNNGTC 1 cut(s) 1276
Eam1104I CTCTTC 2 cut(s) 26, 1102
EarI CTCTTC 2 cut(s) 26, 1102
EciI GGCGGA 1 cut(s) 179
Eco130I CCWWGG 2 cut(s) 1020, 1235
Eco24I GRGCYC 3 cut(s) 400, 520, 639
Eco32I GATATC 1 cut(s) 1007
Eco47I GGWCC 4 cut(s) 166, 291, 499, 1047
Eco57I CTGAAG 2 cut(s) 879, 1350
Eco88I CYCGRG 3 cut(s) 314, 513, 1206
Eco91I GGTNACC 1 cut(s) 1337
EcoO109I RGGNCCY 4 cut(s) 291, 396, 397, 636
EcoO65I GGTNACC 1 cut(s) 1337
EcoRII CCWGG 3 cut(s) 168, 384, 1090
EcoRV GATATC 1 cut(s) 1007
EcoT14I CCWWGG 2 cut(s) 1020, 1235
EcoT22I ATGCAT 1 cut(s) 543
EcoT38I GRGCYC 3 cut(s) 400, 520, 639
EgeI GGCGCC 1 cut(s) 324
EheI GGCGCC 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 1020, 1235
FaeI CATG 5 cut(s) 248, 478, 541, 750, 1189
FalI AAGNNNNNCTT 4 cut(s) 824, 856, 964, 996
FaqI GGGAC 3 cut(s) 304, 692, 764
FatI CATG 5 cut(s) 244, 474, 537, 746, 1185
FauI CCCGC 1 cut(s) 582
FauNDI CATATG 1 cut(s) 925
FblI GTMKAC 2 cut(s) 856, 1087
FokI GGATG 4 cut(s) 171, 745, 1043, 1189
FriOI GRGCYC 3 cut(s) 400, 520, 639
FspBI CTAG 4 cut(s) 525, 546, 692, 1236
GlaI GCGC 1 cut(s) 324
GsaI CCCAGC 1 cut(s) 866
GsuI CTGGAG 1 cut(s) 1351
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 3 cut(s) 398, 447, 637
HapII CCGG 4 cut(s) 287, 315, 376, 1286
HgaI GACGC 3 cut(s) 126, 182, 689
HhaI GCGC 1 cut(s) 325
Hin1I GRCGYC 3 cut(s) 174, 323, 681
Hin1II CATG 5 cut(s) 248, 478, 541, 750, 1189
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 3 cut(s) 425, 432, 978
HinfI GANTC 6 cut(s) 18, 85, 504, 929, 1199, 1289
HpaII CCGG 4 cut(s) 287, 315, 376, 1286
HphI GGTGA 5 cut(s) 433, 451, 563, 1166, 1349
Hpy166II GTNNAC 3 cut(s) 805, 857, 1088
Hpy188I TCNGA 9 cut(s) 57, 338, 503, 568, 790, 898, 1011, 1315, 1346
Hpy188III TCNNGA 4 cut(s) 692, 815, 1206, 1286
Hpy8I GTNNAC 3 cut(s) 805, 857, 1088
Hpy99I CGWCG 2 cut(s) 221, 342
HpyAV CCTTC 4 cut(s) 336, 387, 734, 793
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 2 cut(s) 672, 853
HpyCH4V TGCA 5 cut(s) 30, 490, 541, 649, 1301
HpyF3I CTNAG 7 cut(s) 222, 255, 401, 479, 773, 787, 1212
HpySE526I ACGT 2 cut(s) 672, 853
Hsp92I GRCGYC 3 cut(s) 174, 323, 681
Hsp92II CATG 5 cut(s) 248, 478, 541, 750, 1189
HspAI GCGC 1 cut(s) 323
KasI GGCGCC 1 cut(s) 322
Kpn2I TCCGGA 1 cut(s) 1285
Kzo9I GATC 3 cut(s) 568, 898, 956
LmnI GCTCC 3 cut(s) 286, 1370, 1397
Lsp1109I GCAGC 6 cut(s) 14, 136, 214, 224, 362, 1049
LweI GCATC 3 cut(s) 136, 644, 719
MaeI CTAG 4 cut(s) 525, 546, 692, 1236
MaeII ACGT 2 cut(s) 672, 853
MaeIII GTNAC 3 cut(s) 1172, 1221, 1337
MalI GATC 3 cut(s) 570, 900, 958
MboI GATC 3 cut(s) 568, 898, 956
MboII GAAGA 7 cut(s) 13, 370, 711, 749, 885, 1119, 1343
MhlI GDGCHC 8 cut(s) 283, 400, 409, 520, 639, 786, 1145, 1234
MluCI AATT 3 cut(s) 626, 664, 810
Mly113I GGCGCC 1 cut(s) 323
MlyI GAGTC 5 cut(s) 12, 94, 513, 923, 1298
MmeI TCCRAC 3 cut(s) 80, 361, 1366
Mph1103I ATGCAT 1 cut(s) 543
MroI TCCGGA 1 cut(s) 1285
MroXI GAANNNNTTC 1 cut(s) 1309
MseI TTAA 1 cut(s) 1158
MslI CAYNNNNRTG 1 cut(s) 789
MspI CCGG 4 cut(s) 287, 315, 376, 1286
MspR9I CCNGG 6 cut(s) 170, 288, 315, 316, 386, 1092
Mva1269I GAATGC 2 cut(s) 541, 1303
MvaI CCWGG 3 cut(s) 170, 386, 1092
MvnI CGCG 5 cut(s) 137, 188, 236, 612, 885
NarI GGCGCC 1 cut(s) 323
NciI CCSGG 3 cut(s) 288, 315, 316
NdeI CATATG 1 cut(s) 925
NdeII GATC 3 cut(s) 568, 898, 956
NlaIII CATG 5 cut(s) 248, 478, 541, 750, 1189
NlaIV GGNNCC 6 cut(s) 130, 292, 324, 398, 637, 847
NmeAIII GCCGAG 1 cut(s) 785
NmuCI GTSAC 3 cut(s) 1172, 1221, 1337
NsiI ATGCAT 1 cut(s) 543
NspI RCATGY 1 cut(s) 478
PctI GAATGC 2 cut(s) 541, 1303
PdmI GAANNNNTTC 1 cut(s) 1309
PfeI GAWTC 1 cut(s) 1199
PflMI CCANNNNNTGG 1 cut(s) 733
PleI GAGTC 5 cut(s) 12, 93, 512, 923, 1297
PluTI GGCGCC 1 cut(s) 326
PpsI GAGTC 5 cut(s) 12, 93, 512, 923, 1297
PpuMI RGGWCCY 1 cut(s) 291
PshAI GACNNNNGTC 1 cut(s) 497
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 3 cut(s) 168, 384, 1090
PspEI GGTNACC 1 cut(s) 1337
PspFI CCCAGC 1 cut(s) 862
PspGI CCWGG 3 cut(s) 168, 384, 1090
PspN4I GGNNCC 6 cut(s) 130, 292, 324, 398, 637, 847
PspOMI GGGCCC 2 cut(s) 396, 635
PspPI GGNCC 8 cut(s) 166, 291, 396, 397, 499, 635, 636, 1047
PspPPI RGGWCCY 1 cut(s) 291
PstI CTGCAG 1 cut(s) 651
PstNI CAGNNNCTG 2 cut(s) 149, 496
RsaI GTAC 1 cut(s) 745
RsaNI GTAC 1 cut(s) 744
RseI CAYNNNNRTG 1 cut(s) 789
SaqAI TTAA 1 cut(s) 1158
Sau3AI GATC 3 cut(s) 568, 898, 956
Sau96I GGNCC 8 cut(s) 166, 291, 396, 397, 499, 635, 636, 1047
SchI GAGTC 5 cut(s) 12, 94, 513, 923, 1298
ScrFI CCNGG 6 cut(s) 170, 288, 315, 316, 386, 1092
SduI GDGCHC 8 cut(s) 283, 400, 409, 520, 639, 786, 1145, 1234
SfaNI GCATC 3 cut(s) 136, 644, 719
SfcI CTRYAG 3 cut(s) 195, 647, 1084
SfoI GGCGCC 1 cut(s) 324
SinI GGWCC 4 cut(s) 166, 291, 499, 1047
SmaI CCCGGG 1 cut(s) 316
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 2 cut(s) 153, 1247
SmoI CTYRAG 2 cut(s) 153, 1247
Sse9I AATT 3 cut(s) 626, 664, 810
SspDI GGCGCC 1 cut(s) 322
SspMI CTAG 4 cut(s) 525, 546, 692, 1236
StyD4I CCNGG 6 cut(s) 168, 286, 313, 314, 384, 1090
StyI CCWWGG 2 cut(s) 1020, 1235
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 2 cut(s) 675, 856
TaqI TCGA 1 cut(s) 947
TasI AATT 3 cut(s) 626, 664, 810
TfiI GAWTC 1 cut(s) 1199
Tru1I TTAA 1 cut(s) 1158
Tru9I TTAA 1 cut(s) 1158
TscAI CASTG 4 cut(s) 423, 501, 734, 1105
TseFI GTSAC 3 cut(s) 1172, 1221, 1337
TseI GCWGC 6 cut(s) 27, 149, 212, 227, 350, 1037
Tsp45I GTSAC 3 cut(s) 1172, 1221, 1337
TspDTI ATGAA 1 cut(s) 1047
TspGWI ACGGA 2 cut(s) 1043, 1211
TspMI CCCGGG 1 cut(s) 314
TspRI CASTG 4 cut(s) 423, 501, 734, 1105
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 4 cut(s) 166, 291, 499, 1047
XapI RAATTY 3 cut(s) 626, 664, 810
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 1 cut(s) 478
XmaI CCCGGG 1 cut(s) 314
XmaJI CCTAGG 1 cut(s) 1235
XmiI GTMKAC 2 cut(s) 856, 1087
XmnI GAANNNNTTC 1 cut(s) 1309
XspI CTAG 4 cut(s) 525, 546, 692, 1236
Zsp2I ATGCAT 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.